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The map label for this gene is cobI

Identifier: 121637951

GI number: 121637951

Start: 2306827

End: 2308353

Strand: Direct

Name: cobI

Synonym: BCG_2085

Alternate gene names: 121637951

Gene position: 2306827-2308353 (Clockwise)

Preceding gene: 121637950

Following gene: 121637954

Centisome position: 52.73

GC content: 70.73

Gene sequence:

>1527_bases
ATGAGCGCTCGGGGCACGCTGTGGGGAGTCGGGCTGGGGCCCGGCGATCCGGAGTTGGTGACCGTCAAGGCCGCCCGGGT
GATTGGCGAGGCCGATGTGGTGGCCTATCACAGCGCCCCACACGGTCACAGCATCGCCCGCGGCATCGCCGAACCGTATC
TGCGGCCCGGTCAGCTCGAGGAGCACCTGGTCTACCCGGTGACCACCGAGGCCACGAATCATCCCGGCGGCTACGCCGGT
GCGCTCGAAGACTTCTACGCCGACGCGACCGAGCGCATCGCCACGCACCTGGACGCCGGGCGCAACGTGGCGCTGCTCGC
CGAAGGCGACCCGTTGTTCTACAGCTCCTACATGCATCTGCACACCCGGCTGACGCGGCGGTTCAACGCCGTCATCGTGC
CCGGTGTGACGTCGGTGAGCGCCGCGTCGGCGGCCGTGGCCACACCGCTGGTGGCCGGCGACCAGGTGTTGTCGGTGCTG
CCGGGCACGCTGCCGGTCGGCGAGCTGACCCGCCGGCTGGCCGACGCCGACGCGGCCGTGGTGGTCAAGCTGGGCCGTTC
GTATCACAATGTGCGGGAGGCGCTTTCGGCGTCCGGCCTACTCGGCGACGCGTTCTACGTGGAGCGGGCCAGCACCGCCG
GCCAACGGGTATTGCCGGCCGCCGACGTCGACGAGACCAGCGTGCCGTACTTCTCGCTGGCCATGTTGCCGGGCGGGCGG
CGTCGTGCGTTGCTGACCGGCACCGTCGCAGTGGTGGGCCTGGGGCCCGGCGACAGCGACTGGATGACACCGCAGAGCCG
GCGTGAGCTGGCCGCCGCGACGGATCTGATCGGCTATCGCGGCTACCTGGACCGGGTCGAAGTCCGCGACGGCCAGCGGC
GCCATCCCAGCGACAACACCGACGAACCCGCCCGGGCGCGGCTGGCCTGCTCGCTGGCCGATCAGGGCCGGGCGGTGGCG
GTGGTGTCCTCCGGCGACCCAGGGGTATTCGCGATGGCCACCGCCGTTTTGGAGGAAGCCGAGCAGTGGCCGGGGGTGCG
GGTCCGGGTGATTCCGGCGATGACCGCCGCCCAGGCCGTCGCCAGCCGGGTCGGCGCGCCGCTGGGACATGACTACGCGG
TGATCTCGTTGTCCGACCGGCTCAAACCCTGGGACGTGATCGCCGCGCGCCTGACCGCCGCGGCCGCCGCCGACCTGGTG
CTGGCCATCTACAACCCGGCTTCGGTGACCCGCACCTGGCAGGTCGGCGCGATGCGCGAGCTGCTGCTGGCCCATCGCGA
CCCTGGCATACCGGTGGTGATCGGCCGCAACGTCTCCGGACCGGTTTCCGGACCGAATGAGGACGTTCGGGTGGTGAAGT
TGGCCGACCTGAACCCCGCCGAAATCGACATGCGCTGCCTATTGATCGTGGGGTCCTCGCAGACCCGGTGGTATTCGGTG
GATTCGCAGGACCGGGTGTTCACCCCGCGCCGCTATCCCGAGGCGGGCAGAGCTACCGCGACAAAGTCGAGCCGCCACAG
CGACTGA

Upstream 100 bases:

>100_bases
TCGAGCGGCCCCGCGGGATGTCCTACCTGGTGGTGCGCGGTCGCCGCGGCGGCAGCGCGATGGCCGCCGCCGCCGTCAAT
GCGATAGCCAGCGACCGCGA

Downstream 100 bases:

>100_bases
AAGAGCTTGCGGCCGAATTCCTCAAGGTCGGCCAGGCTGCCTCCGGAAGGCTCGCCAGTTCGCGCCACGCACCCGGCAAT
CTCCCGAATCGTGCGGCGAC

Product: putative bifunctional protein, CobI-CobJ fusion protein: S-adenosyl-L-methionine-precorrin-2 methyl transferase + precorrin-3 methylase

Products: NA

Alternate protein names: Precorrin-2 C(20)-methyltransferase; S-adenosyl-L-methionine--precorrin-2 methyltransferase; SP2MT; Precorrin-3B C17-methyltransferase; S-adenosyl-L-methionine--precorrin-3B methyltransferase

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MSARGTLWGVGLGPGDPELVTVKAARVIGEADVVAYHSAPHGHSIARGIAEPYLRPGQLEEHLVYPVTTEATNHPGGYAG
ALEDFYADATERIATHLDAGRNVALLAEGDPLFYSSYMHLHTRLTRRFNAVIVPGVTSVSAASAAVATPLVAGDQVLSVL
PGTLPVGELTRRLADADAAVVVKLGRSYHNVREALSASGLLGDAFYVERASTAGQRVLPAADVDETSVPYFSLAMLPGGR
RRALLTGTVAVVGLGPGDSDWMTPQSRRELAAATDLIGYRGYLDRVEVRDGQRRHPSDNTDEPARARLACSLADQGRAVA
VVSSGDPGVFAMATAVLEEAEQWPGVRVRVIPAMTAAQAVASRVGAPLGHDYAVISLSDRLKPWDVIAARLTAAAAADLV
LAIYNPASVTRTWQVGAMRELLLAHRDPGIPVVIGRNVSGPVSGPNEDVRVVKLADLNPAEIDMRCLLIVGSSQTRWYSV
DSQDRVFTPRRYPEAGRATATKSSRHSD

Sequences:

>Translated_508_residues
MSARGTLWGVGLGPGDPELVTVKAARVIGEADVVAYHSAPHGHSIARGIAEPYLRPGQLEEHLVYPVTTEATNHPGGYAG
ALEDFYADATERIATHLDAGRNVALLAEGDPLFYSSYMHLHTRLTRRFNAVIVPGVTSVSAASAAVATPLVAGDQVLSVL
PGTLPVGELTRRLADADAAVVVKLGRSYHNVREALSASGLLGDAFYVERASTAGQRVLPAADVDETSVPYFSLAMLPGGR
RRALLTGTVAVVGLGPGDSDWMTPQSRRELAAATDLIGYRGYLDRVEVRDGQRRHPSDNTDEPARARLACSLADQGRAVA
VVSSGDPGVFAMATAVLEEAEQWPGVRVRVIPAMTAAQAVASRVGAPLGHDYAVISLSDRLKPWDVIAARLTAAAAADLV
LAIYNPASVTRTWQVGAMRELLLAHRDPGIPVVIGRNVSGPVSGPNEDVRVVKLADLNPAEIDMRCLLIVGSSQTRWYSV
DSQDRVFTPRRYPEAGRATATKSSRHSD
>Mature_507_residues
SARGTLWGVGLGPGDPELVTVKAARVIGEADVVAYHSAPHGHSIARGIAEPYLRPGQLEEHLVYPVTTEATNHPGGYAGA
LEDFYADATERIATHLDAGRNVALLAEGDPLFYSSYMHLHTRLTRRFNAVIVPGVTSVSAASAAVATPLVAGDQVLSVLP
GTLPVGELTRRLADADAAVVVKLGRSYHNVREALSASGLLGDAFYVERASTAGQRVLPAADVDETSVPYFSLAMLPGGRR
RALLTGTVAVVGLGPGDSDWMTPQSRRELAAATDLIGYRGYLDRVEVRDGQRRHPSDNTDEPARARLACSLADQGRAVAV
VSSGDPGVFAMATAVLEEAEQWPGVRVRVIPAMTAAQAVASRVGAPLGHDYAVISLSDRLKPWDVIAARLTAAAAADLVL
AIYNPASVTRTWQVGAMRELLLAHRDPGIPVVIGRNVSGPVSGPNEDVRVVKLADLNPAEIDMRCLLIVGSSQTRWYSVD
SQDRVFTPRRYPEAGRATATKSSRHSD

Specific function: Methylates precorrin-2 at the C-20 position to produce precorrin-3A

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBIJ_MYCBO (P66878)

Other databases:

- EMBL:   BX248341
- RefSeq:   NP_855742.1
- ProteinModelPortal:   P66878
- SMR:   P66878
- EnsemblBacteria:   EBMYCT00000015061
- GeneID:   1094149
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2092
- GeneTree:   EBGT00050000016456
- HOGENOM:   HBG480830
- OMA:   KPWEVIA
- ProtClustDB:   CLSK791601
- BioCyc:   MBOV233413:MB2092-MONOMER
- BRENDA:   2.1.1.130
- BRENDA:   2.1.1.131
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR006364
- InterPro:   IPR006363
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- TIGRFAMs:   TIGR01467
- TIGRFAMs:   TIGR01466

Pfam domain/function: PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.130; =2.1.1.131

Molecular weight: Translated: 53912; Mature: 53780

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARGTLWGVGLGPGDPELVTVKAARVIGEADVVAYHSAPHGHSIARGIAEPYLRPGQLE
CCCCCCEEEECCCCCCCCEEEEEHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCC
EHLVYPVTTEATNHPGGYAGALEDFYADATERIATHLDAGRNVALLAEGDPLFYSSYMHL
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHH
HTRLTRRFNAVIVPGVTSVSAASAAVATPLVAGDQVLSVLPGTLPVGELTRRLADADAAV
HHHHHHHCCEEEECCCCCHHHHHHHHHCCHHCCCHHHHHCCCCCCHHHHHHHHHCCCCEE
VVKLGRSYHNVREALSASGLLGDAFYVERASTAGQRVLPAADVDETSVPYFSLAMLPGGR
EEEHHHHHHHHHHHHHHCCCCCCHHEEEHHCCCCCEECCCCCCCCCCCCEEEEEECCCCC
RRALLTGTVAVVGLGPGDSDWMTPQSRRELAAATDLIGYRGYLDRVEVRDGQRRHPSDNT
CEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
DEPARARLACSLADQGRAVAVVSSGDPGVFAMATAVLEEAEQWPGVRVRVIPAMTAAQAV
CCCHHHEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHH
ASRVGAPLGHDYAVISLSDRLKPWDVIAARLTAAAAADLVLAIYNPASVTRTWQVGAMRE
HHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEHHHHHH
LLLAHRDPGIPVVIGRNVSGPVSGPNEDVRVVKLADLNPAEIDMRCLLIVGSSQTRWYSV
HHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEEEC
DSQDRVFTPRRYPEAGRATATKSSRHSD
CCCCCEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SARGTLWGVGLGPGDPELVTVKAARVIGEADVVAYHSAPHGHSIARGIAEPYLRPGQLE
CCCCCEEEECCCCCCCCEEEEEHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCC
EHLVYPVTTEATNHPGGYAGALEDFYADATERIATHLDAGRNVALLAEGDPLFYSSYMHL
CCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEHHHHHHH
HTRLTRRFNAVIVPGVTSVSAASAAVATPLVAGDQVLSVLPGTLPVGELTRRLADADAAV
HHHHHHHCCEEEECCCCCHHHHHHHHHCCHHCCCHHHHHCCCCCCHHHHHHHHHCCCCEE
VVKLGRSYHNVREALSASGLLGDAFYVERASTAGQRVLPAADVDETSVPYFSLAMLPGGR
EEEHHHHHHHHHHHHHHCCCCCCHHEEEHHCCCCCEECCCCCCCCCCCCEEEEEECCCCC
RRALLTGTVAVVGLGPGDSDWMTPQSRRELAAATDLIGYRGYLDRVEVRDGQRRHPSDNT
CEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCC
DEPARARLACSLADQGRAVAVVSSGDPGVFAMATAVLEEAEQWPGVRVRVIPAMTAAQAV
CCCHHHEEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHH
ASRVGAPLGHDYAVISLSDRLKPWDVIAARLTAAAAADLVLAIYNPASVTRTWQVGAMRE
HHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCEEEEHHHHHH
LLLAHRDPGIPVVIGRNVSGPVSGPNEDVRVVKLADLNPAEIDMRCLLIVGSSQTRWYSV
HHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCEEEEC
DSQDRVFTPRRYPEAGRATATKSSRHSD
CCCCCEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972