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The map label for this gene is kshA

Identifier: 121639445

GI number: 121639445

Start: 3941331

End: 3942491

Strand: Direct

Name: kshA

Synonym: BCG_3590

Alternate gene names: 121639445

Gene position: 3941331-3942491 (Clockwise)

Preceding gene: 121639443

Following gene: 121639446

Centisome position: 90.1

GC content: 60.72

Gene sequence:

>1161_bases
GTGAGTACCGACACGAGTGGGGTCGGTGTTCGGGAGATCGATGCCGGCGCCTTGCCGACCAGGTATGCGCGTGGCTGGCA
TTGCCTGGGCGTCGCGAAGGACTATTTGGAAGGGAAGCCACACGGGGTAGAGGCGTTCGGCACCAAGCTGGTTGTGTTCG
CTGATTCCCACGGGGACCTGAAAGTCCTCGACGGCTACTGCCGGCACATGGGCGGCGACCTGTCCGAGGGCACCGTCAAA
GGCGACGAGGTCGCTTGCCCGTTCCACGACTGGCGCTGGGGTGGCGACGGCCGCTGCAAATTGGTGCCGTATGCCAGGCG
CACACCCAGAATGGCGCGCACTCGGTCGTGGACGACCGATGTGCGCAGCGGGCTGCTGTTTGTCTGGCACGACCATGAGG
GCAATCCACCCGACCCCGCGGTCCGGATCCCCGAGATTCCCGAGGCGGCCAGCGACGAGTGGACCGACTGGCGGTGGAAC
CGCATCCTCATCGAAGGGTCCAACTGCCGCGACATCATCGACAACGTCACCGATATGGCGCACTTCTTCTACATCCACTT
CGGTTTGCCGACGTACTTCAAGAACGTCTTCGAGGGCCACATCGCCTCGCAGTATCTGCACAACGTGGGCCGGCCCGATG
TCGACGATCTGGGGACGTCTTACGGTGAGGCGCATCTGGATTCGGAGGCGTCTTACTTCGGGCCGTCGTTCATGATCAAC
TGGCTGCACAACCGCTACGGCAACTACAAGTCCGAGTCGATCCTGATCAACTGCCACTACCCGGTGACCCAGAACTCGTT
CGTCCTGCAATGGGGCGTCATCGTCGAAAAGCCCAAGGGTATGAGCGAAGAGATGACCGACAAGTTGTCGCGGGTGTTCA
CCGAGGGCGTCAGCAAGGGCTTCTTGCAGGATGTCGAGATCTGGAAGCACAAGACCCGCATCGACAACCCGCTGCTGGTT
GAAGAGGACGGCGCCGTGTATCAGCTGCGCCGCTGGTATGAACAGTTTTATGTCGACGTAGCCGACATAAAACCAGAGAT
GGTGGAGCGCTTCGAGATCGAGGTCGACACCAAGCGCGCCAACGAGTTCTGGAATGCCGAGGTAGAGAAGAACTTGAAAT
CGAGAGAAGTTTCCGACGACGTGCCCGCCGAGCAACACTGA

Upstream 100 bases:

>100_bases
CCCGTCGTACATATGCCGTGGCCAGACTCGCTGTCGTTGCGCTCACCGGAGAGAAAACTGTAACGTGTTCTAGTTAGCGA
TACCGATCGGGAGGTGACAG

Downstream 100 bases:

>100_bases
CGGACATGCCTGACGATCAGCCGGCGGTTCCCGACGTCGATCGGCTGGCCCGGTCGATGCTACTGCTGCACGGTGATCAT
CACGATCACAACGATTCCCC

Product: putative oxidoreductase

Products: NA

Alternate protein names: Rieske-type oxygenase; RO

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSTDTSGVGVREIDAGALPTRYARGWHCLGVAKDYLEGKPHGVEAFGTKLVVFADSHGDLKVLDGYCRHMGGDLSEGTVK
GDEVACPFHDWRWGGDGRCKLVPYARRTPRMARTRSWTTDVRSGLLFVWHDHEGNPPDPAVRIPEIPEAASDEWTDWRWN
RILIEGSNCRDIIDNVTDMAHFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDVDDLGTSYGEAHLDSEASYFGPSFMIN
WLHNRYGNYKSESILINCHYPVTQNSFVLQWGVIVEKPKGMSEEMTDKLSRVFTEGVSKGFLQDVEIWKHKTRIDNPLLV
EEDGAVYQLRRWYEQFYVDVADIKPEMVERFEIEVDTKRANEFWNAEVEKNLKSREVSDDVPAEQH

Sequences:

>Translated_386_residues
MSTDTSGVGVREIDAGALPTRYARGWHCLGVAKDYLEGKPHGVEAFGTKLVVFADSHGDLKVLDGYCRHMGGDLSEGTVK
GDEVACPFHDWRWGGDGRCKLVPYARRTPRMARTRSWTTDVRSGLLFVWHDHEGNPPDPAVRIPEIPEAASDEWTDWRWN
RILIEGSNCRDIIDNVTDMAHFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDVDDLGTSYGEAHLDSEASYFGPSFMIN
WLHNRYGNYKSESILINCHYPVTQNSFVLQWGVIVEKPKGMSEEMTDKLSRVFTEGVSKGFLQDVEIWKHKTRIDNPLLV
EEDGAVYQLRRWYEQFYVDVADIKPEMVERFEIEVDTKRANEFWNAEVEKNLKSREVSDDVPAEQH
>Mature_385_residues
STDTSGVGVREIDAGALPTRYARGWHCLGVAKDYLEGKPHGVEAFGTKLVVFADSHGDLKVLDGYCRHMGGDLSEGTVKG
DEVACPFHDWRWGGDGRCKLVPYARRTPRMARTRSWTTDVRSGLLFVWHDHEGNPPDPAVRIPEIPEAASDEWTDWRWNR
ILIEGSNCRDIIDNVTDMAHFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDVDDLGTSYGEAHLDSEASYFGPSFMINW
LHNRYGNYKSESILINCHYPVTQNSFVLQWGVIVEKPKGMSEEMTDKLSRVFTEGVSKGFLQDVEIWKHKTRIDNPLLVE
EDGAVYQLRRWYEQFYVDVADIKPEMVERFEIEVDTKRANEFWNAEVEKNLKSREVSDDVPAEQH

Specific function: Catalyzes the opening of ring B of 1,4-androstadiene- 3,17,-dione (ADD) and 4-androstene-3,17-dione (ADD) with concomitant aromatization of ring A. The ring B is subsequently hydroxylated to yield a catechol and then subject to meta- cleavage

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain

Homologues:

Organism=Caenorhabditis elegans, GI71983709, Length=365, Percent_Identity=23.5616438356164, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI161085896, Length=355, Percent_Identity=27.6056338028169, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KSHA_MYCTU (P71875)

Other databases:

- EMBL:   BX842583
- PIR:   G70674
- RefSeq:   NP_218043.1
- PDB:   2ZYL
- PDBsum:   2ZYL
- ProteinModelPortal:   P71875
- EnsemblBacteria:   EBMYCT00000003502
- GeneID:   888268
- GenomeReviews:   AL123456_GR
- KEGG:   mtu:Rv3526
- TubercuList:   Rv3526
- GeneTree:   EBGT00050000015751
- HOGENOM:   HBG369067
- OMA:   GSNCREI
- ProtClustDB:   CLSK872190
- InterPro:   IPR017941
- Gene3D:   G3DSA:2.102.10.10

Pfam domain/function: PF00355 Rieske; SSF50022 Rieske_dom

EC number: NA

Molecular weight: Translated: 44268; Mature: 44137

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS51296 RIESKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDTSGVGVREIDAGALPTRYARGWHCLGVAKDYLEGKPHGVEAFGTKLVVFADSHGDL
CCCCCCCCCEEECCCCCCCHHHCCCEEEEHHHHHHHCCCCCCCCCCCCEEEEEECCCCCC
KVLDGYCRHMGGDLSEGTVKGDEVACPFHDWRWGGDGRCKLVPYARRTPRMARTRSWTTD
HHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCHHCCCHHHHHCCCHHH
VRSGLLFVWHDHEGNPPDPAVRIPEIPEAASDEWTDWRWNRILIEGSNCRDIIDNVTDMA
HHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
HFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDVDDLGTSYGEAHLDSEASYFGPSFMIN
HHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHCCCCCCCCCHHHHHH
WLHNRYGNYKSESILINCHYPVTQNSFVLQWGVIVEKPKGMSEEMTDKLSRVFTEGVSKG
HHHHHCCCCCCCEEEEEECCCCCCCCEEEEECEEEECCCCCCHHHHHHHHHHHHHHHHHH
FLQDVEIWKHKTRIDNPLLVEEDGAVYQLRRWYEQFYVDVADIKPEMVERFEIEVDTKRA
HHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHEEHHHCCHHHHHHEEEEECHHHH
NEFWNAEVEKNLKSREVSDDVPAEQH
HHHHCCHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
STDTSGVGVREIDAGALPTRYARGWHCLGVAKDYLEGKPHGVEAFGTKLVVFADSHGDL
CCCCCCCCEEECCCCCCCHHHCCCEEEEHHHHHHHCCCCCCCCCCCCEEEEEECCCCCC
KVLDGYCRHMGGDLSEGTVKGDEVACPFHDWRWGGDGRCKLVPYARRTPRMARTRSWTTD
HHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEECCHHCCCHHHHHCCCHHH
VRSGLLFVWHDHEGNPPDPAVRIPEIPEAASDEWTDWRWNRILIEGSNCRDIIDNVTDMA
HHCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHH
HFFYIHFGLPTYFKNVFEGHIASQYLHNVGRPDVDDLGTSYGEAHLDSEASYFGPSFMIN
HHHHHEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHCCCCCCCCCHHHHHH
WLHNRYGNYKSESILINCHYPVTQNSFVLQWGVIVEKPKGMSEEMTDKLSRVFTEGVSKG
HHHHHCCCCCCCEEEEEECCCCCCCCEEEEECEEEECCCCCCHHHHHHHHHHHHHHHHHH
FLQDVEIWKHKTRIDNPLLVEEDGAVYQLRRWYEQFYVDVADIKPEMVERFEIEVDTKRA
HHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHEEHHHCCHHHHHHEEEEECHHHH
NEFWNAEVEKNLKSREVSDDVPAEQH
HHHHCCHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230