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The map label for this gene is fixJ [H]

Identifier: 120611131

GI number: 120611131

Start: 2683208

End: 2683834

Strand: Reverse

Name: fixJ [H]

Synonym: Aave_2460

Alternate gene names: 120611131

Gene position: 2683834-2683208 (Counterclockwise)

Preceding gene: 120611132

Following gene: 120611130

Centisome position: 50.14

GC content: 64.43

Gene sequence:

>627_bases
ATGAGTTTGACTCCCAAAAAAGGTACTGTCTACGTCGTGGACGACGACGAGGCCGTGCGCGACTCGCTGCAATGGCTGCT
GGAGGGCAAGGACTACCGCGTCCGCTGCTTCGACTCCGCCGAATCCTTCCTCTCACGCTACGATCCACGCGAAGTGGCCT
GCCTGATCGTTGACATCCGCATGGGCGGCATGACCGGGCTCGAACTGCAGGACCGCCTGCTCGAACGCAAGTCCCCCCTG
CCCATCGTCTTCATCACCGGCCATGGCGACGTGCCCATGGCCGTCAACACCATGAAGAAGGGCGCGCTCGACTTCATCCA
GAAGCCCTTCAACGAGGACGAACTCGTCAGCCTCGTGGACCGCATGCTCGACCGGGCCCGCGAGTCCTTCGCCGGCCACC
AGCAGGCCGCCAGCCGCGACGCCCTGCTCTCCAAGCTCACCGGGCGCGAAGCGCAGGTGCTCGAGCGCATCGTCGCCGGC
CGCCTGAACAAGCAGATCGCCGACGACCTGGGCATCAGCATCAAGACCGTGGAAGCGCACCGCGCCAACATCATGGAAAA
GCTGAACGCCAACACCGTGGCCGACCTGCTCAAGATCGCCCTGGGCCAGAACGCGCCCAAGTCCTGA

Upstream 100 bases:

>100_bases
AGGTCACCGGCTGCCGATTCTCCTTCTGGCTTCCGCTCGCCCAGCCTGCGGATGACACTACGAATTCTGTAGCAAAAGCA
TCTACCCCAAGGACTCCCGC

Downstream 100 bases:

>100_bases
CCGCCTCCGCAACGGCCGCCCGGTCCACCGGCGCCCTGCCGGGGCCGGCGGCCTTTTTCGCATCGAACGCACCGAAATCC
CCTTCCGACATGACCGCCCA

Product: two component LuxR family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MSLTPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVRCFDSAESFLSRYDPREVACLIVDIRMGGMTGLELQDRLLERKSPL
PIVFITGHGDVPMAVNTMKKGALDFIQKPFNEDELVSLVDRMLDRARESFAGHQQAASRDALLSKLTGREAQVLERIVAG
RLNKQIADDLGISIKTVEAHRANIMEKLNANTVADLLKIALGQNAPKS

Sequences:

>Translated_208_residues
MSLTPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVRCFDSAESFLSRYDPREVACLIVDIRMGGMTGLELQDRLLERKSPL
PIVFITGHGDVPMAVNTMKKGALDFIQKPFNEDELVSLVDRMLDRARESFAGHQQAASRDALLSKLTGREAQVLERIVAG
RLNKQIADDLGISIKTVEAHRANIMEKLNANTVADLLKIALGQNAPKS
>Mature_207_residues
SLTPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVRCFDSAESFLSRYDPREVACLIVDIRMGGMTGLELQDRLLERKSPLP
IVFITGHGDVPMAVNTMKKGALDFIQKPFNEDELVSLVDRMLDRARESFAGHQQAASRDALLSKLTGREAQVLERIVAGR
LNKQIADDLGISIKTVEAHRANIMEKLNANTVADLLKIALGQNAPKS

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=123, Percent_Identity=35.7723577235772, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786747, Length=184, Percent_Identity=25.5434782608696, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790102, Length=200, Percent_Identity=31.5, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788222, Length=205, Percent_Identity=26.8292682926829, Blast_Score=64, Evalue=6e-12,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=27.1929824561404, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789809, Length=124, Percent_Identity=33.0645161290323, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23138; Mature: 23007

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLTPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVRCFDSAESFLSRYDPREVACLIVDIR
CCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCEEEEEEEEE
MGGMTGLELQDRLLERKSPLPIVFITGHGDVPMAVNTMKKGALDFIQKPFNEDELVSLVD
ECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RMLDRARESFAGHQQAASRDALLSKLTGREAQVLERIVAGRLNKQIADDLGISIKTVEAH
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHH
RANIMEKLNANTVADLLKIALGQNAPKS
HHHHHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SLTPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVRCFDSAESFLSRYDPREVACLIVDIR
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCEEEEEEEEE
MGGMTGLELQDRLLERKSPLPIVFITGHGDVPMAVNTMKKGALDFIQKPFNEDELVSLVD
ECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RMLDRARESFAGHQQAASRDALLSKLTGREAQVLERIVAGRLNKQIADDLGISIKTVEAH
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEHHHH
RANIMEKLNANTVADLLKIALGQNAPKS
HHHHHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]