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The map label for this gene is yiaO [H]

Identifier: 120612715

GI number: 120612715

Start: 4529785

End: 4530813

Strand: Direct

Name: yiaO [H]

Synonym: Aave_4078

Alternate gene names: 120612715

Gene position: 4529785-4530813 (Clockwise)

Preceding gene: 120612714

Following gene: 120612717

Centisome position: 84.63

GC content: 65.79

Gene sequence:

>1029_bases
ATGCAACTGCGCACCTTCCTTGCCACCGCCGTGGCCGCCGCGGCCGCACTCGCGTTCAGTGCCGCACCGGCCGCCGCGCA
GAACTACAAGAGCGAATACCGCATGTCGCTGGTGGTGGGCACCGCCTTCCCCTGGGGCAAGGGTGGCGAACTCTGGGCGA
ACAAGGTGCGCGAGCGCACGCAGGGTCGCATCAACATCAAGCTCTATCCGGGCGTCTCGCTCATCCAGGGTGACCAGACC
CGCGAGTTCAGCGCGCTGCGACAGGGCGTGATCGACATGGCCGTGGGCTCCACCATCAACTGGTCGCCGCAGGTCAAGGC
GCTCAACCTCTTCTCCCTGCCCTTCCTCTTCCCCGACTACGCCTCGGTGGACGTGGTCACGCAGGGCGAAGTCGGCAAGA
GCATCTTCCAGACCCTCGAAAAAGCCGGCGTGGTGCCGCTCGCCTGGGGCGAGAACGGCTACCGTGAGATCTCCAACTCC
AAGCGGCCCATCAAGACTCCCGCCGACCTCAAGGGGTTGAAGGTCCGCGTGGTCGGCTCGCCGCTGTTCCTCGACACCTT
CACGGCGCTGGGAGCCAACCCCACCCAGATGAGCTGGGCCGACGCGCAACCCGCCATGGCCAGCGGCGCCGTGGACGGCC
AGGAAAACCCCTTGTCCATCTACCTGGCCGCCAAGCTCTACACCGTGGCCCAGAAACATGTGACCCTGTGGGGTTACATG
AACGATCCGCTCATCTTCGTGGTGAACAAGGACATCTGGAACTCGTGGACGCCCGCGGACCGCGAGATCGTTCGCCAGGC
TGCCATCGAGGCCGGCAAGGAAGAAATCGCCATCGCGCGCAAGGGCCTGATCGAGACGGACAAGCCCCTGCTCAAGGAGA
TCGCCAGCCACGGCGTCACCGTGACGCAGCTCACCCCCGACGAGCGGCAGGCCTTCGTGCAGGCCACCCGCCCGGTCTTC
GACAAGTGGAAGGGACAGATCGGCGCGGACCTCGTGAACGCGGCGGAAAAGGCGGTGGCGAAGAGGTAG

Upstream 100 bases:

>100_bases
TCGCCTTTCCGCAACTGGCCCTCTGGCTGCCGGCGCGCCTGGGCTATTGAAGCCGCGCGCTTTTTCCCTTCCGAAACCGA
CGATCCACAGGAGACGACTC

Downstream 100 bases:

>100_bases
CGCACTCCCGGGCGCCACGGCGCTTCCCGGCAGGGGTCACTCTTCTCGCGACTGCTGGCGCCACATGTCCGCATAGCGCC
CCCCGAGCGCCAGCAACCGC

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MQLRTFLATAVAAAAALAFSAAPAAAQNYKSEYRMSLVVGTAFPWGKGGELWANKVRERTQGRINIKLYPGVSLIQGDQT
REFSALRQGVIDMAVGSTINWSPQVKALNLFSLPFLFPDYASVDVVTQGEVGKSIFQTLEKAGVVPLAWGENGYREISNS
KRPIKTPADLKGLKVRVVGSPLFLDTFTALGANPTQMSWADAQPAMASGAVDGQENPLSIYLAAKLYTVAQKHVTLWGYM
NDPLIFVVNKDIWNSWTPADREIVRQAAIEAGKEEIAIARKGLIETDKPLLKEIASHGVTVTQLTPDERQAFVQATRPVF
DKWKGQIGADLVNAAEKAVAKR

Sequences:

>Translated_342_residues
MQLRTFLATAVAAAAALAFSAAPAAAQNYKSEYRMSLVVGTAFPWGKGGELWANKVRERTQGRINIKLYPGVSLIQGDQT
REFSALRQGVIDMAVGSTINWSPQVKALNLFSLPFLFPDYASVDVVTQGEVGKSIFQTLEKAGVVPLAWGENGYREISNS
KRPIKTPADLKGLKVRVVGSPLFLDTFTALGANPTQMSWADAQPAMASGAVDGQENPLSIYLAAKLYTVAQKHVTLWGYM
NDPLIFVVNKDIWNSWTPADREIVRQAAIEAGKEEIAIARKGLIETDKPLLKEIASHGVTVTQLTPDERQAFVQATRPVF
DKWKGQIGADLVNAAEKAVAKR
>Mature_342_residues
MQLRTFLATAVAAAAALAFSAAPAAAQNYKSEYRMSLVVGTAFPWGKGGELWANKVRERTQGRINIKLYPGVSLIQGDQT
REFSALRQGVIDMAVGSTINWSPQVKALNLFSLPFLFPDYASVDVVTQGEVGKSIFQTLEKAGVVPLAWGENGYREISNS
KRPIKTPADLKGLKVRVVGSPLFLDTFTALGANPTQMSWADAQPAMASGAVDGQENPLSIYLAAKLYTVAQKHVTLWGYM
NDPLIFVVNKDIWNSWTPADREIVRQAAIEAGKEEIAIARKGLIETDKPLLKEIASHGVTVTQLTPDERQAFVQATRPVF
DKWKGQIGADLVNAAEKAVAKR

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=341, Percent_Identity=33.4310850439883, Blast_Score=171, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 37231; Mature: 37231

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLRTFLATAVAAAAALAFSAAPAAAQNYKSEYRMSLVVGTAFPWGKGGELWANKVRERT
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHH
QGRINIKLYPGVSLIQGDQTREFSALRQGVIDMAVGSTINWSPQVKALNLFSLPFLFPDY
CCEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCC
ASVDVVTQGEVGKSIFQTLEKAGVVPLAWGENGYREISNSKRPIKTPADLKGLKVRVVGS
CCEEEEECCHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEECC
PLFLDTFTALGANPTQMSWADAQPAMASGAVDGQENPLSIYLAAKLYTVAQKHVTLWGYM
CHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEEEHHHHHHHHHHHEEEEEEC
NDPLIFVVNKDIWNSWTPADREIVRQAAIEAGKEEIAIARKGLIETDKPLLKEIASHGVT
CCCEEEEEECHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCE
VTQLTPDERQAFVQATRPVFDKWKGQIGADLVNAAEKAVAKR
EEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQLRTFLATAVAAAAALAFSAAPAAAQNYKSEYRMSLVVGTAFPWGKGGELWANKVRERT
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHEEEEEEECCCCCCCCCHHHHHHHHHHH
QGRINIKLYPGVSLIQGDQTREFSALRQGVIDMAVGSTINWSPQVKALNLFSLPFLFPDY
CCEEEEEEECCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCC
ASVDVVTQGEVGKSIFQTLEKAGVVPLAWGENGYREISNSKRPIKTPADLKGLKVRVVGS
CCEEEEECCHHHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEECC
PLFLDTFTALGANPTQMSWADAQPAMASGAVDGQENPLSIYLAAKLYTVAQKHVTLWGYM
CHHHHHHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCEEEEEHHHHHHHHHHHEEEEEEC
NDPLIFVVNKDIWNSWTPADREIVRQAAIEAGKEEIAIARKGLIETDKPLLKEIASHGVT
CCCEEEEEECHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHCCCE
VTQLTPDERQAFVQATRPVFDKWKGQIGADLVNAAEKAVAKR
EEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]