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The map label for this gene is lgrC [H]

Identifier: 120612852

GI number: 120612852

Start: 4672599

End: 4674305

Strand: Direct

Name: lgrC [H]

Synonym: Aave_4216

Alternate gene names: 120612852

Gene position: 4672599-4674305 (Clockwise)

Preceding gene: 120612851

Following gene: 120612853

Centisome position: 87.29

GC content: 63.97

Gene sequence:

>1707_bases
ATGACCAATGTGATGCACCGGCTTTCGGAAATCGTGCGTCGGCAGCCTCGAGCTGCAGCGCTCATCTCCGATGCACAAAC
GGTCAGCTACCAGGACCTGGGCGTGCGCATTGCAGAAATCGCCCGATCGATCGACATCTGGTTCCTGACGCACCTCGGCC
GCGAAGCGGGCAGCAGCGACGCCATCGGCATCTGCATGGGAAAAAGCACCGATCTCTATGCGTCGATCCTGGCCATCCTC
GCCACAGGCGCGAGCTATGTTCCTGTCGATCCCTTGCTCCCTGCGCAATTGCAGGCGCACATCCTGGAGACCTGCAGATG
CAGGCTGGTTCTGGCGGCGCCCGACACCCGGCTCCCGGTCTCCGGGGTATGCGTTGCCCCGCCTGGCGAAGCCGCCGACA
GCCATCCATCGCAGTCCGCAGCGTGGGCATTCCCATGTCGTGCAACGGGGCAGGACCGCTGCTACACCATCTTCACGTCC
GGATCCACCGGGCGCCCGAAAGGCGTGCAAATCCGGCACGACGGCGTACTGCACCTCGTCGAATGGATGCTCCGCGAGAT
AGCGCTGAAGGAATCCCATCGGGTACTGCAGTACTCCACGATCAACTTCGACGCCTCGGTACTGGACATCTTCCCTGCGC
TCCTGGCAGGTGCCACCCTCTGCATTCCCCGCGACGACCAGCGGCTGTCCGCCACCGGGCTCGCGGAGTTCTGCGCGCGC
CACAGGATCCATCAGGCGTTCCTGCCGCCGGCCATGCTCTCCGTGCTCGACCCGCAGCAGTTCCCGACCCTGGAGACCCT
GCTCACGGGCGGAGAAGCCTGCAGTCCTGCGGTAATCCAGGCGTGGGCGGCGGAGCGGCGGTTCTACAACCTCTACGGCC
CCACGGAGTGCACTGTCCTCGTGGCGTTCAAGCGCATGGAGGCATGTCAGGCGCGGACCAACATCGGGCAGGCTATCGAC
GGCGCACGGTTGCATGTCCTGGATGAACAGATGCAGCCGGCAGTTCGGGGAGAACTGCACGTCGCCGGCCCGATGGTGTC
ACAGGGGTACATGGGCGACTCTCTGGCGACCACGCGGAAGTTCGTCCTTTGCCCCGAAGTGGATGAAGGCCGCCTCTACA
AAACCGGAGATATCGTCGAACGGGATGCCTGCGGCGATCTTCATTTCCTGGGACGTCTGGACCGGCAGGTGAAGGTCCGC
GGATTTCGCGTGGAACTCGAGGAAGTCGAAGGCGTCCTGGTGCAGTCGGGGTGTCTGCAGGCGGCGGTCAGGCTGTCGCC
GGACGGACAGCTGGTCGCCTATGTCGTGCTGCCGCCGCAGATAGGGCTGGACGCACTGCGCCAGCAGCTTGCCCAGCATT
TGAGCGACTACAAGGTCCCGCAGTGCTTCATACCGATACAGCAGCTTCCCCTCAAGGCCAGCGGCAAAGTAGATTTCGAT
GCCCTGCCAGCCACCACGCCACGGCTTGCGGCGGGTGCTTCCAGCCGGCCCTGCGAACCCATCCTGTCCCTCTGGGAGGA
AATCCTGGATCTCCCGCAGGGCTCGCTGGATGCCCATTCCGACTTCCAGGAGGCCGGCGGGACCTCCATCAAGGCGATCC
GCCTGCTGAGCGCCATCGAGGAACGCTTCGGAGTGCGCATCCGGTTCTCGGAGTTCCTCGATAACCCCACGCCCCATTTT
CTGTACAACGCGATACCTCATCCATGA

Upstream 100 bases:

>100_bases
CAACGGTCGGCACAGGCCAGAAAACCATGCTTTTCAGTGCCGGCCCGTTCCAGTGGGGCGCCTGCGTCCTCGAAAGAACC
AACGTTTTCGGGGAGGGTGC

Downstream 100 bases:

>100_bases
CAACGCCCCTGCTCCCCCAGCTGAACCACATTGGCCTGACAGTCCACAGCATCGCGGACACGATTGCCTTCTATCGACAG
ATCACCACGGTCGAAATCTA

Product: amino acid adenylation domain-containing protein

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: ATP-dependent valine adenylase; ValA; Valine activase; ATP-dependent D-valine adenylase; D-ValA; D-valine activase; Valine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan adenylase; TrpA; Tryptophan activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing]; ATP-dependent tryptophan/phenylalanine/tyrosine adenylase; Trp/Phe/TyrA; Tryptophan/phenylalanine/tyrosine activase; ATP-dependent D-leucine adenylase; D-LeuA; D-leucine activase; Leucine racemase [ATP-hydrolyzing] [H]

Number of amino acids: Translated: 568; Mature: 567

Protein sequence:

>568_residues
MTNVMHRLSEIVRRQPRAAALISDAQTVSYQDLGVRIAEIARSIDIWFLTHLGREAGSSDAIGICMGKSTDLYASILAIL
ATGASYVPVDPLLPAQLQAHILETCRCRLVLAAPDTRLPVSGVCVAPPGEAADSHPSQSAAWAFPCRATGQDRCYTIFTS
GSTGRPKGVQIRHDGVLHLVEWMLREIALKESHRVLQYSTINFDASVLDIFPALLAGATLCIPRDDQRLSATGLAEFCAR
HRIHQAFLPPAMLSVLDPQQFPTLETLLTGGEACSPAVIQAWAAERRFYNLYGPTECTVLVAFKRMEACQARTNIGQAID
GARLHVLDEQMQPAVRGELHVAGPMVSQGYMGDSLATTRKFVLCPEVDEGRLYKTGDIVERDACGDLHFLGRLDRQVKVR
GFRVELEEVEGVLVQSGCLQAAVRLSPDGQLVAYVVLPPQIGLDALRQQLAQHLSDYKVPQCFIPIQQLPLKASGKVDFD
ALPATTPRLAAGASSRPCEPILSLWEEILDLPQGSLDAHSDFQEAGGTSIKAIRLLSAIEERFGVRIRFSEFLDNPTPHF
LYNAIPHP

Sequences:

>Translated_568_residues
MTNVMHRLSEIVRRQPRAAALISDAQTVSYQDLGVRIAEIARSIDIWFLTHLGREAGSSDAIGICMGKSTDLYASILAIL
ATGASYVPVDPLLPAQLQAHILETCRCRLVLAAPDTRLPVSGVCVAPPGEAADSHPSQSAAWAFPCRATGQDRCYTIFTS
GSTGRPKGVQIRHDGVLHLVEWMLREIALKESHRVLQYSTINFDASVLDIFPALLAGATLCIPRDDQRLSATGLAEFCAR
HRIHQAFLPPAMLSVLDPQQFPTLETLLTGGEACSPAVIQAWAAERRFYNLYGPTECTVLVAFKRMEACQARTNIGQAID
GARLHVLDEQMQPAVRGELHVAGPMVSQGYMGDSLATTRKFVLCPEVDEGRLYKTGDIVERDACGDLHFLGRLDRQVKVR
GFRVELEEVEGVLVQSGCLQAAVRLSPDGQLVAYVVLPPQIGLDALRQQLAQHLSDYKVPQCFIPIQQLPLKASGKVDFD
ALPATTPRLAAGASSRPCEPILSLWEEILDLPQGSLDAHSDFQEAGGTSIKAIRLLSAIEERFGVRIRFSEFLDNPTPHF
LYNAIPHP
>Mature_567_residues
TNVMHRLSEIVRRQPRAAALISDAQTVSYQDLGVRIAEIARSIDIWFLTHLGREAGSSDAIGICMGKSTDLYASILAILA
TGASYVPVDPLLPAQLQAHILETCRCRLVLAAPDTRLPVSGVCVAPPGEAADSHPSQSAAWAFPCRATGQDRCYTIFTSG
STGRPKGVQIRHDGVLHLVEWMLREIALKESHRVLQYSTINFDASVLDIFPALLAGATLCIPRDDQRLSATGLAEFCARH
RIHQAFLPPAMLSVLDPQQFPTLETLLTGGEACSPAVIQAWAAERRFYNLYGPTECTVLVAFKRMEACQARTNIGQAIDG
ARLHVLDEQMQPAVRGELHVAGPMVSQGYMGDSLATTRKFVLCPEVDEGRLYKTGDIVERDACGDLHFLGRLDRQVKVRG
FRVELEEVEGVLVQSGCLQAAVRLSPDGQLVAYVVLPPQIGLDALRQQLAQHLSDYKVPQCFIPIQQLPLKASGKVDFDA
LPATTPRLAAGASSRPCEPILSLWEEILDLPQGSLDAHSDFQEAGGTSIKAIRLLSAIEERFGVRIRFSEFLDNPTPHFL
YNAIPHP

Specific function: Activates the 7th to 12th amino acids (Val, D-Val, Trp, D-Leu, Xaa and D-Leu) in linear gramicidin and catalyzes the formation of the peptide bond between them. This enzyme is also responsible for the epimerization of the 8th (D-Val), the 10th (D- Leu) an

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 6 acyl carrier domains [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=427, Percent_Identity=26.9320843091335, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI38505220, Length=369, Percent_Identity=26.8292682926829, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI122937307, Length=473, Percent_Identity=23.8900634249471, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI115511026, Length=366, Percent_Identity=27.8688524590164, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1786801, Length=593, Percent_Identity=29.3423271500843, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789201, Length=377, Percent_Identity=26.2599469496021, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1788107, Length=355, Percent_Identity=25.0704225352113, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17550940, Length=561, Percent_Identity=26.3814616755793, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17556356, Length=542, Percent_Identity=25.6457564575646, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI32564420, Length=380, Percent_Identity=26.3157894736842, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI32564422, Length=380, Percent_Identity=26.3157894736842, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17538037, Length=359, Percent_Identity=24.2339832869081, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319591, Length=652, Percent_Identity=23.159509202454, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24648676, Length=558, Percent_Identity=27.0609318996416, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI21355181, Length=478, Percent_Identity=22.5941422594142, Blast_Score=76, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 62061; Mature: 61930

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50075 ACP_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNVMHRLSEIVRRQPRAAALISDAQTVSYQDLGVRIAEIARSIDIWFLTHLGREAGSSD
CCHHHHHHHHHHHCCCCHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AIGICMGKSTDLYASILAILATGASYVPVDPLLPAQLQAHILETCRCRLVLAAPDTRLPV
CEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCC
SGVCVAPPGEAADSHPSQSAAWAFPCRATGQDRCYTIFTSGSTGRPKGVQIRHDGVLHLV
CCEEECCCCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCEEEECCCHHHHH
EWMLREIALKESHRVLQYSTINFDASVLDIFPALLAGATLCIPRDDQRLSATGLAEFCAR
HHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHH
HRIHQAFLPPAMLSVLDPQQFPTLETLLTGGEACSPAVIQAWAAERRFYNLYGPTECTVL
HHHHHHHCCHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHEEECCCCCCEEEH
VAFKRMEACQARTNIGQAIDGARLHVLDEQMQPAVRGELHVAGPMVSQGYMGDSLATTRK
HHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCHHHCCCEEECCCCHHCCCCCCCHHHCCE
FVLCPEVDEGRLYKTGDIVERDACGDLHFLGRLDRQVKVRGFRVELEEVEGVLVQSGCLQ
EEECCCCCCCCEEECCCCCCCCCCCCHHHHHHCCCEEEEEEEEEEHHHHCCHHHHHHHHH
AAVRLSPDGQLVAYVVLPPQIGLDALRQQLAQHLSDYKVPQCFIPIQQLPLKASGKVDFD
HHEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCCCCC
ALPATTPRLAAGASSRPCEPILSLWEEILDLPQGSLDAHSDFQEAGGTSIKAIRLLSAIE
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHH
ERFGVRIRFSEFLDNPTPHFLYNAIPHP
HHHCCEEEEHHHHCCCCCHHCCCCCCCC
>Mature Secondary Structure 
TNVMHRLSEIVRRQPRAAALISDAQTVSYQDLGVRIAEIARSIDIWFLTHLGREAGSSD
CHHHHHHHHHHHCCCCHHHHHHCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AIGICMGKSTDLYASILAILATGASYVPVDPLLPAQLQAHILETCRCRLVLAAPDTRLPV
CEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCC
SGVCVAPPGEAADSHPSQSAAWAFPCRATGQDRCYTIFTSGSTGRPKGVQIRHDGVLHLV
CCEEECCCCCCCCCCCCCCCCEECCCCCCCCCCEEEEEECCCCCCCCCEEEECCCHHHHH
EWMLREIALKESHRVLQYSTINFDASVLDIFPALLAGATLCIPRDDQRLSATGLAEFCAR
HHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHH
HRIHQAFLPPAMLSVLDPQQFPTLETLLTGGEACSPAVIQAWAAERRFYNLYGPTECTVL
HHHHHHHCCHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHEEECCCCCCEEEH
VAFKRMEACQARTNIGQAIDGARLHVLDEQMQPAVRGELHVAGPMVSQGYMGDSLATTRK
HHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCHHHCCCEEECCCCHHCCCCCCCHHHCCE
FVLCPEVDEGRLYKTGDIVERDACGDLHFLGRLDRQVKVRGFRVELEEVEGVLVQSGCLQ
EEECCCCCCCCEEECCCCCCCCCCCCHHHHHHCCCEEEEEEEEEEHHHHCCHHHHHHHHH
AAVRLSPDGQLVAYVVLPPQIGLDALRQQLAQHLSDYKVPQCFIPIQQLPLKASGKVDFD
HHEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCHHHCCCCCCCCCCCC
ALPATTPRLAAGASSRPCEPILSLWEEILDLPQGSLDAHSDFQEAGGTSIKAIRLLSAIE
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCHHHHHHHHHHHH
ERFGVRIRFSEFLDNPTPHFLYNAIPHP
HHHCCEEEEHHHHCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA