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The map label for this gene is amiC [H]

Identifier: 120612151

GI number: 120612151

Start: 3869041

End: 3870303

Strand: Reverse

Name: amiC [H]

Synonym: Aave_3500

Alternate gene names: 120612151

Gene position: 3870303-3869041 (Counterclockwise)

Preceding gene: 120612154

Following gene: 120612150

Centisome position: 72.3

GC content: 63.5

Gene sequence:

>1263_bases
ATGCAACGTCGCTTCACTCTCCAGGCGCTGTGCGCCGCAGCCGCCCTGGCAGGCCTCTCCGCGCTGCCTGCCCATGCGGC
CGACACCATCAAGGTCGGCGTGCTGCACAGCCTCTCGGGCACCATGGCCATCTCCGAAACGGTGCTCAAGGACACGGTGC
TGATGGCCATCGAGGATATCAACGCCAAGGGCGGCGTGCTGGGCAAGAAGCTCGAGCCCGTGGTGGTGGACCCGGCCTCC
AACTGGCCGCTCTTCGCCGAAAAGACCAAGCAGCTGCTGGGCCAGGACAAGGTGTCTGTGATCTTCGGCTGCTGGACCAG
CGTGAGCCGCAAGTCGGTGCTGCCCGTGGTCGAGGAAATGAACGGCCTGCTGTTCTACCCCGTGCAGTACGAAGGCGAGG
AGCTCTCCAGGAACGTGTTCTACACGGGCGCCGCGCCCAACCAGCAGGCCATTCCCGCGGTGGATTACCTCATGAGCAAG
GACGGCGGCGGTGCCAAGCGCTGGGTGCTGCTGGGCACCGACTATGTCTATCCCCGCACCACCAACAAGATCCTGCGCGC
CTACCTGAAGTCCAAGGGCGTGAAGGATTCGGACATCGACGAGAAGTACACCCCCTTCGGCCACTCCGACTACCAGACCA
TCGTGGCCGACATCAGGAAGTTCAGCCAGGGCGGCAAGACGGCCGTGGTCTCCACCATCAACGGGGATTCCAACGTGCCC
TTCTACAAGGAACTGGGCAACGCGGGCCTGAAGGCCAAGGACGTGCCGGTGGTGGCCTTCAGCGTGGGCGAGGAAGAACT
GCGCGGCGTGGACACCAAGCCGCTCGTGGGCCACCTGGCCGCATGGAACTACTTCATGTCCCTGAAGAACCCGGAGAACA
CCGCCTTCATCCAGCAGTGGAGCGCCTACGCCAAGAAGAACAACATCCCGGGCCACAAGGACAAGCCGCTGACCAACGAC
CCGATGGAGGCGACCTGGATCGGCATCCACATGTGGAAGCAGGCCGTGGAGAAGGCCAAGAGCACCGACGTGGACAAGGT
GATCGCCGCCATGGCCGGCCAGACCTTCAAGGCGCCCTCGGGCATCGTCAGCAAGATGGACGAGAAGAACCACCACCTGC
ACAAGAGCGTGTTCATCGGCGAGATCAAGGCCGACGGCCAGTTCAACGTGGTGTGGAAGACGCCCGGCCCGGTCAAGGCC
AAGCCGTGGAGCCCGTACATCGAGGGCAACGACAAGAAGAAGGACGAGCCCCTGACCAGGTAA

Upstream 100 bases:

>100_bases
GCCCCTTGGCGTATTTCGCGGCCGGGGCGGCCTTCGCAGAATCGGTTCCAGCGATTCCCCGACGACCCCGTCCTTCACTT
CCTTCCCGGAGCCCTCACCC

Downstream 100 bases:

>100_bases
CACCCCCCTGAGGCGCTGCGCGCCTTCCCCCCTCTCTCACGCTGCGCGTGGGAGGGGGGACGACGCCAGCGGCCCGGCGG
AGCCGGTTCCGCGGCGTCTG

Product: twin-arginine translocation pathway signal protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MQRRFTLQALCAAAALAGLSALPAHAADTIKVGVLHSLSGTMAISETVLKDTVLMAIEDINAKGGVLGKKLEPVVVDPAS
NWPLFAEKTKQLLGQDKVSVIFGCWTSVSRKSVLPVVEEMNGLLFYPVQYEGEELSRNVFYTGAAPNQQAIPAVDYLMSK
DGGGAKRWVLLGTDYVYPRTTNKILRAYLKSKGVKDSDIDEKYTPFGHSDYQTIVADIRKFSQGGKTAVVSTINGDSNVP
FYKELGNAGLKAKDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMSLKNPENTAFIQQWSAYAKKNNIPGHKDKPLTND
PMEATWIGIHMWKQAVEKAKSTDVDKVIAAMAGQTFKAPSGIVSKMDEKNHHLHKSVFIGEIKADGQFNVVWKTPGPVKA
KPWSPYIEGNDKKKDEPLTR

Sequences:

>Translated_420_residues
MQRRFTLQALCAAAALAGLSALPAHAADTIKVGVLHSLSGTMAISETVLKDTVLMAIEDINAKGGVLGKKLEPVVVDPAS
NWPLFAEKTKQLLGQDKVSVIFGCWTSVSRKSVLPVVEEMNGLLFYPVQYEGEELSRNVFYTGAAPNQQAIPAVDYLMSK
DGGGAKRWVLLGTDYVYPRTTNKILRAYLKSKGVKDSDIDEKYTPFGHSDYQTIVADIRKFSQGGKTAVVSTINGDSNVP
FYKELGNAGLKAKDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMSLKNPENTAFIQQWSAYAKKNNIPGHKDKPLTND
PMEATWIGIHMWKQAVEKAKSTDVDKVIAAMAGQTFKAPSGIVSKMDEKNHHLHKSVFIGEIKADGQFNVVWKTPGPVKA
KPWSPYIEGNDKKKDEPLTR
>Mature_420_residues
MQRRFTLQALCAAAALAGLSALPAHAADTIKVGVLHSLSGTMAISETVLKDTVLMAIEDINAKGGVLGKKLEPVVVDPAS
NWPLFAEKTKQLLGQDKVSVIFGCWTSVSRKSVLPVVEEMNGLLFYPVQYEGEELSRNVFYTGAAPNQQAIPAVDYLMSK
DGGGAKRWVLLGTDYVYPRTTNKILRAYLKSKGVKDSDIDEKYTPFGHSDYQTIVADIRKFSQGGKTAVVSTINGDSNVP
FYKELGNAGLKAKDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMSLKNPENTAFIQQWSAYAKKNNIPGHKDKPLTND
PMEATWIGIHMWKQAVEKAKSTDVDKVIAAMAGQTFKAPSGIVSKMDEKNHHLHKSVFIGEIKADGQFNVVWKTPGPVKA
KPWSPYIEGNDKKKDEPLTR

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46008; Mature: 46008

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRFTLQALCAAAALAGLSALPAHAADTIKVGVLHSLSGTMAISETVLKDTVLMAIEDI
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHECC
NAKGGVLGKKLEPVVVDPASNWPLFAEKTKQLLGQDKVSVIFGCWTSVSRKSVLPVVEEM
CCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCEEEEEEEHHHCCHHHHHHHHHHC
NGLLFYPVQYEGEELSRNVFYTGAAPNQQAIPAVDYLMSKDGGGAKRWVLLGTDYVYPRT
CCEEEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEEEEECCEECCCC
TNKILRAYLKSKGVKDSDIDEKYTPFGHSDYQTIVADIRKFSQGGKTAVVSTINGDSNVP
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
FYKELGNAGLKAKDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMSLKNPENTAFIQQW
HHHHHCCCCCCCCCCCEEEEECCHHHHCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHH
SAYAKKNNIPGHKDKPLTNDPMEATWIGIHMWKQAVEKAKSTDVDKVIAAMAGQTFKAPS
HHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCH
GIVSKMDEKNHHLHKSVFIGEIKADGQFNVVWKTPGPVKAKPWSPYIEGNDKKKDEPLTR
HHHHHHHHHCCHHHHEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQRRFTLQALCAAAALAGLSALPAHAADTIKVGVLHSLSGTMAISETVLKDTVLMAIEDI
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHECC
NAKGGVLGKKLEPVVVDPASNWPLFAEKTKQLLGQDKVSVIFGCWTSVSRKSVLPVVEEM
CCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCEEEEEEEHHHCCHHHHHHHHHHC
NGLLFYPVQYEGEELSRNVFYTGAAPNQQAIPAVDYLMSKDGGGAKRWVLLGTDYVYPRT
CCEEEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEEEEECCEECCCC
TNKILRAYLKSKGVKDSDIDEKYTPFGHSDYQTIVADIRKFSQGGKTAVVSTINGDSNVP
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCC
FYKELGNAGLKAKDVPVVAFSVGEEELRGVDTKPLVGHLAAWNYFMSLKNPENTAFIQQW
HHHHHCCCCCCCCCCCEEEEECCHHHHCCCCCCHHHHHHHHHHHHEECCCCCCHHHHHHH
SAYAKKNNIPGHKDKPLTNDPMEATWIGIHMWKQAVEKAKSTDVDKVIAAMAGQTFKAPS
HHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCH
GIVSKMDEKNHHLHKSVFIGEIKADGQFNVVWKTPGPVKAKPWSPYIEGNDKKKDEPLTR
HHHHHHHHHCCHHHHEEEEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]