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The map label for this gene is mltD [H]

Identifier: 120611318

GI number: 120611318

Start: 2911715

End: 2913259

Strand: Reverse

Name: mltD [H]

Synonym: Aave_2650

Alternate gene names: 120611318

Gene position: 2913259-2911715 (Counterclockwise)

Preceding gene: 120611319

Following gene: 120611311

Centisome position: 54.43

GC content: 69.32

Gene sequence:

>1545_bases
ATGAAACTACTGCACATCCTGGGCCTCGCGAGCGTGCTCTGGCTCACCGGCTGCGCGGCGCCCGGCACCCCCGGCAAGGA
CGCCCCGGCCACCCCCTCCAGCACGACAGGGGGCACCACGCCCACCCCGCCCACGGCCGGCTCCCCTCCTCTGCGTCCCA
TCACCGCCGGGGACGCCAGCCACTTCGGCGTGACCTCGCTGGCCGCGCCCACCGACCTGTGGGACCGCATCCGCCGCGGC
TTCTCCATGCCGGACCTCGACCAGGACCTGGTGCGCGACCGCGAGCTCTGGTATGCGAGCCGCCCCGACTACATGCAGCG
CATGACCGAGCGCTCCAGCAAGTACCTCTTCCACATCGTGGAGGAACTGGAGTTGCGCGGCATGCCCACCGAACTGGCGC
TGCTGCCCTATATCGAGAGCGCCTTCAACCCCATGGCCGTCTCCAGTGCCAAGGCCGTGGGCATGTGGCAGTTCATGCCC
GCCACGGGCAGCGACTACCAGCTCAAGCAGAACGCCTTCCGCGACGACCGGCGCGACGTGCTCGCCTCCACCCGCGCGGC
ACTGGACTACCTGCAGCGCCTCTACGGCACGTTCGGCGACTGGCACCTGGCCCTGGCCGCCTACAACTGGGGCGAAGGCA
ACGTGAGCCGCGCCATCGCCCGCAACCAGAAGATGGGCCTGGGCACCAGCTACACCGAGCTGAACATGCCGGCCGAAACC
CGCATGTACGTGCCCAAGCTGCAGGCGGTGAAGAACATCGTCGCGCACCCGGAGCGGTTCAACACCGAGCTGCCGCTCAT
CGAGAACCACCCCTATTTCCAGACAGTGGACATCACCCGCGACATCGACGTCTCGCTGGTGGCCAGCCTGGCGGGCGTGC
GCGAACAGGACTTCCGCGAGCTCAACCCGTCTCTGCACAAGCCCATCATCCTCGCGGCCGGCATGCCGCAGATCCTGCTG
CCCTGGGACAACGCACAGGTCTTCCGCAAGAACCTGGAGGCCTACACCGAGGGCCAGTACGCGAGCTGGACGGTCTGGAG
CGTGCCCACCACGATGAGCGTGTCGGCGGCGGCGCAGCGCGTCGGCATGAGCGAGACCGACCTGCGCAACGTGAACAAGA
TCCCGCCGCGCATGCTCATCAAGGCCGGGTCGGCCCTGATGGTGCCCCGCAGCAACACGACGCAGGCCGACGTGCCCGGG
CAGCTCGCCGACAACGGCCAGGTCGCCTTCACCCCCGAGATCGTCACCCGCCGCACCACGGTGCGCGCCGGCAAGCGCGA
CTCGGTGGCCACCATCGCGCGGCGCTACAAAGTCACCACCGCGGACGTCGCGACCTGGAACGACGTGAAATCCACGGCCT
CCTTCAAGGCCGGCGAGGCGGTGGTCGTGTATCTGCCCGTGCGCATGACGTCCGGCGCCTCGGCCAGGAGCAGCAGCCGG
AGCAGCCGGCAGTCGGCGGCTCCGTCCAGGAGCACGGCGAAGAAGGCCTCGGCGCCCGCGCCGTCCCGCAAGGGCGGAAC
CCCGTCGCGCGTGAAGAAGCGCTGA

Upstream 100 bases:

>100_bases
GCGGCGCAGGGCTTCGACCCGCGTACCGACGCCGCCGACCCGGCCTCGGTGCTGGCGGCGCTGCGCCAGTGGAAAAACGA
ATTCCGACCGACCTGACCCC

Downstream 100 bases:

>100_bases
CGGGAAAACGCTGGCGGGCGCCGTCCGCGCCCGTCAGATCCTCAGGTTGTGCATGCCCACCAGCCCGAGCACGCCGATGA
GGATGAGGTAGAGCGCCACG

Product: lytic transglycosylase catalytic subunit

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKLLHILGLASVLWLTGCAAPGTPGKDAPATPSSTTGGTTPTPPTAGSPPLRPITAGDASHFGVTSLAAPTDLWDRIRRG
FSMPDLDQDLVRDRELWYASRPDYMQRMTERSSKYLFHIVEELELRGMPTELALLPYIESAFNPMAVSSAKAVGMWQFMP
ATGSDYQLKQNAFRDDRRDVLASTRAALDYLQRLYGTFGDWHLALAAYNWGEGNVSRAIARNQKMGLGTSYTELNMPAET
RMYVPKLQAVKNIVAHPERFNTELPLIENHPYFQTVDITRDIDVSLVASLAGVREQDFRELNPSLHKPIILAAGMPQILL
PWDNAQVFRKNLEAYTEGQYASWTVWSVPTTMSVSAAAQRVGMSETDLRNVNKIPPRMLIKAGSALMVPRSNTTQADVPG
QLADNGQVAFTPEIVTRRTTVRAGKRDSVATIARRYKVTTADVATWNDVKSTASFKAGEAVVVYLPVRMTSGASARSSSR
SSRQSAAPSRSTAKKASAPAPSRKGGTPSRVKKR

Sequences:

>Translated_514_residues
MKLLHILGLASVLWLTGCAAPGTPGKDAPATPSSTTGGTTPTPPTAGSPPLRPITAGDASHFGVTSLAAPTDLWDRIRRG
FSMPDLDQDLVRDRELWYASRPDYMQRMTERSSKYLFHIVEELELRGMPTELALLPYIESAFNPMAVSSAKAVGMWQFMP
ATGSDYQLKQNAFRDDRRDVLASTRAALDYLQRLYGTFGDWHLALAAYNWGEGNVSRAIARNQKMGLGTSYTELNMPAET
RMYVPKLQAVKNIVAHPERFNTELPLIENHPYFQTVDITRDIDVSLVASLAGVREQDFRELNPSLHKPIILAAGMPQILL
PWDNAQVFRKNLEAYTEGQYASWTVWSVPTTMSVSAAAQRVGMSETDLRNVNKIPPRMLIKAGSALMVPRSNTTQADVPG
QLADNGQVAFTPEIVTRRTTVRAGKRDSVATIARRYKVTTADVATWNDVKSTASFKAGEAVVVYLPVRMTSGASARSSSR
SSRQSAAPSRSTAKKASAPAPSRKGGTPSRVKKR
>Mature_514_residues
MKLLHILGLASVLWLTGCAAPGTPGKDAPATPSSTTGGTTPTPPTAGSPPLRPITAGDASHFGVTSLAAPTDLWDRIRRG
FSMPDLDQDLVRDRELWYASRPDYMQRMTERSSKYLFHIVEELELRGMPTELALLPYIESAFNPMAVSSAKAVGMWQFMP
ATGSDYQLKQNAFRDDRRDVLASTRAALDYLQRLYGTFGDWHLALAAYNWGEGNVSRAIARNQKMGLGTSYTELNMPAET
RMYVPKLQAVKNIVAHPERFNTELPLIENHPYFQTVDITRDIDVSLVASLAGVREQDFRELNPSLHKPIILAAGMPQILL
PWDNAQVFRKNLEAYTEGQYASWTVWSVPTTMSVSAAAQRVGMSETDLRNVNKIPPRMLIKAGSALMVPRSNTTQADVPG
QLADNGQVAFTPEIVTRRTTVRAGKRDSVATIARRYKVTTADVATWNDVKSTASFKAGEAVVVYLPVRMTSGASARSSSR
SSRQSAAPSRSTAKKASAPAPSRKGGTPSRVKKR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=479, Percent_Identity=32.3590814196242, Blast_Score=221, Evalue=7e-59,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 56328; Mature: 56328

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLHILGLASVLWLTGCAAPGTPGKDAPATPSSTTGGTTPTPPTAGSPPLRPITAGDAS
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCC
HFGVTSLAAPTDLWDRIRRGFSMPDLDQDLVRDRELWYASRPDYMQRMTERSSKYLFHIV
CCCCHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EELELRGMPTELALLPYIESAFNPMAVSSAKAVGMWQFMPATGSDYQLKQNAFRDDRRDV
HHHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
LASTRAALDYLQRLYGTFGDWHLALAAYNWGEGNVSRAIARNQKMGLGTSYTELNMPAET
HHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCHHH
RMYVPKLQAVKNIVAHPERFNTELPLIENHPYFQTVDITRDIDVSLVASLAGVREQDFRE
HHHHHHHHHHHHHHCCCHHCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHCCHHHHHHH
LNPSLHKPIILAAGMPQILLPWDNAQVFRKNLEAYTEGQYASWTVWSVPTTMSVSAAAQR
CCHHHCCCEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHH
VGMSETDLRNVNKIPPRMLIKAGSALMVPRSNTTQADVPGQLADNGQVAFTPEIVTRRTT
CCCCHHHHHHHHCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEECHHHHHHHHH
VRAGKRDSVATIARRYKVTTADVATWNDVKSTASFKAGEAVVVYLPVRMTSGASARSSSR
HCCCCCCHHHHHHHHHCCEECCCCCCHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCH
SSRQSAAPSRSTAKKASAPAPSRKGGTPSRVKKR
HHHHHCCCCHHHHHHCCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure
MKLLHILGLASVLWLTGCAAPGTPGKDAPATPSSTTGGTTPTPPTAGSPPLRPITAGDAS
CHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCC
HFGVTSLAAPTDLWDRIRRGFSMPDLDQDLVRDRELWYASRPDYMQRMTERSSKYLFHIV
CCCCHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
EELELRGMPTELALLPYIESAFNPMAVSSAKAVGMWQFMPATGSDYQLKQNAFRDDRRDV
HHHHHCCCCHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHH
LASTRAALDYLQRLYGTFGDWHLALAAYNWGEGNVSRAIARNQKMGLGTSYTELNMPAET
HHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCHHH
RMYVPKLQAVKNIVAHPERFNTELPLIENHPYFQTVDITRDIDVSLVASLAGVREQDFRE
HHHHHHHHHHHHHHCCCHHCCCCCCEEECCCCEEEEECCCCCCHHHHHHHHCCHHHHHHH
LNPSLHKPIILAAGMPQILLPWDNAQVFRKNLEAYTEGQYASWTVWSVPTTMSVSAAAQR
CCHHHCCCEEEECCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHH
VGMSETDLRNVNKIPPRMLIKAGSALMVPRSNTTQADVPGQLADNGQVAFTPEIVTRRTT
CCCCHHHHHHHHCCCHHHHHHCCCEEEECCCCCCCCCCCCCCCCCCCEEECHHHHHHHHH
VRAGKRDSVATIARRYKVTTADVATWNDVKSTASFKAGEAVVVYLPVRMTSGASARSSSR
HCCCCCCHHHHHHHHHCCEECCCCCCHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCCCH
SSRQSAAPSRSTAKKASAPAPSRKGGTPSRVKKR
HHHHHCCCCHHHHHHCCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]