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The map label for this gene is gcvA [H]

Identifier: 121610129

GI number: 121610129

Start: 3554996

End: 3555910

Strand: Direct

Name: gcvA [H]

Synonym: Veis_3188

Alternate gene names: 121610129

Gene position: 3554996-3555910 (Clockwise)

Preceding gene: 121610128

Following gene: 121610130

Centisome position: 63.86

GC content: 61.75

Gene sequence:

>915_bases
ATGCACCTGCCTTCCATTACCGCGTTAAGGGCGTTGGATGCCATCGCCAGGCATGGCACCGTGTCGCGCGCAGCCAGCGA
ACTTTTTCTGACACGCAGCGCAATCAGTCACCAAATCAACGTGCTGGAACAAACCCTTGGCTTTGCAGTAACGCAAAGAG
CAGGGCGCGGTATCACGCTGACCTATCGGGGTCAGCGCTATCTGCACGAGGCCCGGCGTGTGCTTTCCATCCTGGAAGAG
GCGGTGCGGCGTAGCGCCGGTGGCGAAGTGGCCGGACATTTGCGTGTGAGCTGCACGCCTGGTTTTGCGGTCTACTGGCT
CTGTCACCATCTCGGTGCGTTTCAGGGCGCGTACCCGCATGTGGAGCTGAGCATCACAGCGCCGCGTGCGTCGGACGAAG
TGAACCCGCGCGACGCCGACCTGTTTATCGTCTATGGCAGTGGCAACTGGCCTGACTTTTCTGTTGAACCCATCACCTCG
CTGAGTTACTTTCCCGTCTGCAGTCCGATGCTGATCAATGCACGCGGCCGTCCGCTGGCGCTGGATGATCTCGCCGATGT
ACTGCTGTTGCACATGGGCAATCATGCGGACTGGGCGCAGTGGCTCGGGGCTGCGGGTGCGCACCACATCGACGGCCGGC
GTGGCATCGTGTTTTCGGAAGCTCCGTTCGCGCAGGAAGCAGCGATCTCGGGGCAGGGCGTGACGATCGGCGATACCTTC
CTGAGCGGTGATGCGATCGCTCGCGGGCTACTCGTGCGCCCGTTCGATGTGACGGTTGAATCGCCGCACGGCTACTACCT
GGTGGCGCGTCCGGAGCCGTCGCAACGTCCGGAAGTTCGCGCCTTCAGTGAATGGTTGCTCGATGAGTTGCGCGTGTCGA
TACGCGCATGGAGCGATCACTCGCGTTACGCATGA

Upstream 100 bases:

>100_bases
CCCCCTCTGCACCGAGGTGGTTGATAAACTGCTTGGGCACGATGCGATCATGGCGCCAGAAAGCACCATTGCAACAGACC
CTCCGTCGCTGACAGACCCC

Downstream 100 bases:

>100_bases
AGTGAAAAGCGGCGCTGCCTGATGGCGTGCCAAGGCGCGCCCACGGCATGTTTTTGGCAGTTCGTTGGCTCGCTCCATCC
GGAAAACCACCGCCGACCTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MHLPSITALRALDAIARHGTVSRAASELFLTRSAISHQINVLEQTLGFAVTQRAGRGITLTYRGQRYLHEARRVLSILEE
AVRRSAGGEVAGHLRVSCTPGFAVYWLCHHLGAFQGAYPHVELSITAPRASDEVNPRDADLFIVYGSGNWPDFSVEPITS
LSYFPVCSPMLINARGRPLALDDLADVLLLHMGNHADWAQWLGAAGAHHIDGRRGIVFSEAPFAQEAAISGQGVTIGDTF
LSGDAIARGLLVRPFDVTVESPHGYYLVARPEPSQRPEVRAFSEWLLDELRVSIRAWSDHSRYA

Sequences:

>Translated_304_residues
MHLPSITALRALDAIARHGTVSRAASELFLTRSAISHQINVLEQTLGFAVTQRAGRGITLTYRGQRYLHEARRVLSILEE
AVRRSAGGEVAGHLRVSCTPGFAVYWLCHHLGAFQGAYPHVELSITAPRASDEVNPRDADLFIVYGSGNWPDFSVEPITS
LSYFPVCSPMLINARGRPLALDDLADVLLLHMGNHADWAQWLGAAGAHHIDGRRGIVFSEAPFAQEAAISGQGVTIGDTF
LSGDAIARGLLVRPFDVTVESPHGYYLVARPEPSQRPEVRAFSEWLLDELRVSIRAWSDHSRYA
>Mature_304_residues
MHLPSITALRALDAIARHGTVSRAASELFLTRSAISHQINVLEQTLGFAVTQRAGRGITLTYRGQRYLHEARRVLSILEE
AVRRSAGGEVAGHLRVSCTPGFAVYWLCHHLGAFQGAYPHVELSITAPRASDEVNPRDADLFIVYGSGNWPDFSVEPITS
LSYFPVCSPMLINARGRPLALDDLADVLLLHMGNHADWAQWLGAAGAHHIDGRRGIVFSEAPFAQEAAISGQGVTIGDTF
LSGDAIARGLLVRPFDVTVESPHGYYLVARPEPSQRPEVRAFSEWLLDELRVSIRAWSDHSRYA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=286, Percent_Identity=37.7622377622378, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=31.9587628865979, Blast_Score=118, Evalue=5e-28,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=28.956228956229, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI1786401, Length=291, Percent_Identity=25.4295532646048, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI145693193, Length=300, Percent_Identity=25, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI157672245, Length=183, Percent_Identity=28.9617486338798, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI87081978, Length=259, Percent_Identity=26.6409266409266, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33353; Mature: 33353

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLPSITALRALDAIARHGTVSRAASELFLTRSAISHQINVLEQTLGFAVTQRAGRGITL
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
TYRGQRYLHEARRVLSILEEAVRRSAGGEVAGHLRVSCTPGFAVYWLCHHLGAFQGAYPH
EECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCE
VELSITAPRASDEVNPRDADLFIVYGSGNWPDFSVEPITSLSYFPVCSPMLINARGRPLA
EEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEE
LDDLADVLLLHMGNHADWAQWLGAAGAHHIDGRRGIVFSEAPFAQEAAISGQGVTIGDTF
HHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHCCCCCEEEEHHH
LSGDAIARGLLVRPFDVTVESPHGYYLVARPEPSQRPEVRAFSEWLLDELRVSIRAWSDH
HCCHHHHCCEEECCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEECCC
SRYA
CCCC
>Mature Secondary Structure
MHLPSITALRALDAIARHGTVSRAASELFLTRSAISHQINVLEQTLGFAVTQRAGRGITL
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
TYRGQRYLHEARRVLSILEEAVRRSAGGEVAGHLRVSCTPGFAVYWLCHHLGAFQGAYPH
EECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCE
VELSITAPRASDEVNPRDADLFIVYGSGNWPDFSVEPITSLSYFPVCSPMLINARGRPLA
EEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCEE
LDDLADVLLLHMGNHADWAQWLGAAGAHHIDGRRGIVFSEAPFAQEAAISGQGVTIGDTF
HHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHCCCCCEEEEHHH
LSGDAIARGLLVRPFDVTVESPHGYYLVARPEPSQRPEVRAFSEWLLDELRVSIRAWSDH
HCCHHHHCCEEECCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEECCC
SRYA
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]