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The map label for this gene is otsB2

Identifier: 121639299

GI number: 121639299

Start: 3773878

End: 3775053

Strand: Direct

Name: otsB2

Synonym: BCG_3444

Alternate gene names: 121639299

Gene position: 3773878-3775053 (Clockwise)

Preceding gene: 121639298

Following gene: 121639300

Centisome position: 86.27

GC content: 68.88

Gene sequence:

>1176_bases
GTGCGCAAGTTGGGCCCGGTCACCATCGATCCGCGCCGCCATGACGCGGTGCTGTTCGACACCACGTTGGACGCCACCCA
GGAACTGGTCCGGCAACTCCAGGAAGTCGGTGTGGGCACCGGCGTCTTCGGTAGTGGCCTAGACGTTCCGATCGTAGCGG
CCGGCCGTCTGGCGGTGCGGCCGGGCCGGTGCGTGGTCGTCTCGGCCCACTCGGCGGGCGTCACGGCCGCACGCGAAAGC
GGATTTGCGCTGATCATCGGTGTCGACCGCACCGGGTGTCGGGACGCATTGCGTCGCGACGGCGCCGACACGGTGGTCAC
CGACCTAAGCGAGGTCAGCGTGCGCACCGGGGACCGACGCATGTCGCAGCTGCCCGACGCGTTACAGGCACTCGGCCTGG
CCGACGGCCTGGTCGCCCGGCAGCCCGCGGTGTTCTTCGACTTCGACGGCACGCTGTCCGACATTGTCGAGGATCCCGAC
GCGGCCTGGCTCGCCCCCGGTGCCTTGGAGGCACTGCAGAAGTTGGCCGCGCGCTGTCCGATCGCGGTGCTCAGTGGCCG
CGACCTGGCCGACGTGACACAGCGGGTGGGTCTGCCCGGCATCTGGTATGCCGGCAGCCATGGTTTCGAATTGACCGCAC
CCGACGGAACGCACCACCAGAACGACGCCGCGGCGGCAGCCATACCGGTGCTGAAACAGGCGGCTGCCGAGCTGCGCCAG
CAACTTGGACCCTTCCCGGGTGTTGTGGTGGAGCACAAGCGGTTTGGCGTCGCCGTGCACTACCGCAACGCGGCCCGGGA
CCGGGTCGGCGAAGTCGCCGCGGCGGTGCGCACGGCCGAGCAGCGTCATGCGCTGCGGGTGACGACGGGCCGCGAAGTCA
TCGAGTTGCGTCCCGATGTCGACTGGGACAAGGGGAAAACGCTGCTGTGGGTTCTTGACCATCTGCCGCATTCGGGCTCG
GCTCCCCTGGTGCCGATCTACCTCGGCGACGACATCACCGACGAGGACGCTTTCGATGTGGTCGGCCCCCATGGTGTTCC
AATTGTGGTGCGCCACACCGACGACGGTGACCGCGCCACCGCCGCACTGTTTGCGCTGGACAGTCCCGCACGGGTCGCGG
AGTTCACCGATCGGCTGGCGCGTCAGCTCCGTGAGGCTCCCCTGCGGGCAACGTGA

Upstream 100 bases:

>100_bases
TCCCCAGCGCCGCGCGGCGGGGGCGGCCCTCTGTGCCGACCGCCCGAGCGCGTCACTGACGCCATCTCCGTCGGCGTTGA
CCCCCGTGAGAGGGTGGGTC

Downstream 100 bases:

>100_bases
GACGCGGTGCCGCCGCGGGCGATACGCTCCGACCGTCAACGAGGAGGACGGCCATGTGGTTTGCATTGGTGAACCCGGAG
ATGCTGGCCGCGGCGGCGAC

Product: putative trehalose 6-phosphate phosphatase otsB2

Products: NA

Alternate protein names: TPP; Trehalose-6-phosphate phosphatase

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MRKLGPVTIDPRRHDAVLFDTTLDATQELVRQLQEVGVGTGVFGSGLDVPIVAAGRLAVRPGRCVVVSAHSAGVTAARES
GFALIIGVDRTGCRDALRRDGADTVVTDLSEVSVRTGDRRMSQLPDALQALGLADGLVARQPAVFFDFDGTLSDIVEDPD
AAWLAPGALEALQKLAARCPIAVLSGRDLADVTQRVGLPGIWYAGSHGFELTAPDGTHHQNDAAAAAIPVLKQAAAELRQ
QLGPFPGVVVEHKRFGVAVHYRNAARDRVGEVAAAVRTAEQRHALRVTTGREVIELRPDVDWDKGKTLLWVLDHLPHSGS
APLVPIYLGDDITDEDAFDVVGPHGVPIVVRHTDDGDRATAALFALDSPARVAEFTDRLARQLREAPLRAT

Sequences:

>Translated_391_residues
MRKLGPVTIDPRRHDAVLFDTTLDATQELVRQLQEVGVGTGVFGSGLDVPIVAAGRLAVRPGRCVVVSAHSAGVTAARES
GFALIIGVDRTGCRDALRRDGADTVVTDLSEVSVRTGDRRMSQLPDALQALGLADGLVARQPAVFFDFDGTLSDIVEDPD
AAWLAPGALEALQKLAARCPIAVLSGRDLADVTQRVGLPGIWYAGSHGFELTAPDGTHHQNDAAAAAIPVLKQAAAELRQ
QLGPFPGVVVEHKRFGVAVHYRNAARDRVGEVAAAVRTAEQRHALRVTTGREVIELRPDVDWDKGKTLLWVLDHLPHSGS
APLVPIYLGDDITDEDAFDVVGPHGVPIVVRHTDDGDRATAALFALDSPARVAEFTDRLARQLREAPLRAT
>Mature_391_residues
MRKLGPVTIDPRRHDAVLFDTTLDATQELVRQLQEVGVGTGVFGSGLDVPIVAAGRLAVRPGRCVVVSAHSAGVTAARES
GFALIIGVDRTGCRDALRRDGADTVVTDLSEVSVRTGDRRMSQLPDALQALGLADGLVARQPAVFFDFDGTLSDIVEDPD
AAWLAPGALEALQKLAARCPIAVLSGRDLADVTQRVGLPGIWYAGSHGFELTAPDGTHHQNDAAAAAIPVLKQAAAELRQ
QLGPFPGVVVEHKRFGVAVHYRNAARDRVGEVAAAVRTAEQRHALRVTTGREVIELRPDVDWDKGKTLLWVLDHLPHSGS
APLVPIYLGDDITDEDAFDVVGPHGVPIVVRHTDDGDRATAALFALDSPARVAEFTDRLARQLREAPLRAT

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family

Homologues:

Organism=Escherichia coli, GI1788207, Length=205, Percent_Identity=31.7073170731707, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI221473368, Length=269, Percent_Identity=32.3420074349442, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI20129307, Length=269, Percent_Identity=32.3420074349442, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24582490, Length=269, Percent_Identity=32.3420074349442, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19920676, Length=252, Percent_Identity=30.1587301587302, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129309, Length=236, Percent_Identity=27.9661016949153, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OTSB_MYCBO (Q7TWL7)

Other databases:

- EMBL:   BX248345
- RefSeq:   NP_857048.1
- ProteinModelPortal:   Q7TWL7
- SMR:   Q7TWL7
- EnsemblBacteria:   EBMYCT00000014763
- GeneID:   1090943
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3407
- GeneTree:   EBGT00050000017221
- HOGENOM:   HBG686849
- OMA:   ATHYRNA
- ProtClustDB:   CLSK792454
- BioCyc:   MBOV233413:MB3407-MONOMER
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR01484
- TIGRFAMs:   TIGR00685

Pfam domain/function: PF02358 Trehalose_PPase; SSF56784 SSF56784

EC number: =3.1.3.12

Molecular weight: Translated: 41721; Mature: 41721

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLGPVTIDPRRHDAVLFDTTLDATQELVRQLQEVGVGTGVFGSGLDVPIVAAGRLAVR
CCCCCCEEECCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEEEC
PGRCVVVSAHSAGVTAARESGFALIIGVDRTGCRDALRRDGADTVVTDLSEVSVRTGDRR
CCCEEEEECCCCCCEEECCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHH
MSQLPDALQALGLADGLVARQPAVFFDFDGTLSDIVEDPDAAWLAPGALEALQKLAARCP
HHHHHHHHHHHHHHCCHHHCCCEEEEECCCCHHHHHCCCCCCEECCHHHHHHHHHHHHCC
IAVLSGRDLADVTQRVGLPGIWYAGSHGFELTAPDGTHHQNDAAAAAIPVLKQAAAELRQ
EEEECCCCHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
QLGPFPGVVVEHKRFGVAVHYRNAARDRVGEVAAAVRTAEQRHALRVTTGREVIELRPDV
HHCCCCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEECCCC
DWDKGKTLLWVLDHLPHSGSAPLVPIYLGDDITDEDAFDVVGPHGVPIVVRHTDDGDRAT
CCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCE
AALFALDSPARVAEFTDRLARQLREAPLRAT
EEEEEECCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRKLGPVTIDPRRHDAVLFDTTLDATQELVRQLQEVGVGTGVFGSGLDVPIVAAGRLAVR
CCCCCCEEECCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEEEEC
PGRCVVVSAHSAGVTAARESGFALIIGVDRTGCRDALRRDGADTVVTDLSEVSVRTGDRR
CCCEEEEECCCCCCEEECCCCCEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHH
MSQLPDALQALGLADGLVARQPAVFFDFDGTLSDIVEDPDAAWLAPGALEALQKLAARCP
HHHHHHHHHHHHHHCCHHHCCCEEEEECCCCHHHHHCCCCCCEECCHHHHHHHHHHHHCC
IAVLSGRDLADVTQRVGLPGIWYAGSHGFELTAPDGTHHQNDAAAAAIPVLKQAAAELRQ
EEEECCCCHHHHHHHCCCCEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
QLGPFPGVVVEHKRFGVAVHYRNAARDRVGEVAAAVRTAEQRHALRVTTGREVIELRPDV
HHCCCCCEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHCEEEEECCCEEEEECCCC
DWDKGKTLLWVLDHLPHSGSAPLVPIYLGDDITDEDAFDVVGPHGVPIVVRHTDDGDRAT
CCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCE
AALFALDSPARVAEFTDRLARQLREAPLRAT
EEEEEECCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972