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The map label for this gene is yeiE [H]

Identifier: 120612660

GI number: 120612660

Start: 4471374

End: 4472306

Strand: Reverse

Name: yeiE [H]

Synonym: Aave_4023

Alternate gene names: 120612660

Gene position: 4472306-4471374 (Counterclockwise)

Preceding gene: 120612663

Following gene: 120612658

Centisome position: 83.55

GC content: 71.92

Gene sequence:

>933_bases
ATGAACCAAAAAACAGGTCAATCGCCGGACCGGCTCCACCTGACGCTCCGGCACCTGGAGGTGTTTCTGGCGACCGCGCA
CGATGGGTCCACGCGGGCCGCCGCGGAGCGCATCGCCCGCTCGCAATCGGCCGCCAGCAGCGCGCTGGTGGAACTGGAGT
CGCTGCTGGGCGCAGCATTGTTCGATCGCGTGGGGCGTCGCCTGGTGCTCAACGAGAACGGCCGCGCCTTCCTGCCGCGG
GCCGCATCCCTGGTGGAGGCGGCCGGGGAACTGGAGCACCTCTTCCAGGGCCAGCATGCCGCTCCCCTGCGGGTGGCCGC
CAGCATGACCATCGGCGAACACATCCTTCCTGCCCTGCTGGCCCGCTGGAAGATGTCGCATCCCCAGAGCGCGGTGCAGT
TGCAGATCGCGAACACCACGGGCGTGCTGGCCGCCGTGGCGGCTTTCGACGCCGACATCGGTTTCATCGAGGGGCCGCAG
ACGCATGCCGACCTGGTGGTGCGCGAGTGGATGACCGACGAGATGGTGATCGTGGCGGCACCGGGCCATCCCCTCGCGCA
AAAGCCGTCGGCCACCCGGGCCGCCCTGCGCGCCGCCCGCTGGGCCCTGCGCGAGCGCGGCTCCGGCACGCGCGAGGCCG
CCGACCGCTGGCTGCTGGCGCAGCTCGGGCAGGTGCGGATCGACTTCGAGCTGGGCACGCCCGAAGCGGTCAAGGCCCTG
GTGGCCGCGGGGGACGCGCTGGCGCTGCTGCCCCGCCACAGCGTGGACTCCGCCCTGGCCCGCGGCGACCTGGTGGAACT
GCCGACCACGCTGCCTCGCGCGACCCGCACGCTGGCGGTGGTGACGCACCGGGAGCGGACGCTGGGGGCGGGGGGCGAGG
CGTTTTTGCGGTATTGCTTCGGCGGAGGCGCCTCGAAAAACGGTGATGCGTAG

Upstream 100 bases:

>100_bases
CCGCCAGCGGAGAAAGCCGCCGGAAGGCGTTCCATATGTCTTCATGGAGAATGAGCATATGTTTTTCGCATAAAGCAAAA
AACAGATAATTCGGTTCACC

Downstream 100 bases:

>100_bases
CTCTCCGTCCCCCGGGCTCTCCCTTACTTTCCTGCCGGTCCCTGGAACGTCTTCGTGCTGTACAGGTCCCGCGGCAACCG
CTTGAGCGTCGGCAACCCCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNQKTGQSPDRLHLTLRHLEVFLATAHDGSTRAAAERIARSQSAASSALVELESLLGAALFDRVGRRLVLNENGRAFLPR
AASLVEAAGELEHLFQGQHAAPLRVAASMTIGEHILPALLARWKMSHPQSAVQLQIANTTGVLAAVAAFDADIGFIEGPQ
THADLVVREWMTDEMVIVAAPGHPLAQKPSATRAALRAARWALRERGSGTREAADRWLLAQLGQVRIDFELGTPEAVKAL
VAAGDALALLPRHSVDSALARGDLVELPTTLPRATRTLAVVTHRERTLGAGGEAFLRYCFGGGASKNGDA

Sequences:

>Translated_310_residues
MNQKTGQSPDRLHLTLRHLEVFLATAHDGSTRAAAERIARSQSAASSALVELESLLGAALFDRVGRRLVLNENGRAFLPR
AASLVEAAGELEHLFQGQHAAPLRVAASMTIGEHILPALLARWKMSHPQSAVQLQIANTTGVLAAVAAFDADIGFIEGPQ
THADLVVREWMTDEMVIVAAPGHPLAQKPSATRAALRAARWALRERGSGTREAADRWLLAQLGQVRIDFELGTPEAVKAL
VAAGDALALLPRHSVDSALARGDLVELPTTLPRATRTLAVVTHRERTLGAGGEAFLRYCFGGGASKNGDA
>Mature_310_residues
MNQKTGQSPDRLHLTLRHLEVFLATAHDGSTRAAAERIARSQSAASSALVELESLLGAALFDRVGRRLVLNENGRAFLPR
AASLVEAAGELEHLFQGQHAAPLRVAASMTIGEHILPALLARWKMSHPQSAVQLQIANTTGVLAAVAAFDADIGFIEGPQ
THADLVVREWMTDEMVIVAAPGHPLAQKPSATRAALRAARWALRERGSGTREAADRWLLAQLGQVRIDFELGTPEAVKAL
VAAGDALALLPRHSVDSALARGDLVELPTTLPRATRTLAVVTHRERTLGAGGEAFLRYCFGGGASKNGDA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788481, Length=288, Percent_Identity=46.1805555555556, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI1788748, Length=291, Percent_Identity=27.8350515463918, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI87082132, Length=257, Percent_Identity=25.2918287937743, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=24.496644295302, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1787601, Length=279, Percent_Identity=27.5985663082437, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87081904, Length=290, Percent_Identity=26.551724137931, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790399, Length=271, Percent_Identity=23.2472324723247, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI157672245, Length=296, Percent_Identity=25.6756756756757, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1789204, Length=253, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=293, Percent_Identity=23.2081911262799, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787806, Length=246, Percent_Identity=25.609756097561, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788887, Length=191, Percent_Identity=26.1780104712042, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33000; Mature: 33000

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKTGQSPDRLHLTLRHLEVFLATAHDGSTRAAAERIARSQSAASSALVELESLLGAAL
CCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDRVGRRLVLNENGRAFLPRAASLVEAAGELEHLFQGQHAAPLRVAASMTIGEHILPALL
HHHCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
ARWKMSHPQSAVQLQIANTTGVLAAVAAFDADIGFIEGPQTHADLVVREWMTDEMVIVAA
HHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEC
PGHPLAQKPSATRAALRAARWALRERGSGTREAADRWLLAQLGQVRIDFELGTPEAVKAL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
VAAGDALALLPRHSVDSALARGDLVELPTTLPRATRTLAVVTHRERTLGAGGEAFLRYCF
HHCCCHHEECCCCCHHHHHHCCCCEECCCCCCHHHHEEEEEEECCHHCCCCHHHHHHHHH
GGGASKNGDA
CCCCCCCCCC
>Mature Secondary Structure
MNQKTGQSPDRLHLTLRHLEVFLATAHDGSTRAAAERIARSQSAASSALVELESLLGAAL
CCCCCCCCCHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDRVGRRLVLNENGRAFLPRAASLVEAAGELEHLFQGQHAAPLRVAASMTIGEHILPALL
HHHCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
ARWKMSHPQSAVQLQIANTTGVLAAVAAFDADIGFIEGPQTHADLVVREWMTDEMVIVAA
HHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEEEC
PGHPLAQKPSATRAALRAARWALRERGSGTREAADRWLLAQLGQVRIDFELGTPEAVKAL
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHH
VAAGDALALLPRHSVDSALARGDLVELPTTLPRATRTLAVVTHRERTLGAGGEAFLRYCF
HHCCCHHEECCCCCHHHHHHCCCCEECCCCCCHHHHEEEEEEECCHHCCCCHHHHHHHHH
GGGASKNGDA
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]