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The map label for this gene is azoB [H]

Identifier: 120612574

GI number: 120612574

Start: 4361141

End: 4362007

Strand: Direct

Name: azoB [H]

Synonym: Aave_3935

Alternate gene names: 120612574

Gene position: 4361141-4362007 (Clockwise)

Preceding gene: 120612573

Following gene: 120612575

Centisome position: 81.47

GC content: 67.13

Gene sequence:

>867_bases
ATGATCCTGGTTTGTGGAGCCACCGGCGGCATCGGCGGTGAATTGTGCAAGCTGCTGAAGGCGGCAGGAGCGCCTTTTCG
CGCCATGGCGCGCAGGCAGGAACAGGTGGACAGGTTCAGGCAACAGGGCATGGATGCCGTGCTCGGCGACTTCGACAGGC
CCGAGACGCTCGCAGCGGCCATGCAGGGCTGCGACACGATGTTCCTCATCACGCCGCCCAACCCCGACCAGTTCACCCAG
GAAACGGGCGCCATCGACGCCGCGAAGCGCGCGGGCGTGGGCCGGGTCGTGAAGATTTCCGCCTCCGATGGCAATGTGCG
CACGCCGGTTCCGTGGGCACGCACCCACGCGCTCATCGACCACCACCTGCGCGCCGCGGGTATCGGCTGGACCCTCCTGA
AGCCCACCGCCTTCATGCAGAACTTCCTGTGGTTCAAGAAGCCGATCGCCAAGGGCTTTCTGCCCCAGGTGACGGGCGAC
GGGTCGGTGTCATGGGTCGATACCCGTGACGTGGCCCGCGTCGCCGCCGCCGTTCTGACCCAGGAGGGCCACGCAGGGGC
CACCTACTTCCTGACCGGGCCGGAAACGCTGGACACGCACGAGGCGGCGAAGCGGCTGTCGCACGTGACCGGACACCAGG
TGCGCTACCTGAACCTGCCGACGTTCGTGTTCCGTGCCATGCTGCGGCTGACCGGCAACTCCGCCTGGATGGCCAGGGGC
CTGGTCGTGCAGTTCGCGGACGTCGTGGCCGGCCACCACGACATCGATCCGACCTTCGAAATCGAGCGCCTCACCGGCCA
GCCTGCGCGCCGGTTCGACGATTTCATTCGCGATCACCGCGATGCGTTTGCCCGGCATGGCTCGTAA

Upstream 100 bases:

>100_bases
TGGCGCCATCGCCACCCTCCACGCGATGGTGGAGGTGATCTCGGAGCAGCTCGGCTAGCCGGTGCCACACCCGTTTTCCC
ACTTGGAAGGAACCCTTGAC

Downstream 100 bases:

>100_bases
CCTTTCGAGCGGCAGCAGCATGATTTTCCCGACCAAGGTAGGCCTCGATGGTTGAAGCGCTCCGGACCATCGGGGACCAT
GGCATCGTCGGCGATCTCGA

Product: NmrA family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MILVCGATGGIGGELCKLLKAAGAPFRAMARRQEQVDRFRQQGMDAVLGDFDRPETLAAAMQGCDTMFLITPPNPDQFTQ
ETGAIDAAKRAGVGRVVKISASDGNVRTPVPWARTHALIDHHLRAAGIGWTLLKPTAFMQNFLWFKKPIAKGFLPQVTGD
GSVSWVDTRDVARVAAAVLTQEGHAGATYFLTGPETLDTHEAAKRLSHVTGHQVRYLNLPTFVFRAMLRLTGNSAWMARG
LVVQFADVVAGHHDIDPTFEIERLTGQPARRFDDFIRDHRDAFARHGS

Sequences:

>Translated_288_residues
MILVCGATGGIGGELCKLLKAAGAPFRAMARRQEQVDRFRQQGMDAVLGDFDRPETLAAAMQGCDTMFLITPPNPDQFTQ
ETGAIDAAKRAGVGRVVKISASDGNVRTPVPWARTHALIDHHLRAAGIGWTLLKPTAFMQNFLWFKKPIAKGFLPQVTGD
GSVSWVDTRDVARVAAAVLTQEGHAGATYFLTGPETLDTHEAAKRLSHVTGHQVRYLNLPTFVFRAMLRLTGNSAWMARG
LVVQFADVVAGHHDIDPTFEIERLTGQPARRFDDFIRDHRDAFARHGS
>Mature_288_residues
MILVCGATGGIGGELCKLLKAAGAPFRAMARRQEQVDRFRQQGMDAVLGDFDRPETLAAAMQGCDTMFLITPPNPDQFTQ
ETGAIDAAKRAGVGRVVKISASDGNVRTPVPWARTHALIDHHLRAAGIGWTLLKPTAFMQNFLWFKKPIAKGFLPQVTGD
GSVSWVDTRDVARVAAAVLTQEGHAGATYFLTGPETLDTHEAAKRLSHVTGHQVRYLNLPTFVFRAMLRLTGNSAWMARG
LVVQFADVVAGHHDIDPTFEIERLTGQPARRFDDFIRDHRDAFARHGS

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Uses preferentially NADPH rather than NADH as an electron donor for its activity. The enzyme reductively cleaved Orange II and carboxy-Orange II, and can a

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NmrA-type oxidoreductase family. Azoreductase type 3 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790656, Length=283, Percent_Identity=27.5618374558304, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR008030 [H]

Pfam domain/function: PF05368 NmrA [H]

EC number: NA

Molecular weight: Translated: 31540; Mature: 31540

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVCGATGGIGGELCKLLKAAGAPFRAMARRQEQVDRFRQQGMDAVLGDFDRPETLAAA
CEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHH
MQGCDTMFLITPPNPDQFTQETGAIDAAKRAGVGRVVKISASDGNVRTPVPWARTHALID
HHCCCEEEEEECCCCHHHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
HHLRAAGIGWTLLKPTAFMQNFLWFKKPIAKGFLPQVTGDGSVSWVDTRDVARVAAAVLT
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHH
QEGHAGATYFLTGPETLDTHEAAKRLSHVTGHQVRYLNLPTFVFRAMLRLTGNSAWMARG
CCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCHHHHHH
LVVQFADVVAGHHDIDPTFEIERLTGQPARRFDDFIRDHRDAFARHGS
HHHHHHHHHCCCCCCCCCEEEHCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MILVCGATGGIGGELCKLLKAAGAPFRAMARRQEQVDRFRQQGMDAVLGDFDRPETLAAA
CEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHH
MQGCDTMFLITPPNPDQFTQETGAIDAAKRAGVGRVVKISASDGNVRTPVPWARTHALID
HHCCCEEEEEECCCCHHHHHHHCCHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHH
HHLRAAGIGWTLLKPTAFMQNFLWFKKPIAKGFLPQVTGDGSVSWVDTRDVARVAAAVLT
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHHHHHH
QEGHAGATYFLTGPETLDTHEAAKRLSHVTGHQVRYLNLPTFVFRAMLRLTGNSAWMARG
CCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCHHHHHH
LVVQFADVVAGHHDIDPTFEIERLTGQPARRFDDFIRDHRDAFARHGS
HHHHHHHHHCCCCCCCCCEEEHCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12147495 [H]