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The map label for this gene is murF

Identifier: 121638039

GI number: 121638039

Start: 2397482

End: 2399014

Strand: Reverse

Name: murF

Synonym: BCG_2174c

Alternate gene names: 121638039

Gene position: 2399014-2397482 (Counterclockwise)

Preceding gene: 121638040

Following gene: 121638038

Centisome position: 54.84

GC content: 71.56

Gene sequence:

>1533_bases
ATGATCGAGCTGACCGTCGCGCAGATCGCCGAGATCGTCGGGGGCGCAGTGGCCGATATCTCCCCGCAAGACGCCGCGCA
CCGCCGCGTCACCGGGACCGTCGAGTTCGACTCGCGCGCCATCGGCCCGGGCGGGCTGTTCCTCGCCCTGCCGGGGGCGC
GCGCCGACGGCCACGACCATGCCGCGTCGGCGGTAGCCGCGGGCGCCGCCGTCGTGCTGGCCGCCCGCCCGGTGGGGGTG
CCGGCCATCGTGGTTCCGCCAGTGGCCGCGCCGAACGTATTGGCCGGCGTCCTCGAGCACGACAACGACGGGTCGGGGGC
GGCGGTGCTGGCCGCGCTGGCCAAGCTGGCCACCGCGGTGGCCGCGCAGTTGGTGGCCGGCGGGCTCACCATCATCGGGA
TCACCGGCTCGTCGGGCAAGACGTCGACCAAGGACCTGATGGCCGCCGTGCTGGCCCCGCTGGGGGAGGTGGTGGCCCCG
CCCGGATCGTTCAACAACGAGCTGGGTCACCCGTGGACGGTGCTGCGCGCGACGCGGCGCACCGACTACCTGATTTTGGA
GATGGCGGCACGCCATCACGGCAACATCGCCGCGCTCGCCGAGATCGCGCCCCCGTCGATCGGAGTCGTGCTCAACGTCG
GCACCGCACATTTGGGTGAGTTCGGCTCCCGCGAGGTCATCGCACAGACCAAAGCCGAACTGCCGCAGGCTGTTCCGCAT
TCCGGAGCGGTCGTCCTCAACGCTGATGACCCCGCGGTGGCGGCGATGGCCAAGCTGACCGCGGCCCGGGTGGTGCGGGT
CAGCCGGGACAACACCGGTGACGTTTGGGCGGGGCCGGTGTCGCTGGACGAATTGGCCAGGCCGCGCTTTACGCTGCATG
CCCACGATGCCCAAGCCGAGGTCCGACTCGGGGTCTGCGGCGACCACCAGGTCACTAACGCGCTGTGCGCCGCGGCGGTC
GCGCTGGAGTGTGGGGCCAGCGTTGAACAGGTCGCGGCCGCGCTGACCGCGGCGCCGCCGGTGTCGCGGCATCGGATGCA
GGTGACCACCCGCGGCGACGGGGTGACGGTGATCGACGACGCCTACAACGCCAACCCCGACTCCATGCGGGCCGGGCTGC
AGGCGCTGGCCTGGATCGCGCACCAACCCGAGGCCACCCGCCGCAGCTGGGCGGTGCTGGGTGAGATGGCCGAGCTGGGT
GAGGACGCGATAGCCGAGCACGATCGCATCGGCCGGCTCGCGGTGCGCTTAGATGTGTCTCGACTCGTTGTCGTGGGAAC
CGGGAGGTCGATCAGCGCCATGCACCACGGAGCGGTCCTGGAGGGGGCGTGGGGCTCGGGGGAAGCCACTGCTGATCACG
GTGCGGATCGCACGGCCGTCAATGTGGCCGACGGTGACGCCGCCCTGGCACTACTGCGCGCCGAGCTGCGACCCGGGGAT
GTGGTCTTGGTCAAGGCCTCGAACGCGGCCGGGCTGGGTGCGGTGGCCGATGCATTGGTCGCAGACGACACATGCGGGAG
TGTGCGCCCATGA

Upstream 100 bases:

>100_bases
GCAAAGGCCACGAGACCGGGCAACGCGGCGGCGGGCGGGTCCGCCCGTTCGACGACCGGGTGGAGCTGGCTGCCGCGCTA
GAGGCCCTCGAGCGGCGCGC

Downstream 100 bases:

>100_bases
GGCAGATCCTTATCGCCGTTGCCGTAGCGGTGACGGTGTCCATCTTGCTGACCCCGGTGCTGATCCGGTTGTTCACTAAG
CAGGGCTTCGGCCACCAGAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6- diaminopimelate-D-alanyl-D-alanyl ligase MurF

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MIELTVAQIAEIVGGAVADISPQDAAHRRVTGTVEFDSRAIGPGGLFLALPGARADGHDHAASAVAAGAAVVLAARPVGV
PAIVVPPVAAPNVLAGVLEHDNDGSGAAVLAALAKLATAVAAQLVAGGLTIIGITGSSGKTSTKDLMAAVLAPLGEVVAP
PGSFNNELGHPWTVLRATRRTDYLILEMAARHHGNIAALAEIAPPSIGVVLNVGTAHLGEFGSREVIAQTKAELPQAVPH
SGAVVLNADDPAVAAMAKLTAARVVRVSRDNTGDVWAGPVSLDELARPRFTLHAHDAQAEVRLGVCGDHQVTNALCAAAV
ALECGASVEQVAAALTAAPPVSRHRMQVTTRGDGVTVIDDAYNANPDSMRAGLQALAWIAHQPEATRRSWAVLGEMAELG
EDAIAEHDRIGRLAVRLDVSRLVVVGTGRSISAMHHGAVLEGAWGSGEATADHGADRTAVNVADGDAALALLRAELRPGD
VVLVKASNAAGLGAVADALVADDTCGSVRP

Sequences:

>Translated_510_residues
MIELTVAQIAEIVGGAVADISPQDAAHRRVTGTVEFDSRAIGPGGLFLALPGARADGHDHAASAVAAGAAVVLAARPVGV
PAIVVPPVAAPNVLAGVLEHDNDGSGAAVLAALAKLATAVAAQLVAGGLTIIGITGSSGKTSTKDLMAAVLAPLGEVVAP
PGSFNNELGHPWTVLRATRRTDYLILEMAARHHGNIAALAEIAPPSIGVVLNVGTAHLGEFGSREVIAQTKAELPQAVPH
SGAVVLNADDPAVAAMAKLTAARVVRVSRDNTGDVWAGPVSLDELARPRFTLHAHDAQAEVRLGVCGDHQVTNALCAAAV
ALECGASVEQVAAALTAAPPVSRHRMQVTTRGDGVTVIDDAYNANPDSMRAGLQALAWIAHQPEATRRSWAVLGEMAELG
EDAIAEHDRIGRLAVRLDVSRLVVVGTGRSISAMHHGAVLEGAWGSGEATADHGADRTAVNVADGDAALALLRAELRPGD
VVLVKASNAAGLGAVADALVADDTCGSVRP
>Mature_510_residues
MIELTVAQIAEIVGGAVADISPQDAAHRRVTGTVEFDSRAIGPGGLFLALPGARADGHDHAASAVAAGAAVVLAARPVGV
PAIVVPPVAAPNVLAGVLEHDNDGSGAAVLAALAKLATAVAAQLVAGGLTIIGITGSSGKTSTKDLMAAVLAPLGEVVAP
PGSFNNELGHPWTVLRATRRTDYLILEMAARHHGNIAALAEIAPPSIGVVLNVGTAHLGEFGSREVIAQTKAELPQAVPH
SGAVVLNADDPAVAAMAKLTAARVVRVSRDNTGDVWAGPVSLDELARPRFTLHAHDAQAEVRLGVCGDHQVTNALCAAAV
ALECGASVEQVAAALTAAPPVSRHRMQVTTRGDGVTVIDDAYNANPDSMRAGLQALAWIAHQPEATRRSWAVLGEMAELG
EDAIAEHDRIGRLAVRLDVSRLVVVGTGRSISAMHHGAVLEGAWGSGEATADHGADRTAVNVADGDAALALLRAELRPGD
VVLVKASNAAGLGAVADALVADDTCGSVRP

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786274, Length=511, Percent_Identity=31.8982387475538, Blast_Score=172, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURF_MYCBO (P0A5L5)

Other databases:

- EMBL:   BX248341
- RefSeq:   NP_855830.1
- ProteinModelPortal:   P0A5L5
- SMR:   P0A5L5
- EnsemblBacteria:   EBMYCT00000017587
- GeneID:   1091261
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2181c
- GeneTree:   EBGT00050000015779
- HOGENOM:   HBG617121
- OMA:   ANPASMR
- ProtClustDB:   CLSK791663
- BioCyc:   MBOV233413:MB2181C-MONOMER
- BRENDA:   6.3.2.10
- GO:   GO:0005737
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- PANTHER:   PTHR23135:SF3
- TIGRFAMs:   TIGR01143

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.10

Molecular weight: Translated: 51633; Mature: 51633

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIELTVAQIAEIVGGAVADISPQDAAHRRVTGTVEFDSRAIGPGGLFLALPGARADGHDH
CEEEEHHHHHHHHCCCCCCCCCCCHHCCEEEEEEEECCEECCCCCEEEEECCCCCCCCHH
AASAVAAGAAVVLAARPVGVPAIVVPPVAAPNVLAGVLEHDNDGSGAAVLAALAKLATAV
HHHHHHHCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AAQLVAGGLTIIGITGSSGKTSTKDLMAAVLAPLGEVVAPPGSFNNELGHPWTVLRATRR
HHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHCC
TDYLILEMAARHHGNIAALAEIAPPSIGVVLNVGTAHLGEFGSREVIAQTKAELPQAVPH
CCEEEEEEHHHCCCCEEEEEECCCCCCEEEEECCCHHHCCCCCHHHHHHHHHHCCCCCCC
SGAVVLNADDPAVAAMAKLTAARVVRVSRDNTGDVWAGPVSLDELARPRFTLHAHDAQAE
CCEEEEECCCCHHHHHHHHHHHHEEEECCCCCCCEEECCCCHHHHCCCCEEEEECCCCCE
VRLGVCGDHQVTNALCAAAVALECGASVEQVAAALTAAPPVSRHRMQVTTRGDGVTVIDD
EEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEC
AYNANPDSMRAGLQALAWIAHQPEATRRSWAVLGEMAELGEDAIAEHDRIGRLAVRLDVS
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEEEEE
RLVVVGTGRSISAMHHGAVLEGAWGSGEATADHGADRTAVNVADGDAALALLRAELRPGD
EEEEEECCCCCHHHHCCEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCC
VVLVKASNAAGLGAVADALVADDTCGSVRP
EEEEEECCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIELTVAQIAEIVGGAVADISPQDAAHRRVTGTVEFDSRAIGPGGLFLALPGARADGHDH
CEEEEHHHHHHHHCCCCCCCCCCCHHCCEEEEEEEECCEECCCCCEEEEECCCCCCCCHH
AASAVAAGAAVVLAARPVGVPAIVVPPVAAPNVLAGVLEHDNDGSGAAVLAALAKLATAV
HHHHHHHCEEEEEEECCCCCCEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AAQLVAGGLTIIGITGSSGKTSTKDLMAAVLAPLGEVVAPPGSFNNELGHPWTVLRATRR
HHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEHHCC
TDYLILEMAARHHGNIAALAEIAPPSIGVVLNVGTAHLGEFGSREVIAQTKAELPQAVPH
CCEEEEEEHHHCCCCEEEEEECCCCCCEEEEECCCHHHCCCCCHHHHHHHHHHCCCCCCC
SGAVVLNADDPAVAAMAKLTAARVVRVSRDNTGDVWAGPVSLDELARPRFTLHAHDAQAE
CCEEEEECCCCHHHHHHHHHHHHEEEECCCCCCCEEECCCCHHHHCCCCEEEEECCCCCE
VRLGVCGDHQVTNALCAAAVALECGASVEQVAAALTAAPPVSRHRMQVTTRGDGVTVIDD
EEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEC
AYNANPDSMRAGLQALAWIAHQPEATRRSWAVLGEMAELGEDAIAEHDRIGRLAVRLDVS
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCEEEEEEEEE
RLVVVGTGRSISAMHHGAVLEGAWGSGEATADHGADRTAVNVADGDAALALLRAELRPGD
EEEEEECCCCCHHHHCCEEEECCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCCC
VVLVKASNAAGLGAVADALVADDTCGSVRP
EEEEEECCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972