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The map label for this gene is gloB [H]

Identifier: 120611319

GI number: 120611319

Start: 2913264

End: 2914040

Strand: Reverse

Name: gloB [H]

Synonym: Aave_2651

Alternate gene names: 120611319

Gene position: 2914040-2913264 (Counterclockwise)

Preceding gene: 120611322

Following gene: 120611318

Centisome position: 54.44

GC content: 73.23

Gene sequence:

>777_bases
TTGCTGCCGCTGCCCGCTTTCGCCGACAACTACATCTGGATGCTGCACGACGGCCACTCGGCCGCCGTGGTCGATCCAGG
GGAGGCCGGCCCGGTGCTGCAGGCCCTGCAGCACCATGGCCTGGCACTGCAGGCGATTCTAGTCACGCACCACCACGGCG
ATCATGTCGGCGGCGTGGCGGCGCTGCGCGAGGCTACCGGCGCACCCGTGTACGGCCCGGCGCGCGAATCCATTCCCCAG
CCGGCGCAGCCGCTGGCCGATGGCGACACGCTGCAGGTGCTGGGCCTGCGCCTGTCCGTGATCGACGTGCCCGGCCACAC
GGCGGGGCACATCGCCTACCACGCCGCGGACGCCGCGGGCGGCCCGCTGCTCTTCTGCGGCGACACGCTGTTTTCCGGCG
GGTGCGGCCGGCTCTTCGAGGGCACCCCCGCCCAGATGCAGCATTCGCTCGACCGGCTCGGGGCGCTGCCGCCGGAGACG
CGGGTGTGCTGCGCCCACGAATACACACTCTCGAACCTCGCTTTCGCGCGGGCCGTGGAACCCGGCAACGCGGCGCTGCT
CCAGTACAGCCATGACTGCGAGGCGCTCCGCGCCGCCGGGCAGCCCACCCTGCCCTCGCGGCTCGGCACGGAGCATGCCG
TGAACCCCTTCCTGCGGGCGCGGGAGCCCGCCGTGGCCCGCGCGGCGCAGGGCTTCGACCCGCGTACCGACGCCGCCGAC
CCGGCCTCGGTGCTGGCGGCGCTGCGCCAGTGGAAAAACGAATTCCGACCGACCTGA

Upstream 100 bases:

>100_bases
CACCATCTCGTCGTAGCGCTCCTGCTCCCACGCCAGCAGGTAGCGGCCGGGCGGTGAGTCGAACCAATGGTGCAAACCTA
TAATTTCGTCGCTCATGAAC

Downstream 100 bases:

>100_bases
CCCCATGAAACTACTGCACATCCTGGGCCTCGCGAGCGTGCTCTGGCTCACCGGCTGCGCGGCGCCCGGCACCCCCGGCA
AGGACGCCCCGGCCACCCCC

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLPLPAFADNYIWMLHDGHSAAVVDPGEAGPVLQALQHHGLALQAILVTHHHGDHVGGVAALREATGAPVYGPARESIPQ
PAQPLADGDTLQVLGLRLSVIDVPGHTAGHIAYHAADAAGGPLLFCGDTLFSGGCGRLFEGTPAQMQHSLDRLGALPPET
RVCCAHEYTLSNLAFARAVEPGNAALLQYSHDCEALRAAGQPTLPSRLGTEHAVNPFLRAREPAVARAAQGFDPRTDAAD
PASVLAALRQWKNEFRPT

Sequences:

>Translated_258_residues
MLPLPAFADNYIWMLHDGHSAAVVDPGEAGPVLQALQHHGLALQAILVTHHHGDHVGGVAALREATGAPVYGPARESIPQ
PAQPLADGDTLQVLGLRLSVIDVPGHTAGHIAYHAADAAGGPLLFCGDTLFSGGCGRLFEGTPAQMQHSLDRLGALPPET
RVCCAHEYTLSNLAFARAVEPGNAALLQYSHDCEALRAAGQPTLPSRLGTEHAVNPFLRAREPAVARAAQGFDPRTDAAD
PASVLAALRQWKNEFRPT
>Mature_258_residues
MLPLPAFADNYIWMLHDGHSAAVVDPGEAGPVLQALQHHGLALQAILVTHHHGDHVGGVAALREATGAPVYGPARESIPQ
PAQPLADGDTLQVLGLRLSVIDVPGHTAGHIAYHAADAAGGPLLFCGDTLFSGGCGRLFEGTPAQMQHSLDRLGALPPET
RVCCAHEYTLSNLAFARAVEPGNAALLQYSHDCEALRAAGQPTLPSRLGTEHAVNPFLRAREPAVARAAQGFDPRTDAAD
PASVLAALRQWKNEFRPT

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=259, Percent_Identity=40.9266409266409, Blast_Score=191, Evalue=5e-49,
Organism=Homo sapiens, GI94538322, Length=259, Percent_Identity=40.9266409266409, Blast_Score=190, Evalue=9e-49,
Organism=Homo sapiens, GI14150041, Length=257, Percent_Identity=38.9105058365759, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI116642887, Length=225, Percent_Identity=38.6666666666667, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI21703352, Length=225, Percent_Identity=38.6666666666667, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI46361987, Length=185, Percent_Identity=41.6216216216216, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1786406, Length=254, Percent_Identity=46.8503937007874, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1787158, Length=160, Percent_Identity=37.5, Blast_Score=86, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536925, Length=256, Percent_Identity=35.15625, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6320478, Length=270, Percent_Identity=32.2222222222222, Blast_Score=113, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324614, Length=276, Percent_Identity=32.6086956521739, Blast_Score=96, Evalue=5e-21,
Organism=Drosophila melanogaster, GI21356335, Length=260, Percent_Identity=41.5384615384615, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24667711, Length=260, Percent_Identity=41.5384615384615, Blast_Score=193, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24667703, Length=260, Percent_Identity=41.5384615384615, Blast_Score=192, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 27138; Mature: 27138

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00743 BETA_LACTAMASE_B_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPLPAFADNYIWMLHDGHSAAVVDPGEAGPVLQALQHHGLALQAILVTHHHGDHVGGVA
CCCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCEEEEEEEEECCCCCHHHHH
ALREATGAPVYGPARESIPQPAQPLADGDTLQVLGLRLSVIDVPGHTAGHIAYHAADAAG
HHHHHCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCEEEEECCCCC
GPLLFCGDTLFSGGCGRLFEGTPAQMQHSLDRLGALPPETRVCCAHEYTLSNLAFARAVE
CCEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCC
PGNAALLQYSHDCEALRAAGQPTLPSRLGTEHAVNPFLRAREPAVARAAQGFDPRTDAAD
CCCEEEEEECCCHHHHHHCCCCCCCHHCCCHHHCCHHHHCCCHHHHHHHCCCCCCCCCCC
PASVLAALRQWKNEFRPT
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLPLPAFADNYIWMLHDGHSAAVVDPGEAGPVLQALQHHGLALQAILVTHHHGDHVGGVA
CCCCCCCCCCEEEEEECCCEEEEECCCCCCHHHHHHHHCCCEEEEEEEEECCCCCHHHHH
ALREATGAPVYGPARESIPQPAQPLADGDTLQVLGLRLSVIDVPGHTAGHIAYHAADAAG
HHHHHCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCEEEEECCCCC
GPLLFCGDTLFSGGCGRLFEGTPAQMQHSLDRLGALPPETRVCCAHEYTLSNLAFARAVE
CCEEEECCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCC
PGNAALLQYSHDCEALRAAGQPTLPSRLGTEHAVNPFLRAREPAVARAAQGFDPRTDAAD
CCCEEEEEECCCHHHHHHCCCCCCCHHCCCHHHCCHHHHCCCHHHHHHHCCCCCCCCCCC
PASVLAALRQWKNEFRPT
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA