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The map label for this gene is pcaJ [H]

Identifier: 120612584

GI number: 120612584

Start: 4371003

End: 4371650

Strand: Reverse

Name: pcaJ [H]

Synonym: Aave_3945

Alternate gene names: 120612584

Gene position: 4371650-4371003 (Counterclockwise)

Preceding gene: 120612585

Following gene: 120612583

Centisome position: 81.67

GC content: 68.83

Gene sequence:

>648_bases
GTGAGCAGCTATCAGAAACGCAGCAAAGACGAACTGGCCCGGCGTGTGGCCCAGGACATCCACGAAGGCGCCGTCGTCAA
CCTGGGCATCGGGCAGCCCACCCTCGTGGCCAACCACATACCGGCCGACCGCGAAGTCATCCTGCAGAGCGAGAACGGCA
TCCTCGGCATGGGCCCCGCGCCCGCGGCCGGGCAGGAGGACTACGACCTCATCAACGCGGGCAAGCAGCCCGTCACGCTG
CTGCCGGGCGGCGCTTACTTCCACCATGCCGACAGCTTCGCCATGATGCGCGGCGGCCACCTGGACATCTGCGTGCTGGG
CGCCTTCCAGGTCAGCGCCACGGGCGACCTGGCCAACTGGAGCACGGGCGAGCCCGGGGCCATTCCGGCCGTGGGCGGCG
CCATGGACCTGGCCATCGGCGCCAAGCAGACCTGGGTGATGATGGACCTGCTGACCAAGCAGGGCCAGTCCAAGATCGTG
GCGCAGTGCACTTATCCGCTGACGGGCATCGGCTGCGTCAAGCGCATCTACACCGACCTCGCCACCCTGGCCTGCACGCC
GGACGGCCTGGTGCTGATCGACGCCGTGCAGGGCCTGGAGCACGGGGAACTCGAACGCCTCGTCGGCCTGTCCATCCGCG
CCGCCTGA

Upstream 100 bases:

>100_bases
CATCGAGCGCACCGCCACCCAGGGCGCGGGCTTCAGGAAGCCTGCCTGAGCCCGGCATCGCATCGGAATTGAACAGGCCA
CCAGCGCAGAAGGAATCAGC

Downstream 100 bases:

>100_bases
AACGCCGCGCCCTCAACCCCAAGGAGACAAGACACCATGACCTCTTCGCAAGCCTTCATCTGCGACGCCATCCGCACGCC
CTTCGGCCGCTACGGCGGCG

Product: butyryl-CoA:acetate CoA transferase

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSSYQKRSKDELARRVAQDIHEGAVVNLGIGQPTLVANHIPADREVILQSENGILGMGPAPAAGQEDYDLINAGKQPVTL
LPGGAYFHHADSFAMMRGGHLDICVLGAFQVSATGDLANWSTGEPGAIPAVGGAMDLAIGAKQTWVMMDLLTKQGQSKIV
AQCTYPLTGIGCVKRIYTDLATLACTPDGLVLIDAVQGLEHGELERLVGLSIRAA

Sequences:

>Translated_215_residues
MSSYQKRSKDELARRVAQDIHEGAVVNLGIGQPTLVANHIPADREVILQSENGILGMGPAPAAGQEDYDLINAGKQPVTL
LPGGAYFHHADSFAMMRGGHLDICVLGAFQVSATGDLANWSTGEPGAIPAVGGAMDLAIGAKQTWVMMDLLTKQGQSKIV
AQCTYPLTGIGCVKRIYTDLATLACTPDGLVLIDAVQGLEHGELERLVGLSIRAA
>Mature_214_residues
SSYQKRSKDELARRVAQDIHEGAVVNLGIGQPTLVANHIPADREVILQSENGILGMGPAPAAGQEDYDLINAGKQPVTLL
PGGAYFHHADSFAMMRGGHLDICVLGAFQVSATGDLANWSTGEPGAIPAVGGAMDLAIGAKQTWVMMDLLTKQGQSKIVA
QCTYPLTGIGCVKRIYTDLATLACTPDGLVLIDAVQGLEHGELERLVGLSIRAA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=204, Percent_Identity=45.5882352941176, Blast_Score=168, Evalue=3e-42,
Organism=Homo sapiens, GI11545841, Length=181, Percent_Identity=49.171270718232, Blast_Score=163, Evalue=1e-40,
Organism=Escherichia coli, GI1788552, Length=177, Percent_Identity=48.5875706214689, Blast_Score=166, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17531517, Length=211, Percent_Identity=47.39336492891, Blast_Score=174, Evalue=3e-44,
Organism=Drosophila melanogaster, GI21358325, Length=193, Percent_Identity=48.7046632124352, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24655794, Length=193, Percent_Identity=48.7046632124352, Blast_Score=177, Evalue=6e-45,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 22587; Mature: 22456

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYQKRSKDELARRVAQDIHEGAVVNLGIGQPTLVANHIPADREVILQSENGILGMGPA
CCCHHHCCHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCEEEEECCCCEEECCCC
PAAGQEDYDLINAGKQPVTLLPGGAYFHHADSFAMMRGGHLDICVLGAFQVSATGDLANW
CCCCCCCCHHHHCCCCCEEEECCCCEEECCCCCHHHCCCCEEEEEEEEEEEECCCCCCCC
STGEPGAIPAVGGAMDLAIGAKQTWVMMDLLTKQGQSKIVAQCTYPLTGIGCVKRIYTDL
CCCCCCCCCCCCCHHHEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHH
ATLACTPDGLVLIDAVQGLEHGELERLVGLSIRAA
HHHEECCCCEEEEEECCCCCCCCHHHHHCEEEECC
>Mature Secondary Structure 
SSYQKRSKDELARRVAQDIHEGAVVNLGIGQPTLVANHIPADREVILQSENGILGMGPA
CCHHHCCHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCCEEEEECCCCEEECCCC
PAAGQEDYDLINAGKQPVTLLPGGAYFHHADSFAMMRGGHLDICVLGAFQVSATGDLANW
CCCCCCCCHHHHCCCCCEEEECCCCEEECCCCCHHHCCCCEEEEEEEEEEEECCCCCCCC
STGEPGAIPAVGGAMDLAIGAKQTWVMMDLLTKQGQSKIVAQCTYPLTGIGCVKRIYTDL
CCCCCCCCCCCCCHHHEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHH
ATLACTPDGLVLIDAVQGLEHGELERLVGLSIRAA
HHHEECCCCEEEEEECCCCCCCCHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]