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The map label for this gene is mutB

Identifier: 121637425

GI number: 121637425

Start: 1709078

End: 1711330

Strand: Direct

Name: mutB

Synonym: BCG_1556

Alternate gene names: 121637425

Gene position: 1709078-1711330 (Clockwise)

Preceding gene: 121637424

Following gene: 121637426

Centisome position: 39.07

GC content: 67.15

Gene sequence:

>2253_bases
ATGACAACCAAGACACCCGTGATCGGCAGCTTCGCCGGCGTTCCGCTGCATAGCGAGCGTGCCGCGCAATCGCCCACAGA
GGCCGCGGTGCACACGCATGTCGCCGCCGCCGCGGCGGCGCACGGGTACACGCCCGAACAGTTGGTGTGGCACACGCCGG
AAGGCATTGACGTCACACCGGTATACATCGCCGCCGACCGGGCCGCCGCCGAAGCCGAGGGCTACCCGCTGCACAGCTTC
CCGGGCGAGCCCCCCTTTGTGCGCGGCCCCTATCCGACGATGTATGTGAACCAGCCGTGGACCATCCGCCAGTACGCCGG
GTTTTCCACCGCCGCGGATTCCAATGCGTTTTACCGACGCAACCTGGCCGCCGGCCAGAAGGGGCTGTCGGTGGCCTTCG
ATCTGGCCACCCACCGCGGCTACGACTCCGACCATCCCCGCGTGCAGGGCGATGTCGGAATGGCCGGTGTGGCAATCGAT
TCCATTCTCGACATGCGACAGCTGTTCGACGGCATCGACCTGTCGACCGTGAGCGTGTCGATGACGATGAACGGTGCGGT
GCTGCCGATCCTGGCGCTGTATGTGGTTGCCGCCGAGGAGCAGGGCGTGGCGCCGGAGCAGCTGGCCGGCACCATCCAGA
ACGACATCCTCAAAGAGTTCATGGTCCGCAACACCTACATCTATCCGCCGAAGCCGTCGATGCGGATCATCTCCGACATC
TTCGCCTACACCAGCGCCAAGATGCCCAAGTTCAACTCCATCTCCATTTCCGGCTATCACATCCAAGAAGCCGGTGCCAC
GGCGGATTTGGAGCTGGCCTACACCCTGGCCGACGGCGTCGACTACATCAGGGCGGGCCTGAACGCCGGCCTGGACATCG
ACAGCTTCGCGCCCCGGCTATCGTTCTTCTGGGGCATCGGGATGAATTTCTTTATGGAGGTCGCCAAACTGCGGGCCGGC
CGGTTGCTGTGGAGCGAGCTGGTCGCACAGTTCGCGCCCAAGAGCGCCAAATCCCTTTCGCTGCGTACACATTCGCAAAC
ATCGGGGTGGTCACTGACCGCCCAGGATGTGTTCAACAACGTGGCGCGCACATGCATCGAGGCGATGGCCGCCACCCAGG
GGCACACCCAGTCGCTGCACACCAACGCCCTGGACGAGGCGCTGGCGCTGCCCACCGATTTTTCGGCCCGCATCGCGCGC
AACACCCAGCTGGTGTTGCAGCAGGAGTCGGGCACCACGCGGCCGATCGACCCGTGGGGGGGCTCCTACTATGTGGAGTG
GCTGACCCATCGGCTCGCGCGGCGAGCCCGGGCGCACATCGCCGAGGTCGCTGAACATGGCGGCATGGCGCAGGCCATCA
GCGACGGCATCCCCAAGCTGCGCATCGAGGAGGCGGCCGCGCGCACCCAGGCCCGCATCGACTCCGGTCAGCAACCGGTG
GTCGGGGTGAACAAATACCAGGTGCCCGAGGACCACGAGATCGAGGTGCTCAAGGTCGAAAACAGCCGGGTGCGCGCCGA
GCAGCTGGCCAAACTGCAGCGGCTGCGGGCAGGCCGGGACGAGCCGGCGGTACGGGCCGCGCTGGCCGAGCTGACCCGCG
CCGCCGCCGAGCAAGGACGCGCCGGAGCAGACGGGCTGGGCAATAATCTGCTGGCCCTGGCCATCGACGCCGCCCGGGCC
CAGGCCACCGTGGGCGAGATCTCCGAAGCGCTGGAGAAGGTGTACGGACGGCACCGGGCCGAGATCCGTACCATTTCCGG
GGTCTACCGCGACGAAGTTGGAAAGGCCCCCAACATCGCAGCCGCAACCGAGCTAGTGGAGAAGTTCGCCGAGGCCGACG
GCCGCCGGCCCAGGATTCTGATCGCCAAGATGGGCCAGGACGGCCACGACCGCGGGCAGAAGGTGATCGCGACCGCGTTC
GCCGACATCGGGTTCGACGTCGACGTGGGGTCGCTGTTTTCCACCCCCGAGGAGGTGGCGCGTCAGGCCGCCGACAACGA
CGTGCACGTGATCGGGGTGTCCTCGCTGGCCGCCGGCCATCTGACGCTGGTGCCGGCGCTGCGCGACGCGTTGGCGCAGG
TGGGCAGGCCCGACATCATGATCGTGGTCGGTGGTGTCATCCCGCCGGGCGACTTCGACGAGCTGTACGCCGCCGGGGCC
ACCGCCATTTTCCCGCCGGGGACGGTGATTGCCGACGCGGCGATTGACCTGCTGCACAGGCTGGCCGAGCGGCTGGGGTA
CACGCTGGATTAG

Upstream 100 bases:

>100_bases
GGCGTTGGGAGATGCCGCACACCGGCCCGACGAGTTTTTGACCGCGAAAATCAATGTGGTGCAAGCCTTGTCGAATCTGC
TGACGCGGTTGGGGGCCTAG

Downstream 100 bases:

>100_bases
CGAGAGGCCCGTGGTGCCGTTTCTGGTTGCATTATCCGGTATCATCTTGGGCGTGCGTGATCATTCGATGACCGTGCGGC
TCGACCAGCAAACTCGCCAG

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM

Number of amino acids: Translated: 750; Mature: 749

Protein sequence:

>750_residues
MTTKTPVIGSFAGVPLHSERAAQSPTEAAVHTHVAAAAAAHGYTPEQLVWHTPEGIDVTPVYIAADRAAAEAEGYPLHSF
PGEPPFVRGPYPTMYVNQPWTIRQYAGFSTAADSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVQGDVGMAGVAID
SILDMRQLFDGIDLSTVSVSMTMNGAVLPILALYVVAAEEQGVAPEQLAGTIQNDILKEFMVRNTYIYPPKPSMRIISDI
FAYTSAKMPKFNSISISGYHIQEAGATADLELAYTLADGVDYIRAGLNAGLDIDSFAPRLSFFWGIGMNFFMEVAKLRAG
RLLWSELVAQFAPKSAKSLSLRTHSQTSGWSLTAQDVFNNVARTCIEAMAATQGHTQSLHTNALDEALALPTDFSARIAR
NTQLVLQQESGTTRPIDPWGGSYYVEWLTHRLARRARAHIAEVAEHGGMAQAISDGIPKLRIEEAAARTQARIDSGQQPV
VGVNKYQVPEDHEIEVLKVENSRVRAEQLAKLQRLRAGRDEPAVRAALAELTRAAAEQGRAGADGLGNNLLALAIDAARA
QATVGEISEALEKVYGRHRAEIRTISGVYRDEVGKAPNIAAATELVEKFAEADGRRPRILIAKMGQDGHDRGQKVIATAF
ADIGFDVDVGSLFSTPEEVARQAADNDVHVIGVSSLAAGHLTLVPALRDALAQVGRPDIMIVVGGVIPPGDFDELYAAGA
TAIFPPGTVIADAAIDLLHRLAERLGYTLD

Sequences:

>Translated_750_residues
MTTKTPVIGSFAGVPLHSERAAQSPTEAAVHTHVAAAAAAHGYTPEQLVWHTPEGIDVTPVYIAADRAAAEAEGYPLHSF
PGEPPFVRGPYPTMYVNQPWTIRQYAGFSTAADSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVQGDVGMAGVAID
SILDMRQLFDGIDLSTVSVSMTMNGAVLPILALYVVAAEEQGVAPEQLAGTIQNDILKEFMVRNTYIYPPKPSMRIISDI
FAYTSAKMPKFNSISISGYHIQEAGATADLELAYTLADGVDYIRAGLNAGLDIDSFAPRLSFFWGIGMNFFMEVAKLRAG
RLLWSELVAQFAPKSAKSLSLRTHSQTSGWSLTAQDVFNNVARTCIEAMAATQGHTQSLHTNALDEALALPTDFSARIAR
NTQLVLQQESGTTRPIDPWGGSYYVEWLTHRLARRARAHIAEVAEHGGMAQAISDGIPKLRIEEAAARTQARIDSGQQPV
VGVNKYQVPEDHEIEVLKVENSRVRAEQLAKLQRLRAGRDEPAVRAALAELTRAAAEQGRAGADGLGNNLLALAIDAARA
QATVGEISEALEKVYGRHRAEIRTISGVYRDEVGKAPNIAAATELVEKFAEADGRRPRILIAKMGQDGHDRGQKVIATAF
ADIGFDVDVGSLFSTPEEVARQAADNDVHVIGVSSLAAGHLTLVPALRDALAQVGRPDIMIVVGGVIPPGDFDELYAAGA
TAIFPPGTVIADAAIDLLHRLAERLGYTLD
>Mature_749_residues
TTKTPVIGSFAGVPLHSERAAQSPTEAAVHTHVAAAAAAHGYTPEQLVWHTPEGIDVTPVYIAADRAAAEAEGYPLHSFP
GEPPFVRGPYPTMYVNQPWTIRQYAGFSTAADSNAFYRRNLAAGQKGLSVAFDLATHRGYDSDHPRVQGDVGMAGVAIDS
ILDMRQLFDGIDLSTVSVSMTMNGAVLPILALYVVAAEEQGVAPEQLAGTIQNDILKEFMVRNTYIYPPKPSMRIISDIF
AYTSAKMPKFNSISISGYHIQEAGATADLELAYTLADGVDYIRAGLNAGLDIDSFAPRLSFFWGIGMNFFMEVAKLRAGR
LLWSELVAQFAPKSAKSLSLRTHSQTSGWSLTAQDVFNNVARTCIEAMAATQGHTQSLHTNALDEALALPTDFSARIARN
TQLVLQQESGTTRPIDPWGGSYYVEWLTHRLARRARAHIAEVAEHGGMAQAISDGIPKLRIEEAAARTQARIDSGQQPVV
GVNKYQVPEDHEIEVLKVENSRVRAEQLAKLQRLRAGRDEPAVRAALAELTRAAAEQGRAGADGLGNNLLALAIDAARAQ
ATVGEISEALEKVYGRHRAEIRTISGVYRDEVGKAPNIAAATELVEKFAEADGRRPRILIAKMGQDGHDRGQKVIATAFA
DIGFDVDVGSLFSTPEEVARQAADNDVHVIGVSSLAAGHLTLVPALRDALAQVGRPDIMIVVGGVIPPGDFDELYAAGAT
AIFPPGTVIADAAIDLLHRLAERLGYTLD

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain

Homologues:

Organism=Homo sapiens, GI156105689, Length=732, Percent_Identity=60.5191256830601, Blast_Score=910, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=704, Percent_Identity=60.3693181818182, Blast_Score=833, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=735, Percent_Identity=61.0884353741497, Blast_Score=920, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MUTB_MYCBO (P65488)

Other databases:

- EMBL:   BX248339
- RefSeq:   NP_855182.1
- ProteinModelPortal:   P65488
- SMR:   P65488
- EnsemblBacteria:   EBMYCT00000016569
- GeneID:   1092387
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1530
- GeneTree:   EBGT00050000017027
- HOGENOM:   HBG454299
- OMA:   RLLWAKL
- ProtClustDB:   PRK09426
- BioCyc:   MBOV233413:MB1530-MONOMER
- BRENDA:   5.4.99.2
- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098
- Gene3D:   G3DSA:3.40.50.280
- Gene3D:   G3DSA:3.20.20.240
- TIGRFAMs:   TIGR00640
- TIGRFAMs:   TIGR00641

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase; SSF52242 Cbl-bd; SSF51703 Cbl-dep_enz_cat

EC number: =5.4.99.2

Molecular weight: Translated: 80605; Mature: 80474

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS51332 B12_BINDING; PS00544 METMALONYL_COA_MUTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKTPVIGSFAGVPLHSERAAQSPTEAAVHTHVAAAAAAHGYTPEQLVWHTPEGIDVTP
CCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCCEEE
VYIAADRAAAEAEGYPLHSFPGEPPFVRGPYPTMYVNQPWTIRQYAGFSTAADSNAFYRR
EEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEHHHCCCCCCCCCCHHHHH
NLAAGQKGLSVAFDLATHRGYDSDHPRVQGDVGMAGVAIDSILDMRQLFDGIDLSTVSVS
HHHCCCCCCEEEEEHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
MTMNGAVLPILALYVVAAEEQGVAPEQLAGTIQNDILKEFMVRNTYIYPPKPSMRIISDI
EEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHH
FAYTSAKMPKFNSISISGYHIQEAGATADLELAYTLADGVDYIRAGLNAGLDIDSFAPRL
HHHHHCCCCCCCEEEEECEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHH
SFFWGIGMNFFMEVAKLRAGRLLWSELVAQFAPKSAKSLSLRTHSQTSGWSLTAQDVFNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEHHHHHHH
VARTCIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLVLQQESGTTRPIDPWG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCC
GSYYVEWLTHRLARRARAHIAEVAEHGGMAQAISDGIPKLRIEEAAARTQARIDSGQQPV
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCE
VGVNKYQVPEDHEIEVLKVENSRVRAEQLAKLQRLRAGRDEPAVRAALAELTRAAAEQGR
ECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
AGADGLGNNLLALAIDAARAQATVGEISEALEKVYGRHRAEIRTISGVYRDEVGKAPNIA
CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AATELVEKFAEADGRRPRILIAKMGQDGHDRGQKVIATAFADIGFDVDVGSLFSTPEEVA
HHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHH
RQAADNDVHVIGVSSLAAGHLTLVPALRDALAQVGRPDIMIVVGGVIPPGDFDELYAAGA
HHHCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHCCC
TAIFPPGTVIADAAIDLLHRLAERLGYTLD
CEECCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TTKTPVIGSFAGVPLHSERAAQSPTEAAVHTHVAAAAAAHGYTPEQLVWHTPEGIDVTP
CCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCCEEE
VYIAADRAAAEAEGYPLHSFPGEPPFVRGPYPTMYVNQPWTIRQYAGFSTAADSNAFYRR
EEEEECCHHHCCCCCCCCCCCCCCCCCCCCCCEEEECCCEEEHHHCCCCCCCCCCHHHHH
NLAAGQKGLSVAFDLATHRGYDSDHPRVQGDVGMAGVAIDSILDMRQLFDGIDLSTVSVS
HHHCCCCCCEEEEEHHHHCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEE
MTMNGAVLPILALYVVAAEEQGVAPEQLAGTIQNDILKEFMVRNTYIYPPKPSMRIISDI
EEECCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHH
FAYTSAKMPKFNSISISGYHIQEAGATADLELAYTLADGVDYIRAGLNAGLDIDSFAPRL
HHHHHCCCCCCCEEEEECEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHHHHH
SFFWGIGMNFFMEVAKLRAGRLLWSELVAQFAPKSAKSLSLRTHSQTSGWSLTAQDVFNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEHHHHHHH
VARTCIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLVLQQESGTTRPIDPWG
HHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCEEEEEECCCCCCCCCCCC
GSYYVEWLTHRLARRARAHIAEVAEHGGMAQAISDGIPKLRIEEAAARTQARIDSGQQPV
CHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEHHHHHHHHHHHHCCCCCCE
VGVNKYQVPEDHEIEVLKVENSRVRAEQLAKLQRLRAGRDEPAVRAALAELTRAAAEQGR
ECCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
AGADGLGNNLLALAIDAARAQATVGEISEALEKVYGRHRAEIRTISGVYRDEVGKAPNIA
CCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
AATELVEKFAEADGRRPRILIAKMGQDGHDRGQKVIATAFADIGFDVDVGSLFSTPEEVA
HHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCHHHHH
RQAADNDVHVIGVSSLAAGHLTLVPALRDALAQVGRPDIMIVVGGVIPPGDFDELYAAGA
HHHCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHCCC
TAIFPPGTVIADAAIDLLHRLAERLGYTLD
CEECCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972