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The map label for this gene is lutR [H]

Identifier: 120611257

GI number: 120611257

Start: 2835372

End: 2836184

Strand: Reverse

Name: lutR [H]

Synonym: Aave_2589

Alternate gene names: 120611257

Gene position: 2836184-2835372 (Counterclockwise)

Preceding gene: 120611266

Following gene: 120611256

Centisome position: 52.99

GC content: 70.6

Gene sequence:

>813_bases
ATGGCCGCCTCCACAAAGGGATTACCCTCACCCACCGCCCCCGCCGACCGACATGCGCCACCCGCACTGCCGGCGCGGCG
TGCCCGGGGCCTGGCCCATGCGGTGGTGGAGGATTTCTCGGGCAGGATCCGCGACCGGGCCCTCGTGCCGGGCGACAAGC
TGCCCACGGAATCCGAGATCATGCGCGCGCACGATGTCAGCCGCACGGTGGTGCGCGAGGCGATCTCCCGGCTGCAGGCC
GCCGGCCTCGTGGAGACCCGCCACGGGATCGGGACCTTCGTGCTGCCGCCGCGCGCGGGCGGAGGCTTCCGGCTCGATCC
ATCCGAGCTCAGCACGTCCGCCGACGTGCTCGCCGTGCTGGAGTTGCGCATCAGCCTCGAAACCGAATCCGCGGGTCTGG
CCGCCATGCGGCGCAGCGATACGCACCTGCAGGCCATGGCGCAGGCTCTGGACGATTTCGAGCGCAACGTCCGCAGCGGC
GGAGACACGGTGGCGCACGACTTCCGCTTCCACCTGCAGATCGCCGAGGCCACGGGCAATCCGTACTTCGCGGACATCAT
GAACCACCTCGGCACCACCCTGATACCCCGCACCCGCATCGCGGCCCTGCGGGTGCCGCGCAGCACCGAGTACCTGGACC
GGGTGAACCGCGAGCACGAAGAGATCTACGCCGCCATCGCGCGGCAGGACCCCGATTCCGCGCGTGCGGCCATGCGCATC
CATTTGACGAACAGCCGCGAACGCCTGCGACTGGCCCAGGAGGCGGCCAGGAACCAGGCCGGCTCCGAGACTCCGTCGCC
ACCTTCCGCCTAG

Upstream 100 bases:

>100_bases
AGCAACAGTCGGTGGAATCGGGAAGGCTGGCTCATGGCGTTTTGTGTGCGGGTATTTGTCATATGTTGTCGTACAACGCG
ACGGGATTATGATCACCGCC

Downstream 100 bases:

>100_bases
CGAAACGGGCAGGGATGACCGCCATTGTTCTTCAGGCGGGCACAGTTGTATGATGACGTACAACATAAGGAGACAGAGAC
ATGCCCTTTTCCCGCCGCGC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MAASTKGLPSPTAPADRHAPPALPARRARGLAHAVVEDFSGRIRDRALVPGDKLPTESEIMRAHDVSRTVVREAISRLQA
AGLVETRHGIGTFVLPPRAGGGFRLDPSELSTSADVLAVLELRISLETESAGLAAMRRSDTHLQAMAQALDDFERNVRSG
GDTVAHDFRFHLQIAEATGNPYFADIMNHLGTTLIPRTRIAALRVPRSTEYLDRVNREHEEIYAAIARQDPDSARAAMRI
HLTNSRERLRLAQEAARNQAGSETPSPPSA

Sequences:

>Translated_270_residues
MAASTKGLPSPTAPADRHAPPALPARRARGLAHAVVEDFSGRIRDRALVPGDKLPTESEIMRAHDVSRTVVREAISRLQA
AGLVETRHGIGTFVLPPRAGGGFRLDPSELSTSADVLAVLELRISLETESAGLAAMRRSDTHLQAMAQALDDFERNVRSG
GDTVAHDFRFHLQIAEATGNPYFADIMNHLGTTLIPRTRIAALRVPRSTEYLDRVNREHEEIYAAIARQDPDSARAAMRI
HLTNSRERLRLAQEAARNQAGSETPSPPSA
>Mature_269_residues
AASTKGLPSPTAPADRHAPPALPARRARGLAHAVVEDFSGRIRDRALVPGDKLPTESEIMRAHDVSRTVVREAISRLQAA
GLVETRHGIGTFVLPPRAGGGFRLDPSELSTSADVLAVLELRISLETESAGLAAMRRSDTHLQAMAQALDDFERNVRSGG
DTVAHDFRFHLQIAEATGNPYFADIMNHLGTTLIPRTRIAALRVPRSTEYLDRVNREHEEIYAAIARQDPDSARAAMRIH
LTNSRERLRLAQEAARNQAGSETPSPPSA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790780, Length=219, Percent_Identity=32.4200913242009, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI87082208, Length=218, Percent_Identity=29.8165137614679, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789621, Length=223, Percent_Identity=30.0448430493274, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790032, Length=221, Percent_Identity=27.6018099547511, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786303, Length=223, Percent_Identity=28.6995515695067, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 29377; Mature: 29246

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAASTKGLPSPTAPADRHAPPALPARRARGLAHAVVEDFSGRIRDRALVPGDKLPTESEI
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
MRAHDVSRTVVREAISRLQAAGLVETRHGIGTFVLPPRAGGGFRLDPSELSTSADVLAVL
HHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEECCCCCCCCEECHHHHCCHHHHHHHH
ELRISLETESAGLAAMRRSDTHLQAMAQALDDFERNVRSGGDTVAHDFRFHLQIAEATGN
HHHHEECCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEEEEEEECCCC
PYFADIMNHLGTTLIPRTRIAALRVPRSTEYLDRVNREHEEIYAAIARQDPDSARAAMRI
CHHHHHHHHHCCHHCCCHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHCCCCHHHEEEEE
HLTNSRERLRLAQEAARNQAGSETPSPPSA
EECCCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
AASTKGLPSPTAPADRHAPPALPARRARGLAHAVVEDFSGRIRDRALVPGDKLPTESEI
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
MRAHDVSRTVVREAISRLQAAGLVETRHGIGTFVLPPRAGGGFRLDPSELSTSADVLAVL
HHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEECCCCCCCCEECHHHHCCHHHHHHHH
ELRISLETESAGLAAMRRSDTHLQAMAQALDDFERNVRSGGDTVAHDFRFHLQIAEATGN
HHHHEECCCCCCHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEEEEEEECCCC
PYFADIMNHLGTTLIPRTRIAALRVPRSTEYLDRVNREHEEIYAAIARQDPDSARAAMRI
CHHHHHHHHHCCHHCCCHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHCCCCHHHEEEEE
HLTNSRERLRLAQEAARNQAGSETPSPPSA
EECCCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]