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The map label for this gene is echA12

Identifier: 121637402

GI number: 121637402

Start: 1684835

End: 1685692

Strand: Direct

Name: echA12

Synonym: BCG_1533

Alternate gene names: 121637402

Gene position: 1684835-1685692 (Clockwise)

Preceding gene: 121637401

Following gene: 121637403

Centisome position: 38.51

GC content: 64.8

Gene sequence:

>858_bases
GTGCCCCACCGCTGCGCGGCGCAAGTCGTCGCCGGGTACCGTTCAACGGTGAGTTTGGTCCTCGTCGAACACCCGCGGCC
CGAGATCGCGCAGATTACCCTCAACCGGCCGGAGCGGATGAACTCCATGGCATTCGATGTCATGGTGCCGCTCAAAGAGG
CCTTAGCGCAGGTCAGCTACGACAACTCGGTGCGGGTGGTGGTGCTGACCGGCGCGGGTCGAGGGTTTTCTTCGGGTGCG
GATCACAAGTCGGCGGGGGTGGTGCCGCACGTCGAGAACTTGACTCGGCCCACCTACGCGCTGCGTTCGATGGAGCTCCT
CGATGACGTCATCTTAATGCTGCGACGGCTGCACCAGCCGGTGATCGCCGCGGTCAACGGCCCCGCCATCGGTGGTGGGC
TGTGCCTGGCACTGGCTGCAGACATTCGGGTGGCCTCGAGTAGCGCCTACTTCCGGGCCGCCGGTATCAACAACGGGCTG
ACCGCCAGCGAATTGGGGCTGAGCTACCTGTTGCCCAGGGCCATTGGATCCTCACGTGCGTTCGAGATCATGTTGACCGG
TCGCGACGTCAGCGCCGAGGAAGCCGAGAGGATCGGGCTGGTATCCCGTCAGGTACCCGATGAACAGCTGCTAGATGCCT
GCTACGCGATCGCCGCACGGATGGCGGGATTCTCGCGGCCGGGAATTGAGTTGACCAAACGTACGCTGTGGAGTGGACTG
GACGCCGCCAGTCTGGAGGCGCACATGCAGGCCGAGGGCTTGGGGCAGCTCTTCGTCCGGCTGCTCACCGCCAACTTCGA
AGAAGCGGTTGCCGCACGGGCCGAGCAGCGGGCGCCGGTGTTCACCGATGACACGTAA

Upstream 100 bases:

>100_bases
CCTGGTGCAGCAGCTCAAGGCCTACGAGGTGGAGGCCGGCGAAGCCACCACCCAGAACGGGCGAGCCCAACAAGCCTGAC
CGGGCGCCAGGCGCCCGGCT

Downstream 100 bases:

>100_bases
CAGCGCCCAAGACAACCGACGACCAGGGAGCGAATGTGATCACAGCTACGGACCTCGAGGTCCGCGCTGGCGCGCGCATC
CTGCTCGCACCCGACGGCCC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MPHRCAAQVVAGYRSTVSLVLVEHPRPEIAQITLNRPERMNSMAFDVMVPLKEALAQVSYDNSVRVVVLTGAGRGFSSGA
DHKSAGVVPHVENLTRPTYALRSMELLDDVILMLRRLHQPVIAAVNGPAIGGGLCLALAADIRVASSSAYFRAAGINNGL
TASELGLSYLLPRAIGSSRAFEIMLTGRDVSAEEAERIGLVSRQVPDEQLLDACYAIAARMAGFSRPGIELTKRTLWSGL
DAASLEAHMQAEGLGQLFVRLLTANFEEAVAARAEQRAPVFTDDT

Sequences:

>Translated_285_residues
MPHRCAAQVVAGYRSTVSLVLVEHPRPEIAQITLNRPERMNSMAFDVMVPLKEALAQVSYDNSVRVVVLTGAGRGFSSGA
DHKSAGVVPHVENLTRPTYALRSMELLDDVILMLRRLHQPVIAAVNGPAIGGGLCLALAADIRVASSSAYFRAAGINNGL
TASELGLSYLLPRAIGSSRAFEIMLTGRDVSAEEAERIGLVSRQVPDEQLLDACYAIAARMAGFSRPGIELTKRTLWSGL
DAASLEAHMQAEGLGQLFVRLLTANFEEAVAARAEQRAPVFTDDT
>Mature_284_residues
PHRCAAQVVAGYRSTVSLVLVEHPRPEIAQITLNRPERMNSMAFDVMVPLKEALAQVSYDNSVRVVVLTGAGRGFSSGAD
HKSAGVVPHVENLTRPTYALRSMELLDDVILMLRRLHQPVIAAVNGPAIGGGLCLALAADIRVASSSAYFRAAGINNGLT
ASELGLSYLLPRAIGSSRAFEIMLTGRDVSAEEAERIGLVSRQVPDEQLLDACYAIAARMAGFSRPGIELTKRTLWSGLD
AASLEAHMQAEGLGQLFVRLLTANFEEAVAARAEQRAPVFTDDT

Specific function: Could possibly oxidize fatty acids using specific components

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family

Homologues:

Organism=Homo sapiens, GI194097323, Length=285, Percent_Identity=29.8245614035088, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI70995211, Length=266, Percent_Identity=31.9548872180451, Blast_Score=104, Evalue=8e-23,
Organism=Homo sapiens, GI31542718, Length=269, Percent_Identity=29.7397769516729, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI68989263, Length=186, Percent_Identity=29.5698924731183, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI260274832, Length=215, Percent_Identity=29.3023255813954, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI45643119, Length=215, Percent_Identity=29.3023255813954, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI260275230, Length=215, Percent_Identity=29.3023255813954, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI4757966, Length=176, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538581, Length=176, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI25453481, Length=176, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI51538573, Length=176, Percent_Identity=28.4090909090909, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI221136756, Length=165, Percent_Identity=29.0909090909091, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI221136753, Length=165, Percent_Identity=29.0909090909091, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI4757968, Length=176, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI49355787, Length=176, Percent_Identity=28.4090909090909, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI221307494, Length=165, Percent_Identity=29.0909090909091, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI116235487, Length=163, Percent_Identity=28.8343558282209, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1787660, Length=264, Percent_Identity=34.8484848484849, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=32.0158102766798, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788597, Length=179, Percent_Identity=33.5195530726257, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI221142681, Length=196, Percent_Identity=33.6734693877551, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790281, Length=198, Percent_Identity=30.3030303030303, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788682, Length=210, Percent_Identity=32.3809523809524, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=36.078431372549, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17536985, Length=228, Percent_Identity=31.140350877193, Blast_Score=106, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17554946, Length=281, Percent_Identity=31.3167259786477, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17540714, Length=202, Percent_Identity=34.6534653465347, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=276, Percent_Identity=26.4492753623188, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17560910, Length=203, Percent_Identity=32.512315270936, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=223, Percent_Identity=28.2511210762332, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535521, Length=188, Percent_Identity=27.1276595744681, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=256, Percent_Identity=25.390625, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=289, Percent_Identity=32.1799307958478, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24653477, Length=289, Percent_Identity=32.1799307958478, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19920382, Length=283, Percent_Identity=30.3886925795053, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922422, Length=275, Percent_Identity=27.2727272727273, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24653139, Length=280, Percent_Identity=32.8571428571429, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357171, Length=223, Percent_Identity=31.390134529148, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24654903, Length=171, Percent_Identity=31.5789473684211, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24650670, Length=205, Percent_Identity=33.1707317073171, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ECH12_MYCBO (Q7U004)

Other databases:

- EMBL:   BX248339
- RefSeq:   NP_855159.1
- ProteinModelPortal:   Q7U004
- SMR:   Q7U004
- EnsemblBacteria:   EBMYCT00000017187
- GeneID:   1092364
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1507
- GeneTree:   EBGT00050000015506
- HOGENOM:   HBG748731
- OMA:   LEGHMQA
- ProtClustDB:   PRK05864
- BioCyc:   MBOV233413:MB1507-MONOMER
- BRENDA:   4.2.1.17
- InterPro:   IPR001753
- InterPro:   IPR018376

Pfam domain/function: PF00378 ECH

EC number: =4.2.1.17

Molecular weight: Translated: 30566; Mature: 30434

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHRCAAQVVAGYRSTVSLVLVEHPRPEIAQITLNRPERMNSMAFDVMVPLKEALAQVSY
CCCHHHHHHHHHHHHEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
DNSVRVVVLTGAGRGFSSGADHKSAGVVPHVENLTRPTYALRSMELLDDVILMLRRLHQP
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCH
VIAAVNGPAIGGGLCLALAADIRVASSSAYFRAAGINNGLTASELGLSYLLPRAIGSSRA
HEEECCCCCCCHHHHHHHHHHHHEECCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCE
FEIMLTGRDVSAEEAERIGLVSRQVPDEQLLDACYAIAARMAGFSRPGIELTKRTLWSGL
EEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
DAASLEAHMQAEGLGQLFVRLLTANFEEAVAARAEQRAPVFTDDT
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PHRCAAQVVAGYRSTVSLVLVEHPRPEIAQITLNRPERMNSMAFDVMVPLKEALAQVSY
CCHHHHHHHHHHHHEEEEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
DNSVRVVVLTGAGRGFSSGADHKSAGVVPHVENLTRPTYALRSMELLDDVILMLRRLHQP
CCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCH
VIAAVNGPAIGGGLCLALAADIRVASSSAYFRAAGINNGLTASELGLSYLLPRAIGSSRA
HEEECCCCCCCHHHHHHHHHHHHEECCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCE
FEIMLTGRDVSAEEAERIGLVSRQVPDEQLLDACYAIAARMAGFSRPGIELTKRTLWSGL
EEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
DAASLEAHMQAEGLGQLFVRLLTANFEEAVAARAEQRAPVFTDDT
CHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972