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The map label for this gene is trmH [H]

Identifier: 123968010

GI number: 123968010

Start: 412173

End: 412844

Strand: Reverse

Name: trmH [H]

Synonym: A9601_04731

Alternate gene names: 123968010

Gene position: 412844-412173 (Counterclockwise)

Preceding gene: 123968019

Following gene: 123968006

Centisome position: 24.72

GC content: 31.4

Gene sequence:

>672_bases
ATGTCAATTTTGCCAAGAAGATTTGAACGAATCAAAAGTGTTTTAGATTGCAGAATGAAAAACTTGACTGTTTTAGTTGA
GGATGTCAATAAACCACATAATTTATCTGCGATATTAAGGACATGTGATGCAGCAGGAGTTTTCGAAGCAAATTTTATTA
GCAAAACGAATGCCGTTAAGACTTTTAATAGTACTGCGCAAGGAAGTCAAAAATGGGTAAAACTGAATAATCATGAAAAC
ACTATCACAGCAATATCTGATTTAAAGAATAAGGGTTTTAAATTATATGGAACGACTCTTAATAGTGAATCAGTAGATTA
TAGAAATTTTGATTATTCTCAAAATACATGTTTTGTTCTAGGAGCAGAAAAATGGGGACTAAGTAATGAACTTATATCAA
TGGTTGATCAATCAATTTTTATACCTATGAGAGGTATGGTTCAATCTCTGAATGTTTCAGTTGCTGCTTCTATATTATTA
TTTGAAGCTGTTCGCCAAAGAAAAAATAAAGGTATATTGCCCGCTAATGGAGAAGGGTTAAATATGGATGAATATCAAAA
AACACTTTTTGAATGGTGTTACCCAGAATTAGCTGAGGTGTATAAAAAATCAGCTATAGAATATCCAAAGTTGAATGATC
AAGGAGAACTTGATCCTATTACAGATAACTAA

Upstream 100 bases:

>100_bases
CCATTGAGTTAATTTAAACTACCAAAATATTATAAACCAAGAGATTTAATAGGTAATCAATATTTTTTGAATATACTTTA
AAAGTGTGAAAAAATTATAA

Downstream 100 bases:

>100_bases
ATTTATACAGAATTTTGACTATCAAAAATTTCTTCAAGGTCCTCTCCTATAAAAGAAAGACCTAAAACTAAAAAAAACAT
TGCCAAACCTGGGAACAAAG

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MSILPRRFERIKSVLDCRMKNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVKTFNSTAQGSQKWVKLNNHEN
TITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVLGAEKWGLSNELISMVDQSIFIPMRGMVQSLNVSVAASILL
FEAVRQRKNKGILPANGEGLNMDEYQKTLFEWCYPELAEVYKKSAIEYPKLNDQGELDPITDN

Sequences:

>Translated_223_residues
MSILPRRFERIKSVLDCRMKNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVKTFNSTAQGSQKWVKLNNHEN
TITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVLGAEKWGLSNELISMVDQSIFIPMRGMVQSLNVSVAASILL
FEAVRQRKNKGILPANGEGLNMDEYQKTLFEWCYPELAEVYKKSAIEYPKLNDQGELDPITDN
>Mature_222_residues
SILPRRFERIKSVLDCRMKNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVKTFNSTAQGSQKWVKLNNHENT
ITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVLGAEKWGLSNELISMVDQSIFIPMRGMVQSLNVSVAASILLF
EAVRQRKNKGILPANGEGLNMDEYQKTLFEWCYPELAEVYKKSAIEYPKLNDQGELDPITDN

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI110825988, Length=155, Percent_Identity=26.4516129032258, Blast_Score=72, Evalue=3e-13,
Organism=Escherichia coli, GI1790083, Length=218, Percent_Identity=43.5779816513761, Blast_Score=202, Evalue=1e-53,
Organism=Escherichia coli, GI1790623, Length=148, Percent_Identity=36.4864864864865, Blast_Score=79, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI193204953, Length=157, Percent_Identity=29.2993630573248, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21355819, Length=151, Percent_Identity=29.1390728476821, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25203; Mature: 25072

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILPRRFERIKSVLDCRMKNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVK
CCCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCCHHH
TFNSTAQGSQKWVKLNNHENTITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVL
HCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEE
GAEKWGLSNELISMVDQSIFIPMRGMVQSLNVSVAASILLFEAVRQRKNKGILPANGEGL
ECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NMDEYQKTLFEWCYPELAEVYKKSAIEYPKLNDQGELDPITDN
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SILPRRFERIKSVLDCRMKNLTVLVEDVNKPHNLSAILRTCDAAGVFEANFISKTNAVK
CCCHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHCCCCHHH
TFNSTAQGSQKWVKLNNHENTITAISDLKNKGFKLYGTTLNSESVDYRNFDYSQNTCFVL
HCCCCCCCCCCEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEE
GAEKWGLSNELISMVDQSIFIPMRGMVQSLNVSVAASILLFEAVRQRKNKGILPANGEGL
ECCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NMDEYQKTLFEWCYPELAEVYKKSAIEYPKLNDQGELDPITDN
CHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]