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The map label for this gene is dhmA1

Identifier: 121638177

GI number: 121638177

Start: 2544599

End: 2545501

Strand: Direct

Name: dhmA1

Synonym: BCG_2312

Alternate gene names: 121638177

Gene position: 2544599-2545501 (Clockwise)

Preceding gene: 121638176

Following gene: 121638178

Centisome position: 58.17

GC content: 66.33

Gene sequence:

>903_bases
ATGGATGTCCTACGCACCCCAGACTCCCGGTTCGAACACCTGGTGGGCTACCCGTTTGCACCGCACTATGTCGATGTGAC
GGCCGGCGACACCCAGCCGTTGCGAATGCACTACGTCGACGAGGGCCCGGGCGACGGTCCGCCGATCGTCTTGCTGCACG
GCGAGCCCACCTGGAGTTATCTGTACCGAACCATGATTCCGCCGCTCTCCGCCGCCGGGCACCGTGTGCTCGCGCCCGAC
CTGATCGGCTTCGGCCGCTCCGACAAGCCGACTCGCATCGAGGACTACACCTACCTGCGGCACGTCGAGTGGGTGACGTC
CTGGTTCGAGAATCTCGACCTGCACGACGTTACGCTCTTCGTGCAGGACTGGGGGTCATTGATCGGTCTGCGCATCGCTG
CCGAGCACGGTGACCGGATCGCGCGGCTGGTGGTCGCCAACGGGTTTCTCCCCGCCGCGCAGGGGCGCACCCCACTCCCC
TTCTACGTGTGGCGGGCGTTTGCGCGCTATTCTCCGGTGCTTCCCGCTGGCCGTCTGGTGAACTTCGGCACCGTCCACAG
GGTTCCCGCCGGGGTCCGAGCCGGCTACGATGCACCTTTCCCCGACAAAACGTATCAAGCCGGCGCCCGGGCGTTCCCAC
GGTTGGTGCCGACCTCACCCGACGATCCGGCGGTACCGGCCAACCGCGCGGCATGGGAAGCCCTGGGCCGGTGGGACAAA
CCGTTCCTTGCCATCTTCGGTTATCGCGACCCGATACTCGGGCAAGCGGACGGTCCGCTGATCAAGCACATTCCCGGCGC
GGCGGGTCAGCCGCACGCCCGCATCAAGGCCAGCCACTTCATCCAGGAGGACAGCGGAACCGAACTCGCCGAACGCATGC
TCTCCTGGCAGCAGGCAACGTAA

Upstream 100 bases:

>100_bases
CGCTTAACCTCGGCGGCGACTGAGTCGACGCGAGCGCGGATATCGGACGCTTTTGCACAACAATGGTTCCGACGTGGCAC
AGCTCAGAGAGGAGCAGATC

Downstream 100 bases:

>100_bases
CCGCGACGGCTGCGGACGAAGGATCGGCAGAATGGCGATGGAGATGGCGATGATGGGCCTGCTCGGCACCGTGGTGGGTG
CCTCGGCCATGGGCATCGGG

Product: haloalkane dehalogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MDVLRTPDSRFEHLVGYPFAPHYVDVTAGDTQPLRMHYVDEGPGDGPPIVLLHGEPTWSYLYRTMIPPLSAAGHRVLAPD
LIGFGRSDKPTRIEDYTYLRHVEWVTSWFENLDLHDVTLFVQDWGSLIGLRIAAEHGDRIARLVVANGFLPAAQGRTPLP
FYVWRAFARYSPVLPAGRLVNFGTVHRVPAGVRAGYDAPFPDKTYQAGARAFPRLVPTSPDDPAVPANRAAWEALGRWDK
PFLAIFGYRDPILGQADGPLIKHIPGAAGQPHARIKASHFIQEDSGTELAERMLSWQQAT

Sequences:

>Translated_300_residues
MDVLRTPDSRFEHLVGYPFAPHYVDVTAGDTQPLRMHYVDEGPGDGPPIVLLHGEPTWSYLYRTMIPPLSAAGHRVLAPD
LIGFGRSDKPTRIEDYTYLRHVEWVTSWFENLDLHDVTLFVQDWGSLIGLRIAAEHGDRIARLVVANGFLPAAQGRTPLP
FYVWRAFARYSPVLPAGRLVNFGTVHRVPAGVRAGYDAPFPDKTYQAGARAFPRLVPTSPDDPAVPANRAAWEALGRWDK
PFLAIFGYRDPILGQADGPLIKHIPGAAGQPHARIKASHFIQEDSGTELAERMLSWQQAT
>Mature_300_residues
MDVLRTPDSRFEHLVGYPFAPHYVDVTAGDTQPLRMHYVDEGPGDGPPIVLLHGEPTWSYLYRTMIPPLSAAGHRVLAPD
LIGFGRSDKPTRIEDYTYLRHVEWVTSWFENLDLHDVTLFVQDWGSLIGLRIAAEHGDRIARLVVANGFLPAAQGRTPLP
FYVWRAFARYSPVLPAGRLVNFGTVHRVPAGVRAGYDAPFPDKTYQAGARAFPRLVPTSPDDPAVPANRAAWEALGRWDK
PFLAIFGYRDPILGQADGPLIKHIPGAAGQPHARIKASHFIQEDSGTELAERMLSWQQAT

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI27597073, Length=127, Percent_Identity=33.0708661417323, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17562298, Length=116, Percent_Identity=37.9310344827586, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324392, Length=127, Percent_Identity=31.496062992126, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DHMA1_MYCBO (P64302)

Other databases:

- EMBL:   BX248342
- RefSeq:   NP_855967.1
- ProteinModelPortal:   P64302
- SMR:   P64302
- EnsemblBacteria:   EBMYCT00000016210
- GeneID:   1090414
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2318
- GeneTree:   EBGT00050000015214
- HOGENOM:   HBG748003
- OMA:   PHARINA
- ProtClustDB:   PRK00870
- BioCyc:   MBOV233413:MB2318-MONOMER
- BRENDA:   3.8.1.5
- HAMAP:   MF_01230
- InterPro:   IPR000073
- InterPro:   IPR000639
- PRINTS:   PR00111
- PRINTS:   PR00412

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =3.8.1.5

Molecular weight: Translated: 33358; Mature: 33358

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: ACT_SITE 123-123 ACT_SITE 250-250 ACT_SITE 279-279

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVLRTPDSRFEHLVGYPFAPHYVDVTAGDTQPLRMHYVDEGPGDGPPIVLLHGEPTWSY
CCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCEEEEECCCHHHH
LYRTMIPPLSAAGHRVLAPDLIGFGRSDKPTRIEDYTYLRHVEWVTSWFENLDLHDVTLF
HHHHHCCCCCCCCCEEECHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHEEEE
VQDWGSLIGLRIAAEHGDRIARLVVANGFLPAAQGRTPLPFYVWRAFARYSPVLPAGRLV
HHHHHHHHEEEEEECCCCCEEEEEHCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCEE
NFGTVHRVPAGVRAGYDAPFPDKTYQAGARAFPRLVPTSPDDPAVPANRAAWEALGRWDK
CCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
PFLAIFGYRDPILGQADGPLIKHIPGAAGQPHARIKASHFIQEDSGTELAERMLSWQQAT
CEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDVLRTPDSRFEHLVGYPFAPHYVDVTAGDTQPLRMHYVDEGPGDGPPIVLLHGEPTWSY
CCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCCEEEEECCCHHHH
LYRTMIPPLSAAGHRVLAPDLIGFGRSDKPTRIEDYTYLRHVEWVTSWFENLDLHDVTLF
HHHHHCCCCCCCCCEEECHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCHHEEEE
VQDWGSLIGLRIAAEHGDRIARLVVANGFLPAAQGRTPLPFYVWRAFARYSPVLPAGRLV
HHHHHHHHEEEEEECCCCCEEEEEHCCCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCEE
NFGTVHRVPAGVRAGYDAPFPDKTYQAGARAFPRLVPTSPDDPAVPANRAAWEALGRWDK
CCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCC
PFLAIFGYRDPILGQADGPLIKHIPGAAGQPHARIKASHFIQEDSGTELAERMLSWQQAT
CEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972