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The map label for this gene is metS

Identifier: 121636936

GI number: 121636936

Start: 1155933

End: 1157492

Strand: Reverse

Name: metS

Synonym: BCG_1064c

Alternate gene names: 121636936

Gene position: 1157492-1155933 (Counterclockwise)

Preceding gene: 121636943

Following gene: 121636934

Centisome position: 26.46

GC content: 64.04

Gene sequence:

>1560_bases
ATGAAGCCCTATTACGTCACCACCGCGATCGCATATCCCAACGCTGCACCCCACGTAGGTCACGCCTACGAATACATCGC
CACCGACGCGATCGCCCGGTTCAAACGGCTGGATGGCTATGACGTGCGCTTCCTGACCGGGACCGACGAGCATGGCCTGA
AGGTCGCACAAGCCGCCGCGGCAGCGGGCGTGCCCACCGCGGCGCTTGCCCGGCGCAATTCCGACGTGTTTCAGCGCATG
CAGGAGGCGCTGAACATCTCCTTCGACCGATTCATCCGCACTACCGATGCCGACCACCACGAGGCGTCCAAGGAACTCTG
GCGACGGATGTCGGCGGCCGGCGACATCTATCTGGACAACTATTCCGGGTGGTACTCGGTGCGCGACGAGCGGTTCTTCG
TCGAATCGGAGACCCAACTTGTCGACGGCACGCGCCTGACGGTAGAGACCGGCACGCCGGTGACCTGGACCGAGGAGCAG
ACCTACTTCTTCCGGCTGTCGGCCTATACCGACAAGCTGCTGGCCCACTATCACGCCAACCCCGACTTCATCGCGCCGGA
GACGCGGCGCAACGAAGTGATCAGCTTCGTCTCCGGCGGCCTGGACGACCTGTCGATCTCGCGCACCTCGTTTGACTGGG
GTGTGCAGGTGCCCGAGCACCCCGACCACGTCATGTACGTCTGGGTCGACGCGCTGACCAATTACCTGACCGGGGCGGGC
TTCCCGGATACCGACTCGGAGTTGTTCCGCCGCTACTGGCCCGCCGATTTGCACATGATCGGCAAGGACATCATCAGGTT
TCATGCCGTCTATTGGCCGGCGTTTTTGATGTCAGCCGGAATCGAGTTGCCGCGAAGGATCTTCGCGCACGGGTTCTTGC
ACAACCGCGGCGAGAAGATGAGTAAATCGGTGGGCAACATCGTCGACCCGGTTGCCCTGGCGGAAGCGCTCGGGGTGGAC
CAGGTCCGCTACTTCCTGTTGCGGGAGGTCCCGTTCGGCCAGGACGGCAGTTACAGCGACGAGGCCATCGTCACTCGGAT
CAACACCGATCTGGCCAACGAGCTCGGCAACTTGGCCCAACGCTCGTTGTCGATGGTGGCCAAAAACCTTGACGGCAGGG
TGCCCAACCCGGGTGAGTTCGCCGACGCCGACGCCGCGTTGCTTGCGACCGCCGATGGCTTGTTGGAGCGAGTGCGCGGT
CACTTCGACGCACAGGCGATGCACCTGGCGCTGGAGGCGATCTGGCTGATGCTCGGCGACGCGAACAAGTACTTTTCGGT
GCAGCAGCCGTGGGTACTGCGCAAGAGCGAGTCCGAAGCCGATCAGGCCCGGTTCCGCACCACGCTCTACGTCACCTGCG
AGGTAGTCCGCATCGCGGCACTGCTGATCCAGCCGGTGATGCCGGAGTCGGCCGGCAAAATTTTGGACCTGCTCGGCCAG
GCCCCAAACCAGCGGTCGTTCGCCGCCGTAGGTGTTCGGCTGACCCCCGGCACAGCGCTGCCGCCGCCCACCGGGGTATT
TCCCCGCTACCAGCCGCCGCAACCACCCGAAGGCAAGTGA

Upstream 100 bases:

>100_bases
GTGGGCGTCGACCAACGGCGCCAGGGGTTCCGGAGCGGGCGGTGGATCTCGTCTGGCTGGGCGGTCGGAGCTCACCCGCA
CACAATAGGGTGGTGTCGAA

Downstream 100 bases:

>100_bases
GCGGACCGCAGCGACGGGAAAGCCACCTACGAAGCGTTGACCGCGGTCTGCGCGTCGCGTGGGATGTCGAGCGTGGCGAC
GGGATAAAACCCGGAATCGT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKPYYVTTAIAYPNAAPHVGHAYEYIATDAIARFKRLDGYDVRFLTGTDEHGLKVAQAAAAAGVPTAALARRNSDVFQRM
QEALNISFDRFIRTTDADHHEASKELWRRMSAAGDIYLDNYSGWYSVRDERFFVESETQLVDGTRLTVETGTPVTWTEEQ
TYFFRLSAYTDKLLAHYHANPDFIAPETRRNEVISFVSGGLDDLSISRTSFDWGVQVPEHPDHVMYVWVDALTNYLTGAG
FPDTDSELFRRYWPADLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNRGEKMSKSVGNIVDPVALAEALGVD
QVRYFLLREVPFGQDGSYSDEAIVTRINTDLANELGNLAQRSLSMVAKNLDGRVPNPGEFADADAALLATADGLLERVRG
HFDAQAMHLALEAIWLMLGDANKYFSVQQPWVLRKSESEADQARFRTTLYVTCEVVRIAALLIQPVMPESAGKILDLLGQ
APNQRSFAAVGVRLTPGTALPPPTGVFPRYQPPQPPEGK

Sequences:

>Translated_519_residues
MKPYYVTTAIAYPNAAPHVGHAYEYIATDAIARFKRLDGYDVRFLTGTDEHGLKVAQAAAAAGVPTAALARRNSDVFQRM
QEALNISFDRFIRTTDADHHEASKELWRRMSAAGDIYLDNYSGWYSVRDERFFVESETQLVDGTRLTVETGTPVTWTEEQ
TYFFRLSAYTDKLLAHYHANPDFIAPETRRNEVISFVSGGLDDLSISRTSFDWGVQVPEHPDHVMYVWVDALTNYLTGAG
FPDTDSELFRRYWPADLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNRGEKMSKSVGNIVDPVALAEALGVD
QVRYFLLREVPFGQDGSYSDEAIVTRINTDLANELGNLAQRSLSMVAKNLDGRVPNPGEFADADAALLATADGLLERVRG
HFDAQAMHLALEAIWLMLGDANKYFSVQQPWVLRKSESEADQARFRTTLYVTCEVVRIAALLIQPVMPESAGKILDLLGQ
APNQRSFAAVGVRLTPGTALPPPTGVFPRYQPPQPPEGK
>Mature_519_residues
MKPYYVTTAIAYPNAAPHVGHAYEYIATDAIARFKRLDGYDVRFLTGTDEHGLKVAQAAAAAGVPTAALARRNSDVFQRM
QEALNISFDRFIRTTDADHHEASKELWRRMSAAGDIYLDNYSGWYSVRDERFFVESETQLVDGTRLTVETGTPVTWTEEQ
TYFFRLSAYTDKLLAHYHANPDFIAPETRRNEVISFVSGGLDDLSISRTSFDWGVQVPEHPDHVMYVWVDALTNYLTGAG
FPDTDSELFRRYWPADLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNRGEKMSKSVGNIVDPVALAEALGVD
QVRYFLLREVPFGQDGSYSDEAIVTRINTDLANELGNLAQRSLSMVAKNLDGRVPNPGEFADADAALLATADGLLERVRG
HFDAQAMHLALEAIWLMLGDANKYFSVQQPWVLRKSESEADQARFRTTLYVTCEVVRIAALLIQPVMPESAGKILDLLGQ
APNQRSFAAVGVRLTPGTALPPPTGVFPRYQPPQPPEGK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily

Homologues:

Organism=Homo sapiens, GI24308436, Length=546, Percent_Identity=38.0952380952381, Blast_Score=348, Evalue=1e-95,
Organism=Homo sapiens, GI14043022, Length=536, Percent_Identity=26.3059701492537, Blast_Score=140, Evalue=4e-33,
Organism=Escherichia coli, GI1788432, Length=502, Percent_Identity=26.0956175298805, Blast_Score=179, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI212645096, Length=518, Percent_Identity=35.9073359073359, Blast_Score=318, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI71992781, Length=412, Percent_Identity=34.2233009708738, Blast_Score=241, Evalue=8e-64,
Organism=Caenorhabditis elegans, GI17540794, Length=556, Percent_Identity=25.3597122302158, Blast_Score=142, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6321610, Length=545, Percent_Identity=34.1284403669725, Blast_Score=291, Evalue=2e-79,
Organism=Saccharomyces cerevisiae, GI6321704, Length=527, Percent_Identity=22.3908918406072, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21358483, Length=520, Percent_Identity=38.0769230769231, Blast_Score=348, Evalue=4e-96,
Organism=Drosophila melanogaster, GI24655311, Length=559, Percent_Identity=25.5813953488372, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_MYCBO (P59952)

Other databases:

- EMBL:   BX248337
- RefSeq:   NP_854691.1
- ProteinModelPortal:   P59952
- SMR:   P59952
- EnsemblBacteria:   EBMYCT00000017046
- GeneID:   1093350
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1034c
- GeneTree:   EBGT00050000015649
- HOGENOM:   HBG721667
- OMA:   APHIGHA
- ProtClustDB:   PRK11893
- BioCyc:   MBOV233413:MB1034C-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_01228
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF09334 tRNA-synt_1g; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 57994; Mature: 57994

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 302-302

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYYVTTAIAYPNAAPHVGHAYEYIATDAIARFKRLDGYDVRFLTGTDEHGLKVAQAAA
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
AAGVPTAALARRNSDVFQRMQEALNISFDRFIRTTDADHHEASKELWRRMSAAGDIYLDN
HCCCCHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEC
YSGWYSVRDERFFVESETQLVDGTRLTVETGTPVTWTEEQTYFFRLSAYTDKLLAHYHAN
CCCEEEECCCEEEEECCCEEECCEEEEEECCCCEEEECCCEEEEEEHHHHHHHHHHHCCC
PDFIAPETRRNEVISFVSGGLDDLSISRTSFDWGVQVPEHPDHVMYVWVDALTNYLTGAG
CCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCC
FPDTDSELFRRYWPADLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNRGEKM
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHH
SKSVGNIVDPVALAEALGVDQVRYFLLREVPFGQDGSYSDEAIVTRINTDLANELGNLAQ
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHH
RSLSMVAKNLDGRVPNPGEFADADAALLATADGLLERVRGHFDAQAMHLALEAIWLMLGD
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
ANKYFSVQQPWVLRKSESEADQARFRTTLYVTCEVVRIAALLIQPVMPESAGKILDLLGQ
CCCCEECCCCEEEECCCCHHHHHHHEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
APNQRSFAAVGVRLTPGTALPPPTGVFPRYQPPQPPEGK
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPYYVTTAIAYPNAAPHVGHAYEYIATDAIARFKRLDGYDVRFLTGTDEHGLKVAQAAA
CCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHH
AAGVPTAALARRNSDVFQRMQEALNISFDRFIRTTDADHHEASKELWRRMSAAGDIYLDN
HCCCCHHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEC
YSGWYSVRDERFFVESETQLVDGTRLTVETGTPVTWTEEQTYFFRLSAYTDKLLAHYHAN
CCCEEEECCCEEEEECCCEEECCEEEEEECCCCEEEECCCEEEEEEHHHHHHHHHHHCCC
PDFIAPETRRNEVISFVSGGLDDLSISRTSFDWGVQVPEHPDHVMYVWVDALTNYLTGAG
CCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHCCCC
FPDTDSELFRRYWPADLHMIGKDIIRFHAVYWPAFLMSAGIELPRRIFAHGFLHNRGEKM
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHH
SKSVGNIVDPVALAEALGVDQVRYFLLREVPFGQDGSYSDEAIVTRINTDLANELGNLAQ
HHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHH
RSLSMVAKNLDGRVPNPGEFADADAALLATADGLLERVRGHFDAQAMHLALEAIWLMLGD
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
ANKYFSVQQPWVLRKSESEADQARFRTTLYVTCEVVRIAALLIQPVMPESAGKILDLLGQ
CCCCEECCCCEEEECCCCHHHHHHHEEEEEEHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
APNQRSFAAVGVRLTPGTALPPPTGVFPRYQPPQPPEGK
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972