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The map label for this gene is rnfB [H]

Identifier: 120611158

GI number: 120611158

Start: 2721444

End: 2722175

Strand: Reverse

Name: rnfB [H]

Synonym: Aave_2487

Alternate gene names: 120611158

Gene position: 2722175-2721444 (Counterclockwise)

Preceding gene: 120611159

Following gene: 120611156

Centisome position: 50.86

GC content: 75.14

Gene sequence:

>732_bases
TTGACGGCGCCCCCTCCCGCTGCCGCCACGCGCGTCCTGGCGGCCCGGATCGACGCGGCGTTGCCGCAGACTCAGTGCAC
CCGCTGCGGCTACCCCGACTGTGCCGCGTACGCGGATGCGGTCGCCGAAGGAACGGCGGACATCAACCGGTGCCCGCCGG
GCGGAGCCGAAGGCATCGCCCGGCTGGCGGCGCTCACCGGGCGTGCGCCGCTGGCGCTCGACCCGCAGTGCGGCGCCGAA
GCCCCACGCGGCCTCGCCGTGATCGACGAGCTGGCATGCATCGGCTGCACCCTGTGCATCAAGGCCTGCCCCACGGATGC
GATCCTGGGCACCCACAAACGCATGCACACCGTGATCGAGGCCCACTGCACGGGATGCGAGCTCTGCATTCCCGTGTGCC
CGGTGGACTGCATCACGATGGAAAACGCCACTGGCGATGCCACGGGATGGGCCGCGTGGTCCGCCACGCAGGCAGGCCAG
GCCCGGCAGCGCTACCAGGCGCACCGCCTGCGCGCGGCCCCGGACGCGGAGCGGGCCCCAACGGCCTCCGCCCTGGAACC
GGGCCGTGCCACCGCAGCGCCTGCGATCCAGCCCGGGCACGCCCCGTTGCAGGAAGACACCCGCCCCGCGCCGCCGGCCT
CGAGCATCCAGCCGTCCGCCGAAGACCGCCGCGCCGCCATCGCCGCGGCCCTGGCACGCGCCCGCGAGCGCCGGGGCGGC
GGATCGCGATGA

Upstream 100 bases:

>100_bases
CCGGAGCCGCCGCAGCGCCCGCGGACCTGCTGTCCGAGGAACTGCCGACCGCCACCGCCGCACCGCAGACGCGCAGCCGG
CGCAGCGCGCCCAACAAGGC

Downstream 100 bases:

>100_bases
TTACCACGGCGCGGGCACCCGCCGGTACCCGCGCCGCGGTTCATGCCCGGGCCGCGAGCGACTTGTACACGCCGCAGCCC
AGGTACGCGCCCTCCAGTGC

Product: RnfABCDGE type electron transport complex subunit B

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MTAPPPAAATRVLAARIDAALPQTQCTRCGYPDCAAYADAVAEGTADINRCPPGGAEGIARLAALTGRAPLALDPQCGAE
APRGLAVIDELACIGCTLCIKACPTDAILGTHKRMHTVIEAHCTGCELCIPVCPVDCITMENATGDATGWAAWSATQAGQ
ARQRYQAHRLRAAPDAERAPTASALEPGRATAAPAIQPGHAPLQEDTRPAPPASSIQPSAEDRRAAIAAALARARERRGG
GSR

Sequences:

>Translated_243_residues
MTAPPPAAATRVLAARIDAALPQTQCTRCGYPDCAAYADAVAEGTADINRCPPGGAEGIARLAALTGRAPLALDPQCGAE
APRGLAVIDELACIGCTLCIKACPTDAILGTHKRMHTVIEAHCTGCELCIPVCPVDCITMENATGDATGWAAWSATQAGQ
ARQRYQAHRLRAAPDAERAPTASALEPGRATAAPAIQPGHAPLQEDTRPAPPASSIQPSAEDRRAAIAAALARARERRGG
GSR
>Mature_242_residues
TAPPPAAATRVLAARIDAALPQTQCTRCGYPDCAAYADAVAEGTADINRCPPGGAEGIARLAALTGRAPLALDPQCGAEA
PRGLAVIDELACIGCTLCIKACPTDAILGTHKRMHTVIEAHCTGCELCIPVCPVDCITMENATGDATGWAAWSATQAGQA
RQRYQAHRLRAAPDAERAPTASALEPGRATAAPAIQPGHAPLQEDTRPAPPASSIQPSAEDRRAAIAAALARARERRGGG
SR

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=141, Percent_Identity=46.0992907801418, Blast_Score=124, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 24939; Mature: 24808

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00028 ZINC_FINGER_C2H2_1 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
5.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPPPAAATRVLAARIDAALPQTQCTRCGYPDCAAYADAVAEGTADINRCPPGGAEGIA
CCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RLAALTGRAPLALDPQCGAEAPRGLAVIDELACIGCTLCIKACPTDAILGTHKRMHTVIE
HHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AHCTGCELCIPVCPVDCITMENATGDATGWAAWSATQAGQARQRYQAHRLRAAPDAERAP
HHCCCCEEECCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TASALEPGRATAAPAIQPGHAPLQEDTRPAPPASSIQPSAEDRRAAIAAALARARERRGG
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GSR
CCC
>Mature Secondary Structure 
TAPPPAAATRVLAARIDAALPQTQCTRCGYPDCAAYADAVAEGTADINRCPPGGAEGIA
CCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RLAALTGRAPLALDPQCGAEAPRGLAVIDELACIGCTLCIKACPTDAILGTHKRMHTVIE
HHHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
AHCTGCELCIPVCPVDCITMENATGDATGWAAWSATQAGQARQRYQAHRLRAAPDAERAP
HHCCCCEEECCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
TASALEPGRATAAPAIQPGHAPLQEDTRPAPPASSIQPSAEDRRAAIAAALARARERRGG
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
GSR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA