Click here to switch to the map view.

The map label for this gene is mrcB [H]

Identifier: 123968019

GI number: 123968019

Start: 417399

End: 419168

Strand: Reverse

Name: mrcB [H]

Synonym: A9601_04821

Alternate gene names: 123968019

Gene position: 419168-417399 (Counterclockwise)

Preceding gene: 123968020

Following gene: 123968010

Centisome position: 25.1

GC content: 26.84

Gene sequence:

>1770_bases
GTGCAAAAGATTAAATTCAAATCTTTTGTTTTAATAATTCCAATATTAATTTTTTCTGGAATATCTTTTTATTTTTTTAG
TCTAATATTTAATATCTTAAAATTTGACGTTTCTAAAAATAAAAAGTCTCAGGAAACCAAATATTCTTACGTAATATCAT
CTTCTGATAATAAGATACTAAGTAAATTAAGCCGCAAATTTGAAATAGATAATAGTTATGATAAAATTCCATTTTTTCTT
AAACATTCTTTTATATCTTCCGAGGATAAAAGATTTTATAAACATAATGGAATAGATTTAAAAAGTATTTCACGTGCCCT
TATTCAAAATTTTAGAAGTGGTTATGTTAAAGAGGGAGGAAGTACGATTACACAACAAGTTTCTAGATTACTTTTTCTAA
ATAATGATTTAAGTTTTCAAAGAAAAATCAAAGAAATATTGATATCACTCATACTTGAATTTAGATATAACAAAAATCAA
ATTTTAAAATTATATTTAAACAATATTTATCTAGGATCAGGAGCATACGGGGTAAACGAGGCTGCCCAAGTTTATTTTGG
AAAATTTGTAGAAGAATTGACATTATCAGAGATCGCATTAATTGCAGGATTAGCTCCAGCTCCATCAATTTATTCACCTT
ATCAAAATTTAGAACTAGCTATTAAAAATAGAAATAAAGTTCTTGAATCAATGTATGTTGATGGATATATTTCTCTTGCA
AATAAGGATAAGGCTATTAAAGAAAAAATAGATTTAAATTATCAAAAAGCTGATAATTTTTTAGATGATAAATTACTAAT
AAAATTTATTCTTCAGGAAACAGATAAAAAAATTGGAAGTAAAAATGATTATAAGTTTTTAAGAATTAAATCTTCTATCA
ACAAAGATTGGCAAGAAAAAGGTCAAAAAATATCAACATACGCAGGGCCTAAAGAACTTGAATTTGGTCTGTTATCTATC
GAATCAAACACAGGACTAATCAGGACAATGATAACCAGCAAAAATCCATCAATTAATGAATACAATAGAGTGATTTCATC
TGTAAGACCTTTAGGTTCTACTTTTAAAATAATCCCTTATGCTGCTGCATTAATAGAAGGAACAAAATTAAGCGATAAAT
TTGAAGACTTACCAATATGCTGGGAAAGTTATTGCCCAAAAAATTTTTCAGAAGACTATAGAGGTTCAATATCTTTGATT
GAATCATTTAAAAGTTCATCAAATATCGTTCCAATATCAATAACAAAAACAATCGGCTTAAAAAATATAATTAATTTAGC
GAATTCATTTGGACTTGGTTACGAGCAAGAGTTTGAGGAATTCCCATCATTAGCTATTGGCGCCTACGGAGATAATCTTT
TAAACATCACAAATGCATATTCTGCAATAAACAATAATGGGAAAATTCAAAGCACTGAAATCATAGAAAAAATAGAATCA
TTTAAAAAACAACCTATTTGGGAAAATAAATCTATTTCCAAGAAAATATTAGATTTAAAAATAAACAAGAAAATAAATAA
ACTTCTTGAAAAATCTGTAAAAGAAGGCACTTCAAAAGCAGCCTCTATAAAAGGAAATAAAATTTATGGGAAAACAGGAA
CATCTGATGGGAATAAAGATCTCTGGTTTATTGGTTCCATTGATAACCTTACAACAGGGATCTGGATAGGTTATGACGAT
AACAAGGAAACTGAATTATCTAGTGGGAATGCAGCTTATTTATGGAAGAAGTTCATATCTGAAATTTATAAAATTCAAAT
TAAAAAATAA

Upstream 100 bases:

>100_bases
AAGTTTGATGTCAAATATCAAGCAAGTGCCCAACCGTTCCTTATAACTGGGATGTTAGTTACAGCTTTAGCGATAGGACA
TAGTTTTTTAGTTGCTTAAG

Downstream 100 bases:

>100_bases
ATTATATTTTTAAGATTTATAAGAAATTATTGCAGAATAAAATCCATCACCAGGATAATCCAAGCTAGGTAAAATTTGCT
TTTGGCTAACCAATTTTAAA

Product: putative penicillin binding protein

Products: NA

Alternate protein names: PBP 4 [H]

Number of amino acids: Translated: 589; Mature: 589

Protein sequence:

>589_residues
MQKIKFKSFVLIIPILIFSGISFYFFSLIFNILKFDVSKNKKSQETKYSYVISSSDNKILSKLSRKFEIDNSYDKIPFFL
KHSFISSEDKRFYKHNGIDLKSISRALIQNFRSGYVKEGGSTITQQVSRLLFLNNDLSFQRKIKEILISLILEFRYNKNQ
ILKLYLNNIYLGSGAYGVNEAAQVYFGKFVEELTLSEIALIAGLAPAPSIYSPYQNLELAIKNRNKVLESMYVDGYISLA
NKDKAIKEKIDLNYQKADNFLDDKLLIKFILQETDKKIGSKNDYKFLRIKSSINKDWQEKGQKISTYAGPKELEFGLLSI
ESNTGLIRTMITSKNPSINEYNRVISSVRPLGSTFKIIPYAAALIEGTKLSDKFEDLPICWESYCPKNFSEDYRGSISLI
ESFKSSSNIVPISITKTIGLKNIINLANSFGLGYEQEFEEFPSLAIGAYGDNLLNITNAYSAINNNGKIQSTEIIEKIES
FKKQPIWENKSISKKILDLKINKKINKLLEKSVKEGTSKAASIKGNKIYGKTGTSDGNKDLWFIGSIDNLTTGIWIGYDD
NKETELSSGNAAYLWKKFISEIYKIQIKK

Sequences:

>Translated_589_residues
MQKIKFKSFVLIIPILIFSGISFYFFSLIFNILKFDVSKNKKSQETKYSYVISSSDNKILSKLSRKFEIDNSYDKIPFFL
KHSFISSEDKRFYKHNGIDLKSISRALIQNFRSGYVKEGGSTITQQVSRLLFLNNDLSFQRKIKEILISLILEFRYNKNQ
ILKLYLNNIYLGSGAYGVNEAAQVYFGKFVEELTLSEIALIAGLAPAPSIYSPYQNLELAIKNRNKVLESMYVDGYISLA
NKDKAIKEKIDLNYQKADNFLDDKLLIKFILQETDKKIGSKNDYKFLRIKSSINKDWQEKGQKISTYAGPKELEFGLLSI
ESNTGLIRTMITSKNPSINEYNRVISSVRPLGSTFKIIPYAAALIEGTKLSDKFEDLPICWESYCPKNFSEDYRGSISLI
ESFKSSSNIVPISITKTIGLKNIINLANSFGLGYEQEFEEFPSLAIGAYGDNLLNITNAYSAINNNGKIQSTEIIEKIES
FKKQPIWENKSISKKILDLKINKKINKLLEKSVKEGTSKAASIKGNKIYGKTGTSDGNKDLWFIGSIDNLTTGIWIGYDD
NKETELSSGNAAYLWKKFISEIYKIQIKK
>Mature_589_residues
MQKIKFKSFVLIIPILIFSGISFYFFSLIFNILKFDVSKNKKSQETKYSYVISSSDNKILSKLSRKFEIDNSYDKIPFFL
KHSFISSEDKRFYKHNGIDLKSISRALIQNFRSGYVKEGGSTITQQVSRLLFLNNDLSFQRKIKEILISLILEFRYNKNQ
ILKLYLNNIYLGSGAYGVNEAAQVYFGKFVEELTLSEIALIAGLAPAPSIYSPYQNLELAIKNRNKVLESMYVDGYISLA
NKDKAIKEKIDLNYQKADNFLDDKLLIKFILQETDKKIGSKNDYKFLRIKSSINKDWQEKGQKISTYAGPKELEFGLLSI
ESNTGLIRTMITSKNPSINEYNRVISSVRPLGSTFKIIPYAAALIEGTKLSDKFEDLPICWESYCPKNFSEDYRGSISLI
ESFKSSSNIVPISITKTIGLKNIINLANSFGLGYEQEFEEFPSLAIGAYGDNLLNITNAYSAINNNGKIQSTEIIEKIES
FKKQPIWENKSISKKILDLKINKKINKLLEKSVKEGTSKAASIKGNKIYGKTGTSDGNKDLWFIGSIDNLTTGIWIGYDD
NKETELSSGNAAYLWKKFISEIYKIQIKK

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=529, Percent_Identity=25.8979206049149, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI87082258, Length=303, Percent_Identity=35.973597359736, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI1788867, Length=497, Percent_Identity=28.3702213279678, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1789601, Length=137, Percent_Identity=40.8759124087591, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 67011; Mature: 67011

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKIKFKSFVLIIPILIFSGISFYFFSLIFNILKFDVSKNKKSQETKYSYVISSSDNKIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHH
SKLSRKFEIDNSYDKIPFFLKHSFISSEDKRFYKHNGIDLKSISRALIQNFRSGYVKEGG
HHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCHHCCCC
STITQQVSRLLFLNNDLSFQRKIKEILISLILEFRYNKNQILKLYLNNIYLGSGAYGVNE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCCH
AAQVYFGKFVEELTLSEIALIAGLAPAPSIYSPYQNLELAIKNRNKVLESMYVDGYISLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCEEEEC
NKDKAIKEKIDLNYQKADNFLDDKLLIKFILQETDKKIGSKNDYKFLRIKSSINKDWQEK
CCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHC
GQKISTYAGPKELEFGLLSIESNTGLIRTMITSKNPSINEYNRVISSVRPLGSTFKIIPY
CCEEECCCCCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHH
AAALIEGTKLSDKFEDLPICWESYCPKNFSEDYRGSISLIESFKSSSNIVPISITKTIGL
HHHHHCCCCCCCHHCCCCCHHHHCCCCCCCHHHCCHHHHHHHHHCCCCEEEEEEEHHHHH
KNIINLANSFGLGYEQEFEEFPSLAIGAYGDNLLNITNAYSAINNNGKIQSTEIIEKIES
HHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
FKKQPIWENKSISKKILDLKINKKINKLLEKSVKEGTSKAASIKGNKIYGKTGTSDGNKD
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEECCCCCCCCCCC
LWFIGSIDNLTTGIWIGYDDNKETELSSGNAAYLWKKFISEIYKIQIKK
EEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MQKIKFKSFVLIIPILIFSGISFYFFSLIFNILKFDVSKNKKSQETKYSYVISSSDNKIL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHH
SKLSRKFEIDNSYDKIPFFLKHSFISSEDKRFYKHNGIDLKSISRALIQNFRSGYVKEGG
HHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCHHCCCC
STITQQVSRLLFLNNDLSFQRKIKEILISLILEFRYNKNQILKLYLNNIYLGSGAYGVNE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEECCCCCCCCH
AAQVYFGKFVEELTLSEIALIAGLAPAPSIYSPYQNLELAIKNRNKVLESMYVDGYISLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCEEEEC
NKDKAIKEKIDLNYQKADNFLDDKLLIKFILQETDKKIGSKNDYKFLRIKSSINKDWQEK
CCCHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHC
GQKISTYAGPKELEFGLLSIESNTGLIRTMITSKNPSINEYNRVISSVRPLGSTFKIIPY
CCEEECCCCCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHH
AAALIEGTKLSDKFEDLPICWESYCPKNFSEDYRGSISLIESFKSSSNIVPISITKTIGL
HHHHHCCCCCCCHHCCCCCHHHHCCCCCCCHHHCCHHHHHHHHHCCCCEEEEEEEHHHHH
KNIINLANSFGLGYEQEFEEFPSLAIGAYGDNLLNITNAYSAINNNGKIQSTEIIEKIES
HHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
FKKQPIWENKSISKKILDLKINKKINKLLEKSVKEGTSKAASIKGNKIYGKTGTSDGNKD
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCEEECCCCCCCCCCC
LWFIGSIDNLTTGIWIGYDDNKETELSSGNAAYLWKKFISEIYKIQIKK
EEEEECCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961491; 9274030; 9384377 [H]