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The map label for this gene is hsdS [H]

Identifier: 120612012

GI number: 120612012

Start: 3713465

End: 3714754

Strand: Reverse

Name: hsdS [H]

Synonym: Aave_3358

Alternate gene names: 120612012

Gene position: 3714754-3713465 (Counterclockwise)

Preceding gene: 120612013

Following gene: 120612011

Centisome position: 69.4

GC content: 58.14

Gene sequence:

>1290_bases
GTGAACACATCGACTGTGCGAGTCGATCCTGAAGTGTGGCGATTGGCACGCTTGAAATTCGTTGCACCTTTACGAAACGA
ACGCATGTCCGCCGGTTCCGACCATCCCGGCTACCTTGGCCTTGAAAACATCGAATCGTGGACGGGCAGGATCATCGAGG
TCGAATCGAAGCGGGATGACGAGCCCGCCGACCAAAGCGCGGGGCTTGCCAATATATTCAGGGAAGGCGATGTGCTGTTT
TGCAAACTTCGCCCTTATTTGGCCAAGGCGTGTCACGCACCGCGTGATGGTGTCGGCTCGACCGAGTTGCTCGTCATGCG
GCCTTCGGAGCTTCTCGAACCACGCTTCCTGCTCTACTCCATCTTGACGCCAGATTTCGTTGGTGCCGTTGATGCGTCAA
CCTTCGGCGCCAAAATGCCGCGTGCCAACTGGGATTTCATCGGATCGCTGGAAGTCAAAGTTCCACCACTCGAAGAACAG
CGCCTTATCGCCAACTACCTCGACCGCGAAACCGCCGGCATCGACGGACTGATCGCCGAGAAGGAGCGTATGCTGGCGCT
ACTGGAAGAAAAGCGCGCCGCTCTCATCAGCCGTGTCGTCACCCGCGGCCTTGACCCCAACGCCCCGCTCAAACCCTCCG
GCCAGGAATGGTTGGGCGAGATTCCGGTGCATTGGGGTTTGCAACGCCTAAAGCAGCTTGCCGAGGTTCGAGGTGGATTG
ACACTGGGCAAGCAGTACTCCGGCGAATTGTTGGAGTACCCCTACCTACGTGTCGCAAACGTACAGGATGGGTACCTTAA
GCTGGACGACGTACTGACAGTTGAGGTGCCCGCCTCAGAGGCCGCATCGAACCTTCTGGTATATGGCGATGTGTTGATGA
ACGAGGGGGGTGACATCGACAAGCTCGGCAGGGGCTGTGTTTGGCGAGACGAGATTTCGCCTTGCCTCCATCAGAACCAC
GTCTTTGCTGTCCGGCCACATTCTGTTGATTCCGATTGGCTCGCGCTTTGGACATCGACGATCCAGGCAAAGCGCTATTT
CGAGAGCCGCGCTAAGCGGTCGACCAATCTTGCATCCATATCTGGATCGAACATCAAGGAGCTTCCCGTTCCCTTGCCAC
CGGTAAGCGAGCAGCTCGCAATCCAAAATTTCCTGGCAGTCAGGCATTCGCGGCTTGAGACGCTCCGCGGCGAACTGCGA
GACTCGCTGCGCCTGCTTATCGAACGCCGCGCGGCGCTGATCACGGCCGGAGTTACCGGCCAGATTCCGCTGGAGGAGCT
AGCCGGATGA

Upstream 100 bases:

>100_bases
TGTTCTTCCAGTACCAGCCGCCACGCCCGCTGCATGAGATCGATGCGGAGCTTGCGGAAGTTGAGAAGCGGATTCTCGAT
TTGCTGCGGGAGGTGACGGA

Downstream 100 bases:

>100_bases
AGCTGGAATCCCTATCCCTCGCGCACTGCGGCGGCTTCGAGCAGCTCGACATCGCTTTCGAGCCGGATGTGACGCTGATC
GCGGGTGTCAACGGCGTAGG

Product: restriction modification system DNA specificity subunit

Products: NA

Alternate protein names: S.StySJI; Type I restriction enzyme StySJI specificity protein; S protein [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNTSTVRVDPEVWRLARLKFVAPLRNERMSAGSDHPGYLGLENIESWTGRIIEVESKRDDEPADQSAGLANIFREGDVLF
CKLRPYLAKACHAPRDGVGSTELLVMRPSELLEPRFLLYSILTPDFVGAVDASTFGAKMPRANWDFIGSLEVKVPPLEEQ
RLIANYLDRETAGIDGLIAEKERMLALLEEKRAALISRVVTRGLDPNAPLKPSGQEWLGEIPVHWGLQRLKQLAEVRGGL
TLGKQYSGELLEYPYLRVANVQDGYLKLDDVLTVEVPASEAASNLLVYGDVLMNEGGDIDKLGRGCVWRDEISPCLHQNH
VFAVRPHSVDSDWLALWTSTIQAKRYFESRAKRSTNLASISGSNIKELPVPLPPVSEQLAIQNFLAVRHSRLETLRGELR
DSLRLLIERRAALITAGVTGQIPLEELAG

Sequences:

>Translated_429_residues
MNTSTVRVDPEVWRLARLKFVAPLRNERMSAGSDHPGYLGLENIESWTGRIIEVESKRDDEPADQSAGLANIFREGDVLF
CKLRPYLAKACHAPRDGVGSTELLVMRPSELLEPRFLLYSILTPDFVGAVDASTFGAKMPRANWDFIGSLEVKVPPLEEQ
RLIANYLDRETAGIDGLIAEKERMLALLEEKRAALISRVVTRGLDPNAPLKPSGQEWLGEIPVHWGLQRLKQLAEVRGGL
TLGKQYSGELLEYPYLRVANVQDGYLKLDDVLTVEVPASEAASNLLVYGDVLMNEGGDIDKLGRGCVWRDEISPCLHQNH
VFAVRPHSVDSDWLALWTSTIQAKRYFESRAKRSTNLASISGSNIKELPVPLPPVSEQLAIQNFLAVRHSRLETLRGELR
DSLRLLIERRAALITAGVTGQIPLEELAG
>Mature_429_residues
MNTSTVRVDPEVWRLARLKFVAPLRNERMSAGSDHPGYLGLENIESWTGRIIEVESKRDDEPADQSAGLANIFREGDVLF
CKLRPYLAKACHAPRDGVGSTELLVMRPSELLEPRFLLYSILTPDFVGAVDASTFGAKMPRANWDFIGSLEVKVPPLEEQ
RLIANYLDRETAGIDGLIAEKERMLALLEEKRAALISRVVTRGLDPNAPLKPSGQEWLGEIPVHWGLQRLKQLAEVRGGL
TLGKQYSGELLEYPYLRVANVQDGYLKLDDVLTVEVPASEAASNLLVYGDVLMNEGGDIDKLGRGCVWRDEISPCLHQNH
VFAVRPHSVDSDWLALWTSTIQAKRYFESRAKRSTNLASISGSNIKELPVPLPPVSEQLAIQNFLAVRHSRLETLRGELR
DSLRLLIERRAALITAGVTGQIPLEELAG

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=312, Percent_Identity=24.3589743589744, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 47713; Mature: 47713

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSTVRVDPEVWRLARLKFVAPLRNERMSAGSDHPGYLGLENIESWTGRIIEVESKRDD
CCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCC
EPADQSAGLANIFREGDVLFCKLRPYLAKACHAPRDGVGSTELLVMRPSELLEPRFLLYS
CCCCCCCCHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCCEEEEECCHHHCCHHHHHHH
ILTPDFVGAVDASTFGAKMPRANWDFIGSLEVKVPPLEEQRLIANYLDRETAGIDGLIAE
HHCCHHHCCCCCHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHH
KERMLALLEEKRAALISRVVTRGLDPNAPLKPSGQEWLGEIPVHWGLQRLKQLAEVRGGL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCC
TLGKQYSGELLEYPYLRVANVQDGYLKLDDVLTVEVPASEAASNLLVYGDVLMNEGGDID
EECCCCCCCHHCCCCEEEECCCCCCEEECCEEEEEECCHHHHCCEEEEEHHHHCCCCCHH
KLGRGCVWRDEISPCLHQNHVFAVRPHSVDSDWLALWTSTIQAKRYFESRAKRSTNLASI
HHCCCCEEHHHHCHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
SGSNIKELPVPLPPVSEQLAIQNFLAVRHSRLETLRGELRDSLRLLIERRAALITAGVTG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
QIPLEELAG
CCCHHHHCC
>Mature Secondary Structure
MNTSTVRVDPEVWRLARLKFVAPLRNERMSAGSDHPGYLGLENIESWTGRIIEVESKRDD
CCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCC
EPADQSAGLANIFREGDVLFCKLRPYLAKACHAPRDGVGSTELLVMRPSELLEPRFLLYS
CCCCCCCCHHHHHCCCCEEEEECCHHHHHHHCCCCCCCCCCEEEEECCHHHCCHHHHHHH
ILTPDFVGAVDASTFGAKMPRANWDFIGSLEVKVPPLEEQRLIANYLDRETAGIDGLIAE
HHCCHHHCCCCCHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHH
KERMLALLEEKRAALISRVVTRGLDPNAPLKPSGQEWLGEIPVHWGLQRLKQLAEVRGGL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCC
TLGKQYSGELLEYPYLRVANVQDGYLKLDDVLTVEVPASEAASNLLVYGDVLMNEGGDID
EECCCCCCCHHCCCCEEEECCCCCCEEECCEEEEEECCHHHHCCEEEEEHHHHCCCCCHH
KLGRGCVWRDEISPCLHQNHVFAVRPHSVDSDWLALWTSTIQAKRYFESRAKRSTNLASI
HHCCCCEEHHHHCHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
SGSNIKELPVPLPPVSEQLAIQNFLAVRHSRLETLRGELRDSLRLLIERRAALITAGVTG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
QIPLEELAG
CCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2838725; 11677609; 3025838 [H]