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The map label for this gene is murE

Identifier: 121638040

GI number: 121638040

Start: 2399011

End: 2400618

Strand: Reverse

Name: murE

Synonym: BCG_2175c

Alternate gene names: 121638040

Gene position: 2400618-2399011 (Counterclockwise)

Preceding gene: 121638041

Following gene: 121638039

Centisome position: 54.88

GC content: 73.13

Gene sequence:

>1608_bases
GTGTCATCGCTGGCCCGAGGGATCTCGCGGCGGCGAACGGAGGTGGCGACACAGGTGGAGGCTGCGCCCACTGGCTTGCG
CCCCAACGCCGTCGTGGGCGTTCGGTTGGCCGCACTGGCCGATCAGGTCGGCGCGGCCCTGGCCGAGGGTCCAGCTCAGC
GTGCCGTCACCGAGGACCGGACGGTCACCGGGGTCACGCTGCGCGCCCAGGACGTGTCACCCGGTGACCTGTTCGCCGCC
CTGACCGGCTCGACCACCCACGGGGCCCGCCACGTCGGCGACGCGATCGCACGCGGCGCCGTCGCGGTGCTCACCGACCC
CGCCGGGGTCGCCGAGATCGCCGGACGAGCGGCCGTGCCCGTGTTGGTGCACCCCGCACCCCGCGGCGTGCTCGGCGGCT
TGGCCGCCACCGTGTACGGGCATCCGTCCGAGCGGTTGACGGTTATCGGGATCACCGGAACGTCCGGCAAGACCACCACC
ACCTATCTGGTCGAGGCCGGGTTACGGGCTGCCGGACGCGTCGCCGGGCTGATCGGCACCATCGGCATCCGCGTCGGCGG
CGCCGACCTTCCCAGCGCGCTGACCACCCCGGAGGCCCCCACGCTGCAGGCGATGCTGGCGGCGATGGTCGAACGCGGGG
TGGACACCGTGGTCATGGAGGTGTCCAGCCACGCGCTGGCGCTGGGCCGGGTGGACGGCACCCGGTTCGCCGTCGGCGCC
TTCACCAATCTCTCCCGTGACCACCTGGATTTCCACCCCAGCATGGCCGACTACTTCGAGGCCAAGGCGTCATTGTTCGA
TCCGGACTCGGCACTGCGCGCCCGCACCGCCGTGGTGTGCATCGACGACGACGCCGGGCGCGCGATGGCGGCGCGGGCCG
CCGACGCGATCACCGTCAGCGCCGCCGACCGGCCCGCACACTGGCGCGCCACGGATGTGGCGCCCACGGACGCGGGCGGG
CAACAATTCACCGCCATCGACCCCGCCGGCGTAGGGCATCACATCGGAATCCGGCTACCGGGCCGCTACAACGTCGCCAA
TTGCCTGGTCGCCCTGGCGATTCTGGACACCGTCGGGGTCTCCCCGGAACAGGCGGTGCCGGGCCTGCGTGAGATCCGGG
TCCCGGGGCGGCTCGAGCAGATCGACCGCGGCCAGGGCTTTCTCGCGCTGGTCGACTACGCGCACAAACCGGAAGCGCTG
CGGTCGGTGCTGACCACCTTGGCGCACCCGGACCGCCGGCTGGCGGTGGTGTTCGGCGCCGGCGGCGATCGTGACCCGGG
CAAGCGGGCCCCGATGGGCCGGATAGCCGCGCAGCTGGCCGACTTGGTGGTCGTCACCGACGACAACCCGCGTGACGAAG
ATCCCACGGCGATCCGCCGCGAAATCCTGGCTGGGGCGGCCGAAGTCGGCGGTGATGCCCAGGTCGTCGAGATCGCAGAC
CGGCGGGACGCGATCCGGCACGCGGTCGCCTGGGCGCGCCCCGGCGACGTGGTGCTCATCGCCGGCAAAGGCCACGAGAC
CGGGCAACGCGGCGGCGGGCGGGTCCGCCCGTTCGACGACCGGGTGGAGCTGGCTGCCGCGCTAGAGGCCCTCGAGCGGC
GCGCATGA

Upstream 100 bases:

>100_bases
ACCGCCGCGCGGCGCATCGGCACCTGGATCGGCGCCGCCGCCGAGGGCCAGGTGTCGCGGCAAAACCCGACTGGGTGAGT
GTGCGCGCCCTGTCGGTAGG

Downstream 100 bases:

>100_bases
TCGAGCTGACCGTCGCGCAGATCGCCGAGATCGTCGGGGGCGCAGTGGCCGATATCTCCCCGCAAGACGCCGCGCACCGC
CGCGTCACCGGGACCGTCGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MSSLARGISRRRTEVATQVEAAPTGLRPNAVVGVRLAALADQVGAALAEGPAQRAVTEDRTVTGVTLRAQDVSPGDLFAA
LTGSTTHGARHVGDAIARGAVAVLTDPAGVAEIAGRAAVPVLVHPAPRGVLGGLAATVYGHPSERLTVIGITGTSGKTTT
TYLVEAGLRAAGRVAGLIGTIGIRVGGADLPSALTTPEAPTLQAMLAAMVERGVDTVVMEVSSHALALGRVDGTRFAVGA
FTNLSRDHLDFHPSMADYFEAKASLFDPDSALRARTAVVCIDDDAGRAMAARAADAITVSAADRPAHWRATDVAPTDAGG
QQFTAIDPAGVGHHIGIRLPGRYNVANCLVALAILDTVGVSPEQAVPGLREIRVPGRLEQIDRGQGFLALVDYAHKPEAL
RSVLTTLAHPDRRLAVVFGAGGDRDPGKRAPMGRIAAQLADLVVVTDDNPRDEDPTAIRREILAGAAEVGGDAQVVEIAD
RRDAIRHAVAWARPGDVVLIAGKGHETGQRGGGRVRPFDDRVELAAALEALERRA

Sequences:

>Translated_535_residues
MSSLARGISRRRTEVATQVEAAPTGLRPNAVVGVRLAALADQVGAALAEGPAQRAVTEDRTVTGVTLRAQDVSPGDLFAA
LTGSTTHGARHVGDAIARGAVAVLTDPAGVAEIAGRAAVPVLVHPAPRGVLGGLAATVYGHPSERLTVIGITGTSGKTTT
TYLVEAGLRAAGRVAGLIGTIGIRVGGADLPSALTTPEAPTLQAMLAAMVERGVDTVVMEVSSHALALGRVDGTRFAVGA
FTNLSRDHLDFHPSMADYFEAKASLFDPDSALRARTAVVCIDDDAGRAMAARAADAITVSAADRPAHWRATDVAPTDAGG
QQFTAIDPAGVGHHIGIRLPGRYNVANCLVALAILDTVGVSPEQAVPGLREIRVPGRLEQIDRGQGFLALVDYAHKPEAL
RSVLTTLAHPDRRLAVVFGAGGDRDPGKRAPMGRIAAQLADLVVVTDDNPRDEDPTAIRREILAGAAEVGGDAQVVEIAD
RRDAIRHAVAWARPGDVVLIAGKGHETGQRGGGRVRPFDDRVELAAALEALERRA
>Mature_534_residues
SSLARGISRRRTEVATQVEAAPTGLRPNAVVGVRLAALADQVGAALAEGPAQRAVTEDRTVTGVTLRAQDVSPGDLFAAL
TGSTTHGARHVGDAIARGAVAVLTDPAGVAEIAGRAAVPVLVHPAPRGVLGGLAATVYGHPSERLTVIGITGTSGKTTTT
YLVEAGLRAAGRVAGLIGTIGIRVGGADLPSALTTPEAPTLQAMLAAMVERGVDTVVMEVSSHALALGRVDGTRFAVGAF
TNLSRDHLDFHPSMADYFEAKASLFDPDSALRARTAVVCIDDDAGRAMAARAADAITVSAADRPAHWRATDVAPTDAGGQ
QFTAIDPAGVGHHIGIRLPGRYNVANCLVALAILDTVGVSPEQAVPGLREIRVPGRLEQIDRGQGFLALVDYAHKPEALR
SVLTTLAHPDRRLAVVFGAGGDRDPGKRAPMGRIAAQLADLVVVTDDNPRDEDPTAIRREILAGAAEVGGDAQVVEIADR
RDAIRHAVAWARPGDVVLIAGKGHETGQRGGGRVRPFDDRVELAAALEALERRA

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=481, Percent_Identity=39.2931392931393, Blast_Score=232, Evalue=4e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_MYCBO (P65478)

Other databases:

- EMBL:   BX248341
- RefSeq:   NP_855831.1
- ProteinModelPortal:   P65478
- SMR:   P65478
- EnsemblBacteria:   EBMYCT00000015657
- GeneID:   1091262
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2182c
- GeneTree:   EBGT00050000015779
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- ProtClustDB:   PRK00139
- BioCyc:   MBOV233413:MB2182C-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 55341; Mature: 55210

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: NA

Important sites: BINDING 67-67 BINDING 222-222 BINDING 230-230 BINDING 424-424 BINDING 502-502 BINDING 506-506

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLARGISRRRTEVATQVEAAPTGLRPNAVVGVRLAALADQVGAALAEGPAQRAVTEDR
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCC
TVTGVTLRAQDVSPGDLFAALTGSTTHGARHVGDAIARGAVAVLTDPAGVAEIAGRAAVP
EEEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCHHHHCCCCCCC
VLVHPAPRGVLGGLAATVYGHPSERLTVIGITGTSGKTTTTYLVEAGLRAAGRVAGLIGT
EEECCCCCCHHHHHHHHEECCCCCCEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHH
IGIRVGGADLPSALTTPEAPTLQAMLAAMVERGVDTVVMEVSSHALALGRVDGTRFAVGA
HEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCEEEEEE
FTNLSRDHLDFHPSMADYFEAKASLFDPDSALRARTAVVCIDDDAGRAMAARAADAITVS
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCEEEEEEEECCCCCHHHHHCCCEEEEE
AADRPAHWRATDVAPTDAGGQQFTAIDPAGVGHHIGIRLPGRYNVANCLVALAILDTVGV
CCCCCCCCEECCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
SPEQAVPGLREIRVPGRLEQIDRGQGFLALVDYAHKPEALRSVLTTLAHPDRRLAVVFGA
CHHHHCCCHHEECCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEC
GGDRDPGKRAPMGRIAAQLADLVVVTDDNPRDEDPTAIRREILAGAAEVGGDAQVVEIAD
CCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHH
RRDAIRHAVAWARPGDVVLIAGKGHETGQRGGGRVRPFDDRVELAAALEALERRA
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSLARGISRRRTEVATQVEAAPTGLRPNAVVGVRLAALADQVGAALAEGPAQRAVTEDR
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCC
TVTGVTLRAQDVSPGDLFAALTGSTTHGARHVGDAIARGAVAVLTDPAGVAEIAGRAAVP
EEEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHCCEEEEEECCCCHHHHCCCCCCC
VLVHPAPRGVLGGLAATVYGHPSERLTVIGITGTSGKTTTTYLVEAGLRAAGRVAGLIGT
EEECCCCCCHHHHHHHHEECCCCCCEEEEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHH
IGIRVGGADLPSALTTPEAPTLQAMLAAMVERGVDTVVMEVSSHALALGRVDGTRFAVGA
HEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCEEEEEE
FTNLSRDHLDFHPSMADYFEAKASLFDPDSALRARTAVVCIDDDAGRAMAARAADAITVS
CCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHCCEEEEEEEECCCCCHHHHHCCCEEEEE
AADRPAHWRATDVAPTDAGGQQFTAIDPAGVGHHIGIRLPGRYNVANCLVALAILDTVGV
CCCCCCCCEECCCCCCCCCCCEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCC
SPEQAVPGLREIRVPGRLEQIDRGQGFLALVDYAHKPEALRSVLTTLAHPDRRLAVVFGA
CHHHHCCCHHEECCCCCHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEEC
GGDRDPGKRAPMGRIAAQLADLVVVTDDNPRDEDPTAIRREILAGAAEVGGDAQVVEIAD
CCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEHH
RRDAIRHAVAWARPGDVVLIAGKGHETGQRGGGRVRPFDDRVELAAALEALERRA
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972