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The map label for this gene is argF

Identifier: 121637564

GI number: 121637564

Start: 1875189

End: 1876112

Strand: Direct

Name: argF

Synonym: BCG_1695

Alternate gene names: 121637564

Gene position: 1875189-1876112 (Clockwise)

Preceding gene: 121637563

Following gene: 121637565

Centisome position: 42.87

GC content: 68.83

Gene sequence:

>924_bases
GTGATCAGGCATTTCCTGCGCGACGACGATCTGTCCCCGGCCGAACAGGCCGAGGTGCTCGAGCTCGCGGCCGAGCTGAA
GAAAGACCCGGTTAGCCGTCGTCCCCTGCAAGGGCCGCGCGGGGTGGCGGTCATCTTCGACAAGAACTCCACCCGCACCC
GGTTCTCCTTCGAGCTGGGCATCGCGCAGCTGGGCGGGCATGCCGTCGTCGTCGACAGCGGCAGCACCCAGCTGGGCCGC
GACGAAACCCTGCAGGACACCGCAAAGGTGTTGTCCCGCTACGTCGATGCCATCGTCTGGCGAACCTTCGGCCAAGAGCG
GCTGGACGCCATGGCGTCGGTCGCGACGGTGCCCGTGATCAACGCGCTCTCCGATGAGTTCCATCCGTGTCAGGTGTTGG
CCGACCTGCAGACCATCGCCGAACGCAAGGGGGCGCTGCGCGGCCTGAGGTTGTCCTACTTCGGCGACGGCGCCAACAAC
ATGGCCCACTCGCTGCTGCTCGGCGGGGTCACCGCGGGTATCCACGTCACCGTCGCGGCTCCCGAGGGCTTCCTGCCCGA
CCCGTCGGTGCGGGCCGCGGCCGAGCGCCGCGCCCAGGATACCGGCGCCTCGGTGACTGTGACCGCCGACGCCCACGCGG
CCGCCGCCGGCGCCGACGTTCTGGTCACCGACACCTGGACGTCGATGGGCCAGGAAAACGACGGGTTGGACCGAGTGAAG
CCGTTTCGGCCGTTTCAGCTCAACTCGCGACTTCTGGCGCTGGCCGACTCGGATGCCATCGTGTTGCATTGCCTGCCGGC
CCATCGCGGCGACGAGATCACCGACGCGGTGATGGACGGGCCGGCCAGCGCGGTGTGGGACGAGGCCGAAAACCGGCTGC
ACGCGCAGAAGGCGCTGCTGGTGTGGCTGCTGGAGCGCTCATGA

Upstream 100 bases:

>100_bases
ACGTCATCCGGTTGGCGCCGCCGCTGATCATCGCCGAAGCACAGCTCGACGGCTTTGTCGCCGCCTTGCCGGCAATCCTG
GACCGCGCCGTGGGGGCCCC

Downstream 100 bases:

>100_bases
GCCGCGCCAAGGCCGCGCCCGTTGCGGGGCCCGAGGTCGCCGCAAACCGCGCCGGCCGCCAGGCGCGCATCGTGGCGATC
CTGTCGTCGGCGCAGGTGCG

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MIRHFLRDDDLSPAEQAEVLELAAELKKDPVSRRPLQGPRGVAVIFDKNSTRTRFSFELGIAQLGGHAVVVDSGSTQLGR
DETLQDTAKVLSRYVDAIVWRTFGQERLDAMASVATVPVINALSDEFHPCQVLADLQTIAERKGALRGLRLSYFGDGANN
MAHSLLLGGVTAGIHVTVAAPEGFLPDPSVRAAAERRAQDTGASVTVTADAHAAAAGADVLVTDTWTSMGQENDGLDRVK
PFRPFQLNSRLLALADSDAIVLHCLPAHRGDEITDAVMDGPASAVWDEAENRLHAQKALLVWLLERS

Sequences:

>Translated_307_residues
MIRHFLRDDDLSPAEQAEVLELAAELKKDPVSRRPLQGPRGVAVIFDKNSTRTRFSFELGIAQLGGHAVVVDSGSTQLGR
DETLQDTAKVLSRYVDAIVWRTFGQERLDAMASVATVPVINALSDEFHPCQVLADLQTIAERKGALRGLRLSYFGDGANN
MAHSLLLGGVTAGIHVTVAAPEGFLPDPSVRAAAERRAQDTGASVTVTADAHAAAAGADVLVTDTWTSMGQENDGLDRVK
PFRPFQLNSRLLALADSDAIVLHCLPAHRGDEITDAVMDGPASAVWDEAENRLHAQKALLVWLLERS
>Mature_307_residues
MIRHFLRDDDLSPAEQAEVLELAAELKKDPVSRRPLQGPRGVAVIFDKNSTRTRFSFELGIAQLGGHAVVVDSGSTQLGR
DETLQDTAKVLSRYVDAIVWRTFGQERLDAMASVATVPVINALSDEFHPCQVLADLQTIAERKGALRGLRLSYFGDGANN
MAHSLLLGGVTAGIHVTVAAPEGFLPDPSVRAAAERRAQDTGASVTVTADAHAAAAGADVLVTDTWTSMGQENDGLDRVK
PFRPFQLNSRLLALADSDAIVLHCLPAHRGDEITDAVMDGPASAVWDEAENRLHAQKALLVWLLERS

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=305, Percent_Identity=41.3114754098361, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI18105007, Length=311, Percent_Identity=27.3311897106109, Blast_Score=85, Evalue=7e-17,
Organism=Escherichia coli, GI1790703, Length=329, Percent_Identity=37.3860182370821, Blast_Score=221, Evalue=3e-59,
Organism=Escherichia coli, GI1786469, Length=323, Percent_Identity=37.7708978328173, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI2367364, Length=313, Percent_Identity=33.5463258785943, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI48994908, Length=356, Percent_Identity=26.4044943820225, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193204318, Length=312, Percent_Identity=29.8076923076923, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6322373, Length=311, Percent_Identity=35.048231511254, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6322331, Length=308, Percent_Identity=27.5974025974026, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24642586, Length=315, Percent_Identity=28.2539682539683, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_MYCBO (P0A5M9)

Other databases:

- EMBL:   X64203
- EMBL:   BX248339
- PIR:   S26215
- RefSeq:   NP_855336.1
- ProteinModelPortal:   P0A5M9
- SMR:   P0A5M9
- EnsemblBacteria:   EBMYCT00000017319
- GeneID:   1092627
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1684
- GeneTree:   EBGT00050000016463
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- ProtClustDB:   PRK00779
- BioCyc:   MBOV233413:MB1684-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33058; Mature: 33058

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 101-101 BINDING 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRHFLRDDDLSPAEQAEVLELAAELKKDPVSRRPLQGPRGVAVIFDKNSTRTRFSFELG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEC
IAQLGGHAVVVDSGSTQLGRDETLQDTAKVLSRYVDAIVWRTFGQERLDAMASVATVPVI
HHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
NALSDEFHPCQVLADLQTIAERKGALRGLRLSYFGDGANNMAHSLLLGGVTAGIHVTVAA
HHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCEECCEEEEEEC
PEGFLPDPSVRAAAERRAQDTGASVTVTADAHAAAAGADVLVTDTWTSMGQENDGLDRVK
CCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCEEEEECHHHCCCCCCCCCCCC
PFRPFQLNSRLLALADSDAIVLHCLPAHRGDEITDAVMDGPASAVWDEAENRLHAQKALL
CCCCEECCCEEEEEECCCEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VWLLERS
HEEEECC
>Mature Secondary Structure
MIRHFLRDDDLSPAEQAEVLELAAELKKDPVSRRPLQGPRGVAVIFDKNSTRTRFSFELG
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEC
IAQLGGHAVVVDSGSTQLGRDETLQDTAKVLSRYVDAIVWRTFGQERLDAMASVATVPVI
HHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
NALSDEFHPCQVLADLQTIAERKGALRGLRLSYFGDGANNMAHSLLLGGVTAGIHVTVAA
HHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHCCEECCEEEEEEC
PEGFLPDPSVRAAAERRAQDTGASVTVTADAHAAAAGADVLVTDTWTSMGQENDGLDRVK
CCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCHHHCCCCEEEEECHHHCCCCCCCCCCCC
PFRPFQLNSRLLALADSDAIVLHCLPAHRGDEITDAVMDGPASAVWDEAENRLHAQKALL
CCCCEECCCEEEEEECCCEEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
VWLLERS
HEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1406593; 12788972