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The map label for this gene is pilT [H]

Identifier: 120611380

GI number: 120611380

Start: 2982771

End: 2984003

Strand: Reverse

Name: pilT [H]

Synonym: Aave_2716

Alternate gene names: 120611380

Gene position: 2984003-2982771 (Counterclockwise)

Preceding gene: 120611381

Following gene: 120611375

Centisome position: 55.75

GC content: 64.31

Gene sequence:

>1233_bases
ATGAAAAGCATCATCGACAACGACACGTTCGGCCGGTTCGTGGTCCAGACCTTCCATCAGGGGGAAGGCAAGCTCTACAC
CGATGCCTACATCCAGCCGGGTCAGCCGGTCCGTGTTCGGTTGGGAAGCCATCGCTGGGCCGACCTCGAGTACATCGTGG
GTGACCAGAAGGAAAAGCGGAACCTGGTCTTCAGTGCCGAGGACTTCGACGCGCTCATGGCCAACCTGTTCCAGAAGCTC
ACGCACCGCGCCGGCGAGCCGGCAAGAAAGTGGCAGGACCTGGTCGCGCAATCGAACGGCTGCCTTCACCCCATGATCAC
CCTCTCGGCGCCGTCTCCCACAGGCGACACGACCTTTCGCGTACGGTTCACCCTGCAACGCCAGGACATGGGCGGCTACG
GGTTGATGCTGCGGTGTCTGCGGCCCGTGCCGGAATCGATCGAGGAGCTCGGGCTTCCCAGCACCGTGCATGCGCTCTGC
CAGGCCGGGCACGGCCTGGTGCTCGTCACCGGCCGCACTGGCGCCGGCAAGAGCACCACCACGGCCGCGCTTATCCACCA
GATCAACCGGAGCACGACGTCGAACATCGTGGTGATCGAGGAGCCGATCGAGTTCATCCATCGCCCGGACAAGGCCCGGT
TCACCTATCGAGAGGTCGGCGTCGACGTCGACACGTACGCCAATGGTGTGGAGCAGGTGTTGCGATACGTCCCCGACGTC
ATCAGCATCGGAGAGATCCGGGACCCCGAGACGATGCGCGCCGCGGTGCGGGCGGCCGAATCGGGCCACCTGGTCTTCGG
CACCATCCACGCCGCGAACACCACAGGCGCCATCCGCAAGGCACTGGGCTATCTCGATTCGCCTGGCGAAAAACTCTCCT
TCGCGACCAACCTGGTCGGTGTGATCGCGCAGGCGCTCCTTCCGGCCAAGACCGGTACCGGCAAGGCGCTGGCATTCGAG
GTGATGGATTTCACGACAAAGCGCAGCGGCACGACGCCGCTGGCCGAGACGGTGGAGAGGATGCTCTCGGCCAAGAGCGA
CGGCACCGAGCTGCGTCAGTTCGAGACGGACTTTCTGAGCTCGTCCAACCTCGGCATGGGCAACAGCCCGTTCATCCAGA
GCGTGGTTACTCTCGTGCGCAAAGGGCACGTCGACGCGAAAGCGGCGCTGTCCTTGCTCACCGACGCCGCCAGCATGCAG
CGTCTGAACGAGGCAGTGCCCGCGAACAGATGA

Upstream 100 bases:

>100_bases
CGTGCGGCGCCGGTTGGCGCGTCGATTTGAAGAACTTCCTCACCTCCGACGAACTCGCGCAGTTCGCCGCAAGGCTCAAC
AACTAAACGGCACCACCAAC

Downstream 100 bases:

>100_bases
TCGCCACCTGGTGGGCCCGTCTCTTCAATCGGGAACCTCTGCGCCGAATTTGGAGGCTACCAGCGCCCGCATCCCAGCAA
CGAGTGGGTCCGGGCCCTCG

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKSIIDNDTFGRFVVQTFHQGEGKLYTDAYIQPGQPVRVRLGSHRWADLEYIVGDQKEKRNLVFSAEDFDALMANLFQKL
THRAGEPARKWQDLVAQSNGCLHPMITLSAPSPTGDTTFRVRFTLQRQDMGGYGLMLRCLRPVPESIEELGLPSTVHALC
QAGHGLVLVTGRTGAGKSTTTAALIHQINRSTTSNIVVIEEPIEFIHRPDKARFTYREVGVDVDTYANGVEQVLRYVPDV
ISIGEIRDPETMRAAVRAAESGHLVFGTIHAANTTGAIRKALGYLDSPGEKLSFATNLVGVIAQALLPAKTGTGKALAFE
VMDFTTKRSGTTPLAETVERMLSAKSDGTELRQFETDFLSSSNLGMGNSPFIQSVVTLVRKGHVDAKAALSLLTDAASMQ
RLNEAVPANR

Sequences:

>Translated_410_residues
MKSIIDNDTFGRFVVQTFHQGEGKLYTDAYIQPGQPVRVRLGSHRWADLEYIVGDQKEKRNLVFSAEDFDALMANLFQKL
THRAGEPARKWQDLVAQSNGCLHPMITLSAPSPTGDTTFRVRFTLQRQDMGGYGLMLRCLRPVPESIEELGLPSTVHALC
QAGHGLVLVTGRTGAGKSTTTAALIHQINRSTTSNIVVIEEPIEFIHRPDKARFTYREVGVDVDTYANGVEQVLRYVPDV
ISIGEIRDPETMRAAVRAAESGHLVFGTIHAANTTGAIRKALGYLDSPGEKLSFATNLVGVIAQALLPAKTGTGKALAFE
VMDFTTKRSGTTPLAETVERMLSAKSDGTELRQFETDFLSSSNLGMGNSPFIQSVVTLVRKGHVDAKAALSLLTDAASMQ
RLNEAVPANR
>Mature_410_residues
MKSIIDNDTFGRFVVQTFHQGEGKLYTDAYIQPGQPVRVRLGSHRWADLEYIVGDQKEKRNLVFSAEDFDALMANLFQKL
THRAGEPARKWQDLVAQSNGCLHPMITLSAPSPTGDTTFRVRFTLQRQDMGGYGLMLRCLRPVPESIEELGLPSTVHALC
QAGHGLVLVTGRTGAGKSTTTAALIHQINRSTTSNIVVIEEPIEFIHRPDKARFTYREVGVDVDTYANGVEQVLRYVPDV
ISIGEIRDPETMRAAVRAAESGHLVFGTIHAANTTGAIRKALGYLDSPGEKLSFATNLVGVIAQALLPAKTGTGKALAFE
VMDFTTKRSGTTPLAETVERMLSAKSDGTELRQFETDFLSSSNLGMGNSPFIQSVVTLVRKGHVDAKAALSLLTDAASMQ
RLNEAVPANR

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=247, Percent_Identity=34.0080971659919, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789723, Length=167, Percent_Identity=36.5269461077844, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786296, Length=185, Percent_Identity=35.1351351351351, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 44712; Mature: 44712

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIIDNDTFGRFVVQTFHQGEGKLYTDAYIQPGQPVRVRLGSHRWADLEYIVGDQKEKR
CCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCCCCCCCCEEECCCHHHC
NLVFSAEDFDALMANLFQKLTHRAGEPARKWQDLVAQSNGCLHPMITLSAPSPTGDTTFR
CEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEE
VRFTLQRQDMGGYGLMLRCLRPVPESIEELGLPSTVHALCQAGHGLVLVTGRTGAGKSTT
EEEEEEECCCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCHH
TAALIHQINRSTTSNIVVIEEPIEFIHRPDKARFTYREVGVDVDTYANGVEQVLRYVPDV
HHHHHHHHHCCCCCCEEEEECCHHHHCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHCCCH
ISIGEIRDPETMRAAVRAAESGHLVFGTIHAANTTGAIRKALGYLDSPGEKLSFATNLVG
HCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
VIAQALLPAKTGTGKALAFEVMDFTTKRSGTTPLAETVERMLSAKSDGTELRQFETDFLS
HHHHHHHCCCCCCCCEEEEHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHC
SSNLGMGNSPFIQSVVTLVRKGHVDAKAALSLLTDAASMQRLNEAVPANR
CCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKSIIDNDTFGRFVVQTFHQGEGKLYTDAYIQPGQPVRVRLGSHRWADLEYIVGDQKEKR
CCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCEEEEECCCCCCCCCEEECCCHHHC
NLVFSAEDFDALMANLFQKLTHRAGEPARKWQDLVAQSNGCLHPMITLSAPSPTGDTTFR
CEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCEEE
VRFTLQRQDMGGYGLMLRCLRPVPESIEELGLPSTVHALCQAGHGLVLVTGRTGAGKSTT
EEEEEEECCCCCHHHHHHHHHCCHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCHH
TAALIHQINRSTTSNIVVIEEPIEFIHRPDKARFTYREVGVDVDTYANGVEQVLRYVPDV
HHHHHHHHHCCCCCCEEEEECCHHHHCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHCCCH
ISIGEIRDPETMRAAVRAAESGHLVFGTIHAANTTGAIRKALGYLDSPGEKLSFATNLVG
HCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHH
VIAQALLPAKTGTGKALAFEVMDFTTKRSGTTPLAETVERMLSAKSDGTELRQFETDFLS
HHHHHHHCCCCCCCCEEEEHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHC
SSNLGMGNSPFIQSVVTLVRKGHVDAKAALSLLTDAASMQRLNEAVPANR
CCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]