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The map label for this gene is ampR [H]

Identifier: 120611220

GI number: 120611220

Start: 2800404

End: 2801309

Strand: Reverse

Name: ampR [H]

Synonym: Aave_2551

Alternate gene names: 120611220

Gene position: 2801309-2800404 (Counterclockwise)

Preceding gene: 120611222

Following gene: 120611219

Centisome position: 52.33

GC content: 73.29

Gene sequence:

>906_bases
ATGCACCTGCCGCTCAATGCCCTGCGGGCGTTCGAAGTCTCGGCGCGGCACCTGAATCTCACCCGCGCGGCGGAAGAGCT
GCACGTGACGCAGACCGCGGTGAGCCAGCACATCCGCAACCTCGAGGAGCGCCTGGGCAAGCCGCTGTTCCGCAGGCTGC
CGCGCGGGCTGGCGCTCACCGATGAAGGTCTGGCGCTGCTGCCGGCGCTGGCGGACGCGTTCGGCCGCATCGAGCGCGCG
CTGGCGCAGTGCAGCGACACACGCCCGCGCGAAGTGCTCACCGTGGGCGCCGTGGGCACCTTCGCCGTGGGATGGCTGCT
GCCGCGGCTGCAGGCATTCCAGCAGCAGTGTCCGTTCGTCGATCTGCGCCTGCTCACCAACAACAACCGGGTGGACCTTG
CGGGCGAAGGGCTGGACTGCGCGATCCGTTTCGGCGACGGCGCCTGGCACGGCACGGAATCGGACCGCCTGCTGGAAGCG
CCCCTGACGCCCATGTGCTCGCCCGCGCTCGCCGCCCGGCTGCGCGCGCCGGCCGACTTGGCGCGGGAGTCGCTGCTGCG
CTCGTACCGCGCCGATGAATGGGCCGCCTGGTTCGGTGCCGCGGGCGTGGCCTGCCCCGTGGTTCGGGGTACGGTGTTCG
ACTCGTCCCTGGCCCTGGCCGAAGCGGCGGCGCAGAGCGCGGGCGCGGCCCTGCTTCCCGCGCGCCTGTTCGAGCGCGAA
TTGCGCCAGGGCCGGCTGGTGCGGCCTTTCGGCCACGAGATCGCGCTCGGGGCCTACTGGTTCACGCGCCTGCGGTCGCG
CGCCGCGTCCCCCGCGCTCACGGCCTTCCGGGAATGGCTGCTGCAAGCCTGCGCCGTGGGCGGCGCACCGGTGGAGCCTC
CGCCGTCTGCAGCCATGGCGCCGTAG

Upstream 100 bases:

>100_bases
TCCGTTGTTGAACAATGGCCGCAACGATATCGGTCCGCACGGCCGGTGACCATCGACAATAATTGGCATCTTCCCCCGGA
AAAACTTCACCCTCGCACCC

Downstream 100 bases:

>100_bases
CCCCCCGGCTTATCATCCCCGGCCCATGTCCCAGATCCTGACCGCCGCCCTCTACCACTTCGCGAAGCTGCCCGACTGCG
ATGCGCTGCGCGGTCCCCTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MHLPLNALRAFEVSARHLNLTRAAEELHVTQTAVSQHIRNLEERLGKPLFRRLPRGLALTDEGLALLPALADAFGRIERA
LAQCSDTRPREVLTVGAVGTFAVGWLLPRLQAFQQQCPFVDLRLLTNNNRVDLAGEGLDCAIRFGDGAWHGTESDRLLEA
PLTPMCSPALAARLRAPADLARESLLRSYRADEWAAWFGAAGVACPVVRGTVFDSSLALAEAAAQSAGAALLPARLFERE
LRQGRLVRPFGHEIALGAYWFTRLRSRAASPALTAFREWLLQACAVGGAPVEPPPSAAMAP

Sequences:

>Translated_301_residues
MHLPLNALRAFEVSARHLNLTRAAEELHVTQTAVSQHIRNLEERLGKPLFRRLPRGLALTDEGLALLPALADAFGRIERA
LAQCSDTRPREVLTVGAVGTFAVGWLLPRLQAFQQQCPFVDLRLLTNNNRVDLAGEGLDCAIRFGDGAWHGTESDRLLEA
PLTPMCSPALAARLRAPADLARESLLRSYRADEWAAWFGAAGVACPVVRGTVFDSSLALAEAAAQSAGAALLPARLFERE
LRQGRLVRPFGHEIALGAYWFTRLRSRAASPALTAFREWLLQACAVGGAPVEPPPSAAMAP
>Mature_301_residues
MHLPLNALRAFEVSARHLNLTRAAEELHVTQTAVSQHIRNLEERLGKPLFRRLPRGLALTDEGLALLPALADAFGRIERA
LAQCSDTRPREVLTVGAVGTFAVGWLLPRLQAFQQQCPFVDLRLLTNNNRVDLAGEGLDCAIRFGDGAWHGTESDRLLEA
PLTPMCSPALAARLRAPADLARESLLRSYRADEWAAWFGAAGVACPVVRGTVFDSSLALAEAAAQSAGAALLPARLFERE
LRQGRLVRPFGHEIALGAYWFTRLRSRAASPALTAFREWLLQACAVGGAPVEPPPSAAMAP

Specific function: This protein is a positive regulator of gene expression of beta-lactamase (AmpC) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=287, Percent_Identity=36.2369337979094, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=34.1379310344828, Blast_Score=141, Evalue=6e-35,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=32.9861111111111, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=27.4914089347079, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787128, Length=257, Percent_Identity=27.6264591439689, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786401, Length=285, Percent_Identity=25.6140350877193, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790262, Length=240, Percent_Identity=30, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI145693193, Length=260, Percent_Identity=25, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32620; Mature: 32620

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLPLNALRAFEVSARHLNLTRAAEELHVTQTAVSQHIRNLEERLGKPLFRRLPRGLALT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEC
DEGLALLPALADAFGRIERALAQCSDTRPREVLTVGAVGTFAVGWLLPRLQAFQQQCPFV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCEE
DLRLLTNNNRVDLAGEGLDCAIRFGDGAWHGTESDRLLEAPLTPMCSPALAARLRAPADL
EEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCCCEECCCCCCCCCHHHHHHHCCCHHH
ARESLLRSYRADEWAAWFGAAGVACPVVRGTVFDSSLALAEAAAQSAGAALLPARLFERE
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LRQGRLVRPFGHEIALGAYWFTRLRSRAASPALTAFREWLLQACAVGGAPVEPPPSAAMA
HHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
P
C
>Mature Secondary Structure
MHLPLNALRAFEVSARHLNLTRAAEELHVTQTAVSQHIRNLEERLGKPLFRRLPRGLALT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEC
DEGLALLPALADAFGRIERALAQCSDTRPREVLTVGAVGTFAVGWLLPRLQAFQQQCPFV
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCEE
DLRLLTNNNRVDLAGEGLDCAIRFGDGAWHGTESDRLLEAPLTPMCSPALAARLRAPADL
EEEEEECCCEEEEECCCCEEEEEECCCCCCCCCCCCEECCCCCCCCCHHHHHHHCCCHHH
ARESLLRSYRADEWAAWFGAAGVACPVVRGTVFDSSLALAEAAAQSAGAALLPARLFERE
HHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LRQGRLVRPFGHEIALGAYWFTRLRSRAASPALTAFREWLLQACAVGGAPVEPPPSAAMA
HHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8405939; 10984043; 2125210 [H]