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The map label for this gene is dagK

Identifier: 121638135

GI number: 121638135

Start: 2505549

End: 2506478

Strand: Direct

Name: dagK

Synonym: BCG_2270

Alternate gene names: 121638135

Gene position: 2505549-2506478 (Clockwise)

Preceding gene: 121638134

Following gene: 121638136

Centisome position: 57.28

GC content: 63.87

Gene sequence:

>930_bases
ATGAGCGCCGGGCAGCTGCGCCGGCATGAGATCGGCAAGGTCACCGCGCTGACCAATCCCCTGTCAGGCCATGGCGCCGC
CGTAAAGGCTGCACACGGCGCGATCGCCCGGCTGAAGCATCGGGGGGTGGACGTCGTCGAGATCGTCGGCGGGGACGCCC
ACGACGCACGCCATCTGCTCGCCGCGGCAGTCGCAAAAGGCACTGACGCGGTGATGGTGACCGGCGGTGACGGAGTCGTC
TCCAACGCGCTACAGGTCTTGGCGGGCACCGACATTCCGTTAGGAATCATTCCGGCCGGCACTGGTAACGACCACGCACG
CGAATTCGGGCTTCCCACAAAGAATCCCAAGGCAGCCGCAGATATCGTTGTTGACGGCTGGACGGAAACCATTGACCTGG
GCCGGATTCAAGACGACAACGGTATCGAAAAGTGGTTCGGTACCGTGGCGGCTACCGGATTCGACTCCCTGGTCAACGAT
CGCGCCAACCGAATGCGCTGGCCACACGGGCGGATGCGCTATTACATCGCGATGCTCGCCGAACTGTCGCGGCTGCGGCC
GTTGCCGTTCCGGCTGGTGCTCGACGGCACCGAAGAGATCGTCGCCGACCTCACACTTGCCGACTTCGGCAATACCCGCA
GCTACGGCGGCGGATTATTGATCTGCCCCAACGCCGACCACTCGGACGGCCTGCTCGACATCACCATGGCCCAGTCGGAT
TCCCGTACCAAGTTGCTCCGCCTGTTCCCCACCATTTTCAAAGGCGCCCATGTCGAGCTTGACGAGGTGAGCACCACACG
AGCCAAGACAGTCCACGTCGAGTGCCCCGGTATCAACGTCTATGCCGACGGCGACTTCGCCTGCCCGTTACCAGCCGAGA
TCTCCGCGGTGCCGGCCGCCCTTCAGGTTCTTCGCCCCCGCCACGGATAA

Upstream 100 bases:

>100_bases
GGATGCGCGCGGAGGTGGGTGATCTGGGCGTGACATTGTTGCGCACGATCAAGGCGACGCTGGATCCGGCCGGAATTCTC
AACCCCGGCAAGCTGATTCC

Downstream 100 bases:

>100_bases
GCGGGTGGTAACGACTCGGTCGTAAAGCGCGACATCCTTCCAAACCCGCTGTACGGGAGGAACAGATGTCCGGACACCGC
AAGAAGGCAATGCTCGCCTT

Product: diacylglycerol kinase

Products: NA

Alternate protein names: DAG kinase; DAGK

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSAGQLRRHEIGKVTALTNPLSGHGAAVKAAHGAIARLKHRGVDVVEIVGGDAHDARHLLAAAVAKGTDAVMVTGGDGVV
SNALQVLAGTDIPLGIIPAGTGNDHAREFGLPTKNPKAAADIVVDGWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVND
RANRMRWPHGRMRYYIAMLAELSRLRPLPFRLVLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHSDGLLDITMAQSD
SRTKLLRLFPTIFKGAHVELDEVSTTRAKTVHVECPGINVYADGDFACPLPAEISAVPAALQVLRPRHG

Sequences:

>Translated_309_residues
MSAGQLRRHEIGKVTALTNPLSGHGAAVKAAHGAIARLKHRGVDVVEIVGGDAHDARHLLAAAVAKGTDAVMVTGGDGVV
SNALQVLAGTDIPLGIIPAGTGNDHAREFGLPTKNPKAAADIVVDGWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVND
RANRMRWPHGRMRYYIAMLAELSRLRPLPFRLVLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHSDGLLDITMAQSD
SRTKLLRLFPTIFKGAHVELDEVSTTRAKTVHVECPGINVYADGDFACPLPAEISAVPAALQVLRPRHG
>Mature_308_residues
SAGQLRRHEIGKVTALTNPLSGHGAAVKAAHGAIARLKHRGVDVVEIVGGDAHDARHLLAAAVAKGTDAVMVTGGDGVVS
NALQVLAGTDIPLGIIPAGTGNDHAREFGLPTKNPKAAADIVVDGWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVNDR
ANRMRWPHGRMRYYIAMLAELSRLRPLPFRLVLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHSDGLLDITMAQSDS
RTKLLRLFPTIFKGAHVELDEVSTTRAKTVHVECPGINVYADGDFACPLPAEISAVPAALQVLRPRHG

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is involved in the biosynthesis of phosphatidylinositol mannosides (PIMs), probably via a role in the biosynthesis of phosphatidylinositol (PI), a PIM precursor, which is derive

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Secreted, cell wall (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain

Homologues:

Organism=Escherichia coli, GI1788402, Length=287, Percent_Identity=28.9198606271777, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAGK_MYCTU (O53526)

Other databases:

- EMBL:   BX842579
- EMBL:   AE000516
- PIR:   H70861
- RefSeq:   NP_216768.1
- ProteinModelPortal:   O53526
- SMR:   O53526
- EnsemblBacteria:   EBMYCT00000000438
- EnsemblBacteria:   EBMYCT00000084093
- GeneID:   888429
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtu:Rv2252
- TIGR:   MT2312
- TubercuList:   Rv2252
- GeneTree:   EBGT00050000016768
- HOGENOM:   HBG698136
- OMA:   ESHALAQ
- ProtClustDB:   PRK11914
- InterPro:   IPR005218
- InterPro:   IPR001206
- SMART:   SM00046
- TIGRFAMs:   TIGR00147

Pfam domain/function: PF00781 DAGK_cat

EC number: =2.7.1.107

Molecular weight: Translated: 32806; Mature: 32675

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS50146 DAGK

Important sites: ACT_SITE 285-285 BINDING 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGQLRRHEIGKVTALTNPLSGHGAAVKAAHGAIARLKHRGVDVVEIVGGDAHDARHLL
CCCCCCHHHHCCEEEEECCCCCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
AAAVAKGTDAVMVTGGDGVVSNALQVLAGTDIPLGIIPAGTGNDHAREFGLPTKNPKAAA
HHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCCEE
DIVVDGWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVNDRANRMRWPHGRMRYYIAMLA
EEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ELSRLRPLPFRLVLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHSDGLLDITMAQSD
HHHHCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCC
SRTKLLRLFPTIFKGAHVELDEVSTTRAKTVHVECPGINVYADGDFACPLPAEISAVPAA
CHHHHHHHHHHHHCCCCEEHHCCCCCCEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHH
LQVLRPRHG
HHHHCCCCC
>Mature Secondary Structure 
SAGQLRRHEIGKVTALTNPLSGHGAAVKAAHGAIARLKHRGVDVVEIVGGDAHDARHLL
CCCCCHHHHCCEEEEECCCCCCCCCEEHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
AAAVAKGTDAVMVTGGDGVVSNALQVLAGTDIPLGIIPAGTGNDHAREFGLPTKNPKAAA
HHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHCCCCCCCCCCEE
DIVVDGWTETIDLGRIQDDNGIEKWFGTVAATGFDSLVNDRANRMRWPHGRMRYYIAMLA
EEEEECCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ELSRLRPLPFRLVLDGTEEIVADLTLADFGNTRSYGGGLLICPNADHSDGLLDITMAQSD
HHHHCCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEEEEEECCCC
SRTKLLRLFPTIFKGAHVELDEVSTTRAKTVHVECPGINVYADGDFACPLPAEISAVPAA
CHHHHHHHHHHHHCCCCEEHHCCCCCCEEEEEEECCCEEEEECCCCCCCCCCHHHHHHHH
LQVLRPRHG
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036