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The map label for this gene is tcpT [H]

Identifier: 120611386

GI number: 120611386

Start: 2987862

End: 2989370

Strand: Reverse

Name: tcpT [H]

Synonym: Aave_2722

Alternate gene names: 120611386

Gene position: 2989370-2987862 (Counterclockwise)

Preceding gene: 120611387

Following gene: 120611385

Centisome position: 55.85

GC content: 64.15

Gene sequence:

>1509_bases
ATGGCTTCGGGCCAGTACCTGGACAAGCCCAAGACGGCCGGCTCGCGCAGGTTCAGCACCTATCTGCTGATGCCCCCGGC
GGCGTTCCTGTCAGCGAGGGCGATGATGGACGTGGCACAGCGGCTGGAAGAGGACGGGCATACAAACATTCTTCCGCTGC
TCACCACCACGGAACTGATCCAGGCCGTTCACCACCGCGCCAACCGTGGTGTTGACAGCGGCAACAGCATCCAGACAACC
ACACAGGACGCAGCACGCGAACTCCTGCTGGAGGCCGACAGCCTCCAGTCCTCGGACATCCACATCGAGACGCGCGGAGA
CGTCGCCGTCGTCCAGTTTCGGGTTCACGGACGTCGCCAGCGGAGGGCCGACATCTCCCGAGTGACGGCCGAGGCGATCG
CGCATCTGCTCTTCAATTTCGAGTCGGCCGACGGGGCCCGCAAAGGCAGCTGGAACCCTAAGGAAGTGAAGGACACGTCG
TTCGACGTATGGGAGGATCCCGCCGAGCGCACGAAGCGCCTCATGCGCGTGCGCTTCCACTCCACGCCAATCCATCCAGA
GGGCAACTTCCAGATCGTCATGCGGCTCTTGCGGCCCGCGGCCAGGACGGGCGGCGCGCGGCCCCTGGCGCAGATCGGCT
ACACCCAGGACCAGCTGGCGCAGATCGAGGAAATGCTGGTCGGTGGATCTGGCCTGGTGCTGATGGTCGGGCCTACCAAT
TCCGGCAAGTCGTCGTCGCTGCACAGCTTCGTCGAGCATCTCTTCCAGACCCGCGGCAGGCACATCAAGGTGACGACCAT
CGAGAACCCGGTCGAGAACGAACTCCTGGGTGCGTGCCAGATCTCGGCGAGCAAGGACAACTTCCAGGCGTACCTGGAGG
CCTCGCTGCGGCAGGATCCCGACATCGTCATGGTGGGCGAGATCCGCGAGAAGCTGGCGGCGCTGACGGTGAAGGACCTG
GTGCTGGCCGGGCACAAGATCCCGGCCACGCTCCACGCATACTCGGCCACCGCAGCGTTCTCCCGGCTCATGCAGCTCGG
CGTGGAAAAGGACCTGTTGACCATGCCCGGTTTCATCTCCGGCGTGGTGTATCAGCGCCTGATCCCCACTGTGTGCCCCC
ACTGTGGCCACGACTTCAAGTCCGCGCATCAGAACGGCCTGGTGTCGAAGTCCCTCTTCGATCGCCTGACGTCGGTCTGC
GACCTGCAGGAGCACCACGTCCGCTTCGTCAACAGCGAAGGCTGCGTCGAATGCGGCCACTCTGGAATCGTCGATCGAAC
ACCGGCCGCAGAGGTGTTGATCCCCGATTCCACTTTCCTGAACCACATCAAGAACGACGACCTCGGCGCTGCCCGCGACC
ACTGGCTTCGCATACTCGGCAAGCCGGCAGGCTACGGACTGGGCGCTACCGCTCTCGCCCATGCGATCCTCAAGATGCGC
CAGGGGCAGGTAGATCCCCGTGACGTCGAAACCGAACTCGGCGTTCTGCGAGACGAGAGTACCCGCTGA

Upstream 100 bases:

>100_bases
CGACTACATCAACCTGTTCGCGCTCAAGCGGCCCGTCTCGGAGATCCACAGAGCGACCTTTGCCGCAATCCGTAGCGAGT
TGTTGGTCGACCAGAAGCTC

Downstream 100 bases:

>100_bases
CCATGATGCGATTCAAGGCCCTCTACAGACTGCGGCTGACGTCGGCCGTGCGTGCGGACTTCTATTCCGTTCTCGCGTCG
CTGACCGGGACAGCCGGGCG

Product: type II secretion system protein E

Products: NA

Alternate protein names: TCP pilus biosynthesis protein tcpT [H]

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MASGQYLDKPKTAGSRRFSTYLLMPPAAFLSARAMMDVAQRLEEDGHTNILPLLTTTELIQAVHHRANRGVDSGNSIQTT
TQDAARELLLEADSLQSSDIHIETRGDVAVVQFRVHGRRQRRADISRVTAEAIAHLLFNFESADGARKGSWNPKEVKDTS
FDVWEDPAERTKRLMRVRFHSTPIHPEGNFQIVMRLLRPAARTGGARPLAQIGYTQDQLAQIEEMLVGGSGLVLMVGPTN
SGKSSSLHSFVEHLFQTRGRHIKVTTIENPVENELLGACQISASKDNFQAYLEASLRQDPDIVMVGEIREKLAALTVKDL
VLAGHKIPATLHAYSATAAFSRLMQLGVEKDLLTMPGFISGVVYQRLIPTVCPHCGHDFKSAHQNGLVSKSLFDRLTSVC
DLQEHHVRFVNSEGCVECGHSGIVDRTPAAEVLIPDSTFLNHIKNDDLGAARDHWLRILGKPAGYGLGATALAHAILKMR
QGQVDPRDVETELGVLRDESTR

Sequences:

>Translated_502_residues
MASGQYLDKPKTAGSRRFSTYLLMPPAAFLSARAMMDVAQRLEEDGHTNILPLLTTTELIQAVHHRANRGVDSGNSIQTT
TQDAARELLLEADSLQSSDIHIETRGDVAVVQFRVHGRRQRRADISRVTAEAIAHLLFNFESADGARKGSWNPKEVKDTS
FDVWEDPAERTKRLMRVRFHSTPIHPEGNFQIVMRLLRPAARTGGARPLAQIGYTQDQLAQIEEMLVGGSGLVLMVGPTN
SGKSSSLHSFVEHLFQTRGRHIKVTTIENPVENELLGACQISASKDNFQAYLEASLRQDPDIVMVGEIREKLAALTVKDL
VLAGHKIPATLHAYSATAAFSRLMQLGVEKDLLTMPGFISGVVYQRLIPTVCPHCGHDFKSAHQNGLVSKSLFDRLTSVC
DLQEHHVRFVNSEGCVECGHSGIVDRTPAAEVLIPDSTFLNHIKNDDLGAARDHWLRILGKPAGYGLGATALAHAILKMR
QGQVDPRDVETELGVLRDESTR
>Mature_501_residues
ASGQYLDKPKTAGSRRFSTYLLMPPAAFLSARAMMDVAQRLEEDGHTNILPLLTTTELIQAVHHRANRGVDSGNSIQTTT
QDAARELLLEADSLQSSDIHIETRGDVAVVQFRVHGRRQRRADISRVTAEAIAHLLFNFESADGARKGSWNPKEVKDTSF
DVWEDPAERTKRLMRVRFHSTPIHPEGNFQIVMRLLRPAARTGGARPLAQIGYTQDQLAQIEEMLVGGSGLVLMVGPTNS
GKSSSLHSFVEHLFQTRGRHIKVTTIENPVENELLGACQISASKDNFQAYLEASLRQDPDIVMVGEIREKLAALTVKDLV
LAGHKIPATLHAYSATAAFSRLMQLGVEKDLLTMPGFISGVVYQRLIPTVCPHCGHDFKSAHQNGLVSKSLFDRLTSVCD
LQEHHVRFVNSEGCVECGHSGIVDRTPAAEVLIPDSTFLNHIKNDDLGAARDHWLRILGKPAGYGLGATALAHAILKMRQ
GQVDPRDVETELGVLRDESTR

Specific function: Involved in the translocation of the tcpA pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=351, Percent_Identity=30.7692307692308, Blast_Score=118, Evalue=9e-28,
Organism=Escherichia coli, GI1786296, Length=342, Percent_Identity=29.8245614035088, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI87082188, Length=159, Percent_Identity=34.5911949685535, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55284; Mature: 55152

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASGQYLDKPKTAGSRRFSTYLLMPPAAFLSARAMMDVAQRLEEDGHTNILPLLTTTELI
CCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
QAVHHRANRGVDSGNSIQTTTQDAARELLLEADSLQSSDIHIETRGDVAVVQFRVHGRRQ
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCHH
RRADISRVTAEAIAHLLFNFESADGARKGSWNPKEVKDTSFDVWEDPAERTKRLMRVRFH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
STPIHPEGNFQIVMRLLRPAARTGGARPLAQIGYTQDQLAQIEEMLVGGSGLVLMVGPTN
CCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCC
SGKSSSLHSFVEHLFQTRGRHIKVTTIENPVENELLGACQISASKDNFQAYLEASLRQDP
CCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHEECCCCHHHHHHHHHHHCCCC
DIVMVGEIREKLAALTVKDLVLAGHKIPATLHAYSATAAFSRLMQLGVEKDLLTMPGFIS
CEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHH
GVVYQRLIPTVCPHCGHDFKSAHQNGLVSKSLFDRLTSVCDLQEHHVRFVNSEGCVECGH
HHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHEEECCCCCHHCCC
SGIVDRTPAAEVLIPDSTFLNHIKNDDLGAARDHWLRILGKPAGYGLGATALAHAILKMR
CCCCCCCCCCCEEECCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
QGQVDPRDVETELGVLRDESTR
CCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ASGQYLDKPKTAGSRRFSTYLLMPPAAFLSARAMMDVAQRLEEDGHTNILPLLTTTELI
CCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHH
QAVHHRANRGVDSGNSIQTTTQDAARELLLEADSLQSSDIHIETRGDVAVVQFRVHGRRQ
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEEEECCCHH
RRADISRVTAEAIAHLLFNFESADGARKGSWNPKEVKDTSFDVWEDPAERTKRLMRVRFH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHC
STPIHPEGNFQIVMRLLRPAARTGGARPLAQIGYTQDQLAQIEEMLVGGSGLVLMVGPTN
CCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCC
SGKSSSLHSFVEHLFQTRGRHIKVTTIENPVENELLGACQISASKDNFQAYLEASLRQDP
CCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHEECCCCHHHHHHHHHHHCCCC
DIVMVGEIREKLAALTVKDLVLAGHKIPATLHAYSATAAFSRLMQLGVEKDLLTMPGFIS
CEEEHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHH
GVVYQRLIPTVCPHCGHDFKSAHQNGLVSKSLFDRLTSVCDLQEHHVRFVNSEGCVECGH
HHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHEEECCCCCHHCCC
SGIVDRTPAAEVLIPDSTFLNHIKNDDLGAARDHWLRILGKPAGYGLGATALAHAILKMR
CCCCCCCCCCCEEECCHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
QGQVDPRDVETELGVLRDESTR
CCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8097178; 10952301 [H]