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The map label for this gene is 121639298

Identifier: 121639298

GI number: 121639298

Start: 3772496

End: 3773836

Strand: Direct

Name: 121639298

Synonym: BCG_3443

Alternate gene names: NA

Gene position: 3772496-3773836 (Clockwise)

Preceding gene: 121639296

Following gene: 121639299

Centisome position: 86.24

GC content: 66.07

Gene sequence:

>1341_bases
ATGGCACAACTGACGGCACTGGATGCGGGTTTTCTCAAGTCCCGCGATCCGGAGCGGCACCCGGGCCTGGCGATCGGCGC
AGTTGCCGTCGTCAACGGTGCCGCCCCCAGCTACGACCAGCTCAAAACGGTTCTCACAGAACGGATTAAGTCGATACCTC
GATGTACCCAGGTGTTGGCGACCGAGTGGATCGACTATCCGGGATTCGACCTCACCCAGCACGTGCGACGGGTGGCGCTT
CCCCGGCCCGGCGACGAAGCCGAGCTGTTCCGGGCCATCGCGCTGGCACTGGAGCGTCCCCTCGACCCGGACCGCCCGCT
GTGGGAATGCTGGATCATCGAAGGCCTCAACGGCAACCGCTGGGCGATCTTGATAAAAATCCACCATTGCATGGCCGGCG
CCATGTCGGCGGCCCACCTGCTGGCCAGGCTCTGCGACGATGCCGACGGCAGTGCCTTCGCTAACAATGTTGATATCAAA
CAGATTCCGCCGTATGGCGATGCGCGGAGCTGGGCCGAAACGCTGTGGCGAATGTCCGTCAGCATCGCTGGCGCCGTCTG
CACGGCCGCGGCACGCGCCGTCAGCTGGCCGGCAGTGACGTCACCGGCCGGCCCGGTCACCACCAGGCGGCGGTACCAAG
CGGTGCGCGTTCCCCGCGACGCCGTCGACGCCGTGTGCCACAAGTTCGGGGTGACCGCCAACGACGTCGCGCTCGCGGCC
ATCACCGAGGGCTTCCGAACGGTTCTGCTGCACCGCGGCCAGCAACCGCGCGCCGACTCACTGCGTACCCTGGAGAAAAC
CGATGGCAGCTCGGCCATGCTGCCCTATCTCCCCGTCGAGTACGACGACCCGGTGCGGCGATTGCGCACCGTGCACAACC
GGTCACAGCAGAGCGGCCGTCGTCAACCCGACAGTCTGTCGGACTATACGCCTCTCATGTTGTGCGCCAAGATGATTCAC
GCGCTAGCTCGGTTACCGCAACAAGGCATCGTCACCCTGGCGACCAGTGCACCCGGGCCACGCCACCAGTTACGGCTGAT
GGGCCAGAAGATGGACCAGGTGCTGCCCATCCCGCCCACCGCACTGCAGCTGAGCACCGGGGTCGCGGTCCTCAGCTACG
GCGATGAGCTGGTGTTCGGCATCACCGCTGACTATGACGCCGCGTCCGAAATGCAGCAGCTGGTCAACGGTATCGAACTG
GGTGTGGCGCGTCTGGTGGCGCTCAGCGACGATTCCGTGCTGCTGTTTACCAAGGATCGGCGTAAGCGTTCATCCCGCGC
ACTCCCCAGCGCCGCGCGGCGGGGGCGGCCCTCTGTGCCGACCGCCCGAGCGCGTCACTGA

Upstream 100 bases:

>100_bases
CACCTGCGAAACATGTTCTTAACCGTTTGGGCCTTCTGCTTCCGGTGCGGTCCGGCGGACACTTATACCTGGGGTCGCAA
AACGACGGTGGGGACTTGTC

Downstream 100 bases:

>100_bases
CGCCATCTCCGTCGGCGTTGACCCCCGTGAGAGGGTGGGTCGTGCGCAAGTTGGGCCCGGTCACCATCGATCCGCGCCGC
CATGACGCGGTGCTGTTCGA

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv3371/MT3481 [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MAQLTALDAGFLKSRDPERHPGLAIGAVAVVNGAAPSYDQLKTVLTERIKSIPRCTQVLATEWIDYPGFDLTQHVRRVAL
PRPGDEAELFRAIALALERPLDPDRPLWECWIIEGLNGNRWAILIKIHHCMAGAMSAAHLLARLCDDADGSAFANNVDIK
QIPPYGDARSWAETLWRMSVSIAGAVCTAAARAVSWPAVTSPAGPVTTRRRYQAVRVPRDAVDAVCHKFGVTANDVALAA
ITEGFRTVLLHRGQQPRADSLRTLEKTDGSSAMLPYLPVEYDDPVRRLRTVHNRSQQSGRRQPDSLSDYTPLMLCAKMIH
ALARLPQQGIVTLATSAPGPRHQLRLMGQKMDQVLPIPPTALQLSTGVAVLSYGDELVFGITADYDAASEMQQLVNGIEL
GVARLVALSDDSVLLFTKDRRKRSSRALPSAARRGRPSVPTARARH

Sequences:

>Translated_446_residues
MAQLTALDAGFLKSRDPERHPGLAIGAVAVVNGAAPSYDQLKTVLTERIKSIPRCTQVLATEWIDYPGFDLTQHVRRVAL
PRPGDEAELFRAIALALERPLDPDRPLWECWIIEGLNGNRWAILIKIHHCMAGAMSAAHLLARLCDDADGSAFANNVDIK
QIPPYGDARSWAETLWRMSVSIAGAVCTAAARAVSWPAVTSPAGPVTTRRRYQAVRVPRDAVDAVCHKFGVTANDVALAA
ITEGFRTVLLHRGQQPRADSLRTLEKTDGSSAMLPYLPVEYDDPVRRLRTVHNRSQQSGRRQPDSLSDYTPLMLCAKMIH
ALARLPQQGIVTLATSAPGPRHQLRLMGQKMDQVLPIPPTALQLSTGVAVLSYGDELVFGITADYDAASEMQQLVNGIEL
GVARLVALSDDSVLLFTKDRRKRSSRALPSAARRGRPSVPTARARH
>Mature_445_residues
AQLTALDAGFLKSRDPERHPGLAIGAVAVVNGAAPSYDQLKTVLTERIKSIPRCTQVLATEWIDYPGFDLTQHVRRVALP
RPGDEAELFRAIALALERPLDPDRPLWECWIIEGLNGNRWAILIKIHHCMAGAMSAAHLLARLCDDADGSAFANNVDIKQ
IPPYGDARSWAETLWRMSVSIAGAVCTAAARAVSWPAVTSPAGPVTTRRRYQAVRVPRDAVDAVCHKFGVTANDVALAAI
TEGFRTVLLHRGQQPRADSLRTLEKTDGSSAMLPYLPVEYDDPVRRLRTVHNRSQQSGRRQPDSLSDYTPLMLCAKMIHA
LARLPQQGIVTLATSAPGPRHQLRLMGQKMDQVLPIPPTALQLSTGVAVLSYGDELVFGITADYDAASEMQQLVNGIELG
VARLVALSDDSVLLFTKDRRKRSSRALPSAARRGRPSVPTARARH

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity to produce wax esters [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 48735; Mature: 48603

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLTALDAGFLKSRDPERHPGLAIGAVAVVNGAAPSYDQLKTVLTERIKSIPRCTQVLA
CCCCCCCCHHHHHCCCCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TEWIDYPGFDLTQHVRRVALPRPGDEAELFRAIALALERPLDPDRPLWECWIIEGLNGNR
HHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCE
WAILIKIHHCMAGAMSAAHLLARLCDDADGSAFANNVDIKQIPPYGDARSWAETLWRMSV
EEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SIAGAVCTAAARAVSWPAVTSPAGPVTTRRRYQAVRVPRDAVDAVCHKFGVTANDVALAA
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHH
ITEGFRTVLLHRGQQPRADSLRTLEKTDGSSAMLPYLPVEYDDPVRRLRTVHNRSQQSGR
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
RQPDSLSDYTPLMLCAKMIHALARLPQQGIVTLATSAPGPRHQLRLMGQKMDQVLPIPPT
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCC
ALQLSTGVAVLSYGDELVFGITADYDAASEMQQLVNGIELGVARLVALSDDSVLLFTKDR
CEEHHCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCH
RKRSSRALPSAARRGRPSVPTARARH
HHHHHHCCCHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AQLTALDAGFLKSRDPERHPGLAIGAVAVVNGAAPSYDQLKTVLTERIKSIPRCTQVLA
CCCCCCCHHHHHCCCCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TEWIDYPGFDLTQHVRRVALPRPGDEAELFRAIALALERPLDPDRPLWECWIIEGLNGNR
HHHCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCE
WAILIKIHHCMAGAMSAAHLLARLCDDADGSAFANNVDIKQIPPYGDARSWAETLWRMSV
EEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
SIAGAVCTAAARAVSWPAVTSPAGPVTTRRRYQAVRVPRDAVDAVCHKFGVTANDVALAA
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHH
ITEGFRTVLLHRGQQPRADSLRTLEKTDGSSAMLPYLPVEYDDPVRRLRTVHNRSQQSGR
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
RQPDSLSDYTPLMLCAKMIHALARLPQQGIVTLATSAPGPRHQLRLMGQKMDQVLPIPPT
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCC
ALQLSTGVAVLSYGDELVFGITADYDAASEMQQLVNGIELGVARLVALSDDSVLLFTKDR
CEEHHCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHEEECCCCEEEEECCH
RKRSSRALPSAARRGRPSVPTARARH
HHHHHHCCCHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]