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The map label for this gene is rfbB [H]

Identifier: 120612803

GI number: 120612803

Start: 4619004

End: 4620089

Strand: Reverse

Name: rfbB [H]

Synonym: Aave_4166

Alternate gene names: 120612803

Gene position: 4620089-4619004 (Counterclockwise)

Preceding gene: 120612806

Following gene: 120612802

Centisome position: 86.31

GC content: 66.48

Gene sequence:

>1086_bases
ATGATTCTGGTCACCGGCGGCGCGGGTTTCATCGGCGCCAACTTCGTCCTCGACTGGCTGGCCTGCGGCGATGAGCCCGT
CGTCAACCTCGACAAGCTCACCTACGCAGGCAATCTGCAGAACCTCGCTTCGCTGCAAGGCGATGCGCGCCACCTGTTCG
TCCAGGGCGACATCGGCGACCGGGAGCTGCTCACCCGGCTGCTGGCCGGGCACCGGCCGCGGGCCGTCGTGAATTTCGCG
GCCGAATCGCATGTGGACCGTTCCATCCATGGCCCGGAAGACTTCATCCAGACCAACGTGGTCGGCACCTTCCGCCTGCT
CGAAGCCGTCCGCGAGTACTGGCAGTCCCTGCCCGGGGACGAGCGCGCCGCGTTCCGGTTCCTGCACGTCTCTACCGACG
AGGTTTACGGAACCCTGGCGCCCGAGGCCCCTGCCTTCGCCGAAACCCATCCCTACGAGCCCAACAGCCCGTACTCCGCC
AGCAAGGCCGCCAGCGACCACCTCGTCCGGGCCTGGCACCACACCTACGGCCTGCCGGTGCTGACCACCAACTGCAGCAA
CAACTACGGCCCCTTCCACTTTCCCGAGAAGCTGATCCCCCTGATGATCGTCAACGCGCTGGCCGGCAAGCCGCTGCCCG
TGTATGGCGACGGCATGCAGATCCGCGACTGGCTCTATGTGCGCGACCACTGCAGCGCCATCCGCCGCGTGCTGCAGGCC
GGCCAGCCCGGCGAGACCTACAACGTCGGTGGCTGGAACGAAAAGCCCAACATCGAAATCGTGAAGACCATCTGCACTCT
GCTCGACGAACTGCGTCCGCGTGCCGATGGCGAGCGCTATGAAAGCCAGATCGCCTACGTGAAGGACCGCCCGGGCCACG
ACCGCCGCTATGCCATCGATGCCCGCAAGATCGAGCGCGAACTCGGATGGAAGCCCGCCGAGACCTTCGAGACCGGCATC
CGCAAGACCGTGGAGTGGTACCTCGCGCATGGCGAATGGGTGGATGCCGTGACCAGCGGCGCCTACCGCGAGTGGATCGA
CCGCCAGTACCGCAAGCCCGCCGTGCAGGACGCCGCCACCGCATGA

Upstream 100 bases:

>100_bases
TCCACCCTGCAGCCGTCATCGTGCGCTTCGTTACGGTCGTTTGTAACGCGATGGGATAATCCCGCGTTCGGCCGCAAGGC
GTCTTTGCTCAAAACGATCC

Downstream 100 bases:

>100_bases
ACGTCCTTCTCTTCGGCAAGGGCGGGCAGGTCGGCTGGGAACTGCAGCGCAGCCTGTCCGTCCTGGGCACGGTCACGGCC
CTCGATTTCGACAGCACTTC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MILVTGGAGFIGANFVLDWLACGDEPVVNLDKLTYAGNLQNLASLQGDARHLFVQGDIGDRELLTRLLAGHRPRAVVNFA
AESHVDRSIHGPEDFIQTNVVGTFRLLEAVREYWQSLPGDERAAFRFLHVSTDEVYGTLAPEAPAFAETHPYEPNSPYSA
SKAASDHLVRAWHHTYGLPVLTTNCSNNYGPFHFPEKLIPLMIVNALAGKPLPVYGDGMQIRDWLYVRDHCSAIRRVLQA
GQPGETYNVGGWNEKPNIEIVKTICTLLDELRPRADGERYESQIAYVKDRPGHDRRYAIDARKIERELGWKPAETFETGI
RKTVEWYLAHGEWVDAVTSGAYREWIDRQYRKPAVQDAATA

Sequences:

>Translated_361_residues
MILVTGGAGFIGANFVLDWLACGDEPVVNLDKLTYAGNLQNLASLQGDARHLFVQGDIGDRELLTRLLAGHRPRAVVNFA
AESHVDRSIHGPEDFIQTNVVGTFRLLEAVREYWQSLPGDERAAFRFLHVSTDEVYGTLAPEAPAFAETHPYEPNSPYSA
SKAASDHLVRAWHHTYGLPVLTTNCSNNYGPFHFPEKLIPLMIVNALAGKPLPVYGDGMQIRDWLYVRDHCSAIRRVLQA
GQPGETYNVGGWNEKPNIEIVKTICTLLDELRPRADGERYESQIAYVKDRPGHDRRYAIDARKIERELGWKPAETFETGI
RKTVEWYLAHGEWVDAVTSGAYREWIDRQYRKPAVQDAATA
>Mature_361_residues
MILVTGGAGFIGANFVLDWLACGDEPVVNLDKLTYAGNLQNLASLQGDARHLFVQGDIGDRELLTRLLAGHRPRAVVNFA
AESHVDRSIHGPEDFIQTNVVGTFRLLEAVREYWQSLPGDERAAFRFLHVSTDEVYGTLAPEAPAFAETHPYEPNSPYSA
SKAASDHLVRAWHHTYGLPVLTTNCSNNYGPFHFPEKLIPLMIVNALAGKPLPVYGDGMQIRDWLYVRDHCSAIRRVLQA
GQPGETYNVGGWNEKPNIEIVKTICTLLDELRPRADGERYESQIAYVKDRPGHDRRYAIDARKIERELGWKPAETFETGI
RKTVEWYLAHGEWVDAVTSGAYREWIDRQYRKPAVQDAATA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=330, Percent_Identity=37.2727272727273, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=23.1454005934718, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI56237023, Length=344, Percent_Identity=27.6162790697674, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI56118217, Length=344, Percent_Identity=27.6162790697674, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI189083684, Length=344, Percent_Identity=27.6162790697674, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI4504031, Length=353, Percent_Identity=25.7790368271955, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1788353, Length=357, Percent_Identity=61.0644257703081, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=60.4651162790698, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1786974, Length=355, Percent_Identity=27.3239436619718, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788366, Length=293, Percent_Identity=29.0102389078498, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=330, Percent_Identity=36.0606060606061, Blast_Score=204, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI115532424, Length=331, Percent_Identity=33.8368580060423, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17539532, Length=337, Percent_Identity=24.9258160237389, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17539422, Length=298, Percent_Identity=25.1677852348993, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI133901790, Length=298, Percent_Identity=25.1677852348993, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17539424, Length=298, Percent_Identity=25.1677852348993, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=298, Percent_Identity=25.503355704698, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI133901788, Length=298, Percent_Identity=25.1677852348993, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17507723, Length=304, Percent_Identity=25, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=360, Percent_Identity=25.5555555555556, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=25.3776435045317, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19923002, Length=357, Percent_Identity=27.4509803921569, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24158427, Length=340, Percent_Identity=26.7647058823529, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40553; Mature: 40553

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGANFVLDWLACGDEPVVNLDKLTYAGNLQNLASLQGDARHLFVQGDIGD
CEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHEEECCCHHHHHHCCCCCEEEEEECCCCC
RELLTRLLAGHRPRAVVNFAAESHVDRSIHGPEDFIQTNVVGTFRLLEAVREYWQSLPGD
HHHHHHHHCCCCCCCEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ERAAFRFLHVSTDEVYGTLAPEAPAFAETHPYEPNSPYSASKAASDHLVRAWHHTYGLPV
CHHEEEEEEECHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCE
LTTNCSNNYGPFHFPEKLIPLMIVNALAGKPLPVYGDGMQIRDWLYVRDHCSAIRRVLQA
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GQPGETYNVGGWNEKPNIEIVKTICTLLDELRPRADGERYESQIAYVKDRPGHDRRYAID
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHEEEEECCCCCCCEEEHH
ARKIERELGWKPAETFETGIRKTVEWYLAHGEWVDAVTSGAYREWIDRQYRKPAVQDAAT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCC
A
C
>Mature Secondary Structure
MILVTGGAGFIGANFVLDWLACGDEPVVNLDKLTYAGNLQNLASLQGDARHLFVQGDIGD
CEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHEEECCCHHHHHHCCCCCEEEEEECCCCC
RELLTRLLAGHRPRAVVNFAAESHVDRSIHGPEDFIQTNVVGTFRLLEAVREYWQSLPGD
HHHHHHHHCCCCCCCEEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
ERAAFRFLHVSTDEVYGTLAPEAPAFAETHPYEPNSPYSASKAASDHLVRAWHHTYGLPV
CHHEEEEEEECHHHHHEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCE
LTTNCSNNYGPFHFPEKLIPLMIVNALAGKPLPVYGDGMQIRDWLYVRDHCSAIRRVLQA
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GQPGETYNVGGWNEKPNIEIVKTICTLLDELRPRADGERYESQIAYVKDRPGHDRRYAID
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHEEEEECCCCCCCEEEHH
ARKIERELGWKPAETFETGIRKTVEWYLAHGEWVDAVTSGAYREWIDRQYRKPAVQDAAT
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]