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The map label for this gene is xthA [H]

Identifier: 121636341

GI number: 121636341

Start: 546832

End: 547707

Strand: Reverse

Name: xthA [H]

Synonym: BCG_0466c

Alternate gene names: 121636341

Gene position: 547707-546832 (Counterclockwise)

Preceding gene: 121636342

Following gene: 121636340

Centisome position: 12.52

GC content: 65.75

Gene sequence:

>876_bases
ATGCCCGACGGCACAATTGACGGCGGCCACCCCCAACGCCCGGCTTCGCCGCGCTTGCGATCGCCGCTGCTGCGGCTGGC
CACCTGGAATGTGAATTCGATTCGCACCCGGTTGGATCGTGTCCTCGATTGGCTTGGGCGCGCCGATGTCGACGTGTTGG
CCATGCAGGAGACCAAGTGCCCGGACGGCCAATTCCCGGCCTTGCCGCTGTTCGAACTCGGCTATGACGTCGCACACGTC
GGCTTCGATCAGTGGAACGGCGTGGCGATCGCATCCCGCGTCGGCCTCGACGACGTGCGGGTTGGGTTCGACGGCCAGCC
CAGCTGGAGCGGCAAGCCGGAAGTGGCCGCCACGACGGAGGCCCGTGCCCTGGGCGCCACCTGCGGCGGCATCCGGGTGT
GGAGCCTCTACGTGCCCAACGGGCGCGCCCTGGACGATCCGCACTACACCTACAAGTTGGATTGGCTTGCCGCGCTGCGT
GATACGGCCGAAGGCTGGCTACGTGACGATCCCGCCGCACCAATCGCACTGATGGGCGACTGGAACATCGCTCCGACCGA
TGATGATGTCTGGAGCACCGAATTCTTCGCCGGCTGCACGCACGTTTCCGAACCCGAGCGCAAGGCGTTCAACGCCATTG
TCGACGCGCAATTCACCGATGTAGTACGGCCTTTCACCCCGGGACCCGGGGTTTACACCTACTGGGATTACACGCAGCTG
CGGTTCCCGAAGAAACAGGGCATGCGCATCGACTTCATCCTCGGCTCGCCGGCGTTGGCCGCCCGGGTGATGGACGCCCA
GATCGTACGCGAGGAGCGTAAAGGCAAAGCGCCCAGCGACCACGCTCCGGTGCTCGTCGACCTGCACGCCGGGTAG

Upstream 100 bases:

>100_bases
ACACCGCGACATCCGTCCTCGCCGAGTCCCTGGGCTTCCGGCTGCACCATCACTGCCGCTATCTTCCTGCCCAGTCGGTC
GGCTGGGATACCTTCTAGCC

Downstream 100 bases:

>100_bases
GCGGCGTACGGCGGTAGTCGGCAGTCGCGCCGCACGCGGCAGTTGCGCGTTGTCTCAGTCAGGGCGGCGTGGCAGGCTTG
CATTTCGAGAGCGCTGAACA

Product: putative exodeoxyribonuclease III protein xthA

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 291; Mature: 290

Protein sequence:

>291_residues
MPDGTIDGGHPQRPASPRLRSPLLRLATWNVNSIRTRLDRVLDWLGRADVDVLAMQETKCPDGQFPALPLFELGYDVAHV
GFDQWNGVAIASRVGLDDVRVGFDGQPSWSGKPEVAATTEARALGATCGGIRVWSLYVPNGRALDDPHYTYKLDWLAALR
DTAEGWLRDDPAAPIALMGDWNIAPTDDDVWSTEFFAGCTHVSEPERKAFNAIVDAQFTDVVRPFTPGPGVYTYWDYTQL
RFPKKQGMRIDFILGSPALAARVMDAQIVREERKGKAPSDHAPVLVDLHAG

Sequences:

>Translated_291_residues
MPDGTIDGGHPQRPASPRLRSPLLRLATWNVNSIRTRLDRVLDWLGRADVDVLAMQETKCPDGQFPALPLFELGYDVAHV
GFDQWNGVAIASRVGLDDVRVGFDGQPSWSGKPEVAATTEARALGATCGGIRVWSLYVPNGRALDDPHYTYKLDWLAALR
DTAEGWLRDDPAAPIALMGDWNIAPTDDDVWSTEFFAGCTHVSEPERKAFNAIVDAQFTDVVRPFTPGPGVYTYWDYTQL
RFPKKQGMRIDFILGSPALAARVMDAQIVREERKGKAPSDHAPVLVDLHAG
>Mature_290_residues
PDGTIDGGHPQRPASPRLRSPLLRLATWNVNSIRTRLDRVLDWLGRADVDVLAMQETKCPDGQFPALPLFELGYDVAHVG
FDQWNGVAIASRVGLDDVRVGFDGQPSWSGKPEVAATTEARALGATCGGIRVWSLYVPNGRALDDPHYTYKLDWLAALRD
TAEGWLRDDPAAPIALMGDWNIAPTDDDVWSTEFFAGCTHVSEPERKAFNAIVDAQFTDVVRPFTPGPGVYTYWDYTQLR
FPKKQGMRIDFILGSPALAARVMDAQIVREERKGKAPSDHAPVLVDLHAG

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=286, Percent_Identity=27.6223776223776, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI18375503, Length=286, Percent_Identity=27.6223776223776, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI18375501, Length=286, Percent_Identity=27.6223776223776, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788046, Length=270, Percent_Identity=28.8888888888889, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 32108; Mature: 31977

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDGTIDGGHPQRPASPRLRSPLLRLATWNVNSIRTRLDRVLDWLGRADVDVLAMQETKC
CCCCCCCCCCCCCCCCCCHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
PDGQFPALPLFELGYDVAHVGFDQWNGVAIASRVGLDDVRVGFDGQPSWSGKPEVAATTE
CCCCCCCCCHHHHCCCCEECCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCEEECCH
ARALGATCGGIRVWSLYVPNGRALDDPHYTYKLDWLAALRDTAEGWLRDDPAAPIALMGD
HHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEC
WNIAPTDDDVWSTEFFAGCTHVSEPERKAFNAIVDAQFTDVVRPFTPGPGVYTYWDYTQL
CCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEECCCEE
RFPKKQGMRIDFILGSPALAARVMDAQIVREERKGKAPSDHAPVLVDLHAG
ECCHHHCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC
>Mature Secondary Structure 
PDGTIDGGHPQRPASPRLRSPLLRLATWNVNSIRTRLDRVLDWLGRADVDVLAMQETKC
CCCCCCCCCCCCCCCCCHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
PDGQFPALPLFELGYDVAHVGFDQWNGVAIASRVGLDDVRVGFDGQPSWSGKPEVAATTE
CCCCCCCCCHHHHCCCCEECCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCEEECCH
ARALGATCGGIRVWSLYVPNGRALDDPHYTYKLDWLAALRDTAEGWLRDDPAAPIALMGD
HHHHCCCCCCEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEC
WNIAPTDDDVWSTEFFAGCTHVSEPERKAFNAIVDAQFTDVVRPFTPGPGVYTYWDYTQL
CCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEECCCEE
RFPKKQGMRIDFILGSPALAARVMDAQIVREERKGKAPSDHAPVLVDLHAG
ECCHHHCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]