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The map label for this gene is phoB [H]

Identifier: 120611299

GI number: 120611299

Start: 2884446

End: 2885153

Strand: Direct

Name: phoB [H]

Synonym: Aave_2631

Alternate gene names: 120611299

Gene position: 2884446-2885153 (Clockwise)

Preceding gene: 120611298

Following gene: 120611300

Centisome position: 53.89

GC content: 68.79

Gene sequence:

>708_bases
ATGAAGAGACTTCCCCGCGTACTCATCGTCGAAGACGAATCCGCCATCGCCGAGCTGATCGCCGTCAACCTGCGCCACAA
CGGCTTCCAGCCCATCTGGTCCGAGGACGGCGAGTCCGCCCAGCGCGAGCTGGATGCGGTCCTGCCCGACGTCATCCTGC
TCGACTGGATGCTGCCCGGCCAGAGCGGCCTGCAGCTCGCGCGCAAGTGGCGCGCGGACGCGCGCACCAAGCCCATTCCC
ATCCTCATGCTCACGGCGCGTGGCGACGAGCCCGACAAGGTGGCCGGGCTCGATGCGGGCGCGGACGACTACATCACCAA
GCCGTTCTCCACCCAGGAGCTGCTGGCGCGCATCCGCGCCGTGCTGCGGCGCCGGGCGCCCGAGCAGGCCACCGATACCG
TGGCCATTTCCGACCTGATCCTCGACGCCGGCACCTACCGCGTGACTTACCAGGGGCAGCAGATCAAGATCGGCCCGACC
GAGTTCAAGCTGCTGCACTACCTCATGAAGCACCCGGAACGCGTCCACAGCCGGGCACAGCTGCTCGACAAGGTGTGGGG
CGACCACGTCTTCATCGAGGAGCGCACGGTGGACGTTCACGTCAAGCGCCTGCGCGAAGCCCTGGGCGTCGCCGGCGCCA
TGGTGGAAACCGTGCGCGGCGCGGGCTACCGGCTCACGGCCCAGCCTGCCGCCCTGATGCGGGCCTGA

Upstream 100 bases:

>100_bases
GTGGCCGAGCTCATCATCTACCTCGTCAAGGGCAAGGACGTGCGCCACACGGCCCTCGAGGAGATCGAATCGGCCGTGCT
CTGAAAGGCCACCCCCTCCC

Downstream 100 bases:

>100_bases
CCGTCGAGCGGGCCGCCCGCGGGCGGCCTTCCTTTTCGTTATCTGACAACAAGGCAGCGGAGCGCGGACGGGGAGGGGCC
CCGCGGAGCCCTCCCGGATT

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKRLPRVLIVEDESAIAELIAVNLRHNGFQPIWSEDGESAQRELDAVLPDVILLDWMLPGQSGLQLARKWRADARTKPIP
ILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRAVLRRRAPEQATDTVAISDLILDAGTYRVTYQGQQIKIGPT
EFKLLHYLMKHPERVHSRAQLLDKVWGDHVFIEERTVDVHVKRLREALGVAGAMVETVRGAGYRLTAQPAALMRA

Sequences:

>Translated_235_residues
MKRLPRVLIVEDESAIAELIAVNLRHNGFQPIWSEDGESAQRELDAVLPDVILLDWMLPGQSGLQLARKWRADARTKPIP
ILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRAVLRRRAPEQATDTVAISDLILDAGTYRVTYQGQQIKIGPT
EFKLLHYLMKHPERVHSRAQLLDKVWGDHVFIEERTVDVHVKRLREALGVAGAMVETVRGAGYRLTAQPAALMRA
>Mature_235_residues
MKRLPRVLIVEDESAIAELIAVNLRHNGFQPIWSEDGESAQRELDAVLPDVILLDWMLPGQSGLQLARKWRADARTKPIP
ILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRAVLRRRAPEQATDTVAISDLILDAGTYRVTYQGQQIKIGPT
EFKLLHYLMKHPERVHSRAQLLDKVWGDHVFIEERTVDVHVKRLREALGVAGAMVETVRGAGYRLTAQPAALMRA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=225, Percent_Identity=52.8888888888889, Blast_Score=253, Evalue=7e-69,
Organism=Escherichia coli, GI1788394, Length=227, Percent_Identity=40.0881057268723, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=36.5638766519824, Blast_Score=139, Evalue=1e-34,
Organism=Escherichia coli, GI1790860, Length=223, Percent_Identity=37.6681614349776, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=36.036036036036, Blast_Score=130, Evalue=5e-32,
Organism=Escherichia coli, GI1789402, Length=220, Percent_Identity=35.4545454545455, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787375, Length=220, Percent_Identity=33.6363636363636, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1786911, Length=229, Percent_Identity=35.8078602620087, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI2367329, Length=234, Percent_Identity=34.1880341880342, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=31.6239316239316, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=35.2678571428571, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI87082012, Length=227, Percent_Identity=33.920704845815, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1787229, Length=228, Percent_Identity=29.3859649122807, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI145693140, Length=233, Percent_Identity=30.4721030042918, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=30, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788550, Length=115, Percent_Identity=30.4347826086957, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26327; Mature: 26327

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLPRVLIVEDESAIAELIAVNLRHNGFQPIWSEDGESAQRELDAVLPDVILLDWMLPG
CCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
QSGLQLARKWRADARTKPIPILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRA
CHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHH
VLRRRAPEQATDTVAISDLILDAGTYRVTYQGQQIKIGPTEFKLLHYLMKHPERVHSRAQ
HHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEEECCHHHHHHHHHHHCHHHHHHHHH
LLDKVWGDHVFIEERTVDVHVKRLREALGVAGAMVETVRGAGYRLTAQPAALMRA
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCC
>Mature Secondary Structure
MKRLPRVLIVEDESAIAELIAVNLRHNGFQPIWSEDGESAQRELDAVLPDVILLDWMLPG
CCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
QSGLQLARKWRADARTKPIPILMLTARGDEPDKVAGLDAGADDYITKPFSTQELLARIRA
CHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHH
VLRRRAPEQATDTVAISDLILDAGTYRVTYQGQQIKIGPTEFKLLHYLMKHPERVHSRAQ
HHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCEEEECCHHHHHHHHHHHCHHHHHHHHH
LLDKVWGDHVFIEERTVDVHVKRLREALGVAGAMVETVRGAGYRLTAQPAALMRA
HHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]