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The map label for this gene is moeY [C]

Identifier: 121637286

GI number: 121637286

Start: 1548399

End: 1550546

Strand: Reverse

Name: moeY [C]

Synonym: BCG_1417c

Alternate gene names: 121637286

Gene position: 1550546-1548399 (Counterclockwise)

Preceding gene: 121637287

Following gene: 121637285

Centisome position: 35.44

GC content: 61.73

Gene sequence:

>2148_bases
GTGACCATCCCACACGAGGGCGGCAGCACCGGCATTCTCGTCCTTCGTGACGATGACCACGACGATGTCCTGGTGCTCGA
TCGACTACGCTCAGATCCGAGCATCGAGTTCGTCGACCGATTCGCAGAACAGCTAGCGGGCGTGCGGCGCCTGCTGCCAC
AGCCTGATCCAGATCTGCTAGAAGAAGCGAAGCGATGGGCCTATTATCCCTGGCGGCGCATGGTTGTTGCCATTCTGGGT
CCCCGGGGGTTCCGTGCCGTGCGCTTGGACCGCAACAGGCACCTCATCACCGCTGAGGAACAACGCGCACTGCACGCGCT
ACGGGTCGGCGTCGTCGGACTCAGCGCCGGCCATGCGATCGCATACACCCTTGCCGCCGAAGGTGCCTGTGGCACACTGC
GGTTGGCCGATTTCGACAAAATTGAGCTGTCGAACCTGAATCGCGTGCCGGTCGGCGTCTTCGACATTGGCCTCAACAAG
GCGATGATTGCGGCGCGCAGGATCGCGGAGCTGGATCCCTACCTGGCCGTGGACCTCGTTACATCCGGGCTCTCGCCGGA
ATCCGTCGACGAGTTCCTCGACGGCCTTGACGTCGTTATCGAAGAATGCGACTCGCTGGATATCAAAGTGATACTGCGAC
AAGCCGCGTGCGCCCGTGGTGTCCCCGTACTGATGGCGACCAGTGACCGCGGCCTGGTCGATGTCGAACGCTACGACGTC
GAACCTGGGCGACCGATCTTTCATGGGCTATTGGGCGACATCGACGCCGACAAGCTGTGTGGACTGACCACAAAAGACAA
AGTCCCCCACGTTCTCAACATCCTTGATTGCCAAGAGCTCTCGGCGCGTTGTGCGGCGTCGATGATCGAAGTTGACCAGA
CGTTGTGGGGATGGCCGCAGTTGGCAGGCGATATCTGGGTGGGCGCAGCGACGGTAGCTGAAGCGGTACGCAGAATCGGT
CTGGGAGAACCACTTGAGTCTGGCCGGGTACGGGTGGATGTGTCCGCAGCGCTAGATCGCCTCGATCAACCGCCCATGCC
GTCTCGCGGGAACGGATGGTTGCTCGAGTCCGTGCCGCCAACAGCGCCAGCCGAGCCGCAGCCAACCAGCGAAATCGTGG
CTCAGGCGGCGATTCGCGCGCCTTCAGGGGGCAACGTTCAGCCATGGCATGTTGTGGCCAAACAGCATTCGCTGACCATC
CGACTAGCACCTGAGCACACCAGCGCAATGGATATCGCGTTTCGTGGTAGCGCAGTGGCCGTCGGCGCGGCCATGTTTAA
CGCGCGTGTCGCTGCCGCTGCACACCGGGTCCTGGGATCAGTAGAGTTCGACGAATCCCAGCCCGACTCGCCGCTGCAAG
CGACAATGCATTTTGGCCGTGGTGATGATCCAAGCCTTGCGGCCCTTTACCGGCCGATGCTGCTCAGAACGACCAATCGC
CACCACGGTATGCCCGGTCACGTCCACCCCGCGACCGTTGAGTTGTTAACGAACACCGCCGCGGCTGAGGGTGCGCGGTT
ACAGCTGTTGCTGTCACGAAACGAGATCGACCGGGCTGCAACAATTTTAGCGGCTGCAGACCGAATCCGCTATCTCACCC
CGAGGCTACATGAGGAAATGATGTCCGAGCTCCGGTGGCCGGGCGACCCTTCGCTCGATGCGGGCATCGATGTCCGAAGC
CTCGAACTGGATTCCGGCGAATTGCGGGTTCTCGATATCCTGCGACGCTCCGACGTCGTGGCACGGCTAGCGCAATGGGA
TTGCGGAACAGCCCTCGAAGACAACACCAATGAGCGCGTGTCGGCGAGTTCAGCGCTCGCAATCGTCTACGTTGATGGCG
CCACGCTCACCGATTTTGCGCGTGGCGGATCAGCCATGCAGGCGGTGTGGATTGTGGCCCAACAACATGGACTGGCCGTA
CAACCGATGTCACCGATCTTCCTGTATGCACGTGGCCGCCACGACCTCGACCAAGCGTCACCCCATTTCGCCGCACAGCT
GCACCGCCTGCAACTCGACTTTCGGGAACTGGTCAAGCCGGGCAAAGAAGGGCACGAGGTGTTGATCTTCAGGTTGTTCC
ACGCGCCACCGCCGTCGGTGTGTAGCCGCCGACGCGTACGGCACGCCATACCAGAGCCGCACAGATAG

Upstream 100 bases:

>100_bases
AGCCAAAACAACTGTCAAGAATGCTTGTCGAGTCGCGCAAGCTTCTGCGCGATGTAGAAGCGTTGAGCGCAACCACCGCA
GCGACCGCTGGAGCCGAACA

Downstream 100 bases:

>100_bases
CATCCGAGATGTGCAACGACACCGCGACGCCGCAGCTTGAGGAGCTCGTCACCACCGTAGCCAACCAGCTCATGACAGTC
GACGCTGCCACGTCAGCCGA

Product: hypothetical protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MTIPHEGGSTGILVLRDDDHDDVLVLDRLRSDPSIEFVDRFAEQLAGVRRLLPQPDPDLLEEAKRWAYYPWRRMVVAILG
PRGFRAVRLDRNRHLITAEEQRALHALRVGVVGLSAGHAIAYTLAAEGACGTLRLADFDKIELSNLNRVPVGVFDIGLNK
AMIAARRIAELDPYLAVDLVTSGLSPESVDEFLDGLDVVIEECDSLDIKVILRQAACARGVPVLMATSDRGLVDVERYDV
EPGRPIFHGLLGDIDADKLCGLTTKDKVPHVLNILDCQELSARCAASMIEVDQTLWGWPQLAGDIWVGAATVAEAVRRIG
LGEPLESGRVRVDVSAALDRLDQPPMPSRGNGWLLESVPPTAPAEPQPTSEIVAQAAIRAPSGGNVQPWHVVAKQHSLTI
RLAPEHTSAMDIAFRGSAVAVGAAMFNARVAAAAHRVLGSVEFDESQPDSPLQATMHFGRGDDPSLAALYRPMLLRTTNR
HHGMPGHVHPATVELLTNTAAAEGARLQLLLSRNEIDRAATILAAADRIRYLTPRLHEEMMSELRWPGDPSLDAGIDVRS
LELDSGELRVLDILRRSDVVARLAQWDCGTALEDNTNERVSASSALAIVYVDGATLTDFARGGSAMQAVWIVAQQHGLAV
QPMSPIFLYARGRHDLDQASPHFAAQLHRLQLDFRELVKPGKEGHEVLIFRLFHAPPPSVCSRRRVRHAIPEPHR

Sequences:

>Translated_715_residues
MTIPHEGGSTGILVLRDDDHDDVLVLDRLRSDPSIEFVDRFAEQLAGVRRLLPQPDPDLLEEAKRWAYYPWRRMVVAILG
PRGFRAVRLDRNRHLITAEEQRALHALRVGVVGLSAGHAIAYTLAAEGACGTLRLADFDKIELSNLNRVPVGVFDIGLNK
AMIAARRIAELDPYLAVDLVTSGLSPESVDEFLDGLDVVIEECDSLDIKVILRQAACARGVPVLMATSDRGLVDVERYDV
EPGRPIFHGLLGDIDADKLCGLTTKDKVPHVLNILDCQELSARCAASMIEVDQTLWGWPQLAGDIWVGAATVAEAVRRIG
LGEPLESGRVRVDVSAALDRLDQPPMPSRGNGWLLESVPPTAPAEPQPTSEIVAQAAIRAPSGGNVQPWHVVAKQHSLTI
RLAPEHTSAMDIAFRGSAVAVGAAMFNARVAAAAHRVLGSVEFDESQPDSPLQATMHFGRGDDPSLAALYRPMLLRTTNR
HHGMPGHVHPATVELLTNTAAAEGARLQLLLSRNEIDRAATILAAADRIRYLTPRLHEEMMSELRWPGDPSLDAGIDVRS
LELDSGELRVLDILRRSDVVARLAQWDCGTALEDNTNERVSASSALAIVYVDGATLTDFARGGSAMQAVWIVAQQHGLAV
QPMSPIFLYARGRHDLDQASPHFAAQLHRLQLDFRELVKPGKEGHEVLIFRLFHAPPPSVCSRRRVRHAIPEPHR
>Mature_714_residues
TIPHEGGSTGILVLRDDDHDDVLVLDRLRSDPSIEFVDRFAEQLAGVRRLLPQPDPDLLEEAKRWAYYPWRRMVVAILGP
RGFRAVRLDRNRHLITAEEQRALHALRVGVVGLSAGHAIAYTLAAEGACGTLRLADFDKIELSNLNRVPVGVFDIGLNKA
MIAARRIAELDPYLAVDLVTSGLSPESVDEFLDGLDVVIEECDSLDIKVILRQAACARGVPVLMATSDRGLVDVERYDVE
PGRPIFHGLLGDIDADKLCGLTTKDKVPHVLNILDCQELSARCAASMIEVDQTLWGWPQLAGDIWVGAATVAEAVRRIGL
GEPLESGRVRVDVSAALDRLDQPPMPSRGNGWLLESVPPTAPAEPQPTSEIVAQAAIRAPSGGNVQPWHVVAKQHSLTIR
LAPEHTSAMDIAFRGSAVAVGAAMFNARVAAAAHRVLGSVEFDESQPDSPLQATMHFGRGDDPSLAALYRPMLLRTTNRH
HGMPGHVHPATVELLTNTAAAEGARLQLLLSRNEIDRAATILAAADRIRYLTPRLHEEMMSELRWPGDPSLDAGIDVRSL
ELDSGELRVLDILRRSDVVARLAQWDCGTALEDNTNERVSASSALAIVYVDGATLTDFARGGSAMQAVWIVAQQHGLAVQ
PMSPIFLYARGRHDLDQASPHFAAQLHRLQLDFRELVKPGKEGHEVLIFRLFHAPPPSVCSRRRVRHAIPEPHR

Specific function: Catalyzes The Adenylation By ATP Of The Carboxyl- Terminal Glycine Of This. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 78166; Mature: 78035

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPHEGGSTGILVLRDDDHDDVLVLDRLRSDPSIEFVDRFAEQLAGVRRLLPQPDPDLL
CCCCCCCCCEEEEEEECCCCCCEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
EEAKRWAYYPWRRMVVAILGPRGFRAVRLDRNRHLITAEEQRALHALRVGVVGLSAGHAI
HHHHHCCCCCHHHHEEHEECCCCCEEEEECCCCEEEECHHHHHHHHHHHHHEEECCCCEE
AYTLAAEGACGTLRLADFDKIELSNLNRVPVGVFDIGLNKAMIAARRIAELDPYLAVDLV
EEEEECCCCCCEEEECCCCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEHHH
TSGLSPESVDEFLDGLDVVIEECDSLDIKVILRQAACARGVPVLMATSDRGLVDVERYDV
HCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECC
EPGRPIFHGLLGDIDADKLCGLTTKDKVPHVLNILDCQELSARCAASMIEVDQTLWGWPQ
CCCCHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LAGDIWVGAATVAEAVRRIGLGEPLESGRVRVDVSAALDRLDQPPMPSRGNGWLLESVPP
HCCHHEECHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCEEECCCCC
TAPAEPQPTSEIVAQAAIRAPSGGNVQPWHVVAKQHSLTIRLAPEHTSAMDIAFRGSAVA
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEECCCCCCEEEEEECCCHHH
VGAAMFNARVAAAAHRVLGSVEFDESQPDSPLQATMHFGRGDDPSLAALYRPMLLRTTNR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCCCCCCHHHHHHHHHHEECCC
HHGMPGHVHPATVELLTNTAAAEGARLQLLLSRNEIDRAATILAAADRIRYLTPRLHEEM
CCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHH
MSELRWPGDPSLDAGIDVRSLELDSGELRVLDILRRSDVVARLAQWDCGTALEDNTNERV
HHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
SASSALAIVYVDGATLTDFARGGSAMQAVWIVAQQHGLAVQPMSPIFLYARGRHDLDQAS
ECCCEEEEEEECCCCHHHHHCCCCHHHEEEEEECCCCCEECCCCCEEEEECCCCCCHHCC
PHFAAQLHRLQLDFRELVKPGKEGHEVLIFRLFHAPPPSVCSRRRVRHAIPEPHR
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TIPHEGGSTGILVLRDDDHDDVLVLDRLRSDPSIEFVDRFAEQLAGVRRLLPQPDPDLL
CCCCCCCCEEEEEEECCCCCCEEEEEHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
EEAKRWAYYPWRRMVVAILGPRGFRAVRLDRNRHLITAEEQRALHALRVGVVGLSAGHAI
HHHHHCCCCCHHHHEEHEECCCCCEEEEECCCCEEEECHHHHHHHHHHHHHEEECCCCEE
AYTLAAEGACGTLRLADFDKIELSNLNRVPVGVFDIGLNKAMIAARRIAELDPYLAVDLV
EEEEECCCCCCEEEECCCCCEECCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEHHH
TSGLSPESVDEFLDGLDVVIEECDSLDIKVILRQAACARGVPVLMATSDRGLVDVERYDV
HCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEEEECC
EPGRPIFHGLLGDIDADKLCGLTTKDKVPHVLNILDCQELSARCAASMIEVDQTLWGWPQ
CCCCHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LAGDIWVGAATVAEAVRRIGLGEPLESGRVRVDVSAALDRLDQPPMPSRGNGWLLESVPP
HCCHHEECHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCEEECCCCC
TAPAEPQPTSEIVAQAAIRAPSGGNVQPWHVVAKQHSLTIRLAPEHTSAMDIAFRGSAVA
CCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEECCCCCCEEEEEECCCHHH
VGAAMFNARVAAAAHRVLGSVEFDESQPDSPLQATMHFGRGDDPSLAALYRPMLLRTTNR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCCCCCCHHHHHHHHHHEECCC
HHGMPGHVHPATVELLTNTAAAEGARLQLLLSRNEIDRAATILAAADRIRYLTPRLHEEM
CCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCHHHHHHH
MSELRWPGDPSLDAGIDVRSLELDSGELRVLDILRRSDVVARLAQWDCGTALEDNTNERV
HHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCE
SASSALAIVYVDGATLTDFARGGSAMQAVWIVAQQHGLAVQPMSPIFLYARGRHDLDQAS
ECCCEEEEEEECCCCHHHHHCCCCHHHEEEEEECCCCCEECCCCCEEEEECCCCCCHHCC
PHFAAQLHRLQLDFRELVKPGKEGHEVLIFRLFHAPPPSVCSRRRVRHAIPEPHR
HHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]