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The map label for this gene is holB [H]

Identifier: 120612064

GI number: 120612064

Start: 3767255

End: 3768256

Strand: Reverse

Name: holB [H]

Synonym: Aave_3411

Alternate gene names: 120612064

Gene position: 3768256-3767255 (Counterclockwise)

Preceding gene: 120612065

Following gene: 120612063

Centisome position: 70.4

GC content: 73.25

Gene sequence:

>1002_bases
ATGAGCGCGGCCGCCGTGTCTCCCTGGATCGCTGCCCAGCGCCGCAGGCTCCTGGCCCAGCGCGGCCATGCCTGGCTGCT
CAGCGGCCCCTCCGGGCTGGGGCAGTACACGCTCGCGCTCGAACTGGTGCGTGCCTGGCTGTGCGAAGCGCCTGCCGAAG
ACGGCGCATGCGGGCACTGCGGCAGCTGCCATGCCATCGACGTGCGCACCCATGCCGACCTCTGCGTGCTCATGCCCGAG
ACGGAGATGATCGAGCGCGGATGGCCGCTGCCCGAGAAGGCGCAGGCCGAGATCGACGACAAGAAGCGCAAGGCCAGCCG
CGAGATCCGCGTCGAGGCGATGCGCGACGCGGTGCAGTTCGCGCAGCGCACTTCCGCGCGCGGACGGGGCAAGGCCGTGC
TCGTCTATCCGGCCGAGCAGATGAACACCGTCACCGCCAACGCCCTGCTCAAGACGCTGGAGGAGCCCGTGGGCGACGTG
CGCTTCGTGCTGGCGAGCGAGGCCGCGCACCAGTTGCTGCCCACCATCCGCAGCCGCTGCCTGGGCCACACGATGCAGTG
GCCCGGCGAGGAAGAGTCGCTGGCCTGGCTCGCGGGGCAGGGCGTCGCGGCCGATGCCGCGGCATCCCTGCTGCGGGCGG
CCGGAGGCCGGCCCGAGGACGCCCTGGCCTTCGCCCGGGGAGGGCGCAGCCCCCAGGCCTGGTCGCAGATTCCGCGCGCC
GTGGCCCGCGGCGACGCAACGGCGCTGTCGGACCTGTCGCCCGCCGACGCCGTGGACGCCCTGCAGAAGCTCTGCCACGA
CCTGCTCGCCCGGCGCGTGGGCGCCGCACCCCGCTACTTCGCCGAGGCCGACCTGCCTTCGCCGCCCGGCGTCGGCCCGC
TCACCCGCTGGGCCCGCGCGCTCGCGAAGGAGGCCCGCACCGCGGACCATCCCTTCAATCCGGGATTGATGCTGGAGTCG
CTTGTTGCCCAGGCGCGAAACGCCCTAAACTCCCGCCATTGA

Upstream 100 bases:

>100_bases
TCGCGCGGCTCGATGCCGACCAGCCGCGCGAGGCGGTGCGTGCGCAGCTCATGGAAATCCTCCACCGGCGTGGCGTGCTG
GATGCGGCGCAGGAGGGCGG

Downstream 100 bases:

>100_bases
ACGACCGGCCCGTCCCCACATGAGTACCCCTCCCAGCGCCCCCCGTCCCAGCGTCATGCAGCTGGCCATCAAGGAAAAGG
CGGCACTCTACGCGGCGTAC

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MSAAAVSPWIAAQRRRLLAQRGHAWLLSGPSGLGQYTLALELVRAWLCEAPAEDGACGHCGSCHAIDVRTHADLCVLMPE
TEMIERGWPLPEKAQAEIDDKKRKASREIRVEAMRDAVQFAQRTSARGRGKAVLVYPAEQMNTVTANALLKTLEEPVGDV
RFVLASEAAHQLLPTIRSRCLGHTMQWPGEEESLAWLAGQGVAADAAASLLRAAGGRPEDALAFARGGRSPQAWSQIPRA
VARGDATALSDLSPADAVDALQKLCHDLLARRVGAAPRYFAEADLPSPPGVGPLTRWARALAKEARTADHPFNPGLMLES
LVAQARNALNSRH

Sequences:

>Translated_333_residues
MSAAAVSPWIAAQRRRLLAQRGHAWLLSGPSGLGQYTLALELVRAWLCEAPAEDGACGHCGSCHAIDVRTHADLCVLMPE
TEMIERGWPLPEKAQAEIDDKKRKASREIRVEAMRDAVQFAQRTSARGRGKAVLVYPAEQMNTVTANALLKTLEEPVGDV
RFVLASEAAHQLLPTIRSRCLGHTMQWPGEEESLAWLAGQGVAADAAASLLRAAGGRPEDALAFARGGRSPQAWSQIPRA
VARGDATALSDLSPADAVDALQKLCHDLLARRVGAAPRYFAEADLPSPPGVGPLTRWARALAKEARTADHPFNPGLMLES
LVAQARNALNSRH
>Mature_332_residues
SAAAVSPWIAAQRRRLLAQRGHAWLLSGPSGLGQYTLALELVRAWLCEAPAEDGACGHCGSCHAIDVRTHADLCVLMPET
EMIERGWPLPEKAQAEIDDKKRKASREIRVEAMRDAVQFAQRTSARGRGKAVLVYPAEQMNTVTANALLKTLEEPVGDVR
FVLASEAAHQLLPTIRSRCLGHTMQWPGEEESLAWLAGQGVAADAAASLLRAAGGRPEDALAFARGGRSPQAWSQIPRAV
ARGDATALSDLSPADAVDALQKLCHDLLARRVGAAPRYFAEADLPSPPGVGPLTRWARALAKEARTADHPFNPGLMLESL
VAQARNALNSRH

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=226, Percent_Identity=32.3008849557522, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786676, Length=226, Percent_Identity=31.4159292035398, Blast_Score=81, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35794; Mature: 35663

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAAVSPWIAAQRRRLLAQRGHAWLLSGPSGLGQYTLALELVRAWLCEAPAEDGACGHC
CCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
GSCHAIDVRTHADLCVLMPETEMIERGWPLPEKAQAEIDDKKRKASREIRVEAMRDAVQF
CCCCEEEEECCCCEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRTSARGRGKAVLVYPAEQMNTVTANALLKTLEEPVGDVRFVLASEAAHQLLPTIRSRC
HHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGHTMQWPGEEESLAWLAGQGVAADAAASLLRAAGGRPEDALAFARGGRSPQAWSQIPRA
HCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHH
VARGDATALSDLSPADAVDALQKLCHDLLARRVGAAPRYFAEADLPSPPGVGPLTRWARA
HHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHHH
LAKEARTADHPFNPGLMLESLVAQARNALNSRH
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAAAVSPWIAAQRRRLLAQRGHAWLLSGPSGLGQYTLALELVRAWLCEAPAEDGACGHC
CCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
GSCHAIDVRTHADLCVLMPETEMIERGWPLPEKAQAEIDDKKRKASREIRVEAMRDAVQF
CCCCEEEEECCCCEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRTSARGRGKAVLVYPAEQMNTVTANALLKTLEEPVGDVRFVLASEAAHQLLPTIRSRC
HHHHCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGHTMQWPGEEESLAWLAGQGVAADAAASLLRAAGGRPEDALAFARGGRSPQAWSQIPRA
HCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHH
VARGDATALSDLSPADAVDALQKLCHDLLARRVGAAPRYFAEADLPSPPGVGPLTRWARA
HHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCHHHHHHHH
LAKEARTADHPFNPGLMLESLVAQARNALNSRH
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]