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The map label for this gene is metB [H]

Identifier: 123967996

GI number: 123967996

Start: 399929

End: 401092

Strand: Reverse

Name: metB [H]

Synonym: A9601_04591

Alternate gene names: 123967996

Gene position: 401092-399929 (Counterclockwise)

Preceding gene: 123967997

Following gene: 123967995

Centisome position: 24.02

GC content: 33.33

Gene sequence:

>1164_bases
ATGGGAAATAAGGAAAATAATATAAAAAAGCCAGGTTTTAAGACCTTATCTATTCACCATGGGGAAACATTTGCTGAAGA
AACTGGATGCGTTATGCCTCCTATTTTTTCTACATCTACTTTCAAACATGGAAATAAAGATAATTTCGACTACACCAGAT
CAGGCAATCCAAACTTTAAAATTCTAGAAAACATCCTTAAATCAATAGAAGATTCTAAATACTGTACAGTTTTTGGATCT
GGAATTAGCGCGGTAACTGCAATTTCATCAACACTGAAATCAGGTGACAAGATACTCTGCGAGTCAAATCTCTATGGTTG
TACAGTGAGGATGTTCGAAAAAGTTTTCAAGAAATTTGGACTAGAAATTTTATACACAGATTTTACAAACGAAAATAATG
TCAAAAAGATTTCAAACTTCGAGCCAACCTTGATATGGCTAGAAAGTCCAACTAATCCACTTTTAAAGGTACTCGATATT
AAGGCGATTTGTGATGAAGCGAATAAACTAGAAATACCAGTAGTTGTAGACAACACATTTTCTACAGCGCTTATTCAAAA
ACCATTGGACCTTGGTGCAACACTATCGGTTGTAAGCACCACAAAATTCATTAATGGACATAGTGACGCACTTGGCGGAG
CAGTACTTACAAATAATGAAGAATGGAATAGTAAGATGCTTTTCTCTCAAAAAGCTCTCGGGCTTCAACCATCTCCTTTT
GATAGTTGGCTCATTACGAGAGGAGTAAAAACTCTTCCTTTAAGAATCGAACAACAAACTAAAAGTGCAGAATTTATTTC
TGAAGAATTAGGTAATCATAAAATTATTAGTAAATTAATTTACCCTTTTAATCAAGAACATCCGCAATTTAATTTAGCAA
AATCGCAAATGAAATCTGGAGGTTCAATGATCAGCCTAAAATTAAATTTAAATAAAGAGGATACTTTTAAATTTTGCAAA
TCTCTCAAATATTTCTCTCTAGCAGAAAGTCTTGGAGGAGTTGAAAGTTTAATTTGTCACCCTGCAACGATGACTCATGC
TTCTGTTGATGACAAAACAAAAAATCTACTAGGGATAGATGATGCTCTTGTCAGATTATCAATTGGATGTGAAGATACAA
ACGATTTAATCTCGGACATTTTATTTGCTTTAAATAAATTCTAA

Upstream 100 bases:

>100_bases
GAATATTATTCAAGAAAAGTTTAATACTACTTTTCTTTGGATTATTAAATCTCTCATTTTTTAAAAGAGAGATTTAATTT
AATTCTTATTTTTAAAAATA

Downstream 100 bases:

>100_bases
AAATTGAGAGATTTACTTAAAAAACCTATATGGAAAAATTTAGAGTTGGGATATGCAATTCCTGATAGTATTCATGCTGT
TTCTGTAGCATTACCAACTT

Product: putative cystathionine gamma-synthase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MGNKENNIKKPGFKTLSIHHGETFAEETGCVMPPIFSTSTFKHGNKDNFDYTRSGNPNFKILENILKSIEDSKYCTVFGS
GISAVTAISSTLKSGDKILCESNLYGCTVRMFEKVFKKFGLEILYTDFTNENNVKKISNFEPTLIWLESPTNPLLKVLDI
KAICDEANKLEIPVVVDNTFSTALIQKPLDLGATLSVVSTTKFINGHSDALGGAVLTNNEEWNSKMLFSQKALGLQPSPF
DSWLITRGVKTLPLRIEQQTKSAEFISEELGNHKIISKLIYPFNQEHPQFNLAKSQMKSGGSMISLKLNLNKEDTFKFCK
SLKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGIDDALVRLSIGCEDTNDLISDILFALNKF

Sequences:

>Translated_387_residues
MGNKENNIKKPGFKTLSIHHGETFAEETGCVMPPIFSTSTFKHGNKDNFDYTRSGNPNFKILENILKSIEDSKYCTVFGS
GISAVTAISSTLKSGDKILCESNLYGCTVRMFEKVFKKFGLEILYTDFTNENNVKKISNFEPTLIWLESPTNPLLKVLDI
KAICDEANKLEIPVVVDNTFSTALIQKPLDLGATLSVVSTTKFINGHSDALGGAVLTNNEEWNSKMLFSQKALGLQPSPF
DSWLITRGVKTLPLRIEQQTKSAEFISEELGNHKIISKLIYPFNQEHPQFNLAKSQMKSGGSMISLKLNLNKEDTFKFCK
SLKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGIDDALVRLSIGCEDTNDLISDILFALNKF
>Mature_386_residues
GNKENNIKKPGFKTLSIHHGETFAEETGCVMPPIFSTSTFKHGNKDNFDYTRSGNPNFKILENILKSIEDSKYCTVFGSG
ISAVTAISSTLKSGDKILCESNLYGCTVRMFEKVFKKFGLEILYTDFTNENNVKKISNFEPTLIWLESPTNPLLKVLDIK
AICDEANKLEIPVVVDNTFSTALIQKPLDLGATLSVVSTTKFINGHSDALGGAVLTNNEEWNSKMLFSQKALGLQPSPFD
SWLITRGVKTLPLRIEQQTKSAEFISEELGNHKIISKLIYPFNQEHPQFNLAKSQMKSGGSMISLKLNLNKEDTFKFCKS
LKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGIDDALVRLSIGCEDTNDLISDILFALNKF

Specific function: The enzymatic degradation of amino acids in cheese is believed to generate aroma compounds and therefore to be essential for flavor development. Cystathionine beta-lyase (CBL) can convert cystathionine to homocysteine but is also able to catalyze an alpha

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=362, Percent_Identity=40.8839779005525, Blast_Score=276, Evalue=2e-74,
Organism=Homo sapiens, GI299473759, Length=362, Percent_Identity=37.292817679558, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI25453487, Length=361, Percent_Identity=36.2880886426593, Blast_Score=224, Evalue=8e-59,
Organism=Escherichia coli, GI1790375, Length=370, Percent_Identity=35.4054054054054, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1789383, Length=354, Percent_Identity=25.9887005649718, Blast_Score=126, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538055, Length=368, Percent_Identity=42.6630434782609, Blast_Score=296, Evalue=9e-81,
Organism=Caenorhabditis elegans, GI17560056, Length=368, Percent_Identity=41.8478260869565, Blast_Score=293, Evalue=7e-80,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=32.6145552560647, Blast_Score=193, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6319307, Length=387, Percent_Identity=39.7932816537468, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6321254, Length=399, Percent_Identity=30.5764411027569, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6321904, Length=390, Percent_Identity=30.2564102564103, Blast_Score=136, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6323333, Length=429, Percent_Identity=29.3706293706294, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=318, Percent_Identity=28.9308176100629, Blast_Score=126, Evalue=5e-30,
Organism=Drosophila melanogaster, GI19922554, Length=384, Percent_Identity=39.84375, Blast_Score=288, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 42920; Mature: 42788

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNKENNIKKPGFKTLSIHHGETFAEETGCVMPPIFSTSTFKHGNKDNFDYTRSGNPNFK
CCCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ILENILKSIEDSKYCTVFGSGISAVTAISSTLKSGDKILCESNLYGCTVRMFEKVFKKFG
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCC
LEILYTDFTNENNVKKISNFEPTLIWLESPTNPLLKVLDIKAICDEANKLEIPVVVDNTF
CEEEEEECCCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCH
STALIQKPLDLGATLSVVSTTKFINGHSDALGGAVLTNNEEWNSKMLFSQKALGLQPSPF
HHHHHHCCCCCCCEEEEHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCC
DSWLITRGVKTLPLRIEQQTKSAEFISEELGNHKIISKLIYPFNQEHPQFNLAKSQMKSG
CHHHHHCCCEECCEEEECHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHCC
GSMISLKLNLNKEDTFKFCKSLKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGID
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCCCCCHHHHHCCCC
DALVRLSIGCEDTNDLISDILFALNKF
CEEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GNKENNIKKPGFKTLSIHHGETFAEETGCVMPPIFSTSTFKHGNKDNFDYTRSGNPNFK
CCCCCCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
ILENILKSIEDSKYCTVFGSGISAVTAISSTLKSGDKILCESNLYGCTVRMFEKVFKKFG
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHCC
LEILYTDFTNENNVKKISNFEPTLIWLESPTNPLLKVLDIKAICDEANKLEIPVVVDNTF
CEEEEEECCCCCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCH
STALIQKPLDLGATLSVVSTTKFINGHSDALGGAVLTNNEEWNSKMLFSQKALGLQPSPF
HHHHHHCCCCCCCEEEEHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCCC
DSWLITRGVKTLPLRIEQQTKSAEFISEELGNHKIISKLIYPFNQEHPQFNLAKSQMKSG
CHHHHHCCCEECCEEEECHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHCC
GSMISLKLNLNKEDTFKFCKSLKYFSLAESLGGVESLICHPATMTHASVDDKTKNLLGID
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCHHCCCCCCCHHHHHCCCC
DALVRLSIGCEDTNDLISDILFALNKF
CEEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]