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The map label for this gene is smpB

Identifier: 120612028

GI number: 120612028

Start: 3728672

End: 3729145

Strand: Direct

Name: smpB

Synonym: Aave_3374

Alternate gene names: 120612028

Gene position: 3728672-3729145 (Clockwise)

Preceding gene: 120612025

Following gene: 120612029

Centisome position: 69.66

GC content: 64.14

Gene sequence:

>474_bases
ATGGCCAAGAAACCCGAGACCCTGTCCCGCATCGCCGACAACAAGAAGGCGGCCTTCAACTATTTCTTCGAGGAACGCCA
CGAGGCGGGCATGGTGCTGCACGGCTGGGAGGTCAAGGCCCTGCGCGAGGGCAAGGTGCAGCTCACCGACGGCTACGTGG
TGATCCGCGAAGGCGAGCTCTACCTGATCGGCTGCCAGATCAATCCCCTGAAGACGGCCTCCACCCACGTGAGTCCGGAT
GCGGTCCGCACCAAGAAGCTGCTGCTGCACAAGGACGAGATCAAGCGCCTTACGGCCAAGGTGGAGCAGAAGGGCTACAC
GCTGGTGCCGTTGAACCTGCACTGGACCAATGGCCGCGCCAAGTGCGAGATCGCGCTCGCCAAGGGCAAGGCCGAGCACG
ACAAGCGCGACACCATCAAGGAGCGCGAGGGCAAGCGCGAGGTGGAGCGCGCCATGAAGAGCCGCCACCGCTGA

Upstream 100 bases:

>100_bases
GTGGCGGGACGGAGCACTAGAATCCCCGGATAGCGCACGGCCGATCGTCCGATTGTAGGGAGGGCTTTCAAACGCCTCCA
TGTGCCGCATCTGATTCCCC

Downstream 100 bases:

>100_bases
CGCGGCAGGAAATTTTTTCGGGCGGCGCGGAATAAATCCGCCGCGCCCGGTGTTCATTCCATCACGGGCCCGGCATGTCG
CCGCCGCCCGTGCCTACAGG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MAKKPETLSRIADNKKAAFNYFFEERHEAGMVLHGWEVKALREGKVQLTDGYVVIREGELYLIGCQINPLKTASTHVSPD
AVRTKKLLLHKDEIKRLTAKVEQKGYTLVPLNLHWTNGRAKCEIALAKGKAEHDKRDTIKEREGKREVERAMKSRHR

Sequences:

>Translated_157_residues
MAKKPETLSRIADNKKAAFNYFFEERHEAGMVLHGWEVKALREGKVQLTDGYVVIREGELYLIGCQINPLKTASTHVSPD
AVRTKKLLLHKDEIKRLTAKVEQKGYTLVPLNLHWTNGRAKCEIALAKGKAEHDKRDTIKEREGKREVERAMKSRHR
>Mature_156_residues
AKKPETLSRIADNKKAAFNYFFEERHEAGMVLHGWEVKALREGKVQLTDGYVVIREGELYLIGCQINPLKTASTHVSPDA
VRTKKLLLHKDEIKRLTAKVEQKGYTLVPLNLHWTNGRAKCEIALAKGKAEHDKRDTIKEREGKREVERAMKSRHR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=156, Percent_Identity=50, Blast_Score=157, Evalue=3e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_ACIAC (A1TSJ4)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971706.1
- ProteinModelPortal:   A1TSJ4
- SMR:   A1TSJ4
- STRING:   A1TSJ4
- GeneID:   4666391
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3374
- NMPDR:   fig|397945.5.peg.2881
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- PhylomeDB:   A1TSJ4
- ProtClustDB:   PRK05422
- BioCyc:   AAVE397945:AAVE_3374-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18043; Mature: 17912

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKPETLSRIADNKKAAFNYFFEERHEAGMVLHGWEVKALREGKVQLTDGYVVIREGEL
CCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECEEEEEECCCCEEEECCEEEEECCCE
YLIGCQINPLKTASTHVSPDAVRTKKLLLHKDEIKRLTAKVEQKGYTLVPLNLHWTNGRA
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCE
KCEIALAKGKAEHDKRDTIKEREGKREVERAMKSRHR
EEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AKKPETLSRIADNKKAAFNYFFEERHEAGMVLHGWEVKALREGKVQLTDGYVVIREGEL
CCCHHHHHHHHCCCHHHHHHHHHHHCCCCEEEECEEEEEECCCCEEEECCEEEEECCCE
YLIGCQINPLKTASTHVSPDAVRTKKLLLHKDEIKRLTAKVEQKGYTLVPLNLHWTNGRA
EEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCE
KCEIALAKGKAEHDKRDTIKEREGKREVERAMKSRHR
EEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA