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The map label for this gene is rumA

Identifier: 120611974

GI number: 120611974

Start: 3667486

End: 3668961

Strand: Reverse

Name: rumA

Synonym: Aave_3318

Alternate gene names: 120611974

Gene position: 3668961-3667486 (Counterclockwise)

Preceding gene: 120611975

Following gene: 120611971

Centisome position: 68.54

GC content: 69.31

Gene sequence:

>1476_bases
ATGAGCGACCCCACAGAACATCCCGAGATCCTCCAGGACCCATCTTCCTCCGCGCCCGTGCAGGGGCGTACCGACCTCCC
GCCCGGCTGGCTGGAAGTCACCTCCATGGACATGGACGCCCAGGGCGTGGCCCGCAGGCCCGACGGCAAGGTCGTCTTCA
TCGATGGCGCACTGCCCTTCGAATGGGTGAGTGCCAGCACCCACCGCAAGAAGAACAACTGGGAGCAGGCGAGCCTCACC
GCCATCCACCGCGAATCCCCGCAGCGCGTGCGCCCGGGGTGTCCCCATTTCGGGCTGCATGCCGGCGCCTGCGGCGGCTG
CAAGATGCAGCATCTGCACGTCGGCGCCCAGGTGGCCGTCAAGCAGCGCGTGCTGGAAGACAACCTCTGGCACCTGGGCA
AGGTGAAGGCGGAAACCGTGCTGCGGCCCATCGAAGGGCCTGCCTGGGGCTATCGCTACCGCGCGCGCCTGGCCGTGCGG
CACGTCGAGAAAAAGGGCAAGGTGCTGGTGGGTTTCCACGAGCGCAAGAGCCGCTACATCGCCGACATGGAAGTCTGCCC
GGTGCTGCCGCCCCATGTGAGCGCCATGCTGCTGCCCTTGCGCGCACTGATCGCGTCCATGGACGCACGCGACACCTGCC
CGCAGATCGAACTGGCCTGCGGCGATGACGTCACCGCGCTGGTGCTGCGCCACCTGGAGCCGCTGTCCGAAGCCGACCTG
GGCCGGCTGCGCTCCTTCGCCGCCGCGCATGGCGTGCAGTGGTGGCTGCAGCCCAAGGGTCCGGACACCGTGCACCTGCT
GGACGAGGGCGGCCCGCAACTGAACTACGCTTTGCCGGATTTCGGCATCACCATGCCCTTCAAGCCCACCGACTTCACGC
AGGTCAACCCGCACATCAACCGCGTGCTGGTCACGCGTGCGCTGCGCCTGCTGGATGCGGGCCGCACCGACCGGGTGATC
GACTGGTTCTGCGGCCTGGGCAACTTCACGCTGCCCATCGCCACGCAGGCCCGCGAAGTCGTCGGCATCGAGGGCAGCGA
GGCGCTGGTGGCCCGCTCGCGCGAGAACTACCGCAAGAACCAGGACGACCGGCCGCAGGGGCAGGCGCTGGCCCCCGCCA
CGTTCGTGGCGCGCAACCTGTTCGAGATGACGCCCGAAATGCTCATCGCCGACGGCGTGGCCGACAAATGGCTGGTCGAT
CCACCGCGCGAGGGCGCGTTCGCGCTCGCCAAGGCCCTGGCCGACATCCACCAGGCGCGCATCGGCGCGGAAGATGCCCC
GGCGCTGCCCGCGTCGGCCGAAGGCTGGACGCCGCCGCAGCGCATCGTCTACGTGAGCTGCAATCCGGCCACGCTGGCGC
GCGACGCGGGCCTGCTGGTGCACCAGGCGGGCTACCGCTGCGTGGCGGCCGGCGTGGTGAACATGTTCCCCCACACGGCG
CATGTCGAGAGCATGGCGGTGTTCGAACGCGTGTGA

Upstream 100 bases:

>100_bases
GGCTCCCGCGTGGGCCTGCCGCCTTCCGATCCCGTGCCGCGGCTGGTACGCCCTGCGCGCAGCTGACCGGCCACTGCGGC
CGGCCTGAGACAATCGGGGG

Downstream 100 bases:

>100_bases
GATCGGAAATGCGTTCCCGGAACCGGGTGCTGCTGCTTGAGTGGCCCAACGGCAGGCCTTGAAGGGTTGGCCGGGCCGGC
CGGCGGGCCTGCAGTGCGAA

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSDPTEHPEILQDPSSSAPVQGRTDLPPGWLEVTSMDMDAQGVARRPDGKVVFIDGALPFEWVSASTHRKKNNWEQASLT
AIHRESPQRVRPGCPHFGLHAGACGGCKMQHLHVGAQVAVKQRVLEDNLWHLGKVKAETVLRPIEGPAWGYRYRARLAVR
HVEKKGKVLVGFHERKSRYIADMEVCPVLPPHVSAMLLPLRALIASMDARDTCPQIELACGDDVTALVLRHLEPLSEADL
GRLRSFAAAHGVQWWLQPKGPDTVHLLDEGGPQLNYALPDFGITMPFKPTDFTQVNPHINRVLVTRALRLLDAGRTDRVI
DWFCGLGNFTLPIATQAREVVGIEGSEALVARSRENYRKNQDDRPQGQALAPATFVARNLFEMTPEMLIADGVADKWLVD
PPREGAFALAKALADIHQARIGAEDAPALPASAEGWTPPQRIVYVSCNPATLARDAGLLVHQAGYRCVAAGVVNMFPHTA
HVESMAVFERV

Sequences:

>Translated_491_residues
MSDPTEHPEILQDPSSSAPVQGRTDLPPGWLEVTSMDMDAQGVARRPDGKVVFIDGALPFEWVSASTHRKKNNWEQASLT
AIHRESPQRVRPGCPHFGLHAGACGGCKMQHLHVGAQVAVKQRVLEDNLWHLGKVKAETVLRPIEGPAWGYRYRARLAVR
HVEKKGKVLVGFHERKSRYIADMEVCPVLPPHVSAMLLPLRALIASMDARDTCPQIELACGDDVTALVLRHLEPLSEADL
GRLRSFAAAHGVQWWLQPKGPDTVHLLDEGGPQLNYALPDFGITMPFKPTDFTQVNPHINRVLVTRALRLLDAGRTDRVI
DWFCGLGNFTLPIATQAREVVGIEGSEALVARSRENYRKNQDDRPQGQALAPATFVARNLFEMTPEMLIADGVADKWLVD
PPREGAFALAKALADIHQARIGAEDAPALPASAEGWTPPQRIVYVSCNPATLARDAGLLVHQAGYRCVAAGVVNMFPHTA
HVESMAVFERV
>Mature_490_residues
SDPTEHPEILQDPSSSAPVQGRTDLPPGWLEVTSMDMDAQGVARRPDGKVVFIDGALPFEWVSASTHRKKNNWEQASLTA
IHRESPQRVRPGCPHFGLHAGACGGCKMQHLHVGAQVAVKQRVLEDNLWHLGKVKAETVLRPIEGPAWGYRYRARLAVRH
VEKKGKVLVGFHERKSRYIADMEVCPVLPPHVSAMLLPLRALIASMDARDTCPQIELACGDDVTALVLRHLEPLSEADLG
RLRSFAAAHGVQWWLQPKGPDTVHLLDEGGPQLNYALPDFGITMPFKPTDFTQVNPHINRVLVTRALRLLDAGRTDRVID
WFCGLGNFTLPIATQAREVVGIEGSEALVARSRENYRKNQDDRPQGQALAPATFVARNLFEMTPEMLIADGVADKWLVDP
PREGAFALAKALADIHQARIGAEDAPALPASAEGWTPPQRIVYVSCNPATLARDAGLLVHQAGYRCVAAGVVNMFPHTAH
VESMAVFERV

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Escherichia coli, GI1789148, Length=461, Percent_Identity=38.6117136659436, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1787083, Length=413, Percent_Identity=25.4237288135593, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_ACIAC (A1TSE0)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_971652.1
- ProteinModelPortal:   A1TSE0
- SMR:   A1TSE0
- STRING:   A1TSE0
- GeneID:   4668187
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_3318
- NMPDR:   fig|397945.5.peg.2836
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   VVFVEGA
- PhylomeDB:   A1TSE0
- ProtClustDB:   PRK13168
- BioCyc:   AAVE397945:AAVE_3318-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 53960; Mature: 53829

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 447-447 BINDING 294-294 BINDING 323-323 BINDING 328-328 BINDING 344-344 BINDING 379-379 BINDING 400-400

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPTEHPEILQDPSSSAPVQGRTDLPPGWLEVTSMDMDAQGVARRPDGKVVFIDGALPF
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCH
EWVSASTHRKKNNWEQASLTAIHRESPQRVRPGCPHFGLHAGACGGCKMQHLHVGAQVAV
HHHCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHH
KQRVLEDNLWHLGKVKAETVLRPIEGPAWGYRYRARLAVRHVEKKGKVLVGFHERKSRYI
HHHHHHHCCHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHCCCEE
ADMEVCPVLPPHVSAMLLPLRALIASMDARDTCPQIELACGDDVTALVLRHLEPLSEADL
ECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCCHHHH
GRLRSFAAAHGVQWWLQPKGPDTVHLLDEGGPQLNYALPDFGITMPFKPTDFTQVNPHIN
HHHHHHHHHCCCEEEECCCCCCEEEEECCCCCEEEEECCCCCEECCCCCCCCCCCCHHHH
RVLVTRALRLLDAGRTDRVIDWFCGLGNFTLPIATQAREVVGIEGSEALVARSRENYRKN
HHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEECCCCHHHCCCCCCHHHHHHHHHHHHCC
QDDRPQGQALAPATFVARNLFEMTPEMLIADGVADKWLVDPPREGAFALAKALADIHQAR
CCCCCCCCCCCHHHHHHHHHHHHCHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IGAEDAPALPASAEGWTPPQRIVYVSCNPATLARDAGLLVHQAGYRCVAAGVVNMFPHTA
CCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCEEECCCCHHHHHHHHHHCCCHH
HVESMAVFERV
HHHHHHHHHCC
>Mature Secondary Structure 
SDPTEHPEILQDPSSSAPVQGRTDLPPGWLEVTSMDMDAQGVARRPDGKVVFIDGALPF
CCCCCCCHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCH
EWVSASTHRKKNNWEQASLTAIHRESPQRVRPGCPHFGLHAGACGGCKMQHLHVGAQVAV
HHHCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHH
KQRVLEDNLWHLGKVKAETVLRPIEGPAWGYRYRARLAVRHVEKKGKVLVGFHERKSRYI
HHHHHHHCCHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHCCCEE
ADMEVCPVLPPHVSAMLLPLRALIASMDARDTCPQIELACGDDVTALVLRHLEPLSEADL
ECCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCCHHHH
GRLRSFAAAHGVQWWLQPKGPDTVHLLDEGGPQLNYALPDFGITMPFKPTDFTQVNPHIN
HHHHHHHHHCCCEEEECCCCCCEEEEECCCCCEEEEECCCCCEECCCCCCCCCCCCHHHH
RVLVTRALRLLDAGRTDRVIDWFCGLGNFTLPIATQAREVVGIEGSEALVARSRENYRKN
HHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEECCCCHHHCCCCCCHHHHHHHHHHHHCC
QDDRPQGQALAPATFVARNLFEMTPEMLIADGVADKWLVDPPREGAFALAKALADIHQAR
CCCCCCCCCCCHHHHHHHHHHHHCHHHEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
IGAEDAPALPASAEGWTPPQRIVYVSCNPATLARDAGLLVHQAGYRCVAAGVVNMFPHTA
CCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHCCCCEEECCCCHHHHHHHHHHCCCHH
HVESMAVFERV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA