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The map label for this gene is fadE31 [H]

Identifier: 121639481

GI number: 121639481

Start: 3982053

End: 3983186

Strand: Direct

Name: fadE31 [H]

Synonym: BCG_3626

Alternate gene names: 121639481

Gene position: 3982053-3983186 (Clockwise)

Preceding gene: 121639480

Following gene: 121639482

Centisome position: 91.03

GC content: 66.93

Gene sequence:

>1134_bases
ATGGACCTGAATTTCGACGACGAGACCCTGGCCTTTCAGGCCGAGGTGCGCGAGTTCCTCGCCGCCAATGCCGCATCGAT
CCCGACGAAGTCCTACGACAATGCGGAAGGCTTTGCGCAACACCGTTATTGGGACCGAGTACTGTTCGACGCGGGCCTGT
CGGTGATCACCTGGCCGGCTAAGTATGGTGGCCGGGACGCGCCGCTGCTGCACTGGATCGTGTTCGAGGAGGAGTACTTT
CGCGCCGGCGCCCCGGGCCGGGCCAGCGCCAACGGCACCTCGATGCTGGCGCCGACGCTGTTCGCGCACGGCACAGCCGA
ACAGCTTGACCGGATCCTGCCGAAAATGGCTAGCGGCGAACAGATCTGGGCGCAGGCCTGGTCGGAGCCGGAATCCGGCA
GCGACCTGGCGTCGCTGCGCTCCACCGCGAGCAAGGTCGACGGCGGCTGGCTACTCAACGGGCAGAAGATCTGGAGCTCG
CGGGCGCCGTTCGCCGACATGGGTTTTGGGCTGTTCCGCTCCGATCCCGCGGTCGAACGGCACCGCGGGCTCACGTATTT
CATGTTCGACCTGAAAGCCAAGGGTGTTACCGTGCGCCCAATCGCCCAACTGGGCGGCGACACCGGTTTCGGTGAGATCT
TTCTCGACGACGTGTTCGTCCCCGACCGGGATGTGATTGGGGCACCGAACGACGGATGGCGCGCGGCCATGAGCACGTCA
AGCAACGAGCGCGGCATGTCGCTGCGCAGCCCAGCCCGCTTCCTGGCCTCCGCCGAACGGCTGGTCCAGCTGTGGAAGGA
CCGCGGCTCGCCCCCGGAGTTCGCCGACCGGGTCGCCGACGCCTGGATCAAGGCGCAGGCCTACCGGCTGCAGACCTTCG
GCACGGTGACCAGGCTGGCCGCCGGTGGCGAACTGGGGGCGGAATCGTCGGTGACCAAGGTGTTCTGGTCCGAGCTGGAC
GTGCACTTGCATCAGACCGCGCTCGACCTGCGCGGCGCCGATGGGGAGCTGGCCGGCCCGTGGACCGAGGGGTTGCTGTT
CGCCCTGGGCGGCCCGATCTATGCCGGGACCAACGAAATCCAGCGCAACATCATTGCCGAACGGCTGCTGGGCCTGCCAC
GCGAGAAGACGTGA

Upstream 100 bases:

>100_bases
GGCGAACTTCAAGACACCCCGGTCGGTGCGGTTCGTCGACGTACTGCCGCGCAACGCCGCCGGTAAGGTGAGCAAACCAC
AACTGCGAGAGCTGGGCTAG

Downstream 100 bases:

>100_bases
CCATGGAATTCGCACTCAACGAACAGCAGCGCGACTTCGCGGCCAGCATCGACGCGGCGCTCGGCGCCGCCGACCTGCCC
GGCGTCGTCCGTGCTTGGGC

Product: putative acyl-CoA dehydrogenase fadE31

Products: gamma-butyrobetaine; Acceptor; gamma-butyrobetainyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MDLNFDDETLAFQAEVREFLAANAASIPTKSYDNAEGFAQHRYWDRVLFDAGLSVITWPAKYGGRDAPLLHWIVFEEEYF
RAGAPGRASANGTSMLAPTLFAHGTAEQLDRILPKMASGEQIWAQAWSEPESGSDLASLRSTASKVDGGWLLNGQKIWSS
RAPFADMGFGLFRSDPAVERHRGLTYFMFDLKAKGVTVRPIAQLGGDTGFGEIFLDDVFVPDRDVIGAPNDGWRAAMSTS
SNERGMSLRSPARFLASAERLVQLWKDRGSPPEFADRVADAWIKAQAYRLQTFGTVTRLAAGGELGAESSVTKVFWSELD
VHLHQTALDLRGADGELAGPWTEGLLFALGGPIYAGTNEIQRNIIAERLLGLPREKT

Sequences:

>Translated_377_residues
MDLNFDDETLAFQAEVREFLAANAASIPTKSYDNAEGFAQHRYWDRVLFDAGLSVITWPAKYGGRDAPLLHWIVFEEEYF
RAGAPGRASANGTSMLAPTLFAHGTAEQLDRILPKMASGEQIWAQAWSEPESGSDLASLRSTASKVDGGWLLNGQKIWSS
RAPFADMGFGLFRSDPAVERHRGLTYFMFDLKAKGVTVRPIAQLGGDTGFGEIFLDDVFVPDRDVIGAPNDGWRAAMSTS
SNERGMSLRSPARFLASAERLVQLWKDRGSPPEFADRVADAWIKAQAYRLQTFGTVTRLAAGGELGAESSVTKVFWSELD
VHLHQTALDLRGADGELAGPWTEGLLFALGGPIYAGTNEIQRNIIAERLLGLPREKT
>Mature_377_residues
MDLNFDDETLAFQAEVREFLAANAASIPTKSYDNAEGFAQHRYWDRVLFDAGLSVITWPAKYGGRDAPLLHWIVFEEEYF
RAGAPGRASANGTSMLAPTLFAHGTAEQLDRILPKMASGEQIWAQAWSEPESGSDLASLRSTASKVDGGWLLNGQKIWSS
RAPFADMGFGLFRSDPAVERHRGLTYFMFDLKAKGVTVRPIAQLGGDTGFGEIFLDDVFVPDRDVIGAPNDGWRAAMSTS
SNERGMSLRSPARFLASAERLVQLWKDRGSPPEFADRVADAWIKAQAYRLQTFGTVTRLAAGGELGAESSVTKVFWSELD
VHLHQTALDLRGADGELAGPWTEGLLFALGGPIYAGTNEIQRNIIAERLLGLPREKT

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI226958412, Length=364, Percent_Identity=27.4725274725275, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI226958414, Length=340, Percent_Identity=27.3529411764706, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI4501859, Length=379, Percent_Identity=25.5936675461741, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI4501857, Length=392, Percent_Identity=27.0408163265306, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI76496475, Length=289, Percent_Identity=28.719723183391, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI4557235, Length=289, Percent_Identity=28.719723183391, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI21361497, Length=288, Percent_Identity=29.5138888888889, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI4557233, Length=333, Percent_Identity=26.7267267267267, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI4557231, Length=383, Percent_Identity=22.976501305483, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI187960098, Length=383, Percent_Identity=22.976501305483, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1786223, Length=353, Percent_Identity=23.2294617563739, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87081958, Length=247, Percent_Identity=25.9109311740891, Blast_Score=81, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538396, Length=385, Percent_Identity=26.2337662337662, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17570075, Length=391, Percent_Identity=26.8542199488491, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508101, Length=334, Percent_Identity=24.5508982035928, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17569725, Length=338, Percent_Identity=25.4437869822485, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17534899, Length=329, Percent_Identity=25.531914893617, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17506239, Length=338, Percent_Identity=23.3727810650888, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71985192, Length=241, Percent_Identity=27.3858921161826, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71985184, Length=154, Percent_Identity=32.4675324675325, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71990804, Length=193, Percent_Identity=27.4611398963731, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17551932, Length=209, Percent_Identity=25.3588516746411, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71982178, Length=199, Percent_Identity=28.1407035175879, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI21355753, Length=354, Percent_Identity=25.9887005649718, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281363737, Length=287, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24646207, Length=373, Percent_Identity=26.2734584450402, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24666513, Length=336, Percent_Identity=25.5952380952381, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356377, Length=373, Percent_Identity=25.201072386059, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24660351, Length=327, Percent_Identity=25.9938837920489, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.- [C]

Molecular weight: Translated: 41333; Mature: 41333

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLNFDDETLAFQAEVREFLAANAASIPTKSYDNAEGFAQHRYWDRVLFDAGLSVITWPA
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECH
KYGGRDAPLLHWIVFEEEYFRAGAPGRASANGTSMLAPTLFAHGTAEQLDRILPKMASGE
HHCCCCCCEEEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCH
QIWAQAWSEPESGSDLASLRSTASKVDGGWLLNGQKIWSSRAPFADMGFGLFRSDPAVER
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHCCCCCHHHHCCCHHCCCCHHHH
HRGLTYFMFDLKAKGVTVRPIAQLGGDTGFGEIFLDDVFVPDRDVIGAPNDGWRAAMSTS
HCCCEEEEEEEECCCEEEEHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCC
SNERGMSLRSPARFLASAERLVQLWKDRGSPPEFADRVADAWIKAQAYRLQTFGTVTRLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGGELGAESSVTKVFWSELDVHLHQTALDLRGADGELAGPWTEGLLFALGGPIYAGTNEI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
QRNIIAERLLGLPREKT
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDLNFDDETLAFQAEVREFLAANAASIPTKSYDNAEGFAQHRYWDRVLFDAGLSVITWPA
CCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECH
KYGGRDAPLLHWIVFEEEYFRAGAPGRASANGTSMLAPTLFAHGTAEQLDRILPKMASGE
HHCCCCCCEEEEEEEEHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCH
QIWAQAWSEPESGSDLASLRSTASKVDGGWLLNGQKIWSSRAPFADMGFGLFRSDPAVER
HHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEECCHHHHCCCCCHHHHCCCHHCCCCHHHH
HRGLTYFMFDLKAKGVTVRPIAQLGGDTGFGEIFLDDVFVPDRDVIGAPNDGWRAAMSTS
HCCCEEEEEEEECCCEEEEHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCC
SNERGMSLRSPARFLASAERLVQLWKDRGSPPEFADRVADAWIKAQAYRLQTFGTVTRLA
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGGELGAESSVTKVFWSELDVHLHQTALDLRGADGELAGPWTEGLLFALGGPIYAGTNEI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
QRNIIAERLLGLPREKT
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: crotono-betaine; reduced acceptor; crotonobetainyl-CoA [C]

Specific reaction: reduced acceptor + crotono-betaine = Acceptor + gamma-butyrobetaine reduced acceptor + crotonobetainyl-CoA = Acceptor + gamma-butyrobetainyl-CoA [C]

General reaction: Oxidoreductases; Acting on the CH-CH group of donors; With other acceptors [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759838; 8969508; 9384377 [H]