Click here to switch to the map view.

The map label for this gene is nadB

Identifier: 121637502

GI number: 121637502

Start: 1801052

End: 1802635

Strand: Direct

Name: nadB

Synonym: BCG_1633

Alternate gene names: 121637502

Gene position: 1801052-1802635 (Clockwise)

Preceding gene: 121637501

Following gene: 121637503

Centisome position: 41.17

GC content: 70.33

Gene sequence:

>1584_bases
ATGGCCGGTCCCGCTTGGCGGGATGCGGCCGATGTTGTCGTGATCGGCACGGGCGTTGCCGGGCTGGCGGCGGCATTGGC
CGCCGATCGCGCCGGGCGCAGCGTCGTGGTGCTCAGCAAGGCTGCCCAGACGCACGTGACCGCGACACACTACGCGCAAG
GCGGTATCGCGGTGGTGCTGCCGGACAACGACGACTCGGTCGACGCTCACGTCGCGGACACCTTGGCCGCAGGCGCGGGC
CTATGCGATCCCGATGCGGTGTACTCGATCGTCGCCGACGGCTACCGAGCGGTTACCGATTTGGTCGGAGCTGGGGCACG
GTTGGATGAATCGGTCCCGGGCCGTTGGGCGTTGACGCGCGAAGGCGGGCACTCGCGGCGACGCATCGTGCACGCGGGTG
GCGACGCGACCGGCGCCGAGGTTCAGCGGGCGCTCCAGGATGCCGCCGGGATGCTCGATATCCGCACCGGCCACGTGGCG
TTGCGAGTGCTGCACGACGGTACCGCGGTGACCGGGCTATTAGTGGTCAGACCGGACGGATGCGGCATTATCAGCGCTCC
GTCGGTGATCCTGGCCACCGGCGGGCTCGGGCACCTGTACAGCGCGACCACCAATCCGGCGGGCTCCACCGGCGACGGCA
TCGCCCTGGGATTGTGGGCGGGCGTCGCGGTCAGCGATCTCGAGTTCATCCAGTTCCACCCCACGATGCTTTTTGCCGGA
CGCGCCGGGGGTCGGCGGCCGCTGATCACCGAGGCCATCCGCGGCGAGGGTGCGATCTTGGTGGACAGGCAAGGCAATTC
GATAACGGCAGGCGTGCATCCGATGGGTGATTTGGCGCCGCGCGACGTCGTCGCCGCCGCCATCGACGCGCGGCTGAAGG
CCACCGGCGATCCGTGCGTCTACCTCGACGCCCGCGGCATCGAGGGCTTCGCGTCCCGGTTCCCGACAGTCACGGCATCC
TGCCGGGCTGCCGGCATTGACCCCGTCCGGCAACCGATCCCGGTTGTTCCCGGTGCGCACTACAGCTGCGGCGGCATAGT
GACCGATGTGTACGGCCAGACCGAGCTGCTCGGGTTGTACGCCGCTGGCGAGGTGGCCCGCACCGGGTTGCACGGCGCCA
ACCGCCTGGCCTCCAACAGCTTGCTAGAGGGTTTGGTGGTGGGCGGCCGCGCCGGAAAGGCCGCCGCCGCCCACGCCGCG
GCGGCCGGGCGTTCGCGTGCGACCTCGTCAGCGACCTGGCCCGAACCGATCAGCTACACCGCACTGGACCGCGGCGACCT
GCAACGGGCGATGAGCCGGGACGCGTCGATGTACCGCGCCGCCGCCGGGCTGCACCGGCTGTGCGACAGCCTATCCGGAG
CACAGGTTCGCGACGTGGCTTGTCGCCGCGATTTCGAGGACGTGGCGCTCACGCTGGTCGCGCAGAGCGTGACCGCCGCC
GCCTTGGCCCGCACCGAAAGCCGTGGCTGCCATCATCGCGCGGAGTACCCGTGCACCGTGCCGGAGCAGGCACGCAGCAT
CGTGGTCCGGGGAGCCGACGACGCAAATGCGGTGTGTGTCCAGGCGCTAGTGGCGGTGTGCTGA

Upstream 100 bases:

>100_bases
AGGGTGCCGACGAAGTCCATGTCGATCCGGGAATCGCCGCCAGTGGGCGTCGCAGCGTGCAGCGGATGATCGAAATCGGC
CATCCCGGCGGTGGCGAATG

Downstream 100 bases:

>100_bases
TGGGGTTATCCGACTGGGAGCTGGCTGCGGCTCGAGCAGCAATCGCGCGTGGGCTCGACGAGGACCTCCGGTACGGCCCG
GATGTCACCACATTGGCGAC

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MAGPAWRDAADVVVIGTGVAGLAAALAADRAGRSVVVLSKAAQTHVTATHYAQGGIAVVLPDNDDSVDAHVADTLAAGAG
LCDPDAVYSIVADGYRAVTDLVGAGARLDESVPGRWALTREGGHSRRRIVHAGGDATGAEVQRALQDAAGMLDIRTGHVA
LRVLHDGTAVTGLLVVRPDGCGIISAPSVILATGGLGHLYSATTNPAGSTGDGIALGLWAGVAVSDLEFIQFHPTMLFAG
RAGGRRPLITEAIRGEGAILVDRQGNSITAGVHPMGDLAPRDVVAAAIDARLKATGDPCVYLDARGIEGFASRFPTVTAS
CRAAGIDPVRQPIPVVPGAHYSCGGIVTDVYGQTELLGLYAAGEVARTGLHGANRLASNSLLEGLVVGGRAGKAAAAHAA
AAGRSRATSSATWPEPISYTALDRGDLQRAMSRDASMYRAAAGLHRLCDSLSGAQVRDVACRRDFEDVALTLVAQSVTAA
ALARTESRGCHHRAEYPCTVPEQARSIVVRGADDANAVCVQALVAVC

Sequences:

>Translated_527_residues
MAGPAWRDAADVVVIGTGVAGLAAALAADRAGRSVVVLSKAAQTHVTATHYAQGGIAVVLPDNDDSVDAHVADTLAAGAG
LCDPDAVYSIVADGYRAVTDLVGAGARLDESVPGRWALTREGGHSRRRIVHAGGDATGAEVQRALQDAAGMLDIRTGHVA
LRVLHDGTAVTGLLVVRPDGCGIISAPSVILATGGLGHLYSATTNPAGSTGDGIALGLWAGVAVSDLEFIQFHPTMLFAG
RAGGRRPLITEAIRGEGAILVDRQGNSITAGVHPMGDLAPRDVVAAAIDARLKATGDPCVYLDARGIEGFASRFPTVTAS
CRAAGIDPVRQPIPVVPGAHYSCGGIVTDVYGQTELLGLYAAGEVARTGLHGANRLASNSLLEGLVVGGRAGKAAAAHAA
AAGRSRATSSATWPEPISYTALDRGDLQRAMSRDASMYRAAAGLHRLCDSLSGAQVRDVACRRDFEDVALTLVAQSVTAA
ALARTESRGCHHRAEYPCTVPEQARSIVVRGADDANAVCVQALVAVC
>Mature_526_residues
AGPAWRDAADVVVIGTGVAGLAAALAADRAGRSVVVLSKAAQTHVTATHYAQGGIAVVLPDNDDSVDAHVADTLAAGAGL
CDPDAVYSIVADGYRAVTDLVGAGARLDESVPGRWALTREGGHSRRRIVHAGGDATGAEVQRALQDAAGMLDIRTGHVAL
RVLHDGTAVTGLLVVRPDGCGIISAPSVILATGGLGHLYSATTNPAGSTGDGIALGLWAGVAVSDLEFIQFHPTMLFAGR
AGGRRPLITEAIRGEGAILVDRQGNSITAGVHPMGDLAPRDVVAAAIDARLKATGDPCVYLDARGIEGFASRFPTVTASC
RAAGIDPVRQPIPVVPGAHYSCGGIVTDVYGQTELLGLYAAGEVARTGLHGANRLASNSLLEGLVVGGRAGKAAAAHAAA
AGRSRATSSATWPEPISYTALDRGDLQRAMSRDASMYRAAAGLHRLCDSLSGAQVRDVACRRDFEDVALTLVAQSVTAAA
LARTESRGCHHRAEYPCTVPEQARSIVVRGADDANAVCVQALVAVC

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily

Homologues:

Organism=Homo sapiens, GI156416003, Length=520, Percent_Identity=30.9615384615385, Blast_Score=172, Evalue=7e-43,
Organism=Escherichia coli, GI1788928, Length=550, Percent_Identity=37.4545454545455, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1790597, Length=521, Percent_Identity=33.3973128598848, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1786942, Length=574, Percent_Identity=31.8815331010453, Blast_Score=181, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17505833, Length=543, Percent_Identity=29.6500920810313, Blast_Score=168, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17550100, Length=519, Percent_Identity=29.4797687861272, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI71986328, Length=435, Percent_Identity=28.0459770114943, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6322701, Length=556, Percent_Identity=30.5755395683453, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6322416, Length=556, Percent_Identity=30.5755395683453, Blast_Score=153, Evalue=7e-38,
Organism=Drosophila melanogaster, GI17137288, Length=505, Percent_Identity=29.9009900990099, Blast_Score=155, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24655642, Length=505, Percent_Identity=29.9009900990099, Blast_Score=155, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24655647, Length=505, Percent_Identity=29.9009900990099, Blast_Score=155, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24663005, Length=553, Percent_Identity=28.75226039783, Blast_Score=148, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NADB_MYCBO (P65500)

Other databases:

- EMBL:   BX248339
- RefSeq:   NP_855274.1
- ProteinModelPortal:   P65500
- SMR:   P65500
- EnsemblBacteria:   EBMYCT00000016163
- GeneID:   1092510
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1621
- GeneTree:   EBGT00050000015552
- HOGENOM:   HBG293998
- OMA:   GAYIWNR
- ProtClustDB:   PRK07804
- BioCyc:   MBOV233413:MB1621-MONOMER
- BRENDA:   1.4.3.16
- GO:   GO:0005737
- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288
- Gene3D:   G3DSA:1.20.58.100
- PRINTS:   PR00368
- TIGRFAMs:   TIGR00551

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C; SSF46977 Succ_DH_flav_C

EC number: =1.4.3.16

Molecular weight: Translated: 53785; Mature: 53654

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: ACT_SITE 233-233 ACT_SITE 254-254

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGPAWRDAADVVVIGTGVAGLAAALAADRAGRSVVVLSKAAQTHVTATHYAQGGIAVVL
CCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCHHEEEEEEECCCCEEEEE
PDNDDSVDAHVADTLAAGAGLCDPDAVYSIVADGYRAVTDLVGAGARLDESVPGRWALTR
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEC
EGGHSRRRIVHAGGDATGAEVQRALQDAAGMLDIRTGHVALRVLHDGTAVTGLLVVRPDG
CCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEECCEEEEEEEECCCEEEEEEEECCCC
CGIISAPSVILATGGLGHLYSATTNPAGSTGDGIALGLWAGVAVSDLEFIQFHPTMLFAG
CCEECCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEHHHCCHHCCCCEEEECCEEEEEC
RAGGRRPLITEAIRGEGAILVDRQGNSITAGVHPMGDLAPRDVVAAAIDARLKATGDPCV
CCCCCCCHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHEECCCCCEE
YLDARGIEGFASRFPTVTASCRAAGIDPVRQPIPVVPGAHYSCGGIVTDVYGQTELLGLY
EEECCCCCHHHHHCCCEEECHHHCCCCHHHCCCCCCCCCCCCCCCEEEECCCCCEEEEEE
AAGEVARTGLHGANRLASNSLLEGLVVGGRAGKAAAAHAAAAGRSRATSSATWPEPISYT
EHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEE
ALDRGDLQRAMSRDASMYRAAAGLHRLCDSLSGAQVRDVACRRDFEDVALTLVAQSVTAA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
ALARTESRGCHHRAEYPCTVPEQARSIVVRGADDANAVCVQALVAVC
HHHHHHCCCCCCCCCCCCCCCHHCCEEEEECCCCCHHHHHHHHHHHC
>Mature Secondary Structure 
AGPAWRDAADVVVIGTGVAGLAAALAADRAGRSVVVLSKAAQTHVTATHYAQGGIAVVL
CCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCHHEEEEEEECCCCEEEEE
PDNDDSVDAHVADTLAAGAGLCDPDAVYSIVADGYRAVTDLVGAGARLDESVPGRWALTR
CCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEC
EGGHSRRRIVHAGGDATGAEVQRALQDAAGMLDIRTGHVALRVLHDGTAVTGLLVVRPDG
CCCCCCCEEEECCCCCCHHHHHHHHHHHCCEEEEECCEEEEEEEECCCEEEEEEEECCCC
CGIISAPSVILATGGLGHLYSATTNPAGSTGDGIALGLWAGVAVSDLEFIQFHPTMLFAG
CCEECCCCEEEEECCCCCEEECCCCCCCCCCCCEEEEHHHCCHHCCCCEEEECCEEEEEC
RAGGRRPLITEAIRGEGAILVDRQGNSITAGVHPMGDLAPRDVVAAAIDARLKATGDPCV
CCCCCCCHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHHEECCCCCEE
YLDARGIEGFASRFPTVTASCRAAGIDPVRQPIPVVPGAHYSCGGIVTDVYGQTELLGLY
EEECCCCCHHHHHCCCEEECHHHCCCCHHHCCCCCCCCCCCCCCCEEEECCCCCEEEEEE
AAGEVARTGLHGANRLASNSLLEGLVVGGRAGKAAAAHAAAAGRSRATSSATWPEPISYT
EHHHHHHHCCCHHHHHHHHHHHHHHEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEE
ALDRGDLQRAMSRDASMYRAAAGLHRLCDSLSGAQVRDVACRRDFEDVALTLVAQSVTAA
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
ALARTESRGCHHRAEYPCTVPEQARSIVVRGADDANAVCVQALVAVC
HHHHHHCCCCCCCCCCCCCCCHHCCEEEEECCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972