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The map label for this gene is mltB [H]

Identifier: 120611895

GI number: 120611895

Start: 3568660

End: 3570072

Strand: Direct

Name: mltB [H]

Synonym: Aave_3237

Alternate gene names: 120611895

Gene position: 3568660-3570072 (Clockwise)

Preceding gene: 120611891

Following gene: 120611899

Centisome position: 66.67

GC content: 74.66

Gene sequence:

>1413_bases
ATGCAACGAAGAAACACGACGACCGCCTCCGCCCTCCGTCCCTCCTTCCGCTTCCACGCCGAGCCCGCCAGGCCGTGGTG
GCCCGCCGCCGGGGCAGGCCTGCTCTGCGCCCTGCTGGCCGGGTGCGCGGCCCCTCCTCCCGCGGCGGCCGTGCCCGCCG
CCCCGCCTGCACCGGCCGCCACACCGGCGGTGCCCCCGGCGCCCCCCCCGGACACGCCGGCCACCATGGCCCCAGCCGCC
CAGCCCGCCACGCCGGCGGCCGATGCCGCCCTGCCTTCCGCAGAGGCCGAACAGGCCGGCTTCGCGGAGTGGATCGCGGG
CTTTCGCCAGGAAGCCCTGCAGGCCGGCATCCGCCCCGAGGCGGTCGCCGCCGCCCTGGACGGCGCGCAGTGGCTGCCCC
AGGTGATCGAACTCGACCGCTCACAGCCCGAATTCACGCGCACGCCCTGGGCCTACCTGGACAGCGCCGTCTCGCCCACG
CGCATCCAGCGCGGCCGCGACGCGCTGGCCGAGCAGCGCGCCGCCCTGGACTCGGCGGCCGCGCGCTATGGCGTGCCCGC
CGAGGTCATCACCGCCATCTGGGGCATGGAAAGCAACTACGGATCGAATTTCGGCACCTTCCGCACCGTCGATGCGCTCG
CCACGCTGGCCTACGAGGGCCGCCGCCGCGACTGGGCGCGCGGCGAACTGCTGGCCGCGCTGCGCATCATCGACCAGGGC
GACATCGCCGCCGACCGCATGATCGGCTCCTGGGCCGGCGCCATGGGCCACACCCAGTTCCTGCCGTCCGTGTTCCTGGG
CTATGCAGTGGACGCCGATGGCGACGGCCACCGCGACATCTGGGGCAGCGTCCCGGACGTCGCCGCATCCACGGCGAACT
TCCTGTCGCGCATGGGCTGGCAGCCGGGCCAGCCCTGGGGCGTGGAAGTGCGCCTGCCGCCGGGCTTCGACTACGAGCGC
GCGGAACTGGCCGTGCGCCACCCGTCCACCCAGTGGGCGGCCGAAGGCGTGCAGGCCATCGATGGCCAGCCCCTGCCCGC
GCTGGACGGCGCATCCATCCTCGTGCCCGCGGGCGCGCGCGGCCCGGCCTTCATGGTGGGGCCGAATTTCCGGGTGATCC
TGCGCTACAACAACTCCATCAACTACGCCCTGGGCGTGGGCCTGCTCGCGAGCCAGATCGCCGGCGGCCCTGGCGTCACG
GCCCCGTGGCCCCGCGACCTGCAGCCGCTGACCCGCACCCAGATTCGCGACCTGCAGAATGCCCTCAACGCACGCGGCTT
CGCAGCGGGCACGGCGGACGGGGTGATGGGCCCCGCCACGCGCGCCGGCCTGCGCCGCTTCCAGCAGAGCATCGGGCAGG
TGGCGGACGGGTATCCCACGCTCGAGCTGCTGCAGCGACTGCAGGCGCCCTGA

Upstream 100 bases:

>100_bases
GTGCCAGAAAAACGTGTGCATGGATCGGTGAAGCGCAGCGGCGCGTAAAACTCCAGCCCGCAAGTATGCCGGCACCGCCG
CGGCGCGTGGACAATGGGGC

Downstream 100 bases:

>100_bases
CAGCCTGCCGGCGTGCCCCCTCCCGCGGCCGTCCGCCGGGGCCTGCGCAAAGAAAAAAGCCCGCGGACCCTGGCGGGGCC
GCGGGCTCGGCGCTGTGCCG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MQRRNTTTASALRPSFRFHAEPARPWWPAAGAGLLCALLAGCAAPPPAAAVPAAPPAPAATPAVPPAPPPDTPATMAPAA
QPATPAADAALPSAEAEQAGFAEWIAGFRQEALQAGIRPEAVAAALDGAQWLPQVIELDRSQPEFTRTPWAYLDSAVSPT
RIQRGRDALAEQRAALDSAAARYGVPAEVITAIWGMESNYGSNFGTFRTVDALATLAYEGRRRDWARGELLAALRIIDQG
DIAADRMIGSWAGAMGHTQFLPSVFLGYAVDADGDGHRDIWGSVPDVAASTANFLSRMGWQPGQPWGVEVRLPPGFDYER
AELAVRHPSTQWAAEGVQAIDGQPLPALDGASILVPAGARGPAFMVGPNFRVILRYNNSINYALGVGLLASQIAGGPGVT
APWPRDLQPLTRTQIRDLQNALNARGFAAGTADGVMGPATRAGLRRFQQSIGQVADGYPTLELLQRLQAP

Sequences:

>Translated_470_residues
MQRRNTTTASALRPSFRFHAEPARPWWPAAGAGLLCALLAGCAAPPPAAAVPAAPPAPAATPAVPPAPPPDTPATMAPAA
QPATPAADAALPSAEAEQAGFAEWIAGFRQEALQAGIRPEAVAAALDGAQWLPQVIELDRSQPEFTRTPWAYLDSAVSPT
RIQRGRDALAEQRAALDSAAARYGVPAEVITAIWGMESNYGSNFGTFRTVDALATLAYEGRRRDWARGELLAALRIIDQG
DIAADRMIGSWAGAMGHTQFLPSVFLGYAVDADGDGHRDIWGSVPDVAASTANFLSRMGWQPGQPWGVEVRLPPGFDYER
AELAVRHPSTQWAAEGVQAIDGQPLPALDGASILVPAGARGPAFMVGPNFRVILRYNNSINYALGVGLLASQIAGGPGVT
APWPRDLQPLTRTQIRDLQNALNARGFAAGTADGVMGPATRAGLRRFQQSIGQVADGYPTLELLQRLQAP
>Mature_470_residues
MQRRNTTTASALRPSFRFHAEPARPWWPAAGAGLLCALLAGCAAPPPAAAVPAAPPAPAATPAVPPAPPPDTPATMAPAA
QPATPAADAALPSAEAEQAGFAEWIAGFRQEALQAGIRPEAVAAALDGAQWLPQVIELDRSQPEFTRTPWAYLDSAVSPT
RIQRGRDALAEQRAALDSAAARYGVPAEVITAIWGMESNYGSNFGTFRTVDALATLAYEGRRRDWARGELLAALRIIDQG
DIAADRMIGSWAGAMGHTQFLPSVFLGYAVDADGDGHRDIWGSVPDVAASTANFLSRMGWQPGQPWGVEVRLPPGFDYER
AELAVRHPSTQWAAEGVQAIDGQPLPALDGASILVPAGARGPAFMVGPNFRVILRYNNSINYALGVGLLASQIAGGPGVT
APWPRDLQPLTRTQIRDLQNALNARGFAAGTADGVMGPATRAGLRRFQQSIGQVADGYPTLELLQRLQAP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=297, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 49510; Mature: 49510

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRNTTTASALRPSFRFHAEPARPWWPAAGAGLLCALLAGCAAPPPAAAVPAAPPAPAA
CCCCCCCHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
TPAVPPAPPPDTPATMAPAAQPATPAADAALPSAEAEQAGFAEWIAGFRQEALQAGIRPE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
AVAAALDGAQWLPQVIELDRSQPEFTRTPWAYLDSAVSPTRIQRGRDALAEQRAALDSAA
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ARYGVPAEVITAIWGMESNYGSNFGTFRTVDALATLAYEGRRRDWARGELLAALRIIDQG
HHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
DIAADRMIGSWAGAMGHTQFLPSVFLGYAVDADGDGHRDIWGSVPDVAASTANFLSRMGW
CHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
QPGQPWGVEVRLPPGFDYERAELAVRHPSTQWAAEGVQAIDGQPLPALDGASILVPAGAR
CCCCCCCEEEECCCCCCHHHHHEEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCC
GPAFMVGPNFRVILRYNNSINYALGVGLLASQIAGGPGVTAPWPRDLQPLTRTQIRDLQN
CCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
ALNARGFAAGTADGVMGPATRAGLRRFQQSIGQVADGYPTLELLQRLQAP
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
>Mature Secondary Structure
MQRRNTTTASALRPSFRFHAEPARPWWPAAGAGLLCALLAGCAAPPPAAAVPAAPPAPAA
CCCCCCCHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
TPAVPPAPPPDTPATMAPAAQPATPAADAALPSAEAEQAGFAEWIAGFRQEALQAGIRPE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
AVAAALDGAQWLPQVIELDRSQPEFTRTPWAYLDSAVSPTRIQRGRDALAEQRAALDSAA
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
ARYGVPAEVITAIWGMESNYGSNFGTFRTVDALATLAYEGRRRDWARGELLAALRIIDQG
HHCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCC
DIAADRMIGSWAGAMGHTQFLPSVFLGYAVDADGDGHRDIWGSVPDVAASTANFLSRMGW
CHHHHHHHHHHHHHCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCC
QPGQPWGVEVRLPPGFDYERAELAVRHPSTQWAAEGVQAIDGQPLPALDGASILVPAGAR
CCCCCCCEEEECCCCCCHHHHHEEEECCCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCC
GPAFMVGPNFRVILRYNNSINYALGVGLLASQIAGGPGVTAPWPRDLQPLTRTQIRDLQN
CCEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
ALNARGFAAGTADGVMGPATRAGLRRFQQSIGQVADGYPTLELLQRLQAP
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]