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The map label for this gene is lpdG [H]

Identifier: 120611104

GI number: 120611104

Start: 2654607

End: 2656019

Strand: Reverse

Name: lpdG [H]

Synonym: Aave_2433

Alternate gene names: 120611104

Gene position: 2656019-2654607 (Counterclockwise)

Preceding gene: 120611105

Following gene: 120611103

Centisome position: 49.62

GC content: 69.43

Gene sequence:

>1413_bases
ATGAGCGACACGCTGGACGTCATCATTCTCGGAGCAGGATCCGCCGGCCTCGCCGCGTTGCGCGAAGTGCGCAAGCGCAC
GGAGCGGGTGCTGATCGTGAACGACGGACCGTGGGGCACCACCTGCGCCCGCGTGGGGTGCATGCCGTCGAAGATGCTCA
TCGAGGCCGCCGACGCATTCCACCGCCGCCACAGTTTCGATACGTTCGGCATCCGCGGGGGACGGTCGCTGGGGCTGGAC
CTCCCGGCCGTGCTGGACCGGGTCCGCGCGCTGCGCGACGACTTCGTCGCGGGTACGCGCAAGGCGTCCGACATCGGCGC
CCAGGGCATTGCCGGGCACGCGCGGCTCATGGGCCCGCACCGCGTAGAGGTGGATGGCCGCGTGTACGACACCCGCAGCA
TCATCATCGCGACAGGTTCCCGGCCCATCGTGCCGGAAGAATGGCTGGCCTTCGGGGACCGCATCCTCACGACCGACACC
CTGTTCGAGCAGCGCGACCTGGGGCCGCGCATGGCGATCATTGGACTGGGGCCGCTGGGGGCGGAAATCGCCCAGGCGCT
CGCCCGGCTGGGCGTGGAGGTGGCGGCTTTCTCGAGCCGCAAGGAAATCGCCGGCCTCTCCGACCCCGCCGTGAACGACG
CGCTGCTCGGGCTGCTGAAATCGGAGTTCGTTCTGAACGTGGGCGCGGAGGTGACGCTGCGCGAGGTGCCGGGGGGCATC
GAGGTCACCAATGGCAGCGCCACGGTCGTGGTGGACCAGGTCCTCGCAGCCATGGGACGGCGCCCGAACGTGGAGCACCT
CGGGCTGGAAACGCTGGGCGTGGAACTGGACAAGGACGGCATGCCGCCGGTGGACCGGCGCACGGTGCAGGTCGGCGATC
TGCCGGTGTTCATGGCCGGAGATGCGAACGACTTCCGGCCCCTGCTGCACGAGGCGGCGGACGACGGGCACATCGCCGGG
CTGAATGCGCTGGCTCCCGAAGTGCGGGGGTTCCGTCGCCGTATGCCGCTGTCCATCGTATTCACGGAGCCCAACGCGGC
GACCGTCGGCAGGCGCTACAAGGACCTGAAGCGGGACGAATCGGTGACCGGCACCGTCGATTTCGCGCGCCAGGGGCGGG
CTCGCGTGGCGCAGCGCAACCAGGGACGCCTGAGCCTCTACGCCCAGCGGGACACAGGCCGGCTGCTGGGCGCGGAAATG
TGCGCCCCGGCGGGCGAGCACATGGCCCACCTGCTGGCGCTGGCCATGGACCGGGAACTCACCGTGCATGACCTCCTGCG
GATGCCTTTCTACCATCCTGTACTGGAAGAAGGACTGCGGAGCGCACTACGAGACGCCGCCAGCCAGTTGCCCAAGGGCA
GCGATTCCGATCTTTCCGCCTGCGACGCCTATGGGTCGAGCGCCCTGGACTGA

Upstream 100 bases:

>100_bases
GGCGGCGAGGCAAGACAGCCTTTCCGCCTACATGCCGCCATGCATGCGGGCGCAGACACTTTCCTGCCGAGCCACACACC
TGAGCAGGAGACCTAGGCCC

Downstream 100 bases:

>100_bases
CGCCCCGGCATCGCGCCAGACCGGCCCATCAAGATGTAACAGTACTCAAGGCGGCCTGGAAAGCGTCCGATAACGGGGAA
GCATTTGCAAACCCGGCCTC

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: E3 component of 2-oxoglutarate dehydrogenase complex; Glycine oxidation system L-factor; LPD-GLC [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSDTLDVIILGAGSAGLAALREVRKRTERVLIVNDGPWGTTCARVGCMPSKMLIEAADAFHRRHSFDTFGIRGGRSLGLD
LPAVLDRVRALRDDFVAGTRKASDIGAQGIAGHARLMGPHRVEVDGRVYDTRSIIIATGSRPIVPEEWLAFGDRILTTDT
LFEQRDLGPRMAIIGLGPLGAEIAQALARLGVEVAAFSSRKEIAGLSDPAVNDALLGLLKSEFVLNVGAEVTLREVPGGI
EVTNGSATVVVDQVLAAMGRRPNVEHLGLETLGVELDKDGMPPVDRRTVQVGDLPVFMAGDANDFRPLLHEAADDGHIAG
LNALAPEVRGFRRRMPLSIVFTEPNAATVGRRYKDLKRDESVTGTVDFARQGRARVAQRNQGRLSLYAQRDTGRLLGAEM
CAPAGEHMAHLLALAMDRELTVHDLLRMPFYHPVLEEGLRSALRDAASQLPKGSDSDLSACDAYGSSALD

Sequences:

>Translated_470_residues
MSDTLDVIILGAGSAGLAALREVRKRTERVLIVNDGPWGTTCARVGCMPSKMLIEAADAFHRRHSFDTFGIRGGRSLGLD
LPAVLDRVRALRDDFVAGTRKASDIGAQGIAGHARLMGPHRVEVDGRVYDTRSIIIATGSRPIVPEEWLAFGDRILTTDT
LFEQRDLGPRMAIIGLGPLGAEIAQALARLGVEVAAFSSRKEIAGLSDPAVNDALLGLLKSEFVLNVGAEVTLREVPGGI
EVTNGSATVVVDQVLAAMGRRPNVEHLGLETLGVELDKDGMPPVDRRTVQVGDLPVFMAGDANDFRPLLHEAADDGHIAG
LNALAPEVRGFRRRMPLSIVFTEPNAATVGRRYKDLKRDESVTGTVDFARQGRARVAQRNQGRLSLYAQRDTGRLLGAEM
CAPAGEHMAHLLALAMDRELTVHDLLRMPFYHPVLEEGLRSALRDAASQLPKGSDSDLSACDAYGSSALD
>Mature_469_residues
SDTLDVIILGAGSAGLAALREVRKRTERVLIVNDGPWGTTCARVGCMPSKMLIEAADAFHRRHSFDTFGIRGGRSLGLDL
PAVLDRVRALRDDFVAGTRKASDIGAQGIAGHARLMGPHRVEVDGRVYDTRSIIIATGSRPIVPEEWLAFGDRILTTDTL
FEQRDLGPRMAIIGLGPLGAEIAQALARLGVEVAAFSSRKEIAGLSDPAVNDALLGLLKSEFVLNVGAEVTLREVPGGIE
VTNGSATVVVDQVLAAMGRRPNVEHLGLETLGVELDKDGMPPVDRRTVQVGDLPVFMAGDANDFRPLLHEAADDGHIAGL
NALAPEVRGFRRRMPLSIVFTEPNAATVGRRYKDLKRDESVTGTVDFARQGRARVAQRNQGRLSLYAQRDTGRLLGAEMC
APAGEHMAHLLALAMDRELTVHDLLRMPFYHPVLEEGLRSALRDAASQLPKGSDSDLSACDAYGSSALD

Specific function: Also acts in the glycine cleavage system [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=462, Percent_Identity=29.2207792207792, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI50301238, Length=480, Percent_Identity=25.8333333333333, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1786307, Length=477, Percent_Identity=26.4150943396226, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87082354, Length=483, Percent_Identity=22.7743271221532, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=23.5697940503432, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=24.7787610619469, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=28.1385281385281, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71983419, Length=294, Percent_Identity=27.2108843537415, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71983429, Length=295, Percent_Identity=27.1186440677966, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=25.369978858351, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6325240, Length=475, Percent_Identity=22.9473684210526, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6325166, Length=471, Percent_Identity=22.7176220806794, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI21358499, Length=472, Percent_Identity=28.3898305084746, Blast_Score=145, Evalue=9e-35,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50535; Mature: 50404

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTLDVIILGAGSAGLAALREVRKRTERVLIVNDGPWGTTCARVGCMPSKMLIEAADAF
CCCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHH
HRRHSFDTFGIRGGRSLGLDLPAVLDRVRALRDDFVAGTRKASDIGAQGIAGHARLMGPH
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHCCCCE
RVEVDGRVYDTRSIIIATGSRPIVPEEWLAFGDRILTTDTLFEQRDLGPRMAIIGLGPLG
EEEECCEEEECCEEEEECCCCCCCCHHHHHCCCEEEEHHHHHHHHCCCCEEEEEECCCCH
AEIAQALARLGVEVAAFSSRKEIAGLSDPAVNDALLGLLKSEFVLNVGAEVTLREVPGGI
HHHHHHHHHCCCHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHEECCCEEEEEECCCCE
EVTNGSATVVVDQVLAAMGRRPNVEHLGLETLGVELDKDGMPPVDRRTVQVGDLPVFMAG
EEECCCHHHHHHHHHHHHCCCCCHHHCCHHHHCCEECCCCCCCCCCCEEEECCEEEEEEC
DANDFRPLLHEAADDGHIAGLNALAPEVRGFRRRMPLSIVFTEPNAATVGRRYKDLKRDE
CCCHHHHHHHHCCCCCCEECHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCC
SVTGTVDFARQGRARVAQRNQGRLSLYAQRDTGRLLGAEMCAPAGEHMAHLLALAMDREL
CCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHCCCC
TVHDLLRMPFYHPVLEEGLRSALRDAASQLPKGSDSDLSACDAYGSSALD
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCC
>Mature Secondary Structure 
SDTLDVIILGAGSAGLAALREVRKRTERVLIVNDGPWGTTCARVGCMPSKMLIEAADAF
CCCEEEEEEECCCHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHH
HRRHSFDTFGIRGGRSLGLDLPAVLDRVRALRDDFVAGTRKASDIGAQGIAGHARLMGPH
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHCCCCE
RVEVDGRVYDTRSIIIATGSRPIVPEEWLAFGDRILTTDTLFEQRDLGPRMAIIGLGPLG
EEEECCEEEECCEEEEECCCCCCCCHHHHHCCCEEEEHHHHHHHHCCCCEEEEEECCCCH
AEIAQALARLGVEVAAFSSRKEIAGLSDPAVNDALLGLLKSEFVLNVGAEVTLREVPGGI
HHHHHHHHHCCCHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHEECCCEEEEEECCCCE
EVTNGSATVVVDQVLAAMGRRPNVEHLGLETLGVELDKDGMPPVDRRTVQVGDLPVFMAG
EEECCCHHHHHHHHHHHHCCCCCHHHCCHHHHCCEECCCCCCCCCCCEEEECCEEEEEEC
DANDFRPLLHEAADDGHIAGLNALAPEVRGFRRRMPLSIVFTEPNAATVGRRYKDLKRDE
CCCHHHHHHHHCCCCCCEECHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHCCC
SVTGTVDFARQGRARVAQRNQGRLSLYAQRDTGRLLGAEMCAPAGEHMAHLLALAMDREL
CCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHCCCC
TVHDLLRMPFYHPVLEEGLRSALRDAASQLPKGSDSDLSACDAYGSSALD
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]