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The map label for this gene is bfpB [H]

Identifier: 120611389

GI number: 120611389

Start: 2991675

End: 2993375

Strand: Reverse

Name: bfpB [H]

Synonym: Aave_2725

Alternate gene names: 120611389

Gene position: 2993375-2991675 (Counterclockwise)

Preceding gene: 120611390

Following gene: 120611388

Centisome position: 55.92

GC content: 66.49

Gene sequence:

>1701_bases
ATGAAGAATGAACGACGTGGTCGACGCCTCGTCTGCGGGCTCGTGGCCACGACCTTGGTACTGCAGGCCTGCTCCACCAT
TCAGGAGACGGAAGCGAACGCGCAGAAAGCGCGGGAGCAGACACGCCGCATTTTGGACGAGCGTACCGCCCAGGGAGCAC
CCGCCGGCGTGACCCGGCTGGCGGGCCCGCGCATCGCCGGGCGGGAAATCAAGCTCGTCAAGAAGGAGGCGCTGCCCGAC
ATCTTCGACGCAGAGATCGGCTATGCGAGCCATGGAACGCAGCGCCTGAGCGAAGTGCTCGAAGAACTCGGTGCACGAAC
CGGGATGGCGATCTCGTCCACTGAGATCGCGAGCATGCCGCGTACCGGACAGGCCGCGCAGGCGAGCGCCGGCAGCGAGC
AGCCCATCACCGCTTACGTTCAGGTCGAGTTCAGCGGGAAGGTCCGCAGCCTTTTCGACGACCTGGCACAGCGCGCCGGC
GCCAGCTGGCGGTACGTGAAGTCGACGAACTCGGTGCAGTTCTACCGATTCGAGACACGCACATACCCGATGCACCTCCC
ATCCGGAGCCAAGTCGCTGCAGGCGGCGATCAGCCTGTCGTCCACGAGCTTGAGCAGCGGTGGCGGCAGCGGCTCGGCCG
GAGGCGCGACAGGCGCGGGCGGCTCGTCTGGAGGAGCCGGAGGCGGCGGAAACGTATCGGTGACGCAGAGCCAGGTCATC
GACCCATGGACGTCGATCATGGCGTCGGTGCAGGCCATCCTGGGTGCGAGCCAGGACACGACGGCCACCGGCCGCGCTGT
GCCGGCGCTGGCCGCGGGCGCCAGCGGTGCAGCGCCTGCCGCAGCGAGCAAGCTCTCCGCATCCGGACTGGATGGCTTCG
CGACGGCCGCGCCTGAGCTCGGCATCGTCACCGTCACGGCAAGGCCACTCAGCCAGGCCCGCATTGCAGCCCTCCTCCAC
TCGATCAACCAGCGTTTCGCGCGCAACCTCCTCATCGACGTGACGATCTACAACGTCTCGATGACCAAGGAGGAGACTGC
CGGCGTGTCCATGGACCTGGTGTACCGGCGCCTCAACGGCAATGGAGTGGGCGTTGCCGGCGCGGCGCCGCTGCAGCCAG
CGAACGGCCAGCCCGGGCGCATCACCCTGAGCTTCTCCGACCCCGCTTCGCGTCTGGCCGGCTCGCGCATGGTTGCCGAA
GCACTCCAATCCGTAGGCAACGTGGCGCTCTACAAGAAGGGCCAGTTCATGGCCATCAACGGCCAGCCCAGCCCGTTCCA
GGTGGTTGATGACGAGGAGTACAACACATCGGTGTCGGCCACTCAGACCGCCAATGTAGGCACACAACTCGCCACGCAGA
AGGCCGTGCTCACCACGGGCCTTACCGGCAACGTGCTGCCGCTCATCCTGGGGGACAACCGGATCCTGTTGCAGTACCAG
ATGGAGATCAGCGCGGTCAGCAACATGAACCCGGCGGTGGTCAACGGCATCCTGACCTACTCCCCCAAGCGCTCGCGCCA
GAGCTTCCAGCAGCAGGCGTTTCTGAAGGATGGCGATGCCATCGTGCTCTTCGGCTACGACAGCGGCGGCGACGAGGCCG
CGGGCCAGGTGTCGCTCTCGGGCGGCAGCAAGCGGGGCAGCGGCGACCGGACCATGTCCGTGATCGTCATGCAAGTCTTC
GGGGGGCAGAAAAATGGCTGA

Upstream 100 bases:

>100_bases
ATCTTGCGCTCGGAGGGCAAGCAGATCGCCCTGTCGGTCGCTGCCGCCAACCGCCTCATGGAGGTCACGTCCATGGATAT
CACCGATCGGAGCGAGAGCG

Downstream 100 bases:

>100_bases
CGGAAAGTCCATCGGCTGGGGAATGCTGGCGGCCGTCGCCTTGGCAGCAGGCGCCCACGCGCAGCCCAGGACGCTCGCCG
ATCGCGCGGCCGAGGCCGAC

Product: Type II secretory pathway component PulD-like protein

Products: NA

Alternate protein names: Bundle-forming pilus B [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKNERRGRRLVCGLVATTLVLQACSTIQETEANAQKAREQTRRILDERTAQGAPAGVTRLAGPRIAGREIKLVKKEALPD
IFDAEIGYASHGTQRLSEVLEELGARTGMAISSTEIASMPRTGQAAQASAGSEQPITAYVQVEFSGKVRSLFDDLAQRAG
ASWRYVKSTNSVQFYRFETRTYPMHLPSGAKSLQAAISLSSTSLSSGGGSGSAGGATGAGGSSGGAGGGGNVSVTQSQVI
DPWTSIMASVQAILGASQDTTATGRAVPALAAGASGAAPAAASKLSASGLDGFATAAPELGIVTVTARPLSQARIAALLH
SINQRFARNLLIDVTIYNVSMTKEETAGVSMDLVYRRLNGNGVGVAGAAPLQPANGQPGRITLSFSDPASRLAGSRMVAE
ALQSVGNVALYKKGQFMAINGQPSPFQVVDDEEYNTSVSATQTANVGTQLATQKAVLTTGLTGNVLPLILGDNRILLQYQ
MEISAVSNMNPAVVNGILTYSPKRSRQSFQQQAFLKDGDAIVLFGYDSGGDEAAGQVSLSGGSKRGSGDRTMSVIVMQVF
GGQKNG

Sequences:

>Translated_566_residues
MKNERRGRRLVCGLVATTLVLQACSTIQETEANAQKAREQTRRILDERTAQGAPAGVTRLAGPRIAGREIKLVKKEALPD
IFDAEIGYASHGTQRLSEVLEELGARTGMAISSTEIASMPRTGQAAQASAGSEQPITAYVQVEFSGKVRSLFDDLAQRAG
ASWRYVKSTNSVQFYRFETRTYPMHLPSGAKSLQAAISLSSTSLSSGGGSGSAGGATGAGGSSGGAGGGGNVSVTQSQVI
DPWTSIMASVQAILGASQDTTATGRAVPALAAGASGAAPAAASKLSASGLDGFATAAPELGIVTVTARPLSQARIAALLH
SINQRFARNLLIDVTIYNVSMTKEETAGVSMDLVYRRLNGNGVGVAGAAPLQPANGQPGRITLSFSDPASRLAGSRMVAE
ALQSVGNVALYKKGQFMAINGQPSPFQVVDDEEYNTSVSATQTANVGTQLATQKAVLTTGLTGNVLPLILGDNRILLQYQ
MEISAVSNMNPAVVNGILTYSPKRSRQSFQQQAFLKDGDAIVLFGYDSGGDEAAGQVSLSGGSKRGSGDRTMSVIVMQVF
GGQKNG
>Mature_566_residues
MKNERRGRRLVCGLVATTLVLQACSTIQETEANAQKAREQTRRILDERTAQGAPAGVTRLAGPRIAGREIKLVKKEALPD
IFDAEIGYASHGTQRLSEVLEELGARTGMAISSTEIASMPRTGQAAQASAGSEQPITAYVQVEFSGKVRSLFDDLAQRAG
ASWRYVKSTNSVQFYRFETRTYPMHLPSGAKSLQAAISLSSTSLSSGGGSGSAGGATGAGGSSGGAGGGGNVSVTQSQVI
DPWTSIMASVQAILGASQDTTATGRAVPALAAGASGAAPAAASKLSASGLDGFATAAPELGIVTVTARPLSQARIAALLH
SINQRFARNLLIDVTIYNVSMTKEETAGVSMDLVYRRLNGNGVGVAGAAPLQPANGQPGRITLSFSDPASRLAGSRMVAE
ALQSVGNVALYKKGQFMAINGQPSPFQVVDDEEYNTSVSATQTANVGTQLATQKAVLTTGLTGNVLPLILGDNRILLQYQ
MEISAVSNMNPAVVNGILTYSPKRSRQSFQQQAFLKDGDAIVLFGYDSGGDEAAGQVSLSGGSKRGSGDRTMSVIVMQVF
GGQKNG

Specific function: Is absolutely required for pilus biogenesis, and for EPEC localized adherence and autoaggregation. Acts at a step in the BFP biogenic pathway after production and processing of the structural pilus subunit BfpA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011514
- InterPro:   IPR004846 [H]

Pfam domain/function: PF00263 Secretin; PF07655 Secretin_N_2 [H]

EC number: NA

Molecular weight: Translated: 58599; Mature: 58599

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNERRGRRLVCGLVATTLVLQACSTIQETEANAQKAREQTRRILDERTAQGAPAGVTRL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AGPRIAGREIKLVKKEALPDIFDAEIGYASHGTQRLSEVLEELGARTGMAISSTEIASMP
CCCCCCCCCEEEEHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEECCHHHHCCC
RTGQAAQASAGSEQPITAYVQVEFSGKVRSLFDDLAQRAGASWRYVKSTNSVQFYRFETR
CCCCCHHCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECC
TYPMHLPSGAKSLQAAISLSSTSLSSGGGSGSAGGATGAGGSSGGAGGGGNVSVTQSQVI
EEEEECCCCHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHC
DPWTSIMASVQAILGASQDTTATGRAVPALAAGASGAAPAAASKLSASGLDGFATAAPEL
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHCCCCCCCHHHCCCCC
GIVTVTARPLSQARIAALLHSINQRFARNLLIDVTIYNVSMTKEETAGVSMDLVYRRLNG
CEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCHHHCCCCHHHEEEEECC
NGVGVAGAAPLQPANGQPGRITLSFSDPASRLAGSRMVAEALQSVGNVALYKKGQFMAIN
CCEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEC
GQPSPFQVVDDEEYNTSVSATQTANVGTQLATQKAVLTTGLTGNVLPLILGDNRILLQYQ
CCCCCCEEECCCCCCCCCCCEECCCCCHHHHHHHHHEEECCCCCEEEEEECCCEEEEEEE
MEISAVSNMNPAVVNGILTYSPKRSRQSFQQQAFLKDGDAIVLFGYDSGGDEAAGQVSLS
EEEHHHCCCCHHHHHEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEC
GGSKRGSGDRTMSVIVMQVFGGQKNG
CCCCCCCCCHHHHHEEEEHHCCCCCC
>Mature Secondary Structure
MKNERRGRRLVCGLVATTLVLQACSTIQETEANAQKAREQTRRILDERTAQGAPAGVTRL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AGPRIAGREIKLVKKEALPDIFDAEIGYASHGTQRLSEVLEELGARTGMAISSTEIASMP
CCCCCCCCCEEEEHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCCCEECCHHHHCCC
RTGQAAQASAGSEQPITAYVQVEFSGKVRSLFDDLAQRAGASWRYVKSTNSVQFYRFETR
CCCCCHHCCCCCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECC
TYPMHLPSGAKSLQAAISLSSTSLSSGGGSGSAGGATGAGGSSGGAGGGGNVSVTQSQVI
EEEEECCCCHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHC
DPWTSIMASVQAILGASQDTTATGRAVPALAAGASGAAPAAASKLSASGLDGFATAAPEL
CHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHCCCCCCCHHHCCCCC
GIVTVTARPLSQARIAALLHSINQRFARNLLIDVTIYNVSMTKEETAGVSMDLVYRRLNG
CEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEEECCHHHCCCCHHHEEEEECC
NGVGVAGAAPLQPANGQPGRITLSFSDPASRLAGSRMVAEALQSVGNVALYKKGQFMAIN
CCEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEC
GQPSPFQVVDDEEYNTSVSATQTANVGTQLATQKAVLTTGLTGNVLPLILGDNRILLQYQ
CCCCCCEEECCCCCCCCCCCEECCCCCHHHHHHHHHEEECCCCCEEEEEECCCEEEEEEE
MEISAVSNMNPAVVNGILTYSPKRSRQSFQQQAFLKDGDAIVLFGYDSGGDEAAGQVSLS
EEEHHHCCCCHHHHHEEEECCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEC
GGSKRGSGDRTMSVIVMQVFGGQKNG
CCCCCCCCCHHHHHEEEEHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8626330; 10496929; 8932312 [H]