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The map label for this gene is gudD [H]

Identifier: 120612269

GI number: 120612269

Start: 3995772

End: 3997127

Strand: Reverse

Name: gudD [H]

Synonym: Aave_3623

Alternate gene names: 120612269

Gene position: 3997127-3995772 (Counterclockwise)

Preceding gene: 120612270

Following gene: 120612268

Centisome position: 74.67

GC content: 71.17

Gene sequence:

>1356_bases
ATGACCTCTTCCGAGCACCTCCAGGCCCCGGGCGCCACGCCCGTCATCACCGACCTGCGCGTCGTGCCCGTGGCCGGCCA
CGACAGCATGCTGCTGAACCTGAGCGGCGCGCACGGCCCCTTCTTCACGCGCAACCTGCTGATCCTGCGCGACAGCGCCG
GCCGCACCGGCGTGGGCGAAGTGCCGGGCGGCGAGAAGATCCGGCAGACGCTCGAAGACGCGCGCGCGCTGATCGTGGGG
CAGGGCGTGGGCAACCACCGCGCGGTGCTGAACGCGATGCGCGCGCGCTTCGCCGACCGCGACAGCGGTGGCCGCGGCCT
GCAGACGTTCGATCTGCGCGTGACCATCCATGCCCTCACCGCCGTGGAATCGTGCTTTCTCGACCTGCTGGGCCAGCACC
TGGGCGTACCCGTGGCCGCATTGCTCGGCGACGGGCAGCAGCGCGAGGCCGTGCAGGTGCTGGGCTACCTGTTCTACGTG
GGCGACCGCCGCAAGACCGACCTGGCCTACCGCGACGAATCCGGGGCGCAGGACGACTGGTTCCGCCTGCGCAACGAGGA
AGCGCTCACGCCCGAGGCCATCGTGCGCCTGGCCGAGGCCACCCACGCGCGCTACGGCTTCGCCGACTTCAAGCTCAAGG
GCGGCGTGCTGCGCGGCGAGGACGAGGTGGAGGCGATCCGCGCGCTGCACGAGCGCTTCCCGCAGGCGCGCGTCACGCTC
GATCCCAACGGCGGCTGGCTGCTGAAGGACGCCGTGCGCCTCACCCGTGACCTGCACGGCGTGATGGCCTATGCCGAAGA
CCCCTGCGGCGCCGAGGGCGTGTATTCCGGCCGCGAGGTGATGGCCGAATTCCGCCGCGCCACCGGCCTGCCCACGGCCA
CCAACATGGTCGCCACCGACTGGCGCGAGATGTCGCACAGCCTGTCGCTGCAATCGGTGGACATCCCGCTCGCCGACCCG
CATTTCTGGACCATGGCCGGATCGGTGCGCGTGGCACAGGTGTGCGAGGCCTTCGGCCTGACCTGGGGCTCGCACTCCAA
CAACCACTTCGACGTGTCGCTCGCCATGTTCACCCACGTGGCGGCCGCGGCGCCCGGCAAGGTCACGGCCATCGACACCC
ACTGGATCTGGCAGGACGGCCAGCACCTCACATGCGAACCCTGCCGCATCGAGGGCGGGCAGATCCAGGTGCCGCAGCGC
CCCGGCCTGGGGCTGGAGCTGGACATGGACGCGGTGGAGGCCGCGCACCGGCTCTACCTGCAGCACGGCCTGGGCGCGCG
CGACGATGCGGTCGCCATGCAGTACCTCGTGCCGGGCTGGCGCTTCGACAACAAGCGGCCCTGCATGGTGCGCTGA

Upstream 100 bases:

>100_bases
GGCGTCCGCTGGCGTGGCCTGCCCAGCGGCCATCTGGTGGTTGCACGGCGCGCCGGATAGGGCACCGGGTGCAGTACTTA
CTTTCCTTTCCTCATTCACC

Downstream 100 bases:

>100_bases
GGGCGCGGCACCGCCTGCCGGTTCGGGCGGATGCCTTACAGCCTCGCCGGGCAGGGCGCCATCGTGTCCAAGTTCTGCAG
CGCCGGCCAGACCTGCGTGT

Product: glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTSSEHLQAPGATPVITDLRVVPVAGHDSMLLNLSGAHGPFFTRNLLILRDSAGRTGVGEVPGGEKIRQTLEDARALIVG
QGVGNHRAVLNAMRARFADRDSGGRGLQTFDLRVTIHALTAVESCFLDLLGQHLGVPVAALLGDGQQREAVQVLGYLFYV
GDRRKTDLAYRDESGAQDDWFRLRNEEALTPEAIVRLAEATHARYGFADFKLKGGVLRGEDEVEAIRALHERFPQARVTL
DPNGGWLLKDAVRLTRDLHGVMAYAEDPCGAEGVYSGREVMAEFRRATGLPTATNMVATDWREMSHSLSLQSVDIPLADP
HFWTMAGSVRVAQVCEAFGLTWGSHSNNHFDVSLAMFTHVAAAAPGKVTAIDTHWIWQDGQHLTCEPCRIEGGQIQVPQR
PGLGLELDMDAVEAAHRLYLQHGLGARDDAVAMQYLVPGWRFDNKRPCMVR

Sequences:

>Translated_451_residues
MTSSEHLQAPGATPVITDLRVVPVAGHDSMLLNLSGAHGPFFTRNLLILRDSAGRTGVGEVPGGEKIRQTLEDARALIVG
QGVGNHRAVLNAMRARFADRDSGGRGLQTFDLRVTIHALTAVESCFLDLLGQHLGVPVAALLGDGQQREAVQVLGYLFYV
GDRRKTDLAYRDESGAQDDWFRLRNEEALTPEAIVRLAEATHARYGFADFKLKGGVLRGEDEVEAIRALHERFPQARVTL
DPNGGWLLKDAVRLTRDLHGVMAYAEDPCGAEGVYSGREVMAEFRRATGLPTATNMVATDWREMSHSLSLQSVDIPLADP
HFWTMAGSVRVAQVCEAFGLTWGSHSNNHFDVSLAMFTHVAAAAPGKVTAIDTHWIWQDGQHLTCEPCRIEGGQIQVPQR
PGLGLELDMDAVEAAHRLYLQHGLGARDDAVAMQYLVPGWRFDNKRPCMVR
>Mature_450_residues
TSSEHLQAPGATPVITDLRVVPVAGHDSMLLNLSGAHGPFFTRNLLILRDSAGRTGVGEVPGGEKIRQTLEDARALIVGQ
GVGNHRAVLNAMRARFADRDSGGRGLQTFDLRVTIHALTAVESCFLDLLGQHLGVPVAALLGDGQQREAVQVLGYLFYVG
DRRKTDLAYRDESGAQDDWFRLRNEEALTPEAIVRLAEATHARYGFADFKLKGGVLRGEDEVEAIRALHERFPQARVTLD
PNGGWLLKDAVRLTRDLHGVMAYAEDPCGAEGVYSGREVMAEFRRATGLPTATNMVATDWREMSHSLSLQSVDIPLADPH
FWTMAGSVRVAQVCEAFGLTWGSHSNNHFDVSLAMFTHVAAAAPGKVTAIDTHWIWQDGQHLTCEPCRIEGGQIQVPQRP
GLGLELDMDAVEAAHRLYLQHGLGARDDAVAMQYLVPGWRFDNKRPCMVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=440, Percent_Identity=71.1363636363636, Blast_Score=683, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=443, Percent_Identity=60.2708803611738, Blast_Score=541, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49436; Mature: 49304

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSEHLQAPGATPVITDLRVVPVAGHDSMLLNLSGAHGPFFTRNLLILRDSAGRTGVGE
CCCCCCCCCCCCCCEEECEEEEEECCCCEEEEEECCCCCCCEECEEEEEECCCCCCCCCC
VPGGEKIRQTLEDARALIVGQGVGNHRAVLNAMRARFADRDSGGRGLQTFDLRVTIHALT
CCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEHHHH
AVESCFLDLLGQHLGVPVAALLGDGQQREAVQVLGYLFYVGDRRKTDLAYRDESGAQDDW
HHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCHH
FRLRNEEALTPEAIVRLAEATHARYGFADFKLKGGVLRGEDEVEAIRALHERFPQARVTL
HHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCEECCCHHHHHHHHHHHHCCCCEEEE
DPNGGWLLKDAVRLTRDLHGVMAYAEDPCGAEGVYSGREVMAEFRRATGLPTATNMVATD
CCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
WREMSHSLSLQSVDIPLADPHFWTMAGSVRVAQVCEAFGLTWGSHSNNHFDVSLAMFTHV
HHHHHHCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHH
AAAAPGKVTAIDTHWIWQDGQHLTCEPCRIEGGQIQVPQRPGLGLELDMDAVEAAHRLYL
HHCCCCCEEEEEEEEEEECCCEEECCCCEECCCEEECCCCCCCCEEECHHHHHHHHHHHH
QHGLGARDDAVAMQYLVPGWRFDNKRPCMVR
HHCCCCCCHHHHHHHHCCCEECCCCCCCCCC
>Mature Secondary Structure 
TSSEHLQAPGATPVITDLRVVPVAGHDSMLLNLSGAHGPFFTRNLLILRDSAGRTGVGE
CCCCCCCCCCCCCEEECEEEEEECCCCEEEEEECCCCCCCEECEEEEEECCCCCCCCCC
VPGGEKIRQTLEDARALIVGQGVGNHRAVLNAMRARFADRDSGGRGLQTFDLRVTIHALT
CCCHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEHHHH
AVESCFLDLLGQHLGVPVAALLGDGQQREAVQVLGYLFYVGDRRKTDLAYRDESGAQDDW
HHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCHH
FRLRNEEALTPEAIVRLAEATHARYGFADFKLKGGVLRGEDEVEAIRALHERFPQARVTL
HHCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCEECCCHHHHHHHHHHHHCCCCEEEE
DPNGGWLLKDAVRLTRDLHGVMAYAEDPCGAEGVYSGREVMAEFRRATGLPTATNMVATD
CCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
WREMSHSLSLQSVDIPLADPHFWTMAGSVRVAQVCEAFGLTWGSHSNNHFDVSLAMFTHV
HHHHHHCCCEEEECCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHH
AAAAPGKVTAIDTHWIWQDGQHLTCEPCRIEGGQIQVPQRPGLGLELDMDAVEAAHRLYL
HHCCCCCEEEEEEEEEEECCCEEECCCCEECCCEEECCCCCCCCEEECHHHHHHHHHHHH
QHGLGARDDAVAMQYLVPGWRFDNKRPCMVR
HHCCCCCCHHHHHHHHCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]