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The map label for this gene is spoT [H]

Identifier: 120612232

GI number: 120612232

Start: 3955327

End: 3957594

Strand: Direct

Name: spoT [H]

Synonym: Aave_3585

Alternate gene names: 120612232

Gene position: 3955327-3957594 (Clockwise)

Preceding gene: 120612231

Following gene: 120612236

Centisome position: 73.89

GC content: 67.68

Gene sequence:

>2268_bases
ATGAATGAGGTCCTGACCAAACCCTCCGCCCCGGAGCCGCGCCCCGGCAGCGCCCCGGCGGCGGGCGCGTCCGCGGCGGC
CGCCAATGCGGCCGCCGCCAGTTTCGCCTCGCTCACCGAGAACCTCGATTACCTCGATGCGGCCAGCATCGAGCAGGTGC
GGCAGGCCTACAAATTCGCTGACAAGGCCCACCTGGGGCAACTGCGCAGCAGCGGCGAGCCCTACATCACCCATCCCATC
GCGGTGGCCGCCCAGTGCGCCACCTGGAAGCTCGACGCCCAGGCCCTGATGGCAGCGCTGCTGCACGATGCGATGGAAGA
CTGCGGCGTCACGAAGGCCGACCTGCTGGAACGCTTCGGCCCGCAGGTCGCCGAACTCGTGGACGGCCTCACCAAGCTCG
ACAAACTGCAGTTCAACACCCGCGAGGAGAACCAGGCCGAGTCCTTCCGCAAGATGCTGCTGGCGATGGCGCGCGACGTG
CGTGTCATCCTCATCAAGCTGGCGGACCGCACGCACAACATGCGCACGCTCTCGGACATGCCGCGCACCAAGTGGGGCCG
CATCGCATCCGAGACGCTCGAGATCTACGCCCCCATCGCCCACCGCCTGGGCCTGAACCAGACCTACCGCGAGCTCCAGG
ACCTCTCCTTCCGGCACCTGCATCCCTGGCGCTACGCCACCCTCTCCAAGGCCGTGAACAAGGCGCGCAACCGCCGGCGC
GACCTCATCCAGAAGGTGCAGGCCGAGGTGGACGCCGCCTTCGCCCGCATCGGCATGAAGGCCCGGCTCGCCGGCCGCGA
AAAGACGCTCTACGCCATCTACCAGAAGATGGACACCAAGCACCTGAGCTTCGCCCAGGTGACGGACATCTACGGCTTCC
GGGTGATCGTGTCGTCGGTGACCGACTGCTACACCGCACTGGGCGTCCTCCACCAGCTCTACAAGCCCGTGCCGGGCCGG
TTCAAGGACCACATCGCCATCGCCAAGCTCAACGGCTACCAATCGCTGCACACCACGCTCGTGGGCCCCTCCGGCGTCAA
CATCGAGTTCCAGATGCGCACCGAGGAGATGCACGTGGTGGCCGAGGCCGGCGTTGCGGCGCACTGGCTCTACAAGGCCG
AGAACGCCGAGGGCGGCACGGCCGAACGCCTGGGCACCAAATGGCTGCAGTCGCTGCTGGACATCCAGAACGAGACGCGC
GACGCCGCCGAATTCTGGGACCACGTGAAGGTGGACCTGTTCCCGGACGCGGTTTACGTGTTCACGCCCAAGAGCCAGAT
CATGGCCCTGCCCCGCGGCGCGACCGTCGTGGACTTCGCCTATGCCATCCACAGCAACGTGGGTGACCGGGCGACGGCCG
CGAAGATCAACAACGAGCAGGTCCCGCTGCGCACCGAGCTGAAGAACGGCGACGTGGTGGAGATCATCACCGCGCCCGTC
TCCACCCCCAACCCCGCGTGGCTGGGCTTCGTGCGCACCGGCCGCGCACGCTCGAAGATCCGCCACTACCTCAAGACCCT
CGCGCACACCGAGTCCGCCGGGCTGGGAGAGAAGCTGCTCACCCAGGCGCTGCGGGCCGAGGGCCTGGAGCGCCTGCCCT
CGGAAGAAGAGAACCAGGCCCTCTGGGACAAACTGCTGCGCTTCACCGGCAACCGCACGCGCACCGAGCTCATGACCGAC
ATCGGCCTGGGCAAGCGCATCGCAGGCATCGTGGCCAAACGGCTCATGGTGTTGATGGCCGAACACGGCCACCGCCCGGA
CGCCCTGCTGCTGACCCGTGAGCGCTATACCTCGCACGAGAACCTGTCGCAGGGCGGCGTCACGCTGGACGGCAGCGAGA
ACGCCTCCGTGCAGTACGCCAGTTGCTGCAGGCCGGTGCCGGGCGATCCGATCGTCGGCTACCTGGGCCGCGGCGAAGGA
CTCGTCGTCCACAACGCCCAATGCGGTGTGGCCAAGAAACTCCAGCACAAGGACAGCGAGCGCTTCATCACCGTCGATTG
GGCGGACGAGCCGACCCGCATGTTCGAAACCGGCATCGTGGTGACGGTCACCAACGGCAAGGGCGTGCTGGCACGCATCG
CCGCGGAACTCGCCAGCTCCGAGGCGGACATCGTGCATGTGGACATGGACGAGGAAAAGGCCATGGACACGACCGACCTG
CGCCTCGTGGTGGCCGTGCGCGACCAGGCGCACGTCGAGGCCGCGCTGCGCAACCTGCGGCGCACCACGGCCGTCCTGCG
CGCCTTCCGCATCCTGCCGTCGCCCTAG

Upstream 100 bases:

>100_bases
GACGACCGCGCATCCAGCGCGCCTCCGATCAACAGCACCGCCAGGCGGTGCTTTTTTTTGCCTTCGGCCGGCTGGGCACG
GGCACGCTACATTTCAGGGT

Downstream 100 bases:

>100_bases
CCGCACGCGGGCTGCGCCCGCCCTTTCCAAAGGAGGGCATTCCCCCACATCCCTTCGCGCCGCCAATGGGCGCAGGAAGG
GTGTCAGGAAGGCGCTCGCG

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MNEVLTKPSAPEPRPGSAPAAGASAAAANAAAASFASLTENLDYLDAASIEQVRQAYKFADKAHLGQLRSSGEPYITHPI
AVAAQCATWKLDAQALMAALLHDAMEDCGVTKADLLERFGPQVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDV
RVILIKLADRTHNMRTLSDMPRTKWGRIASETLEIYAPIAHRLGLNQTYRELQDLSFRHLHPWRYATLSKAVNKARNRRR
DLIQKVQAEVDAAFARIGMKARLAGREKTLYAIYQKMDTKHLSFAQVTDIYGFRVIVSSVTDCYTALGVLHQLYKPVPGR
FKDHIAIAKLNGYQSLHTTLVGPSGVNIEFQMRTEEMHVVAEAGVAAHWLYKAENAEGGTAERLGTKWLQSLLDIQNETR
DAAEFWDHVKVDLFPDAVYVFTPKSQIMALPRGATVVDFAYAIHSNVGDRATAAKINNEQVPLRTELKNGDVVEIITAPV
STPNPAWLGFVRTGRARSKIRHYLKTLAHTESAGLGEKLLTQALRAEGLERLPSEEENQALWDKLLRFTGNRTRTELMTD
IGLGKRIAGIVAKRLMVLMAEHGHRPDALLLTRERYTSHENLSQGGVTLDGSENASVQYASCCRPVPGDPIVGYLGRGEG
LVVHNAQCGVAKKLQHKDSERFITVDWADEPTRMFETGIVVTVTNGKGVLARIAAELASSEADIVHVDMDEEKAMDTTDL
RLVVAVRDQAHVEAALRNLRRTTAVLRAFRILPSP

Sequences:

>Translated_755_residues
MNEVLTKPSAPEPRPGSAPAAGASAAAANAAAASFASLTENLDYLDAASIEQVRQAYKFADKAHLGQLRSSGEPYITHPI
AVAAQCATWKLDAQALMAALLHDAMEDCGVTKADLLERFGPQVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDV
RVILIKLADRTHNMRTLSDMPRTKWGRIASETLEIYAPIAHRLGLNQTYRELQDLSFRHLHPWRYATLSKAVNKARNRRR
DLIQKVQAEVDAAFARIGMKARLAGREKTLYAIYQKMDTKHLSFAQVTDIYGFRVIVSSVTDCYTALGVLHQLYKPVPGR
FKDHIAIAKLNGYQSLHTTLVGPSGVNIEFQMRTEEMHVVAEAGVAAHWLYKAENAEGGTAERLGTKWLQSLLDIQNETR
DAAEFWDHVKVDLFPDAVYVFTPKSQIMALPRGATVVDFAYAIHSNVGDRATAAKINNEQVPLRTELKNGDVVEIITAPV
STPNPAWLGFVRTGRARSKIRHYLKTLAHTESAGLGEKLLTQALRAEGLERLPSEEENQALWDKLLRFTGNRTRTELMTD
IGLGKRIAGIVAKRLMVLMAEHGHRPDALLLTRERYTSHENLSQGGVTLDGSENASVQYASCCRPVPGDPIVGYLGRGEG
LVVHNAQCGVAKKLQHKDSERFITVDWADEPTRMFETGIVVTVTNGKGVLARIAAELASSEADIVHVDMDEEKAMDTTDL
RLVVAVRDQAHVEAALRNLRRTTAVLRAFRILPSP
>Mature_755_residues
MNEVLTKPSAPEPRPGSAPAAGASAAAANAAAASFASLTENLDYLDAASIEQVRQAYKFADKAHLGQLRSSGEPYITHPI
AVAAQCATWKLDAQALMAALLHDAMEDCGVTKADLLERFGPQVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDV
RVILIKLADRTHNMRTLSDMPRTKWGRIASETLEIYAPIAHRLGLNQTYRELQDLSFRHLHPWRYATLSKAVNKARNRRR
DLIQKVQAEVDAAFARIGMKARLAGREKTLYAIYQKMDTKHLSFAQVTDIYGFRVIVSSVTDCYTALGVLHQLYKPVPGR
FKDHIAIAKLNGYQSLHTTLVGPSGVNIEFQMRTEEMHVVAEAGVAAHWLYKAENAEGGTAERLGTKWLQSLLDIQNETR
DAAEFWDHVKVDLFPDAVYVFTPKSQIMALPRGATVVDFAYAIHSNVGDRATAAKINNEQVPLRTELKNGDVVEIITAPV
STPNPAWLGFVRTGRARSKIRHYLKTLAHTESAGLGEKLLTQALRAEGLERLPSEEENQALWDKLLRFTGNRTRTELMTD
IGLGKRIAGIVAKRLMVLMAEHGHRPDALLLTRERYTSHENLSQGGVTLDGSENASVQYASCCRPVPGDPIVGYLGRGEG
LVVHNAQCGVAKKLQHKDSERFITVDWADEPTRMFETGIVVTVTNGKGVLARIAAELASSEADIVHVDMDEEKAMDTTDL
RLVVAVRDQAHVEAALRNLRRTTAVLRAFRILPSP

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=718, Percent_Identity=44.8467966573816, Blast_Score=613, Evalue=1e-176,
Organism=Escherichia coli, GI1789147, Length=664, Percent_Identity=33.8855421686747, Blast_Score=367, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 83505; Mature: 83505

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEVLTKPSAPEPRPGSAPAAGASAAAANAAAASFASLTENLDYLDAASIEQVRQAYKFA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
DKAHLGQLRSSGEPYITHPIAVAAQCATWKLDAQALMAALLHDAMEDCGVTKADLLERFG
HHHHHHHHHCCCCCEEECHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHC
PQVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDVRVILIKLADRTHNMRTLSDM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCHHHHHC
PRTKWGRIASETLEIYAPIAHRLGLNQTYRELQDLSFRHLHPWRYATLSKAVNKARNRRR
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHH
DLIQKVQAEVDAAFARIGMKARLAGREKTLYAIYQKMDTKHLSFAQVTDIYGFRVIVSSV
HHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
TDCYTALGVLHQLYKPVPGRFKDHIAIAKLNGYQSLHTTLVGPSGVNIEFQMRTEEMHVV
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHEEECCCCCCEEEEEEHHHHHHH
AEAGVAAHWLYKAENAEGGTAERLGTKWLQSLLDIQNETRDAAEFWDHVKVDLFPDAVYV
HHCCHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEECCEEEE
FTPKSQIMALPRGATVVDFAYAIHSNVGDRATAAKINNEQVPLRTELKNGDVVEIITAPV
ECCHHHHEECCCCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCEEEECCCCCEEEEEECCC
STPNPAWLGFVRTGRARSKIRHYLKTLAHTESAGLGEKLLTQALRAEGLERLPSEEENQA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
LWDKLLRFTGNRTRTELMTDIGLGKRIAGIVAKRLMVLMAEHGHRPDALLLTRERYTSHE
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCC
NLSQGGVTLDGSENASVQYASCCRPVPGDPIVGYLGRGEGLVVHNAQCGVAKKLQHKDSE
CCCCCCEEECCCCCCCEEHHHHCCCCCCCCCEEEECCCCEEEEECCCCCHHHHHHCCCCC
RFITVDWADEPTRMFETGIVVTVTNGKGVLARIAAELASSEADIVHVDMDEEKAMDTTDL
CEEEEECCCCCHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCE
RLVVAVRDQAHVEAALRNLRRTTAVLRAFRILPSP
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNEVLTKPSAPEPRPGSAPAAGASAAAANAAAASFASLTENLDYLDAASIEQVRQAYKFA
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
DKAHLGQLRSSGEPYITHPIAVAAQCATWKLDAQALMAALLHDAMEDCGVTKADLLERFG
HHHHHHHHHCCCCCEEECHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHC
PQVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDVRVILIKLADRTHNMRTLSDM
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCHHHHHC
PRTKWGRIASETLEIYAPIAHRLGLNQTYRELQDLSFRHLHPWRYATLSKAVNKARNRRR
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHH
DLIQKVQAEVDAAFARIGMKARLAGREKTLYAIYQKMDTKHLSFAQVTDIYGFRVIVSSV
HHHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
TDCYTALGVLHQLYKPVPGRFKDHIAIAKLNGYQSLHTTLVGPSGVNIEFQMRTEEMHVV
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHEEECCCCCCEEEEEEHHHHHHH
AEAGVAAHWLYKAENAEGGTAERLGTKWLQSLLDIQNETRDAAEFWDHVKVDLFPDAVYV
HHCCHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEECCEEEE
FTPKSQIMALPRGATVVDFAYAIHSNVGDRATAAKINNEQVPLRTELKNGDVVEIITAPV
ECCHHHHEECCCCCHHHHHHHHHHCCCCCCHHHCCCCCCCCCEEEECCCCCEEEEEECCC
STPNPAWLGFVRTGRARSKIRHYLKTLAHTESAGLGEKLLTQALRAEGLERLPSEEENQA
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHH
LWDKLLRFTGNRTRTELMTDIGLGKRIAGIVAKRLMVLMAEHGHRPDALLLTRERYTSHE
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHCCCC
NLSQGGVTLDGSENASVQYASCCRPVPGDPIVGYLGRGEGLVVHNAQCGVAKKLQHKDSE
CCCCCCEEECCCCCCCEEHHHHCCCCCCCCCEEEECCCCEEEEECCCCCHHHHHHCCCCC
RFITVDWADEPTRMFETGIVVTVTNGKGVLARIAAELASSEADIVHVDMDEEKAMDTTDL
CEEEEECCCCCHHHHHCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCHHHCCCCCCE
RLVVAVRDQAHVEAALRNLRRTTAVLRAFRILPSP
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]