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The map label for this gene is fadE32 [H]

Identifier: 121639482

GI number: 121639482

Start: 3983183

End: 3984142

Strand: Direct

Name: fadE32 [H]

Synonym: BCG_3627

Alternate gene names: 121639482

Gene position: 3983183-3984142 (Clockwise)

Preceding gene: 121639481

Following gene: 121639483

Centisome position: 91.05

GC content: 69.79

Gene sequence:

>960_bases
GTGACCATGGAATTCGCACTCAACGAACAGCAGCGCGACTTCGCGGCCAGCATCGACGCGGCGCTCGGCGCCGCCGACCT
GCCCGGCGTCGTCCGTGCTTGGGCTGCCGGTGATGTGGCGCCCGGCCGCAAGGTGTGGCAGCAGTTGGCCAACCTGGGCG
TCACCGCGTTGGGCGTAGCGGAGAAGTTCGACGGACTGGGTGCCAGTCCGGTCGATCTGGTTGTCGCGCTCGAACGTCTC
GGGCGCTGGTGCGTGCCCGGCCCGGTCACCGAATCCATTGCCGTGGCACCGATTCTGCTGGCTCATGATGATCGGGCTGA
ACGCAGCCATGGGCTAGCTTCCGGTGAGCTCATCGCCACCGTGGCCATGCCGCCGCGGGTTCCGCGCGCCGTCGACGCCG
ACACCGCCGGGCTGGTACTGCTCGCGGGCGATGGCAGCGTCACCGAAGGGACGCCGGGTGATTGCCACCGGTCCGTCGAC
CCCAGCCGGCGGCTGTATGAGGTGGCGGCATCCGGCCAGGCCTGGCGGGCCCCGAAAGACGTAGTGGCGCGCGCCTATGA
GTTCGGGGCGCTGGCCACCGCCGCACAACTGGTCGGCGCCGGGCAGGCGCTGCTGGAGGCCGCCGTCAACTACGCCAAAC
AGCGCACGCAGTTCGGCCGGGCGATCGGCTCGTATCAGGCCATCAAGCACAAACTCGCCGACGTGCACATTGCGATCGAG
CTGGCCTGCCCCCTGGTTTACGGCGCGGCCGTGTCACTCGAGCCGCGCGATGTCAGCGCCGCCAAAGCCGCCGCGAGCGA
GGCGGCTCTGCTGGCGGCACGCTCGGCGTTGCAGACCCACGGCGCCATCGGGTTCACCTGCGAGCATGACCTGTCGCTGT
GGTTGTTGCGGGTGCAGGCGTTGCACTCGGCCTGGGGTACGCCGCAGGAGCATCGGCGGCGTGTGCTGGAGGCGCTATGA

Upstream 100 bases:

>100_bases
GGTTGCTGTTCGCCCTGGGCGGCCCGATCTATGCCGGGACCAACGAAATCCAGCGCAACATCATTGCCGAACGGCTGCTG
GGCCTGCCACGCGAGAAGAC

Downstream 100 bases:

>100_bases
CCCCCCCTGAAGAACGGCAGATGCTACGGGAAACCGTCGCCTCCCTGGTGGCTAAGCATGCCGGCCCGGCGGCGGTGCGC
GCAGCGATGGCCTCCGACCG

Product: putative acyl-CoA dehydrogenase fadE32

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTMEFALNEQQRDFAASIDAALGAADLPGVVRAWAAGDVAPGRKVWQQLANLGVTALGVAEKFDGLGASPVDLVVALERL
GRWCVPGPVTESIAVAPILLAHDDRAERSHGLASGELIATVAMPPRVPRAVDADTAGLVLLAGDGSVTEGTPGDCHRSVD
PSRRLYEVAASGQAWRAPKDVVARAYEFGALATAAQLVGAGQALLEAAVNYAKQRTQFGRAIGSYQAIKHKLADVHIAIE
LACPLVYGAAVSLEPRDVSAAKAAASEAALLAARSALQTHGAIGFTCEHDLSLWLLRVQALHSAWGTPQEHRRRVLEAL

Sequences:

>Translated_319_residues
MTMEFALNEQQRDFAASIDAALGAADLPGVVRAWAAGDVAPGRKVWQQLANLGVTALGVAEKFDGLGASPVDLVVALERL
GRWCVPGPVTESIAVAPILLAHDDRAERSHGLASGELIATVAMPPRVPRAVDADTAGLVLLAGDGSVTEGTPGDCHRSVD
PSRRLYEVAASGQAWRAPKDVVARAYEFGALATAAQLVGAGQALLEAAVNYAKQRTQFGRAIGSYQAIKHKLADVHIAIE
LACPLVYGAAVSLEPRDVSAAKAAASEAALLAARSALQTHGAIGFTCEHDLSLWLLRVQALHSAWGTPQEHRRRVLEAL
>Mature_318_residues
TMEFALNEQQRDFAASIDAALGAADLPGVVRAWAAGDVAPGRKVWQQLANLGVTALGVAEKFDGLGASPVDLVVALERLG
RWCVPGPVTESIAVAPILLAHDDRAERSHGLASGELIATVAMPPRVPRAVDADTAGLVLLAGDGSVTEGTPGDCHRSVDP
SRRLYEVAASGQAWRAPKDVVARAYEFGALATAAQLVGAGQALLEAAVNYAKQRTQFGRAIGSYQAIKHKLADVHIAIEL
ACPLVYGAAVSLEPRDVSAAKAAASEAALLAARSALQTHGAIGFTCEHDLSLWLLRVQALHSAWGTPQEHRRRVLEAL

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Drosophila melanogaster, GI21356377, Length=105, Percent_Identity=39.0476190476191, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 33416; Mature: 33285

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00464 RIBOSOMAL_L22

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMEFALNEQQRDFAASIDAALGAADLPGVVRAWAAGDVAPGRKVWQQLANLGVTALGVA
CCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHH
EKFDGLGASPVDLVVALERLGRWCVPGPVTESIAVAPILLAHDDRAERSHGLASGELIAT
HHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHEEEEECCCHHHHHCCCCCCCEEEE
VAMPPRVPRAVDADTAGLVLLAGDGSVTEGTPGDCHRSVDPSRRLYEVAASGQAWRAPKD
EECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHH
VVARAYEFGALATAAQLVGAGQALLEAAVNYAKQRTQFGRAIGSYQAIKHKLADVHIAIE
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
LACPLVYGAAVSLEPRDVSAAKAAASEAALLAARSALQTHGAIGFTCEHDLSLWLLRVQA
EHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
LHSAWGTPQEHRRRVLEAL
HHHHCCCCHHHHHHHHHHC
>Mature Secondary Structure 
TMEFALNEQQRDFAASIDAALGAADLPGVVRAWAAGDVAPGRKVWQQLANLGVTALGVA
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHH
EKFDGLGASPVDLVVALERLGRWCVPGPVTESIAVAPILLAHDDRAERSHGLASGELIAT
HHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHEEEEECCCHHHHHCCCCCCCEEEE
VAMPPRVPRAVDADTAGLVLLAGDGSVTEGTPGDCHRSVDPSRRLYEVAASGQAWRAPKD
EECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHH
VVARAYEFGALATAAQLVGAGQALLEAAVNYAKQRTQFGRAIGSYQAIKHKLADVHIAIE
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
LACPLVYGAAVSLEPRDVSAAKAAASEAALLAARSALQTHGAIGFTCEHDLSLWLLRVQA
EHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHH
LHSAWGTPQEHRRRVLEAL
HHHHCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]