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The map label for this gene is folD

Identifier: 120611130

GI number: 120611130

Start: 2682267

End: 2683118

Strand: Reverse

Name: folD

Synonym: Aave_2459

Alternate gene names: 120611130

Gene position: 2683118-2682267 (Counterclockwise)

Preceding gene: 120611131

Following gene: 120611129

Centisome position: 50.13

GC content: 69.01

Gene sequence:

>852_bases
ATGACCGCCCAACTGATCGACGGCAACGCCCTTTCCCGACAACTCCGCGCCGACGTGGCCCGCCGCGCCGCGCTGCTCAA
GGAACGCGGCACCACGCCGGGCCTGGCCGTCGTGCTGGTGGGCGACAACCCCGCCAGCCAGGTGTACGTGCGCAACAAGG
TCAAGGCCTGCGAGGAAAGCGGCCTGCATTCGGTGCTCGAGAAGTACGACGCCGCGATGTCCGAGGCGGAGCTGCTGGCC
CGCGTGGACGCGCTGAACAACGACCCGTCCATCCACGGCATCCTGGTGCAGCTGCCCCTGCCCGCCCACATCGACGCGCA
GAAGGTCATCGAGGCGATCTCTCCGGCCAAGGACGTGGACGGCTTCCACATCGCCAGCGCCGGTGCCCTGATGACCGGCA
TGCCCGGCTTCTGGCCCTGCACGCCCTATGGCTGCATGAAGATGCTGGAGCACATCGGCTACGACCTGCGCGGCAAGCAC
GCCGTGGTGATCGGCCGCAGCAACATCGTGGGCAAGCCCATGGCGCTGATGCTGCTGGCCAAGGACGCCACCGTCACCGT
GTGCCACTCCCGCACCGCCGACCTCAAGGCCCAGACGCTGCAGGCGGACGTCATCGTCGCCGCCGTGGGCCGCCGCAACG
TGCTGACGGCCGACATGGTCAAGCCCGGCGCCGTCGTCATCGACGTGGGCATGAACCGCAACGACGAAGGCAGGCTCTGC
GGCGACGTGGACTTCGACGGGGTGCGCGAAGTCGCGGGCCACATCACCCCCGTTCCCGGCGGGGTCGGCCCGATGACCAT
TACGATGCTGCTCGTGAACACCCTCGAAGCCGCCGAGCGCGCAGCCGGCTGA

Upstream 100 bases:

>100_bases
CCAAGTCCTGACCGCCTCCGCAACGGCCGCCCGGTCCACCGGCGCCCTGCCGGGGCCGGCGGCCTTTTTCGCATCGAACG
CACCGAAATCCCCTTCCGAC

Downstream 100 bases:

>100_bases
CCGATACCATCGAGCCAGGCTATCGAAGAGATTGCGCAAGCCGCTTGAACCCATCGTCCCCGCCGCCATCTGAACGCGCA
TGAGCAATCCCCTCCTCGAC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTAQLIDGNALSRQLRADVARRAALLKERGTTPGLAVVLVGDNPASQVYVRNKVKACEESGLHSVLEKYDAAMSEAELLA
RVDALNNDPSIHGILVQLPLPAHIDAQKVIEAISPAKDVDGFHIASAGALMTGMPGFWPCTPYGCMKMLEHIGYDLRGKH
AVVIGRSNIVGKPMALMLLAKDATVTVCHSRTADLKAQTLQADVIVAAVGRRNVLTADMVKPGAVVIDVGMNRNDEGRLC
GDVDFDGVREVAGHITPVPGGVGPMTITMLLVNTLEAAERAAG

Sequences:

>Translated_283_residues
MTAQLIDGNALSRQLRADVARRAALLKERGTTPGLAVVLVGDNPASQVYVRNKVKACEESGLHSVLEKYDAAMSEAELLA
RVDALNNDPSIHGILVQLPLPAHIDAQKVIEAISPAKDVDGFHIASAGALMTGMPGFWPCTPYGCMKMLEHIGYDLRGKH
AVVIGRSNIVGKPMALMLLAKDATVTVCHSRTADLKAQTLQADVIVAAVGRRNVLTADMVKPGAVVIDVGMNRNDEGRLC
GDVDFDGVREVAGHITPVPGGVGPMTITMLLVNTLEAAERAAG
>Mature_282_residues
TAQLIDGNALSRQLRADVARRAALLKERGTTPGLAVVLVGDNPASQVYVRNKVKACEESGLHSVLEKYDAAMSEAELLAR
VDALNNDPSIHGILVQLPLPAHIDAQKVIEAISPAKDVDGFHIASAGALMTGMPGFWPCTPYGCMKMLEHIGYDLRGKHA
VVIGRSNIVGKPMALMLLAKDATVTVCHSRTADLKAQTLQADVIVAAVGRRNVLTADMVKPGAVVIDVGMNRNDEGRLCG
DVDFDGVREVAGHITPVPGGVGPMTITMLLVNTLEAAERAAG

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=43.1034482758621, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=44.5578231292517, Blast_Score=227, Evalue=1e-59,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=43.5374149659864, Blast_Score=221, Evalue=7e-58,
Organism=Homo sapiens, GI36796743, Length=224, Percent_Identity=24.1071428571429, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=50.3597122302158, Blast_Score=260, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17568735, Length=294, Percent_Identity=42.5170068027211, Blast_Score=223, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=47.0383275261324, Blast_Score=259, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=41.6107382550336, Blast_Score=223, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=292, Percent_Identity=27.0547945205479, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=46.9387755102041, Blast_Score=251, Evalue=6e-67,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=46.9387755102041, Blast_Score=250, Evalue=6e-67,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=42.2145328719723, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=42.2145328719723, Blast_Score=238, Evalue=3e-63,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=42.2145328719723, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=42.2145328719723, Blast_Score=238, Evalue=4e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_ACIAC (A1TPZ6)

Other databases:

- EMBL:   CP000512
- RefSeq:   YP_970808.1
- ProteinModelPortal:   A1TPZ6
- SMR:   A1TPZ6
- STRING:   A1TPZ6
- GeneID:   4669827
- GenomeReviews:   CP000512_GR
- KEGG:   aav:Aave_2459
- NMPDR:   fig|397945.5.peg.2104
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- PhylomeDB:   A1TPZ6
- ProtClustDB:   PRK14189
- BioCyc:   AAVE397945:AAVE_2459-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 29882; Mature: 29751

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQLIDGNALSRQLRADVARRAALLKERGTTPGLAVVLVGDNPASQVYVRNKVKACEES
CCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEHHHHHHHHHC
GLHSVLEKYDAAMSEAELLARVDALNNDPSIHGILVQLPLPAHIDAQKVIEAISPAKDVD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHIASAGALMTGMPGFWPCTPYGCMKMLEHIGYDLRGKHAVVIGRSNIVGKPMALMLLA
CEEEECCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEEEE
KDATVTVCHSRTADLKAQTLQADVIVAAVGRRNVLTADMVKPGAVVIDVGMNRNDEGRLC
CCCEEEEEECCCCCCHHHHHHHEEEEEECCCCCCEEHHCCCCCEEEEEECCCCCCCCCEE
GDVDFDGVREVAGHITPVPGGVGPMTITMLLVNTLEAAERAAG
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TAQLIDGNALSRQLRADVARRAALLKERGTTPGLAVVLVGDNPASQVYVRNKVKACEES
CCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEHHHHHHHHHC
GLHSVLEKYDAAMSEAELLARVDALNNDPSIHGILVQLPLPAHIDAQKVIEAISPAKDVD
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHCCCCCCC
GFHIASAGALMTGMPGFWPCTPYGCMKMLEHIGYDLRGKHAVVIGRSNIVGKPMALMLLA
CEEEECCCHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEEEE
KDATVTVCHSRTADLKAQTLQADVIVAAVGRRNVLTADMVKPGAVVIDVGMNRNDEGRLC
CCCEEEEEECCCCCCHHHHHHHEEEEEECCCCCCEEHHCCCCCEEEEEECCCCCCCCCEE
GDVDFDGVREVAGHITPVPGGVGPMTITMLLVNTLEAAERAAG
CCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA