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The map label for this gene is mdeA [H]

Identifier: 123967995

GI number: 123967995

Start: 398456

End: 399925

Strand: Reverse

Name: mdeA [H]

Synonym: A9601_04581

Alternate gene names: 123967995

Gene position: 399925-398456 (Counterclockwise)

Preceding gene: 123967996

Following gene: 123967994

Centisome position: 23.95

GC content: 29.66

Gene sequence:

>1470_bases
TTGAGAGATTTACTTAAAAAACCTATATGGAAAAATTTAGAGTTGGGATATGCAATTCCTGATAGTATTCATGCTGTTTC
TGTAGCATTACCAACTTGGAATGATGTAATAAATTACGAGGAAAAAGATCAAGAATGCATGAATTTATTGAAGTCCATTT
ACCCACGATTCGGGCTAAACCCCATAGTGAAAAGATTATGCGAAAAAGTAAAAAAACAAAATTACTACAACAATAAAAGT
ATCTGGCCATATCCGAATGAAAGAATAGCTTTTAAAGCTAAAAAATACATTGATAGAAATACTTCTGAACAATTCTCGTT
AATAGAAAAAAGAAATAATTTAGCTTTCTTAATAACTGAAAAAGAAGGAAGTATTTATGCAAAATATTTTTGGCAACATA
CTGGTCTTGGTCTATCTTCAAGAGCTGCCGCTATAGAACTAGGTCTTGAAGATTGCCCTCCAAAATCTTACGTAAATGAA
TGTTCTCAAAGAATAAAAAATAGAATTTCTAAATCTACAAAAATTGACTCTAATGATATTCACTTAACTTCATCTGGAAT
GTCTGCATTGCATACATCATTAGAAATCATATATAAATTATTTCCAGCTAAACCAACACTCCAAGTTGGTTTTCCATATG
TAGATGTACTTAAATTACCAATGAAAATCTTTCACGGAGCAAAGTTAATTACAGAAGAAAATTGCGCGGATATTGAATTA
GAAATCAAAAGAATAAATCCATCAGCATTAATTATTGAACTTCCAAGTAATCCAATGCTCAAATGTGTAAACATTAAAAA
AATTTCAAAAATTGCAAAAAAGCTAAATATTCCCTTAATTGTTGACGATACAATTGGTTCTAATTTAAATATAAATTCCA
TAGAACATGCAGATATAGTTTTTACTTCACTTACAAAAATTTTTTCAGGTAGTGGTGATATTCTTGCCGGATCATTAATA
CTAAATCCAAAAAGCAAATGGATTGATCAGTTAAGAAATGCATTGAACGAGATTAATATTCCAATACTTTCCGATGGAGA
TATAGTTTATCTTGAGAAAGTTAGTAGAGATGTAAAACAAAGAGTTTTTGAACAAAATAAAGCATGTTTAGAATTAAAAA
AAAGATTAGAGACTCATAGCGAGATTAAAAATATTTTCCATCCTGAAAATTGTCCAAATTTTAATTCTTTACTTACTTCT
AATGGGGGATACGGCTGCTTATTATCGTTTGAATTAAATGGAGGATTGAACAAAGCTAAAAAATTTTATGATTCTCTAAA
AGTATCTAAAGGACCTAGTTTAGGTACAAAATTTACTCTAGTTTGTCCTTATGTTTTACTAGCTCATTATGACGAGTTGG
ATTGGGCTGAAAGTTTTGGTATACCCTCGCACCTTATTAGAGTATCAGTTGGATTAGAAGACCAAGATCAATTATGGAAA
ACCTTTTCTGAAGCACTAAATAATTTCTAA

Upstream 100 bases:

>100_bases
ACTAGGGATAGATGATGCTCTTGTCAGATTATCAATTGGATGTGAAGATACAAACGATTTAATCTCGGACATTTTATTTG
CTTTAAATAAATTCTAAAAA

Downstream 100 bases:

>100_bases
ATTCCTAGTATTTACCGTATAGAAACTTATATACTCTTTTTCTGAATAATAGTTAGTATTAATATATATTTTCTCAATAT
ATAAATGGCAAAAATTTATG

Product: putative cystathionine gamma-synthase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MRDLLKKPIWKNLELGYAIPDSIHAVSVALPTWNDVINYEEKDQECMNLLKSIYPRFGLNPIVKRLCEKVKKQNYYNNKS
IWPYPNERIAFKAKKYIDRNTSEQFSLIEKRNNLAFLITEKEGSIYAKYFWQHTGLGLSSRAAAIELGLEDCPPKSYVNE
CSQRIKNRISKSTKIDSNDIHLTSSGMSALHTSLEIIYKLFPAKPTLQVGFPYVDVLKLPMKIFHGAKLITEENCADIEL
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKKLNIPLIVDDTIGSNLNINSIEHADIVFTSLTKIFSGSGDILAGSLI
LNPKSKWIDQLRNALNEINIPILSDGDIVYLEKVSRDVKQRVFEQNKACLELKKRLETHSEIKNIFHPENCPNFNSLLTS
NGGYGCLLSFELNGGLNKAKKFYDSLKVSKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPSHLIRVSVGLEDQDQLWK
TFSEALNNF

Sequences:

>Translated_489_residues
MRDLLKKPIWKNLELGYAIPDSIHAVSVALPTWNDVINYEEKDQECMNLLKSIYPRFGLNPIVKRLCEKVKKQNYYNNKS
IWPYPNERIAFKAKKYIDRNTSEQFSLIEKRNNLAFLITEKEGSIYAKYFWQHTGLGLSSRAAAIELGLEDCPPKSYVNE
CSQRIKNRISKSTKIDSNDIHLTSSGMSALHTSLEIIYKLFPAKPTLQVGFPYVDVLKLPMKIFHGAKLITEENCADIEL
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKKLNIPLIVDDTIGSNLNINSIEHADIVFTSLTKIFSGSGDILAGSLI
LNPKSKWIDQLRNALNEINIPILSDGDIVYLEKVSRDVKQRVFEQNKACLELKKRLETHSEIKNIFHPENCPNFNSLLTS
NGGYGCLLSFELNGGLNKAKKFYDSLKVSKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPSHLIRVSVGLEDQDQLWK
TFSEALNNF
>Mature_489_residues
MRDLLKKPIWKNLELGYAIPDSIHAVSVALPTWNDVINYEEKDQECMNLLKSIYPRFGLNPIVKRLCEKVKKQNYYNNKS
IWPYPNERIAFKAKKYIDRNTSEQFSLIEKRNNLAFLITEKEGSIYAKYFWQHTGLGLSSRAAAIELGLEDCPPKSYVNE
CSQRIKNRISKSTKIDSNDIHLTSSGMSALHTSLEIIYKLFPAKPTLQVGFPYVDVLKLPMKIFHGAKLITEENCADIEL
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKKLNIPLIVDDTIGSNLNINSIEHADIVFTSLTKIFSGSGDILAGSLI
LNPKSKWIDQLRNALNEINIPILSDGDIVYLEKVSRDVKQRVFEQNKACLELKKRLETHSEIKNIFHPENCPNFNSLLTS
NGGYGCLLSFELNGGLNKAKKFYDSLKVSKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPSHLIRVSVGLEDQDQLWK
TFSEALNNF

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1790375, Length=318, Percent_Identity=25.7861635220126, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17560056, Length=264, Percent_Identity=27.6515151515151, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17538055, Length=274, Percent_Identity=27.7372262773723, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322970, Length=566, Percent_Identity=31.8021201413428, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6322590, Length=349, Percent_Identity=34.0974212034384, Blast_Score=185, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6323557, Length=348, Percent_Identity=34.4827586206897, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6319307, Length=250, Percent_Identity=30, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19922554, Length=244, Percent_Identity=29.5081967213115, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 55458; Mature: 55458

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLLKKPIWKNLELGYAIPDSIHAVSVALPTWNDVINYEEKDQECMNLLKSIYPRFGLN
CCHHHHCCCCCCCCCCEECCCCCCEEEEECCCCHHHCCCCHHHHHHHHHHHHHCCCCCCC
PIVKRLCEKVKKQNYYNNKSIWPYPNERIAFKAKKYIDRNTSEQFSLIEKRNNLAFLITE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHCCCCHHHHHHHHCCCCEEEEEEE
KEGSIYAKYFWQHTGLGLSSRAAAIELGLEDCPPKSYVNECSQRIKNRISKSTKIDSNDI
CCCCEEEEEEHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCE
HLTSSGMSALHTSLEIIYKLFPAKPTLQVGFPYVDVLKLPMKIFHGAKLITEENCADIEL
EEECCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCEEECCCCCCEEE
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKKLNIPLIVDDTIGSNLNINSIEHADIV
EEEEECCCEEEEECCCCCCEEEECHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHH
FTSLTKIFSGSGDILAGSLILNPKSKWIDQLRNALNEINIPILSDGDIVYLEKVSRDVKQ
HHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCEEECCCCEEEHHHHHHHHHH
RVFEQNKACLELKKRLETHSEIKNIFHPENCPNFNSLLTSNGGYGCLLSFELNGGLNKAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEEEEECCCHHHHH
KFYDSLKVSKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPSHLIRVSVGLEDQDQLWK
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCHHEEEEEECCCCHHHHHH
TFSEALNNF
HHHHHHHCC
>Mature Secondary Structure
MRDLLKKPIWKNLELGYAIPDSIHAVSVALPTWNDVINYEEKDQECMNLLKSIYPRFGLN
CCHHHHCCCCCCCCCCEECCCCCCEEEEECCCCHHHCCCCHHHHHHHHHHHHHCCCCCCC
PIVKRLCEKVKKQNYYNNKSIWPYPNERIAFKAKKYIDRNTSEQFSLIEKRNNLAFLITE
HHHHHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHCCCCHHHHHHHHCCCCEEEEEEE
KEGSIYAKYFWQHTGLGLSSRAAAIELGLEDCPPKSYVNECSQRIKNRISKSTKIDSNDI
CCCCEEEEEEHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCE
HLTSSGMSALHTSLEIIYKLFPAKPTLQVGFPYVDVLKLPMKIFHGAKLITEENCADIEL
EEECCCHHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHCCCCEEECCCCCCEEE
EIKRINPSALIIELPSNPMLKCVNIKKISKIAKKLNIPLIVDDTIGSNLNINSIEHADIV
EEEEECCCEEEEECCCCCCEEEECHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHH
FTSLTKIFSGSGDILAGSLILNPKSKWIDQLRNALNEINIPILSDGDIVYLEKVSRDVKQ
HHHHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHHCCCEEECCCCEEEHHHHHHHHHH
RVFEQNKACLELKKRLETHSEIKNIFHPENCPNFNSLLTSNGGYGCLLSFELNGGLNKAK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCEEEEEEEEECCCHHHHH
KFYDSLKVSKGPSLGTKFTLVCPYVLLAHYDELDWAESFGIPSHLIRVSVGLEDQDQLWK
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHCCCCHHEEEEEECCCCHHHHHH
TFSEALNNF
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]