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The map label for this gene is cpdB [H]

Identifier: 120612424

GI number: 120612424

Start: 4200455

End: 4202572

Strand: Reverse

Name: cpdB [H]

Synonym: Aave_3782

Alternate gene names: 120612424

Gene position: 4202572-4200455 (Counterclockwise)

Preceding gene: 120612433

Following gene: 120612422

Centisome position: 78.51

GC content: 66.38

Gene sequence:

>2118_bases
ATGTCACACGGCCGCCCAACACTTGCGGCTTTCTTTCGTTACCTCTTCTGCCCTATGTCTCCGCATTCCTCCTTCGCGGC
CCCGCGCCTGCCCCGGTTCCCGCTGATCGCCCTGTGCGCGATTCCCTCGCTGTTCCTGGCCGCATGCGGCGGCGGCGGCG
ACGGCAATCCGCCCGCCAGCGCGGGAGCCACGGCCACGCTGGCCGTGCTGGAAACCACCGACCTGCACTTCAACGTGCGC
AGCTACGACTACTTCAAGCTCGCCGAAGACAAGTCGTATGGCTTCGAGCGCACGGCCACCCTGGTGCGCCAGGCGCGCAA
GGAATTCCCGAACACGCTGCTGGTGGACAACGGAGACACCATCCAGGGCACGGCGCTGGCCGACTACGAAGCCGTGGTCA
AGACGATTCCATGCAACCAGCAGCTCTCGATGTACAAGGCGATGGGCGCCCTGGGCTTCGACGCAGGCACGCTGGGCAAC
CACGAGTTCAACTACGGCCTGCCGTTCCTGAACCAGGTGCTGGGCGGCGGCATGCAGGTCGATGGCGTGGATTCCGGCGT
GAAGTGCGCCGGTTCGGGCTACCCCACCGTGCTCGCCAACGTGTACAGCAGCAAGACCCGCCAGCCGCTGATCCAGCCCT
ACACGCTGATCGAGAAGACCCTGGTCGCCACCGGCACCGACGGCAAGGAAGTGAAGCTGCCGATCAAGATCGGCGTGATC
GGCTTCACCACGCCCGGGATCATGAACTGGGACAAGCGCTATCTGGAAGGCAAGGTCTACACCGAAGGCGCCGTGGAGTC
CGCCACCAAGTACGTGCCGGAGCTGCGCGCCAAGGGCGCCGACGTGGTCGTGGCGCTGCTGCACGGCGGCCTGGACGCCG
GCCCGTACTCGGCCACGATGGAGAACCCGGGCCTGTACCTCTCGAAGGTGTCGGGCATCGATGCGATGGTGATGGGCCAC
CAGCACAGCGTGTTCCCCGACACGGCGGCCACGCCGGCCTACACCCAGGCGGGCGTGGACAACAAGGCCGGCACCATCAA
CGGCGTGCCGGCCGTGATGGCCAGTTCGTGGGGCAAGGCGCTGGGCGTGGTCCGCCTCGCGCTGAAGTGGGACGGCAAGG
CCTGGTCGGTGGACAAGGGCGCCTCGAAGAGCGAGCTGCGCAACATCCAGACGGGCAAGGACGCCGCGGGCAAGGCCGTG
TTCGTGGATGCCGACAGCAGCACCGCGCCGCTGGTCGAGGCGCAGCACCAGAACACCATCGGCTACGTGAAGACGCCGAT
CGGCACCACCGACTTCCGCATGAGCACGCTGTTCGCCGACGTGGGCGACCCGGGCGCGATCCAGATCGTGAACCAGGCGC
AGCAGGCCTATGTGTCGGCCTACATCCAGGCCAACCTGCCGCAGTACAAGAGCCTGCCGGTGCTATCGGTGAGCGCGCCC
TTCAAGTCCGGCTTCCAGGGCGGCGCCGACTACACCGACGTGGCCACGGGCCCGCTCGCGATCTTCAACGCGGCCGACCT
CTACCTGTATCCCAATACCGTGTATGCGGTGAAGGTGAACGGCGCGGACATCAAGAACTGGCTGGAGGCCGCCGCCAAGC
GCTTCAACCAGATCGACCCGGCGAAGACGTCCGAGCAGGCGCTGATCAGCACCTTCCCGGGCTACAACTTCGACATGTTC
ACCACGGCCGACGTGCAGTACGAGATCGACGTCACCCAGGCCGTCGGCAGCCGCATCAAGAACCTGACCTACAAGGGCCA
GGCGATCGACCCGGCGCAGGAGTTCGTGATCGCCACGAACAATTACCGCGCGACCAGCGGAAGGAGTTTCATCGACAAGC
TGGACGGCTCCGGCACGATCTGGGCGTCGCCCGACGCGAACCGCGACGTGGTGATCGACTACATCCGCAAGAACCCGTCC
GTGACGCGCACGGCCAACGGCAGCGCCAGGAGCTGGCGCTTCACCAAGGTGAAGACCGCCGGCCCGGTGGTGTTCGCCTC
CGGCGCCAATGCGCTGTCCGTGGCGCAGGCCGCGGGCCTGGCCAACATCTCCGTGCTGGCCGCGGACGATGGATCGGGCA
AGGGCACGTCGAAGTACGCGATCGATCTGTCGAAGTAA

Upstream 100 bases:

>100_bases
CGTTCCACCGTCGAGCCAATTGCATCCAAATGTTCGATGTAGAGACGACGAAGGCGGCAATTGCCGGCGGATCATGCCGC
CTGCACATGGAGTCATGGCG

Downstream 100 bases:

>100_bases
CCAGGGAGCGGCAGGCGTCCGGCAGGTGCCGCCGGCGGGCCTGCCGGGTCCGCCGGCGGCACCCGGGGACGTCAGTCGTC
CAGCTTTTCGCGGAGCACCT

Product: 5'-nucleotidase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 705; Mature: 704

Protein sequence:

>705_residues
MSHGRPTLAAFFRYLFCPMSPHSSFAAPRLPRFPLIALCAIPSLFLAACGGGGDGNPPASAGATATLAVLETTDLHFNVR
SYDYFKLAEDKSYGFERTATLVRQARKEFPNTLLVDNGDTIQGTALADYEAVVKTIPCNQQLSMYKAMGALGFDAGTLGN
HEFNYGLPFLNQVLGGGMQVDGVDSGVKCAGSGYPTVLANVYSSKTRQPLIQPYTLIEKTLVATGTDGKEVKLPIKIGVI
GFTTPGIMNWDKRYLEGKVYTEGAVESATKYVPELRAKGADVVVALLHGGLDAGPYSATMENPGLYLSKVSGIDAMVMGH
QHSVFPDTAATPAYTQAGVDNKAGTINGVPAVMASSWGKALGVVRLALKWDGKAWSVDKGASKSELRNIQTGKDAAGKAV
FVDADSSTAPLVEAQHQNTIGYVKTPIGTTDFRMSTLFADVGDPGAIQIVNQAQQAYVSAYIQANLPQYKSLPVLSVSAP
FKSGFQGGADYTDVATGPLAIFNAADLYLYPNTVYAVKVNGADIKNWLEAAAKRFNQIDPAKTSEQALISTFPGYNFDMF
TTADVQYEIDVTQAVGSRIKNLTYKGQAIDPAQEFVIATNNYRATSGRSFIDKLDGSGTIWASPDANRDVVIDYIRKNPS
VTRTANGSARSWRFTKVKTAGPVVFASGANALSVAQAAGLANISVLAADDGSGKGTSKYAIDLSK

Sequences:

>Translated_705_residues
MSHGRPTLAAFFRYLFCPMSPHSSFAAPRLPRFPLIALCAIPSLFLAACGGGGDGNPPASAGATATLAVLETTDLHFNVR
SYDYFKLAEDKSYGFERTATLVRQARKEFPNTLLVDNGDTIQGTALADYEAVVKTIPCNQQLSMYKAMGALGFDAGTLGN
HEFNYGLPFLNQVLGGGMQVDGVDSGVKCAGSGYPTVLANVYSSKTRQPLIQPYTLIEKTLVATGTDGKEVKLPIKIGVI
GFTTPGIMNWDKRYLEGKVYTEGAVESATKYVPELRAKGADVVVALLHGGLDAGPYSATMENPGLYLSKVSGIDAMVMGH
QHSVFPDTAATPAYTQAGVDNKAGTINGVPAVMASSWGKALGVVRLALKWDGKAWSVDKGASKSELRNIQTGKDAAGKAV
FVDADSSTAPLVEAQHQNTIGYVKTPIGTTDFRMSTLFADVGDPGAIQIVNQAQQAYVSAYIQANLPQYKSLPVLSVSAP
FKSGFQGGADYTDVATGPLAIFNAADLYLYPNTVYAVKVNGADIKNWLEAAAKRFNQIDPAKTSEQALISTFPGYNFDMF
TTADVQYEIDVTQAVGSRIKNLTYKGQAIDPAQEFVIATNNYRATSGRSFIDKLDGSGTIWASPDANRDVVIDYIRKNPS
VTRTANGSARSWRFTKVKTAGPVVFASGANALSVAQAAGLANISVLAADDGSGKGTSKYAIDLSK
>Mature_704_residues
SHGRPTLAAFFRYLFCPMSPHSSFAAPRLPRFPLIALCAIPSLFLAACGGGGDGNPPASAGATATLAVLETTDLHFNVRS
YDYFKLAEDKSYGFERTATLVRQARKEFPNTLLVDNGDTIQGTALADYEAVVKTIPCNQQLSMYKAMGALGFDAGTLGNH
EFNYGLPFLNQVLGGGMQVDGVDSGVKCAGSGYPTVLANVYSSKTRQPLIQPYTLIEKTLVATGTDGKEVKLPIKIGVIG
FTTPGIMNWDKRYLEGKVYTEGAVESATKYVPELRAKGADVVVALLHGGLDAGPYSATMENPGLYLSKVSGIDAMVMGHQ
HSVFPDTAATPAYTQAGVDNKAGTINGVPAVMASSWGKALGVVRLALKWDGKAWSVDKGASKSELRNIQTGKDAAGKAVF
VDADSSTAPLVEAQHQNTIGYVKTPIGTTDFRMSTLFADVGDPGAIQIVNQAQQAYVSAYIQANLPQYKSLPVLSVSAPF
KSGFQGGADYTDVATGPLAIFNAADLYLYPNTVYAVKVNGADIKNWLEAAAKRFNQIDPAKTSEQALISTFPGYNFDMFT
TADVQYEIDVTQAVGSRIKNLTYKGQAIDPAQEFVIATNNYRATSGRSFIDKLDGSGTIWASPDANRDVVIDYIRKNPSV
TRTANGSARSWRFTKVKTAGPVVFASGANALSVAQAAGLANISVLAADDGSGKGTSKYAIDLSK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=669, Percent_Identity=42.0029895366218, Blast_Score=433, Evalue=1e-122,
Organism=Escherichia coli, GI1786687, Length=564, Percent_Identity=21.4539007092199, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 74808; Mature: 74677

Theoretical pI: Translated: 8.14; Mature: 8.14

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHGRPTLAAFFRYLFCPMSPHSSFAAPRLPRFPLIALCAIPSLFLAACGGGGDGNPPAS
CCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
AGATATLAVLETTDLHFNVRSYDYFKLAEDKSYGFERTATLVRQARKEFPNTLLVDNGDT
CCCEEEEEEEEEECEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCE
IQGTALADYEAVVKTIPCNQQLSMYKAMGALGFDAGTLGNHEFNYGLPFLNQVLGGGMQV
EECCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCEEE
DGVDSGVKCAGSGYPTVLANVYSSKTRQPLIQPYTLIEKTLVATGTDGKEVKLPIKIGVI
CCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHEEECCCCCEEEEEEEEEEE
GFTTPGIMNWDKRYLEGKVYTEGAVESATKYVPELRAKGADVVVALLHGGLDAGPYSATM
EECCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCCCC
ENPGLYLSKVSGIDAMVMGHQHSVFPDTAATPAYTQAGVDNKAGTINGVPAVMASSWGKA
CCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHH
LGVVRLALKWDGKAWSVDKGASKSELRNIQTGKDAAGKAVFVDADSSTAPLVEAQHQNTI
EEEEEEEEEECCCEEECCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCEEECCCCCCC
GYVKTPIGTTDFRMSTLFADVGDPGAIQIVNQAQQAYVSAYIQANLPQYKSLPVLSVSAP
CEEECCCCCCCEEHHHHEECCCCCCEEEHHHHHHHHHHHHHEECCCCCCCCCCEEEECCC
FKSGFQGGADYTDVATGPLAIFNAADLYLYPNTVYAVKVNGADIKNWLEAAAKRFNQIDP
CCCCCCCCCCCCCCCCCCEEEEECCEEEEECCEEEEEEECCCCHHHHHHHHHHHHCCCCC
AKTSEQALISTFPGYNFDMFTTADVQYEIDVTQAVGSRIKNLTYKGQAIDPAQEFVIATN
CCCCCHHHHHHCCCCCEEEEEECCEEEEEEHHHHHHHHHHCCEECCCCCCCCCEEEEEEC
NYRATSGRSFIDKLDGSGTIWASPDANRDVVIDYIRKNPSVTRTANGSARSWRFTKVKTA
CEECCCCCHHHHHCCCCCEEEECCCCCCCEEEEEECCCCCEEEECCCCCCEEEEEEEECC
GPVVFASGANALSVAQAAGLANISVLAADDGSGKGTSKYAIDLSK
CCEEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEECC
>Mature Secondary Structure 
SHGRPTLAAFFRYLFCPMSPHSSFAAPRLPRFPLIALCAIPSLFLAACGGGGDGNPPAS
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
AGATATLAVLETTDLHFNVRSYDYFKLAEDKSYGFERTATLVRQARKEFPNTLLVDNGDT
CCCEEEEEEEEEECEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCE
IQGTALADYEAVVKTIPCNQQLSMYKAMGALGFDAGTLGNHEFNYGLPFLNQVLGGGMQV
EECCEEHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCEEE
DGVDSGVKCAGSGYPTVLANVYSSKTRQPLIQPYTLIEKTLVATGTDGKEVKLPIKIGVI
CCCCCCCEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHEEECCCCCEEEEEEEEEEE
GFTTPGIMNWDKRYLEGKVYTEGAVESATKYVPELRAKGADVVVALLHGGLDAGPYSATM
EECCCCCCCCCHHHHCCEEEECCHHHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCCCC
ENPGLYLSKVSGIDAMVMGHQHSVFPDTAATPAYTQAGVDNKAGTINGVPAVMASSWGKA
CCCCEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCHHHHHHHHHHH
LGVVRLALKWDGKAWSVDKGASKSELRNIQTGKDAAGKAVFVDADSSTAPLVEAQHQNTI
EEEEEEEEEECCCEEECCCCCCHHHHHHCCCCCCCCCCEEEEECCCCCCCEEECCCCCCC
GYVKTPIGTTDFRMSTLFADVGDPGAIQIVNQAQQAYVSAYIQANLPQYKSLPVLSVSAP
CEEECCCCCCCEEHHHHEECCCCCCEEEHHHHHHHHHHHHHEECCCCCCCCCCEEEECCC
FKSGFQGGADYTDVATGPLAIFNAADLYLYPNTVYAVKVNGADIKNWLEAAAKRFNQIDP
CCCCCCCCCCCCCCCCCCEEEEECCEEEEECCEEEEEEECCCCHHHHHHHHHHHHCCCCC
AKTSEQALISTFPGYNFDMFTTADVQYEIDVTQAVGSRIKNLTYKGQAIDPAQEFVIATN
CCCCCHHHHHHCCCCCEEEEEECCEEEEEEHHHHHHHHHHCCEECCCCCCCCCEEEEEEC
NYRATSGRSFIDKLDGSGTIWASPDANRDVVIDYIRKNPSVTRTANGSARSWRFTKVKTA
CEECCCCCHHHHHCCCCCEEEECCCCCCCEEEEEECCCCCEEEECCCCCCEEEEEEEECC
GPVVFASGANALSVAQAAGLANISVLAADDGSGKGTSKYAIDLSK
CCEEEECCCCHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3005231; 7610040; 9278503; 2172762; 9298646 [H]