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The map label for this gene is pth

Identifier: 121636943

GI number: 121636943

Start: 1163823

End: 1164398

Strand: Reverse

Name: pth

Synonym: BCG_1071c

Alternate gene names: 121636943

Gene position: 1164398-1163823 (Counterclockwise)

Preceding gene: 121636944

Following gene: 121636936

Centisome position: 26.62

GC content: 66.84

Gene sequence:

>576_bases
ATGGCCGAGCCGTTGCTCGTGGTCGGCCTCGGCAACCCTGGAGCCAATTACGCCCGTACCCGGCACAACCTCGGGTTCGT
GGTCGCCGATCTGCTCGCCGCGCGACTAGGCGCGAAGTTCAAGGCACACAAGCGTTCCGGCGCCGAAGTCGCCACTGGTA
GATCAGCCGGGCGCTCCCTGGTTTTGGCCAAGCCGCGCTGCTACATGAACGAGTCCGGCCGCCAGATAGGGCCGCTGGCG
AAGTTCTACTCGGTGGCGCCGGCCAACATCATCGTCATCCACGACGACCTTGACCTCGAGTTCGGCCGCATCCGGCTCAA
GATCGGCGGCGGCGAAGGCGGCCACAACGGGTTGCGCTCGGTGGTGGCCGCGCTGGGTACCAAAGACTTTCAGCGGGTGC
GTATCGGGATCGGCCGCCCGCCCGGCCGCAAGGACCCGGCGGCGTTTGTGTTGGAGAACTTTACCCCGGCCGAGCGCGCC
GAGGTCCCCACGATCTGCGAGCAGGCCGCCGATGCCACCGAGCTGCTTATCGAACAGGGCATGGAGCCCGCACAAAACCG
CGTCCACGCCTGGTAA

Upstream 100 bases:

>100_bases
GCTGGAGGGCGAAGTCGCAGGAGCCGAGGAAGCCGAGGAAGCCGCGGTAGAAGCCGGCGAAGCCGAGGCCGCTGGCGAGT
CCGAGTAGACGGCTTGCGAC

Downstream 100 bases:

>100_bases
CGACCCGCGTGAGCAGACGCAAAATCCCCTCGCAACCGGCGTGTCGAGGGGATTTTGCGTCTGCTCGGCGGCCTTAGGTG
ACCAGGGTGACGGAGTTGGC

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MAEPLLVVGLGNPGANYARTRHNLGFVVADLLAARLGAKFKAHKRSGAEVATGRSAGRSLVLAKPRCYMNESGRQIGPLA
KFYSVAPANIIVIHDDLDLEFGRIRLKIGGGEGGHNGLRSVVAALGTKDFQRVRIGIGRPPGRKDPAAFVLENFTPAERA
EVPTICEQAADATELLIEQGMEPAQNRVHAW

Sequences:

>Translated_191_residues
MAEPLLVVGLGNPGANYARTRHNLGFVVADLLAARLGAKFKAHKRSGAEVATGRSAGRSLVLAKPRCYMNESGRQIGPLA
KFYSVAPANIIVIHDDLDLEFGRIRLKIGGGEGGHNGLRSVVAALGTKDFQRVRIGIGRPPGRKDPAAFVLENFTPAERA
EVPTICEQAADATELLIEQGMEPAQNRVHAW
>Mature_190_residues
AEPLLVVGLGNPGANYARTRHNLGFVVADLLAARLGAKFKAHKRSGAEVATGRSAGRSLVLAKPRCYMNESGRQIGPLAK
FYSVAPANIIVIHDDLDLEFGRIRLKIGGGEGGHNGLRSVVAALGTKDFQRVRIGIGRPPGRKDPAAFVLENFTPAERAE
VPTICEQAADATELLIEQGMEPAQNRVHAW

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=168, Percent_Identity=37.5, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1787455, Length=187, Percent_Identity=39.572192513369, Blast_Score=118, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321983, Length=176, Percent_Identity=35.7954545454545, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_MYCBO (P65866)

Other databases:

- EMBL:   BX248337
- RefSeq:   NP_854698.1
- ProteinModelPortal:   P65866
- SMR:   P65866
- EnsemblBacteria:   EBMYCT00000017639
- GeneID:   1093359
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1042c
- GeneTree:   EBGT00050000017462
- HOGENOM:   HBG610927
- OMA:   NESGRQV
- ProtClustDB:   PRK05426
- BioCyc:   MBOV233413:MB1042C-MONOMER
- BRENDA:   3.1.1.29
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 20456; Mature: 20325

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPLLVVGLGNPGANYARTRHNLGFVVADLLAARLGAKFKAHKRSGAEVATGRSAGRSL
CCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCCCEECCCCCCCEE
VLAKPRCYMNESGRQIGPLAKFYSVAPANIIVIHDDLDLEFGRIRLKIGGGEGGHNGLRS
EEECCCCEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEECCCCCCHHHHHH
VVAALGTKDFQRVRIGIGRPPGRKDPAAFVLENFTPAERAEVPTICEQAADATELLIEQG
HHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCHHHCCCCHHHHHHCHHHHHHHHCC
MEPAQNRVHAW
CCHHHHCCCCC
>Mature Secondary Structure 
AEPLLVVGLGNPGANYARTRHNLGFVVADLLAARLGAKFKAHKRSGAEVATGRSAGRSL
CCCEEEEECCCCCCCHHHHHCCCHHHHHHHHHHHHCCHHHHHCCCCCCEECCCCCCCEE
VLAKPRCYMNESGRQIGPLAKFYSVAPANIIVIHDDLDLEFGRIRLKIGGGEGGHNGLRS
EEECCCCEECCCCCCHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEEECCCCCCHHHHHH
VVAALGTKDFQRVRIGIGRPPGRKDPAAFVLENFTPAERAEVPTICEQAADATELLIEQG
HHHHHCCCCCEEEEEECCCCCCCCCCHHHHCCCCCCHHHCCCCHHHHHHCHHHHHHHHCC
MEPAQNRVHAW
CCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972